IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Communication between the ERRalpha homodimer interface and the PGC-1alpha binding surface via the helix 8-9 loop. Communication between the ERRalpha homodimer interface and the PGC-1alpha binding surface via the helix 8-9 loop. The Journal of biological chemistry 2008 imex curation Greschik H., Althage M., Flaig R., Sato Y., Chavant V., Peluso-Iltis C., Choulier L., Cronet P., Rochel N., Schüle R., Strömstedt PE., Moras D. moras@igbmc.u-strasbg.fr. 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Greschik H., Althage M., Flaig R., Sato Y., Chavant V., Peluso-Iltis C., Choulier L., Cronet P., Rochel N., Schüle R., Strömstedt PE., Moras D. moras@igbmc.u-strasbg.fr. Accepted 2018-JUN-22 AT 12:51 BST AT 12:51 BST by PPORRAS The Journal of biological chemistry 2008 Only protein-protein interactions imex curation imex curation Communication between the ERRalpha homodimer interface and the PGC-1alpha binding surface via the helix 8-9 loop. Communication between the ERRalpha homodimer interface and the PGC-1alpha binding surface via the helix 8-9 loop. The Journal of biological chemistry 2008 imex curation Greschik H., Althage M., Flaig R., Sato Y., Chavant V., Peluso-Iltis C., Choulier L., Cronet P., Rochel N., Schüle R., Strömstedt PE., Moras D. moras@igbmc.u-strasbg.fr. 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 gal4 vp16 complement gal4 vp16 complementation karyoplasmic interaction ion strategy KISS mammalian two hybrid gal4 vp16 complement predetermined predetermined participant predetermined Greschik H., Althage M., Flaig R., Sato Y., Chavant V., Peluso-Iltis C., Choulier L., Cronet P., Rochel N., Schüle R., Strömstedt PE., Moras D. Some results from figure 3B and 5B have not been included as chimeric constructs are used, which are of dubious biological relevance. I have not curated most results (middle 2 bars) from fig 3B as the alterations are quite large, apart from the bottom construct which has only one aa difference- but I HAVE added the "chimeric" mutated "swop" construct in fig 4C as the mutations cover a much smaller sequence. Not added bigger mutations including "swops" from fig 5D as very big/relevance? moras@igbmc.u-strasbg.fr. Accepted 2018-JUN-22 AT 12:51 BST AT 12:51 BST by PPORRAS The Journal of biological chemistry 2008 Only protein-protein interactions imex curation imex curation Communication between the ERRalpha homodimer interface and the PGC-1alpha binding surface via the helix 8-9 loop. Communication between the ERRalpha homodimer interface and the PGC-1alpha binding surface via the helix 8-9 loop. The Journal of biological chemistry 2008 imex curation Greschik H., Althage M., Flaig R., Sato Y., Chavant V., Peluso-Iltis C., Choulier L., Cronet P., Rochel N., Schüle R., Strömstedt PE., Moras D. moras@igbmc.u-strasbg.fr. 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Greschik H., Althage M., Flaig R., Sato Y., Chavant V., Peluso-Iltis C., Choulier L., Cronet P., Rochel N., Schüle R., Strömstedt PE., Moras D. it is difficult to work out the effect of some of these mutations - text not overly helpful- the E343A looks like a "mutation increasing" for the ERRa dimer, but I cannot find any mention of this in the text so have left as "mutation of NO effect". moras@igbmc.u-strasbg.fr. Accepted 2018-JUN-22 AT 12:51 BST AT 12:51 BST by PPORRAS The Journal of biological chemistry 2008 Only protein-protein interactions imex curation imex curation err1_human Steroid hormone receptor ERR1 Estrogen-related receptor alpha Estrogen receptor-like 1 Nuclear receptor subfamily 3 group B member 1 ESRRA ERR1 ESRL1 NR3B1 protein protein human Homo sapiens Human MSSQVVGIEPLYIKAEPASPDSPKGSSETETEPPVALAPGPAPTRCLPGHKEEEDGEGAGPGEQGGGKLVLSSLPKRLCLVCGDVASGYHYGVASCEACKAFFKRTIQGSIEYSCPASNECEITKRRRKACQACRFTKCLRVGMLKEGVRLDRVRGGRQKYKRRPEVDPLPFPGPFPAGPLAVAGGPRKTAAPVNALVSHLLVVEPEKLYAMPDPAGPDGHLPAVATLCDLFDREIVVTISWAKSIPGFSSLSLSDQMSVLQSVWMEVLVLGVAQRSLPLQDELAFAEDLVLDEEGARAAGLGELGAALLQLVRRLQALRLEREEYVLLKALALANSDSVHIEDAEAVEQLREALHEALLEYEAGRAGPGGGAERRRAGRLLLTLPLLRQTAGKVLAHFYGVKLEGKVPMHKLFLEMLEAMMD DIP protein P11474 original sequence version: 102 BAE62DAF0BE6BA96 esr1_human Estrogen receptor ESR1 ESR NR3A1 Estradiol receptor ER-alpha Nuclear receptor subfamily 3 group A member 1 protein protein human Homo sapiens Human MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV DIP protein P03372 original sequence version: 214 DIP protein P03372 original sequence version: 139 DIP protein P03372 original sequence version: 167 5455C57AB0CCCAA7 dr0_probe direct repeat oligo with 0 bp spacing ds dna double stranded deoxyribonucleic acid ds DNA ds dna chemical synthesis Chemical synthesis (Chemical synthesis) AGCTTCAGGTCAAGGTCAGAGAGCT ere_probe oestrogen response element ds dna double stranded deoxyribonucleic acid ds DNA ds dna chemical synthesis Chemical synthesis (Chemical synthesis) GATCCGTCAGGTCACAGTGACCTGATACATC prgc1_human Peroxisome proliferator-activated receptor gamma coactivator 1-alpha Ligand effect modulator 6 PPARGC1A LEM6 PGC1 PGC1A PPARGC1 protein protein human Homo sapiens Human MAWDMCNQDSESVWSDIECAALVGEDQPLCPDLPELDLSELDVNDLDTDSFLGGLKWCSDQSEIISNQYNNEPSNIFEKIDEENEANLLAVLTETLDSLPVDEDGLPSFDALTDGDVTTDNEASPSSMPDGTPPPQEAEEPSLLKKLLLAPANTQLSYNECSGLSTQNHANHNHRIRTNPAIVKTENSWSNKAKSICQQQKPQRRPCSELLKYLTTNDDPPHTKPTENRNSSRDKCTSKKKSHTQSQSQHLQAKPTTLSLPLTPESPNDPKGSPFENKTIERTLSVELSGTAGLTPPTTPPHKANQDNPFRASPKLKSSCKTVVPPPSKKPRYSESSGTQGNNSTKKGPEQSELYAQLSKSSVLTGGHEERKTKRPSLRLFGDHDYCQSINSKTEILINISQELQDSRQLENKDVSSDWQGQICSSTDSDQCYLRETLEASKQVSPCSTRKQLQDQEIRAELNKHFGHPSQAVFDDEADKTGELRDSDFSNEQFSKLPMFINSGLAMDGLFDDSEDESDKLSYPWDGTQSYSLFNVSPSCSSFNSPCRDSVSPPKSLFSQRPQRMRSRSRSFSRHRSCSRSPYSRSRSRSPGSRSSSRSCYYYESSHYRHRTHRNSPLYVRSRSRSPYSRRPRYDSYEEYQHERLKREEYRREYEKRESERAKQRERQRQKAIEERRVIYVGKIRPDTTRTELRDRFEVFGEIEECTVNLRDDGDSYGFITYRYTCDAFAALENGYTLRRSNETDFELYFCGRKQFFKSNYADLDSNSDDFDPASTKSKYDSLDFDSLLKEAQRSLRR DIP protein Q9UBK2 original sequence version: 127 DIP protein Q9UBK2 original sequence version: 104 97DDD3643051A5F1 12345rtyuuuu-1 1 8 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region_including_nr_lbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1 2.11 A RWork = 0.212 RFree = 0.255 esrra-ppargc1a-1 2 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed ID_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9UBK2:p.Leu144Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L A Wed May 02 16:36:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu144ala' Tue Jun 30 16:08:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu144Ala' Q9UBK2:p.Leu210Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L A Wed May 02 18:49:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu210ala' Tue Jun 30 17:09:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu210Ala' Q9UBK2:p.[Leu144Ala;Leu210Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Wed May 02 17:47:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu144ala,leu210ala' Tue Jun 30 18:03:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu144Ala;Leu210Ala]' box3 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain box2 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey region_including_nr_lbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 2, 4 ppargc1a-esrra-1 2 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed Q9UBK2:p.Leu144Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L A Wed May 02 16:20:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu144ala' Tue Jun 30 17:48:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu144Ala' box2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey region_including_nr_lbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 2-2 ppargc1a-esrra-2 2 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed Q9UBK2:p.Leu211Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L A Wed May 02 18:53:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu211ala' Tue Jun 30 16:22:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu211Ala' Q9UBK2:p.Tyr213Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 17:03:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr213ala' Tue Jun 30 18:55:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr213Ala' Q9UBK2:p.Cys207Ser mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C S Wed May 02 19:36:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys207ser' Tue Jun 30 18:44:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys207Ser' Q9UBK2:p.Cys207Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C E Wed May 02 15:24:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys207glu' Tue Jun 30 19:17:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys207Glu' Q9UBK2:p.Leu210Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L A Wed May 02 15:09:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu210ala' Tue Jun 30 20:10:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu210Ala' box3 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey region_including_nr_lbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 2-3, figure 3A these results revealed that amino acids at positions -6 to -3 (Arg-204/Arg-205 and Cys-207) determine high affinity binding of the PGC-1a box3 region to the ERRa LBD. These results revealed that amino acids at positions -6 to -3 (Arg-204/Arg-205 and Cys-207) determine high affinity binding of the PGC-1a box3 region to the ERRa LBD. ppargc1a-esrra-3 2 4 unspecified role unspecified role prey prey region_including_nr_lbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed Q9UBK2:p.Pro202_Gln203delinsAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain PQ AA Wed May 02 20:33:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro_gln202-203ala_ala' Tue Jun 30 16:48:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro202_Gln203delinsAlaAla' Q9UBK2:p.Arg204_Arg205delinsAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain RR AA Wed May 02 15:23:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg204-205ala_ala' Tue Jun 30 16:14:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg204_Arg205delinsAlaAla' region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain truncated_box3 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association figure 3A-2 These results revealed that amino acids at positions -6 to -3 (Arg-204/Arg-205 and Cys-207) determine high affinity binding of the PGC-1a box3 region to the ERRa LBD. ppargc1a-esrra-4 2 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed ID_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain box3 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey P11474:p.[Met258Val;Gln262Glu] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain M V certain certain sequence position certain certain certain sequence position certain Q E Wed May 02 15:27:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met258val,gln262glu' Tue Jun 30 17:44:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Met258Val;Gln262Glu]' P11474:p.His341Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain H A Wed May 02 14:44:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his341ala' Tue Jun 30 18:59:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His341Ala' P11474:p.Asp423Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A Wed May 02 15:19:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp423ala' Tue Jun 30 16:46:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp423Ala' P11474:p.Met421_Asp423del mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain MMD ... Wed May 02 19:45:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met_met_asp421-423del_del_del' Tue Jun 30 15:44:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met421_Asp423del' region_including_nr_lbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 4A I have not made many separate interactions for the different PGC1a constructs as the results are similar for every one as compared to "ID" construct - apart from maybe D423A mutant- added as an "extra" interaction for "box-2". ppargc1a-esrra-6 2 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed box2 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey P11474:p.[Met258Val;Gln262Glu] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain M V certain certain sequence position certain certain certain sequence position certain Q E Wed May 02 17:17:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met258val,gln262glu' Tue Jun 30 17:33:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Met258Val;Gln262Glu]' P11474:p.His341Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain H A Wed May 02 18:47:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his341ala' Tue Jun 30 20:14:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His341Ala' P11474:p.Asp423Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain D A Wed May 02 19:25:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp423ala' Tue Jun 30 17:53:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp423Ala' P11474:p.Met421_Asp423del mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain MMD ... Wed May 02 20:13:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met_met_asp421-423del_del_del' Tue Jun 30 17:40:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met421_Asp423del' region_including_nr_lbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 4Ai I have not made many separate interactions for the different PGC1a constructs as the results are similar for every one as compared to "ID" construct - apart from maybe D423A mutant- added as an "extra" interaction here. ppargc1a-esrra-5 2 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed box2 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ID_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain box3 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey P11474:p.Asp338_His341delinsGlyValTyrThrPheLeuSerSerThrLeuLysSer mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain DSVH GVYTFLSSTLKS Tue May 01 18:16:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_ser_val_his338-241gly_val_tyr_thr_phe_leu_ser_ser_thr_leu_lys_ser' Wed May 02 19:01:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_ser_val_his338-341gly_val_tyr_thr_phe_leu_ser_ser_thr_leu_lys_ser' Tue Jun 30 19:28:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp338_His341delinsGlyValTyrThrPheLeuSerSerThrLeuLysSer' P11474:p.Asp338Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D A Wed May 02 17:54:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp338ala' Tue Jun 30 17:35:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp338Ala' P11474:p.Arg315Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 19:48:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg315ala' Tue Jun 30 16:19:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg315Ala' region_including_nr_lbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P11474:p.Ser259His mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S H Wed May 02 19:28:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser259his' Tue Jun 30 17:54:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser259His' physical association physical association Figure 4C1 ppargc1a-esrra-7 2 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed Q9UBK2:p.Lys212Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K R Wed May 02 16:44:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys212arg' Tue Jun 30 19:44:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys212Arg' box3 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q9UBK2:p.Leu211Ala mutation mutation certain certain sequence position certain certain certain sequence position certain L A Wed May 02 14:51:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu211ala' Tue Jun 30 15:59:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu211Ala' 4 unspecified role unspecified role prey prey region_including_nr_lbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 3B I have not made many separate interactions for the different PGC1a constructs as the results are similar for every one as compared to "ID" construct - apart from maybe D423A mutant- added as an "extra" interaction for "box-2". ppargc1a-esrra-8 2 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed ID_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey P11474:p.Glu343Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E A Wed May 02 17:28:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu343ala' Tue Jun 30 19:19:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu343Ala' region_including_nr_lbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 4C-2 ppargc1a-esrra-9 2 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed box2 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain box3 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey P11474:p.Glu343Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A Wed May 02 19:42:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu343ala' Tue Jun 30 19:22:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu343Ala' region_including_nr_lbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 4C-3 ppargc1a-esrra-10 2 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed ID_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain box2 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey region_including_nr_lbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P03372:p.[Val376Met;Glu380Gln] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain V M certain certain sequence position certain certain certain sequence position certain E Q Wed May 02 15:23:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val376met,glu380gln' Tue Jun 30 19:51:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val376Met;Glu380Gln]' physical association physical association Figure 5D The wild-type ERa LBD interacted in an estradiol (E2)-dependent manner, detectable only with the PGC-1a ID and box2 but not with box3. E2- estradiol CHEBI:23965 qwertyuiop-1 3 6 unspecified role unspecified role bait bait purified purified region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 4 unspecified role unspecified role prey prey partially purified partially purified P11474:p.Arg315Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 19:08:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg315ala' Tue Jun 30 15:59:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg315Ala' P11474:p.Asp338Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A Wed May 02 15:16:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp338ala' Tue Jun 30 18:20:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp338Ala' P11474:p.Glu343Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A Wed May 02 18:13:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu343ala' Tue Jun 30 17:53:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu343Ala' P11474:p.Asp338_His341delinsGlyValTyrThrPheLeuSerSerThrLeuLysSer mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain DSVH GVYTFLSSTLKS Tue May 01 18:15:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_ser_val_his338-241gly_val_tyr_thr_phe_leu_ser_ser_thr_leu_lys_ser' Wed May 02 16:26:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_ser_val_his338-341gly_val_tyr_thr_phe_leu_ser_ser_thr_leu_lys_ser' Tue Jun 30 16:05:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp338_His341delinsGlyValTyrThrPheLeuSerSerThrLeuLysSer' in vitro In vitro direct interaction direct interaction Figure 6 A/B, supp fig 2B these are entered as mutations decreasing as less dimer is formed due to monomer formation- with DR0 dr0_probe-esrra-1 3 6 competitor competitor competitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role bait bait purified purified region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 4 unspecified role unspecified role prey prey partially purified partially purified in vitro In vitro physical association physical association Figure 6A-2 dr0_probe-esrra-2 3 6 unspecified role unspecified role bait bait purified purified region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 4 unspecified role unspecified role prey prey partially purified partially purified P11474:p.Arg315Ala mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain R A Wed May 02 18:04:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg315ala' Tue Jun 30 19:12:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg315Ala' P11474:p.Asp338Ala mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain D A Wed May 02 19:45:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp338ala' Tue Jun 30 19:48:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp338Ala' P11474:p.Glu343Ala mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain E A Wed May 02 20:30:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu343ala' Tue Jun 30 18:47:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu343Ala' P11474:p.Asp338_His341delinsGlyValTyrThrPheLeuSerSerThrLeuLysSer mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain DSVH GVYTFLSSTLKS Tue May 01 17:43:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_ser_val_his338-241gly_val_tyr_thr_phe_leu_ser_ser_thr_leu_lys_ser' Wed May 02 19:10:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_ser_val_his338-341gly_val_tyr_thr_phe_leu_ser_ser_thr_leu_lys_ser' Tue Jun 30 16:04:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp338_His341delinsGlyValTyrThrPheLeuSerSerThrLeuLysSer' in vitro In vitro direct interaction direct interaction Figure 6 A/B, supp fig 2B Only a weak band is seen here for the DR0-E343A ERRa mutant (monomer). dr0_probe-esrra-3 3 7 unspecified role unspecified role bait bait purified purified region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 4 unspecified role unspecified role prey prey partially purified partially purified P11474:p.Arg315Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R A Wed May 02 19:18:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg315ala' Tue Jun 30 15:44:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg315Ala' P11474:p.Asp338Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain D A Wed May 02 19:42:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp338ala' Tue Jun 30 16:24:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp338Ala' P11474:p.Glu343Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A Wed May 02 17:38:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu343ala' Tue Jun 30 15:40:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu343Ala' P11474:p.Asp338_His341delinsGlyValTyrThrPheLeuSerSerThrLeuLysSer mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain DSVH GVYTFLSSTLKS Tue May 01 17:32:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_ser_val_his338-241gly_val_tyr_thr_phe_leu_ser_ser_thr_leu_lys_ser' Wed May 02 14:40:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_ser_val_his338-341gly_val_tyr_thr_phe_leu_ser_ser_thr_leu_lys_ser' Tue Jun 30 18:51:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp338_His341delinsGlyValTyrThrPheLeuSerSerThrLeuLysSer' in vitro In vitro direct interaction direct interaction Figure 6 A/C these are entered as mutations of NO effect, as all bands look to be at same position and same density - no monomers formed. (text is not clear) ere_probe-esrra-1 3 7 competitor competitor competitor unspecified role unspecified role purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role bait bait purified purified region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 4 unspecified role unspecified role prey prey partially purified partially purified in vitro In vitro direct interaction direct interaction Figure 6A-ii ere_probe-esrra-2 3 8 unspecified role unspecified role prey prey partially purified partially purified ID_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9UBK2:p.[Leu144Ala;Leu210Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Wed May 02 15:12:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu144ala,leu210ala' Tue Jun 30 15:37:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu144Ala;Leu210Ala]' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait purified purified region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 4 unspecified role unspecified role prey prey partially purified partially purified P11474:p.Arg315Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 15:48:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg315ala' Tue Jun 30 19:34:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg315Ala' P11474:p.Asp338Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A Wed May 02 20:23:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp338ala' Tue Jun 30 19:20:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp338Ala' P11474:p.Glu343Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E A Wed May 02 16:53:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu343ala' Tue Jun 30 17:47:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu343Ala' P11474:p.Asp338_His341delinsGlyValTyrThrPheLeuSerSerThrLeuLysSer mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain DSVH GVYTFLSSTLKS Tue May 01 17:43:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_ser_val_his338-241gly_val_tyr_thr_phe_leu_ser_ser_thr_leu_lys_ser' Wed May 02 15:28:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_ser_val_his338-341gly_val_tyr_thr_phe_leu_ser_ser_thr_leu_lys_ser' Tue Jun 30 18:06:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp338_His341delinsGlyValTyrThrPheLeuSerSerThrLeuLysSer' in vitro In vitro physical association physical association Figure 6B-1, supp fig 2B The addition of small amounts of E. coli-expressed, partially purified GST-PGC-1a ID fusion protein resulted in an upshift of DNA-bound ERRa (Fig. 6, B and C). This upshift was not observed when GST-PGC-1a ID(L144A/L210A) was added. Compared with the wild-type receptor, all DNA-bound mutants were less efficiently shifted by GST-PGC-1a ID. Pablo agrees that the shift with GST-PGC1a shows binding, so that LESS of shift shows a decrease in binding. (hard to see!) Mutations in the H8–H9 loop such as R315A or D338A influence not only homodimerization but also Coactivator binding of DNA-bound, full-length ERRa. dr0_probe-esrra-4 3 8 inhibitor inhibitor prey prey partially purified partially purified ID_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait purified purified region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 4 unspecified role unspecified role prey prey partially purified partially purified P11474:p.Arg315Ala mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain R A Wed May 02 15:40:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg315ala' Tue Jun 30 15:59:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg315Ala' P11474:p.Asp338Ala mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain D A Wed May 02 20:25:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp338ala' Tue Jun 30 19:51:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp338Ala' P11474:p.Glu343Ala mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain E A Wed May 02 15:35:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu343ala' Tue Jun 30 17:13:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu343Ala' in vitro In vitro direct interaction direct interaction Figure 6B, supp fig 2B Only a very weak band is seen here for the DR0-E343A ERRa mutant (monomer). DNA-bound ERRa(SwapH8–H9) did not interact with GST-PGC-1a ID. ere_probe-esrra-3 3 8 unspecified role unspecified role prey prey partially purified partially purified ID_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9UBK2:p.[Leu144Ala;Leu210Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Wed May 02 20:05:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu144ala,leu210ala' Tue Jun 30 20:23:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu144Ala;Leu210Ala]' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait purified purified region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 4 unspecified role unspecified role prey prey partially purified partially purified P11474:p.Arg315Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 18:15:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg315ala' Tue Jun 30 17:09:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg315Ala' P11474:p.Asp338Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A Wed May 02 14:38:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp338ala' Tue Jun 30 16:54:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp338Ala' P11474:p.Glu343Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E A Wed May 02 19:02:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu343ala' Tue Jun 30 19:23:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu343Ala' P11474:p.Asp338_His341delinsGlyValTyrThrPheLeuSerSerThrLeuLysSer mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain DSVH GVYTFLSSTLKS Tue May 01 18:14:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_ser_val_his338-241gly_val_tyr_thr_phe_leu_ser_ser_thr_leu_lys_ser' Wed May 02 19:16:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp_ser_val_his338-341gly_val_tyr_thr_phe_leu_ser_ser_thr_leu_lys_ser' Tue Jun 30 16:49:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp338_His341delinsGlyValTyrThrPheLeuSerSerThrLeuLysSer' in vitro In vitro physical association physical association Figure 6C The addition of small amounts of E. coli-expressed, partially purified GST-PGC-1a ID fusion protein resulted in an upshift of DNA-bound ERRa (Fig. 6, B and C). This upshift was not observed when GST-PGC-1a ID(L144A/L210A) was added. Compared with the wild-type receptor, all DNA-bound mutants were less efficiently shifted by GST-PGC-1a ID. Pablo agrees that the shift with GST-PGC1a shows binding, so that LESS of shift shows a decrease in binding. (can be hard to see!) Mutations in the H8–H9 loop such as R315A or D338A influence not only homodimerization but also Coactivator binding of DNA-bound, full-length ERRa.