IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The human TPR protein TTC4 is a putative Hsp90 co-chaperone which interacts with CDC6 and shows alterations in transformed cells. The human TPR protein TTC4 is a putative Hsp90 co-chaperone which interacts with CDC6 and shows alterations in transformed cells. PloS one 2007 imex curation Crevel G., Bennett D., Cotterill S. s.cotterill@sghms.ac.uk 17-May-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast lexa b52 complement lexa b52 complementation LexA B52 transcription complementation lexa b52 complement predetermined predetermined participant predetermined Crevel G., Bennett D., Cotterill S. s.cotterill@sghms.ac.uk Accepted 2018-MAY-10 AT 08:51 BST AT 08:51 BST by LIVIAP PloS one 2007 Only protein-protein interactions imex curation imex curation I have altered all mutations in EBI-20230709 so the 'mutations of no effect' are now all 'mutations decreasing' - as all are only 65% -70% of max. EBI-20230705 altered so mutations that are 75% or less of Max are now "mutations decreasing". The comment is not really relevant - I only add comments to the effect "the authors have stated xyz...." when I do not believe them, or results are unclear. EBI-20230722 - deleted as this interaction is not missing from anywhere, and I dont know why it is there. The human TPR protein TTC4 is a putative Hsp90 co-chaperone which interacts with CDC6 and shows alterations in transformed cells. The human TPR protein TTC4 is a putative Hsp90 co-chaperone which interacts with CDC6 and shows alterations in transformed cells. PloS one 2007 imex curation Crevel G., Bennett D., Cotterill S. s.cotterill@sghms.ac.uk 17-May-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Crevel G., Bennett D., Cotterill S. I can find NO expression system/vectors for the purified proteins s.cotterill@sghms.ac.uk Accepted 2018-MAY-10 AT 08:51 BST AT 08:51 BST by LIVIAP PloS one 2007 Only protein-protein interactions imex curation imex curation I have altered all mutations in EBI-20230709 so the 'mutations of no effect' are now all 'mutations decreasing' - as all are only 65% -70% of max. EBI-20230705 altered so mutations that are 75% or less of Max are now "mutations decreasing". The comment is not really relevant - I only add comments to the effect "the authors have stated xyz...." when I do not believe them, or results are unclear. EBI-20230722 - deleted as this interaction is not missing from anywhere, and I dont know why it is there. The human TPR protein TTC4 is a putative Hsp90 co-chaperone which interacts with CDC6 and shows alterations in transformed cells. The human TPR protein TTC4 is a putative Hsp90 co-chaperone which interacts with CDC6 and shows alterations in transformed cells. PloS one 2007 imex curation Crevel G., Bennett D., Cotterill S. s.cotterill@sghms.ac.uk 17-May-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Crevel G., Bennett D., Cotterill S. s.cotterill@sghms.ac.uk Accepted 2018-MAY-10 AT 08:51 BST AT 08:51 BST by LIVIAP PloS one 2007 Only protein-protein interactions imex curation imex curation I have altered all mutations in EBI-20230709 so the 'mutations of no effect' are now all 'mutations decreasing' - as all are only 65% -70% of max. EBI-20230705 altered so mutations that are 75% or less of Max are now "mutations decreasing". The comment is not really relevant - I only add comments to the effect "the authors have stated xyz...." when I do not believe them, or results are unclear. EBI-20230722 - deleted as this interaction is not missing from anywhere, and I dont know why it is there. hsp7c_human Heat shock cognate 71 kDa protein HSPA8 HSC70 HSP73 HSPA10 Heat shock 70 kDa protein 8 Lipopolysaccharide-associated protein 1 protein protein human Homo sapiens Human MSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLLLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIINWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD 9AA27B210730670C hs90b_human Heat shock protein HSP 90-beta HSP90AB1 HSP90B HSPC2 HSPCB Heat shock 84 kDa protein protein human Homo sapiens Human MPEEVHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDALDKIRYESLTDPSKLDSGKELKIDIIPNPQERTLTLVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVVVITKHNDDEQYAWESSAGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKEVVKKHSQFIGYPITLYLEKEREKEISDDEAEEEKGEKEEEDKDDEEKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWTRNPDDITQEEYGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFIPRRAPFDLFENKKKKNNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRRLSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKAVKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVAAEEPNAAVPDEIPPLEGDEDASRMEEVD DIP protein P08238 original sequence version: 91 DIP protein P08238 original sequence version: 149 DIP protein P08238 original sequence version: 165 DIP protein P08238 original sequence version: 167 DIP protein P08238 original sequence version: 210 DIP protein P08238 original sequence version: 187 A93118C214D03810 cdc6_human Cell division control protein 6 homolog CDC6 CDC18L CDC6-related protein p62(cdc6) Cdc18-related protein protein protein human Homo sapiens Human MPQTRSQAQATISFPKRKLSRALNKAKNSSDAKLEPTNVQTVTCSPRVKALPLSPRKRLGDDNLCNTPHLPPCSPPKQGKKENGPPHSHTLKGRRLVFDNQLTIKSPSKRELAKVHQNKILSSVRKSQEITTNSEQRCPLKKESACVRLFKQEGTCYQQAKLVLNTAVPDRLPAREREMDVIRNFLREHICGKKAGSLYLSGAPGTGKTACLSRILQDLKKELKGFKTIMLNCMSLRTAQAVFPAIAQEICQEEVSRPAGKDMMRKLEKHMTAEKGPMIVLVLDEMDQLDSKGQDVLYTLFEWPWLSNSHLVLIGIANTLDLTDRILPRLQAREKCKPQLLNFPPYTRNQIVTILQDRLNQVSRDQVLDNAAVQFCARKVSAVSGDVRKALDVCRRAIEIVESDVKSQTILKPLSECKSPSEPLIPKRVGLIHISQVISEVDGNRMTLSQEGAQDSFPLQQKILVCSLMLLIRQLKIKEVTLGKLYEAYSKVCRKQQVAAVDQSECLSLSGLLEARGILGLKRNKETRLTKVFFKIEEKEIEHALKDKALIGNILATGLP DIP protein Q99741 original sequence version: 102 3ED7DE4AF80CB017 ttc4_human Tetratricopeptide repeat protein 4 TTC4 My044 protein protein human Homo sapiens Human MEQPGQDPTSDDVMDSFLEKFQSQPYRGGFHEDQWEKEFEKVPLFMSRAPSEIDPRENPDLACLQSIIFDEERSPEEQAKTYKDEGNDYFKEKDYKKAVISYTEGLKKKCADPDLNAVLYTNRAAAQYYLGNFRSALNDVTAARKLKPCHLKAIIRGALCHLELKHFAEAVNWCDEGLQIDAKEKKLLEMRAKADKLKRIEQRDVRKANLKEKKERNQNEALLQAIKARNIRLSEAACEDEDSASEGLGELFLDGLSTENPHGARLSLDGQGRLSWPVLFLYPEYAQSDFISAFHEDSRFIDHLMVMFGETPSWDLEQKYCPDNLEVYFEDEDRAELYRVPAKSTLLQVLQHQRYFVKALTPAFLVCVGSSPFCKNFLRGRKVYQIR 4FA45889C4C9E427 qwer467-1 1 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed O95801:p.Ile67Met mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain I M Tue May 01 18:05:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'le67met' Wed May 02 18:23:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile67met' Tue Jun 30 17:32:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile67Met' O95801:p.Glu77Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain E A Wed May 02 15:45:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu77ala' Tue Jun 30 19:54:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu77Ala' O95801:p.Asn217Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N K Wed May 02 15:09:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn217lys' Tue Jun 30 19:44:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn217Lys' O95801:p.Val42Asp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V D Wed May 02 20:32:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val42asp' Tue Jun 30 18:18:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val42Asp' physical association physical association Figure 2, Table 1 hsp90ab1-ttc4-1 1 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell O95801:p.Val42Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain V D Wed May 02 18:30:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val42asp' Tue Jun 30 17:47:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val42Asp' O95801:p.Ile67Met mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I M Wed May 02 19:07:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile67met' Tue Jun 30 20:03:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile67Met' O95801:p.Glu77Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A Wed May 02 15:09:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu77ala' Tue Jun 30 17:14:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu77Ala' O95801:p.Asn217Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N K Wed May 02 20:06:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn217lys' Tue Jun 30 18:18:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn217Lys' physical association physical association Figure 2, Table 1 It is stated that "None of these mutations had any significant effect on the interactions of TTC4 with the HSP90 and 70 proteins." 678yuoy-1 2 5 unspecified role unspecified role prey prey purified purified 7 unspecified role unspecified role bait bait purified purified O95801:p.Lys152Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K E Wed May 02 15:30:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys152glu' Tue Jun 30 18:23:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys152Glu' O95801:p.Arg156Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R E Wed May 02 18:20:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg156glu' Tue Jun 30 17:48:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg156Glu' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O95801:p.[Lys152Glu;Arg156Glu] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain R E Wed May 02 17:25:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys152glu,arg156glu' Tue Jun 30 17:35:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys152Glu;Arg156Glu]' direct interaction direct interaction Figure 4 hspa8-ttc4-2 1 6 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed O95801:p.Val42Asp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V D Wed May 02 17:34:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val42asp' Tue Jun 30 17:47:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val42Asp' O95801:p.Ile67Met mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain I M Wed May 02 16:25:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile67met' Tue Jun 30 18:19:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile67Met' O95801:p.Glu77Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain E A Wed May 02 14:39:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu77ala' Tue Jun 30 20:09:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu77Ala' O95801:p.Asn217Lys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain N K Wed May 02 16:22:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn217lys' Tue Jun 30 18:09:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn217Lys' physical association physical association Figure 5, Table 1 It is stated that "None of these mutations had any significant effect on the interactions of TTC4 with the HSP90 and 70 proteins." 15t4rwatg\-1 3 7 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 6 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 4 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level association association Figure 3B