IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Hypoxia-responsive miRNAs target argonaute 1 to promote angiogenesis. Hypoxia-responsive miRNAs target argonaute 1 to promote angiogenesis. The Journal of clinical investigation 2013 imex curation Chen Z., Lai TC., Jan YH., Lin FM., Wang WC., Xiao H., Wang YT., Sun W., Cui X., Li YS., Fang T., Zhao H., Padmanabhan C., Sun R., Wang DL., Jin H., Chau GY., Huang HD., Hsiao M., Shyy JY. 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. mirna interference luciferase assay miRNA interference luciferase reporter assay miRNA interference luciferase assay tag luciferase tag visualisation by luciferase assay tag luciferase Chen Z., Lai TC., Jan YH., Lin FM., Wang WC., Xiao H., Wang YT., Sun W., Cui X., Li YS., Fang T., Zhao H., Padmanabhan C., Sun R., Wang DL., Jin H., Chau GY., Huang HD., Hsiao M., Shyy JY. I did not add the mutation in 3' UTR that diminishes the binding because I may guess where it is for microRNA103 interaction but I have no idea what they delete for Let7 interaction. Accepted 2018-MAY-30 AT 11:46 BST AT 11:46 BST by LIVIAP The Journal of clinical investigation 2013 imex curation hsa-pre-let7e microRNA let7 e precursor mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human CCCGGGCUGAGGUAGGAGGUUGUAUAGUUGAGGAGGACACCCAAGGAGAUCACUAUACGGCCUCCUAGCUUUCCCCAGG mrna_ago1 mRNA argonaute RISC catalytic component 1 mrna messenger rna mRNA mrna human Homo sapiens Human GGTCGCGCCTGCGCACTGGCAGCTGGCCGGGCGCTCGCAGTGGGAGCTGCTGCAGGCTCCGCGGCGGCGGCAACGGAGGCTGCGGGGGCGGCGGCGCGAGCGGCCGGGCTTGGTAGGGGAGCCGAGCCCGGCCCGGGATCCCGAGCAGCGAGAGTGTGGGGTACCTAGGCCCCTCACGCTGGACTTCACAGTCTCCGGGCCGCCTGACCTCCGCACGGGTATATGGGATGGAAGCGGGACCCTCGGGAGCAGCTGCGGGCGCTTACCTGCCCCCCCTGCAGCAGGTGTTCCAGGCACCTCGCCGGCCTGGCATTGGCACTGTGGGGAAACCAATCAAGCTCCTGGCCAATTACTTTGAGGTGGACATCCCTAAGATCGACGTGTACCACTACGAGGTGGACATCAAGCCGGATAAGTGTCCCCGTAGAGTCAACCGGGAAGTGGTGGAATACATGGTCCAGCATTTCAAGCCTCAGATCTTTGGTGATCGCAAGCCTGTGTATGATGGAAAGAAGAACATTTACACTGTCACAGCACTGCCCATTGGCAACGAACGGGTCGACTTTGAGGTGACAATCCCTGGGGAAGGGAAGGATCGAATCTTTAAGGTCTCCATCAAGTGGCTAGCCATTGTGAGCTGGCGAATGCTGCATGAGGCCCTGGTCAGCGGCCAGATCCCTGTTCCCTTGGAGTCTGTGCAAGCCCTGGATGTGGCCATGAGGCACCTGGCATCCATGAGGTACACCCCTGTGGGCCGCTCCTTCTTCTCACCGCCTGAGGGCTACTACCACCCGCTGGGGGGTGGGCGCGAGGTCTGGTTCGGCTTTCACCAGTCTGTGCGCCCTGCCATGTGGAAGATGATGCTCAACATTGATGTCTCAGCCACTGCCTTTTATAAGGCACAGCCAGTGATTGAGTTCATGTGTGAGGTGCTGGACATCAGGAACATAGATGAGCAGCCCAAGCCCCTCACGGACTCTCAGCGCGTTCGCTTCACCAAGGAGATCAAGGGCCTGAAGGTGGAAGTCACCCACTGTGGACAGATGAAGAGGAAGTACCGCGTGTGTAATGTTACCCGTCGCCCTGCTAGCCATCAGACATTCCCCTTACAGCTGGAGAGTGGACAGACTGTGGAGTGCACAGTGGCACAGTATTTCAAGCAGAAATATAACCTTCAGCTCAAGTATCCCCATCTGCCCTGCCTACAAGTTGGCCAGGAACAAAAGCATACCTACCTTCCCCTAGAGGTCTGTAACATTGTGGCTGGGCAGCGCTGTATTAAAAAGCTGACCGACAACCAGACCTCGACCATGATAAAGGCCACAGCTAGATCCGCTCCAGACAGACAGGAGGAGATCAGTCGCCTGATGAAGAATGCCAGCTACAACTTAGATCCCTACATCCAGGAATTTGGGATCAAAGTGAAGGATGACATGACGGAGGTGACAGGGCGAGTGCTGCCGGCGCCCATCTTGCAGTACGGCGGCCGGAACCGGGCCATTGCCACACCCAATCAGGGTGTCTGGGACATGCGGGGGAAACAGTTCTACAATGGGATTGAGATCAAAGTCTGGGCCATCGCCTGCTTCGCACCCCAAAAACAGTGTCGAGAAGAGGTGCTCAAGAACTTCACAGACCAGCTGCGGAAGATTTCCAAGGATGCGGGGATGCCTATCCAGGGTCAACCTTGTTTCTGCAAATATGCACAGGGGGCAGACAGCGTGGAGCCTATGTTCCGGCATCTCAAGAACACCTACTCAGGGCTGCAGCTCATTATTGTCATCCTGCCAGGGAAGACGCCGGTGTATGCTGAGGTGAAACGTGTCGGAGATACACTCTTGGGAATGGCTACGCAGTGTGTGCAGGTGAAGAACGTGGTCAAGACCTCACCTCAGACTCTGTCCAACCTCTGCCTCAAGATCAATGTCAAACTTGGTGGCATTAACAACATCCTAGTCCCACACCAGCGCTCTGCCGTTTTTCAACAGCCAGTGATATTCCTGGGAGCAGATGTTACACACCCCCCAGCAGGGGATGGGAAAAAACCTTCTATCACAGCAGTGGTAGGCAGTATGGATGCCCACCCCAGCCGATACTGTGCTACTGTGCGGGTACAGCGACCACGGCAAGAGATCATTGAAGACTTGTCCTACATGGTGCGTGAGCTCCTCATCCAATTCTACAAGTCCACCCGTTTCAAGCCTACCCGCATCATCTTCTACCGAGATGGGGTGCCTGAAGGCCAGCTACCCCAGATACTCCACTATGAGCTACTGGCCATTCGTGATGCCTGCATCAAACTGGAAAAGGACTACCAGCCTGGGATCACTTATATTGTGGTGCAGAAACGCCATCACACCCGCCTTTTCTGTGCTGACAAGAATGAGCGAATTGGGAAGAGTGGTAACATCCCAGCTGGGACCACAGTGGACACCAACATCACCCACCCATTTGAGTTTGACTTCTATCTGTGCAGCCACGCAGGCATCCAGGGCACCAGCCGACCATCCCATTACTATGTTCTTTGGGATGACAACCGTTTCACAGCAGATGAGCTCCAGATCCTGACGTACCAGCTGTGCCACACTTACGTACGATGCACACGCTCTGTCTCTATCCCAGCACCTGCCTACTATGCCCGCCTGGTGGCTTTCCGGGCACGATACCACCTGGTGGACAAGGAGCATGACAGTGGAGAGGGGAGCCACATATCGGGGCAGAGCAATGGGCGGGACCCCCAGGCCCTGGCCAAAGCCGTGCAGGTTCACCAGGATACTCTGCGCACCATGTACTTCGCTTGAAGGCAGAACGCTGTTACCTCACTGGATAGAAGAAAGCTTTCCAAGCCCCAGGAGCTGTGCCACCCAAATCCAGAGGAAGCAAGGAGGAGGGAGGTGGGGTAGGGAGGAGTGTAGGATGCCTTGTTTCCTTCTATAGAGGTGGTGTAAGAGTGGGGAACAGGGCCAGCAAGACAGACCACCAGCCAGAAATCTCTGATATCAACCTCATGTCCCCCACCCCTCACCCCATCTTGTCACATCTGGCCCTGACCCCACTGGACCAAAAGGGGCAGCACTGGTGCCCACCATACACACAGGTGTCTCATGTGACTCACAGTGCTAAAGACTCATGCTTGACAGCTTGGTAAGGTCAACTCTGTAGCCCTGCAGACAAAAGCTGGTTAGGTTTGGGTTTGATACTTTAGATGGGAAAGTGAGGGGCTTGAGAAAGTGGGTGGGAGGAGGGAAGGATTTTTTAGGAGCCTTAATCAGAAAAGGACTAGATTTGTTTAAGAAGAAAAATGAAACCAGACCCAGATCAATATTTTAGGATACTAGATGTTTTAATGGGTTCAGAATCCAGTTTGTAGGAAGATTTTTTAATGGTTTTGGTTGCTCCTCCCCCAGCTGCCACCCCCCACCTTACCCTTATTCCTCTCTGTCCACATTTTCTGCCCCACCTTACTTCTCCTCCCTGACAGACATCCAGCCCCTAGTAATACTTAAGGCACTATGGCACTTAGCTTTGAAGTGACACGACCCTGTCTTCCTTCCGCCCGCTGGTGGGTAACCAGTGCCTTCCCTGTAACGGTAATGCTGCAGAACTGCAACCTTTTGTACCTTTCTTTGGGGAATGGGGTGGGGGTGGGAGAGGAGGTAGATGGGGAAGAAATACCCCAGACCCAACAAACCTCCAGCCAGAAAGCCAGCTATTTTGCATTTGAAGGAATTGACTTCCTCATTCATTGAGCTTTTTAAAAGATCACAACCTCAAGATGGTTAAAATCCATTGACATTTGCACTTTCAAACATGACAAGTCTCGGAGCTGCTGAGATGACAGGCCCCTGGCCTTTCCACTTATGCCTCCTTTTCTCCTTATTCCTCCTACCTCCCGCCCCGCCCAGGTCTGGAGTTACTTTCATAGCATTTTTCACTCTTGGCTTCTTTTCTCCCTTGATGGTCAAGTCTCTTATGTTTCAATATTTCTTAACTGGGGTGTCTTATAACAAAAAACTCTTAGGTCTAAAATGAGAAAAAAGAGAGAAAACAAAATGTTATTTTTATACCATAACTTGAGTGTATTGCCAAAATTTGGAAATCCTTCCCATGCCTGATGAGTTTATATCCCAGAAACATTGAGCCATCAGAATGAACTGTGTACCTGATTTGTTCTCTGACCTGGCTAGGTAGGGAGGGGGTGGTTATCGCCCCAAGATGGGGTCCAGGCTCCATCCTTCCTCTGTGCAGATAATACCTTTTTCTTGCTATAGCCTCCCTCCTCTGCACTGTCCTGCACTCTTTCTTGCAAGTGCATCTTTTTCCTTCCCCTGGACTGTCCTCTGACCCTTTGGCTCATCCTAGATTGCAGTGTGTCCTGTGGACAGGCTGGGGAATTTTGCTGCTCCCTATTGCTTCTGTTTACAAAAATGAATTTTTCCTGGTTTCCCACTAGGGCATGTGGGTGGGTGGCATGGACTTTTTTTTTTTTTTTTTTTTGTCTTGAGACATGGGGTTTGGCTGTCTTGCAGGACTGGAGAAGGTGGTGGTTCTAGCTTGGTCTCTGTTGGCCTTGAAGCAAGCATCCCCCCTGCCCTTTTTCCTTGACTGTTCATTTTTTTCCTGCCCCACTGCTTGGGATGGGGAGTTGCAACTTCAGTGTGGAATTTCCTCTTTGAGGAGCCTGGGCTTGGATCTATCCTGATCTGGTGATGAAGCCATGATTACTTTAGACCTAGCCCAGGCTTGGAGGCCAGCTGGAGGAAGAAGGGTCTAAATCCTGGCCTGTAGAGTTAGAACTACCATTTCCTCCCCTTAGCTGCCCTTGTATGACCCGGATTTGCTATGCAAAACAATCTATCCCAGGTTCTGTTCTGGTTGGCTACATTGTTCAGCAACTCACAAAACGTAGCACAAACATTCATTATGGAGAAAGCATCAGGACTGTTGAGTAACTCCTCCTTTACTTTTTTCCTGCTGGCTACAGCATGGGGTGCCCTATAGGCACAAGCCCAGCTGAAGAACAGAATGGAGGGCTCTGGGAGGAGGCAGCTCACTGGAGAGCCTACATTCCTTACACAAGTGCCTAAAGAGAGTGATGCTAACACTCCATCTGCCCTGTCCATTGCCTTCATATACAGTCTACTTCGTGTTCTGTCACCCTTTGGGGAGGGGAGTTCTCCTGGGACAGTGGGCTCTGCATGTTCTCCACTTGGATACATTTTGGGGCTAGGATCAGGGCACTATTCCTGGAGGGTCCAGTCATTCACCAGCATTTGCAAATGTCCATAGGGAGCAGGTGGCAGCCTCTACTCCCAGCAACAAGTTTGTGTTCTCTCCTTTTCTCTCTTTGCCTCACTCTCTCCAGTTGGTTTTCAGCTGGGGCTTGAAATGCATTTTTAGCCCTTTGACGTGGCTTATGCCATTCAAGAAATAAAAAGCAAGAGAATCAGCTTTGGGCAATGACAAGAAATGAGTTCTTACTCTGATTTTTTTGTAAAAAGATAATTTTTGAGACTTGAAAAATACCCCGACCTTGAGATTATTCCTGTTTGAAAGGTGGTGCATGCAGATGGAGAAGTGGTGTTGGCAGCAAGCTTTGGCTCATGTGGATTTGGTTTAAGTGGTGCTTCTTACCCAAGCTTCAAGGAAGTGCTTGGGGGACCCCCAGCCTCATCCTCTTAGTTGGGTCTCTTGTTCCCTTTGTACCACTGTTTTGCCTTCCTTTTCCTCTTCTCTCTTTGCCTGGCTTCCTTTCCCTTTTCTTCTATTCACTCTGCTTGCTTGCTGGCCGGCCTGCCTGCCTGCCTGCCTGCCTGCCTGCCTGTCTGCCTATGTGATGATGAAATCTCTGCATGGCTGCAATGATCCCACTGTTAGCTGGCAGGGTCAGGCTTAGCTCCTTGACTGCAGAAGACCAAGAACCTGTTCCCCAAGCCCAGAGATGTCCACCTGGGCTGGACTGCCCTCAAGCTTATACTAGAGAAGAGCAACTGACCTGCCCAACTTGTGTGAAGTCAGGAGGGTTTCTGGCATTTTCCACACCTGTCCACTCCTTGGAGCTGGTTTCTCTCATTGCTTTTTCTAAATCTGGTTCTTTTTCTCTTTACCTGGGGCCTGGCTTTTCTGAGATTGTCTTAGGGTTGAGCTATTTGGGTATCCTGGGTTTGAGTGTTAGGGGATGGACATAAAGGAAAAAGAGTGATGAGAAGAGAATGGAGAGAATTTGAATAAAAGGTGGGAAAGGAGAGCACTGTTCTTTGATTGTTTATCCAGTCCAACCTGATCCATTAGGGATCGAGGTGCTACACTGGCCTCCAGGGATAAGCCTGGGGCTACTGTTGCTGGGAACTTAGGCTTAACATAAAGCCGAAGAAGGTACCTAGAAATTTGAAACTTCCCTAAAAAGCTCCTAATGCCCACCTGCTAGATAGCTTCTCTGTGGCCTCCTATTTAGCTAAGCAGCAGTGTTTTTGGATACTTTTTTTTTCTGTTTGTGAATAAGGCCAGCACTCAAGATGGGCAGCCAAGGGTGCACTGACTATTAGCTGGCCCATAGGATATCTGTAAGGCTGGTGGGACAGTTTTGGACCTGGAATCATGTGTAACTAACAAGGTTGGACGTTTCTTCCCCATCAGGGTAGAAAAATCATCTCAAACTAGCCAAAAGGCAGTTTTGGAAACTACATTGGGGGACGTTATTTTTATTTATATATGGGGCCTAGGCCAATCCAGGATGGTAGCTGGAATACCTTCCTTCTTAAAATCTGATCATGGCAGGGATATGCAGGGCACTTTTTACTATTTGGCCTTCTAAGCAGATTGGGAAGGAGGTATTTTCTGGTTTTCGCTTTCCTCCGACTTAATAGGACTTGCCTTCTCCCTGGGCAGGGAGAGAGGCTGGGTTGGTGCTCTCCCTTACTCTACTCATACTGACTTAGAGCCTCTGGCTGCTGTTTGGGCATCCAAGAAAGGGAGGGGAAGGAATGAGCTAAAAACAAAACAGAATGAGGTGGGAAAGGGAGATTTTCTTCTTTACAGAGGAAAATAGGAAACCCTCCAAGAATTGTGCAAGTAAAGACATTTGTTGAATGCACTGAGTCCCTTGGTGTAGTAGCAATAAGGAAAAATGAAATTACTTTCCTGTGCACACAGTCCAGCCTAATTGGTATGTGATGTTGCACTTAGCAGCCATGTGGTGGGCATGTGTGACTACTCTGGTTTTCACTTTAGTTTCTAAACTTTTTATCCCTCTCAAGTCCAGCATGGATGGGGAAATGTCTCTGGATCCCCACAGCTGTGTACTTGTTTGCATTTGTTTCCCTTTGAGATTTGTGTTTGTGTCCTGCTTTGAGCTGTACCTTGTCCAGTCCATTGTGAAATTATCCCAGCAGCTGTAATGTACAGTTCCTTCTGAAGCAAGCAACATCAGCAGCAGCAGCAGCAGCAGCACAATTCTGTGTTTTATAAAGACAACAGTGGCTTCTATTTCTAAAAAAAAAAAAAAAAAAAA hsa-pre-mir103 microRNA 103 precursor mirna micro rna micro RNA micro-RNA micro-rna mirna miRNA human Homo sapiens Human UACUGCCCUCGGCUUCUUUACAGUGCUGCCUUGUUGCAUAUGGAUCAAGCAGCAUUGUACAGGGCUAUGAAGGCAUUG mrna_ago1-pre_let7 e-1 1 2 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered 3 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 3a mrna_ago1-pre_mir 103-1 1 4 predetermined predetermined participant predetermined tag luciferase tag visualisation by luciferase assay tag luciferase regulator regulator modulator regulator neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered synthetic construct synthetic construct 3 regulator target regulator target regulator target modulator target neutral component neutral component over-expressed over expressed level over-expressed living cell living cell engineered engineered tag luciferase tag luciferase tag certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 3a