IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ KIR2DS5 allotypes that recognize the C2 epitope of HLA-C are common among Africans and absent from Europeans. KIR2DS5 allotypes that recognize the C2 epitope of HLA-C are common among Africans and absent from Europeans. Immunity, inflammation and disease 2017 imex curation test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test, Test, test, test2, Blokhuis JH., Hilton HG., Guethlein LA., Norman PJ., Nemat-Gorgani N., Nakimuli A., Chazara O., Moffett A., Parham P., Test, Test UniProt Updates informed (reply 20/10/17 acknowledging reciept) that KIR2DS5*001 sequence which correponds to Q14953 is nbg - and requested variants be listed. lisbeth.guethlein@stanford.edu 27-Mar-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro affinity techniques affinity technology affinity techniques antibody detection identification by antibody antibody detection test Alleles KIR2DS5*002, KIR2DS5*009, KIR2DS5*010 and KIR2DS5*011 have activating potential but do not recocognize (or with very slight avidity) C2 epitopes of HLA-C alleles. UniProt Updates informed (reply 20/10/17 acknowledging reciept) that KIR2DS5*001 sequence which correponds to Q14953 is nbg - and requested variants be listed. Assays using Luminex® bead-based multiplexing technology:color-coded microbeads coated with purified HLA antigens and tested for binding to KIR-Fc fusion proteins. A secondary PE conjugated antibody is added that binds to the Fc portion of the KIR–Fc. Binding was detected by fluorescence - either flow cytometry or fluorescence microscopy. Results > 0.04 relative to W632 antibody have been entered as positive results. A KIR2DS5*005 allele construct was used as a template for all other alleles. The sequence is stated to be "residues 1–224 of the mature protein";this has been assumed to be aas 22-245 - the ECD. Uniprot entry Q14953 (KIR2DS5) gives the sequence for allele KIR2DS5*002;all other alleles have been entered as variants/mutations of this sequence. lisbeth.guethlein@stanford.edu Accepted 2018-MAR-15 AT 08:33 GMT AT 08:33 GMT by LIVIAP Immunity, inflammation and disease 2017 Only protein-protein interactions imex curation imex curation The source is "human" - as "HLA" stands for Human Leucocyte Antigens - no other species with this name in UniProt! The HLA-C molecules are from the supplier of Luminex coated Beads -LabScreen Single-Antigen beads lot #8, One Lambda. They do not say how/where/why they obtain the HLA molecules, other than that they are "purified". Several other of this type of experiments are already in Intact, so it is OK to add these interactions I think. hlac_human HLA class I histocompatibility antigen, C alpha chain HLA-Cw Human leukocyte antigen C HLAC HLA-C protein protein human Homo sapiens Human MRVMAPRALLLLLSGGLALTETWACSHSMRYFDTAVSRPGRGEPRFISVGYVDDTQFVRFDSDAASPRGEPRAPWVEQEGPEYWDRETQKYKRQAQADRVSLRNLRGYYNQSEDGSHTLQRMSGCDLGPDGRLLRGYDQSAYDGKDYIALNEDLRSWTAADTAAQITQRKLEAARAAEQLRAYLEGTCVEWLRRYLENGKETLQRAEPPKTHVTHHPLSDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGQEQRYTCHMQHEGLQEPLTLSWEPSSQPTIPIMGIVAGLAVLVVLAVLGAVVTAMMCRRKSSGGKGGSCSQAACSNSAQGSDESLITCKA DIP protein P30504 original sequence version: 126 59C23D95FD1D0BC8 1c02_human HLA class I histocompatibility antigen, Cw-2 alpha chain HLA-C HLAC MHC class I antigen Cw*2 protein protein human Homo sapiens Human MRVMEPRTLILLLSGALALTETWACSHSMRYFYTAVSRPSRGEPHFIAVGYVDDTQFVRFDSDAASPRGEPRGRWVEQEGPEYWDRETQKYNRQAQTDRVNLRKLRGYYNQSEAGSHTLQRMYGCDLGPDGRLLRGYDQSAYDGKDYIALNEDLRSWTAADTAAQITQRKWEAAREAEEWRAYLEGECVEWLRRYLENGKEKLQRAEHPKTHVTHHPVSDHEATLRCWALGFYPTEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPEPLTLRWEPSSQPTIPIVGIVAGLAVLAVLAVLGAVVAVVMCRRKSSGGKGGSCSQAASSNSAQGSDESLIASKA The protein could not be merged with EBI-1051396 because of some conflicts with the protein sequence (features which cannot be shifted). AD8025DEB7DA8CE6 ki2s5_human Killer cell immunoglobulin-like receptor 2DS5 KIR2DS5 CD158G NKAT9 CD158 antigen-like family member G MHC class I NK cell receptor Natural killer-associated transcript 9 protein protein human Homo sapiens Human MSLMVISMACVAFFLLQGAWPHEGFRRKPSLLAHPGPLVKSEETVILQCWSDVMFEHFLLHREGTFNHTLRLIGEHIDGVSKGNFSIGRMTQDLAGTYRCYGSVTHSPYQLSAPSDPLDIVITGLYEKPSLSAQPGPTVLAGESVTLSCSSRSSYDMYHLSREGEAHERRLPAGPKVNRTFQADFPLDPATHGGTYRCFGSFRDSPYEWSKSSDPLLVSVTGNSSNSWPSPTEPSSETGNPRHLHVLIGTSVVKLPFTILLFFLLHRWCSNKKNASVMDQGPAGNRTVNREDSDEQDHQEVSYA 83AFBB6A08D8DC9B hla_c-kir2ds5-1 1 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ser144asn variant variant certain certain sequence position certain certain certain sequence position certain S N arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 3 unspecified role unspecified role bait bait purified purified glu179gln variant variant certain certain sequence position certain certain certain sequence position certain E Q lys202thr variant variant certain certain sequence position certain certain certain sequence position certain K T ser364cys variant variant certain certain sequence position certain certain certain sequence position certain S C ile10leu variant variant certain certain sequence position certain certain certain sequence position certain I L gly_arg_73-74ala_pro variant variant certain certain sequence position certain certain certain sequence position certain GR AP glu5ala variant variant certain certain sequence position certain certain certain sequence position certain E A asn92lys variant variant certain certain sequence position certain certain certain sequence position certain N K direct interaction direct interaction Supplementary figure 1A-1 C*02:02 allele, KIR2DS5*003 allele hla_c-kir2ds5-2 1 3 unspecified role unspecified role bait bait purified purified glu179gln variant variant certain certain sequence position certain certain certain sequence position certain E Q lys202thr variant variant certain certain sequence position certain certain certain sequence position certain K T ser364cys variant variant certain certain sequence position certain certain certain sequence position certain S C ile10leu variant variant certain certain sequence position certain certain certain sequence position certain I L gly_arg_73-74ala_pro variant variant certain certain sequence position certain certain certain sequence position certain GR AP glu5ala variant variant certain certain sequence position certain certain certain sequence position certain E A asn92lys variant variant certain certain sequence position certain certain certain sequence position certain N K 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg203his variant variant certain certain sequence position certain certain certain sequence position certain R H arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supplementary figure 1A-2 C*02:02 allele, KIR2DS5*004 allele hla_c-kir2ds5-3 1 3 unspecified role unspecified role bait bait purified purified glu179gln variant variant certain certain sequence position certain certain certain sequence position certain E Q lys202thr variant variant certain certain sequence position certain certain certain sequence position certain K T ser364cys variant variant certain certain sequence position certain certain certain sequence position certain S C ile10leu variant variant certain certain sequence position certain certain certain sequence position certain I L gly_arg_73-74ala_pro variant variant certain certain sequence position certain certain certain sequence position certain GR AP glu5ala variant variant certain certain sequence position certain certain certain sequence position certain E A asn92lys variant variant certain certain sequence position certain certain certain sequence position certain N K 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supplementary figure 1A-3 C*02:02 allele, KIR2DS5*005 allele hla_c-kir2ds5-4 1 3 unspecified role unspecified role bait bait purified purified glu179gln variant variant certain certain sequence position certain certain certain sequence position certain E Q lys202thr variant variant certain certain sequence position certain certain certain sequence position certain K T ser364cys variant variant certain certain sequence position certain certain certain sequence position certain S C ile10leu variant variant certain certain sequence position certain certain certain sequence position certain I L gly_arg_73-74ala_pro variant variant certain certain sequence position certain certain certain sequence position certain GR AP glu5ala variant variant certain certain sequence position certain certain certain sequence position certain E A asn92lys variant variant certain certain sequence position certain certain certain sequence position certain N K 4 unspecified role unspecified role prey prey purified purified pro175thr variant variant certain certain sequence position certain certain certain sequence position certain P T ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supplementary figure 1A-4 C*02:02 allele, KIR2DS5*006 allele hla_c-kir2ds5-5 1 3 unspecified role unspecified role bait bait purified purified glu179gln variant variant certain certain sequence position certain certain certain sequence position certain E Q lys202thr variant variant certain certain sequence position certain certain certain sequence position certain K T ser364cys variant variant certain certain sequence position certain certain certain sequence position certain S C ile10leu variant variant certain certain sequence position certain certain certain sequence position certain I L gly_arg_73-74ala_pro variant variant certain certain sequence position certain certain certain sequence position certain GR AP glu5ala variant variant certain certain sequence position certain certain certain sequence position certain E A asn92lys variant variant certain certain sequence position certain certain certain sequence position certain N K 4 unspecified role unspecified role prey prey purified purified glu237lys variant variant certain certain sequence position certain certain certain sequence position certain E K ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supplementary figure 1A-5 C*02:02 allele, KIR2DS5*007 allele hla_c-kir2ds5-6 1 3 unspecified role unspecified role bait bait purified purified glu179gln variant variant certain certain sequence position certain certain certain sequence position certain E Q lys202thr variant variant certain certain sequence position certain certain certain sequence position certain K T ser364cys variant variant certain certain sequence position certain certain certain sequence position certain S C ile10leu variant variant certain certain sequence position certain certain certain sequence position certain I L gly_arg_73-74ala_pro variant variant certain certain sequence position certain certain certain sequence position certain GR AP glu5ala variant variant certain certain sequence position certain certain certain sequence position certain E A asn92lys variant variant certain certain sequence position certain certain certain sequence position certain N K 4 unspecified role unspecified role prey prey purified purified his22arg variant variant certain certain sequence position certain certain certain sequence position certain H R ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supplementary figure 1A-6 C*02:02 allele, KIR2DS5*008 allele hla_c-kir2ds5-7 1 2 unspecified role unspecified role bait bait purified purified 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ser144asn variant variant certain certain sequence position certain certain certain sequence position certain S N arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supplementary figure 1A-1a C*05:01 allele, KIR2DS5*003 allele hla_c-kir2ds5-8 1 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg203his variant variant certain certain sequence position certain certain certain sequence position certain R H arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 2 unspecified role unspecified role bait bait purified purified direct interaction direct interaction Supplementary figure 1A-2a C*05:01 allele, KIR2DS5*004 allele hla_c-kir2ds5-9 1 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 2 unspecified role unspecified role bait bait purified purified direct interaction direct interaction Supplementary figure 1A-3a C*05:01 allele, KIR2DS5*005 allele hla_c-kir2ds5-10 1 2 unspecified role unspecified role bait bait purified purified 4 unspecified role unspecified role prey prey purified purified pro175thr variant variant certain certain sequence position certain certain certain sequence position certain P T ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supplementary figure 1A-4a C*05:01 allele, KIR2DS5*006 allele hla_c-kir2ds5-11 1 2 unspecified role unspecified role bait bait purified purified 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G glu237lys variant variant certain certain sequence position certain certain certain sequence position certain E K trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supplementary figure 1A-5a C*05:01 allele, KIR2DS5*007 allele hla_c-kir2ds5-12 1 2 unspecified role unspecified role bait bait purified purified 4 unspecified role unspecified role prey prey purified purified his22arg variant variant certain certain sequence position certain certain certain sequence position certain H R ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supplementary figure 1A-6a C*05:01 allele, KIR2DS5*008 allele hla_c-kir2ds5-13 1 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ser144asn variant variant certain certain sequence position certain certain certain sequence position certain S N arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 2 unspecified role unspecified role bait bait purified purified direct interaction direct interaction Supplementary figure 1A-1b C*06:02 allele, KIR2DS5*003 allele hla_c-kir2ds5-14 1 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg203his variant variant certain certain sequence position certain certain certain sequence position certain R H arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 2 unspecified role unspecified role bait bait purified purified direct interaction direct interaction Supplementary figure 1A-2b C*06:02 allele, KIR2DS5*004 allele hla_c-kir2ds5-15 1 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 2 unspecified role unspecified role bait bait purified purified direct interaction direct interaction Supplementary figure 1A-3b C*06:02 allele, KIR2DS5*005 allele hla_c-kir2ds5-16 1 2 unspecified role unspecified role bait bait purified purified 4 unspecified role unspecified role prey prey purified purified pro175thr variant variant certain certain sequence position certain certain certain sequence position certain P T ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supplementary figure 1A-4b C*06:02 allele, KIR2DS5*006 allele hla_c-kir2ds5-17 1 2 unspecified role unspecified role bait bait purified purified 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G glu237lys variant variant certain certain sequence position certain certain certain sequence position certain E K trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supplementary figure 1A-5b C*06:02 allele, KIR2DS5*007 allele hla_c-kir2ds5-18 1 2 unspecified role unspecified role bait bait purified purified 4 unspecified role unspecified role prey prey purified purified his22arg variant variant certain certain sequence position certain certain certain sequence position certain H R ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supplementary figure 1A-6b C*06:02 allele, KIR2DS5*008 allele hla_c-kir2ds5-19 1 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ser144asn variant variant certain certain sequence position certain certain certain sequence position certain S N arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 2 unspecified role unspecified role bait bait purified purified direct interaction direct interaction Supplementary figure 1A-1c C*15:02 allele, KIR2DS5*003 allele hla_c-kir2ds5-20 1 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg203his variant variant certain certain sequence position certain certain certain sequence position certain R H arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 2 unspecified role unspecified role bait bait purified purified direct interaction direct interaction Supplementary figure 1A-2c C*15:02 allele, KIR2DS5*004 allele hla_c-kir2ds5-21 1 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 2 unspecified role unspecified role bait bait purified purified direct interaction direct interaction Supplementary figure 1A-3c C*15:02 allele, KIR2DS5*005 allele hla_c-kir2ds5-22 1 2 unspecified role unspecified role bait bait purified purified 4 unspecified role unspecified role prey prey purified purified pro175thr variant variant certain certain sequence position certain certain certain sequence position certain P T ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supplementary figure 1A-4c C*15:02 allele, KIR2DS5*006 allele hla_c-kir2ds5-23 1 2 unspecified role unspecified role bait bait purified purified 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G glu237lys variant variant certain certain sequence position certain certain certain sequence position certain E K trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supplementary figure 1A-5c C*15:02 allele, KIR2DS5*007 allele hla_c-kir2ds5-24 1 2 unspecified role unspecified role bait bait purified purified 4 unspecified role unspecified role prey prey purified purified his22arg variant variant certain certain sequence position certain certain certain sequence position certain H R ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supplementary figure 1A-6c C*15:02 allele, KIR2DS5*008 allele hla_c-kir2ds5-25 1 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ser144asn variant variant certain certain sequence position certain certain certain sequence position certain S N arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 2 unspecified role unspecified role bait bait purified purified direct interaction direct interaction Supplementary figure 1A-1d C*17:01 allele, KIR2DS5*003 allele hla_c-kir2ds5-26 1 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg203his variant variant certain certain sequence position certain certain certain sequence position certain R H arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 2 unspecified role unspecified role bait bait purified purified direct interaction direct interaction Supplementary figure 1A-2d C*17:01 allele, KIR2DS5*004 allele hla_c-kir2ds5-27 1 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 2 unspecified role unspecified role bait bait purified purified direct interaction direct interaction Supplementary figure 1A-3d C*17:01 allele, KIR2DS5*005 allele hla_c-kir2ds5-28 1 2 unspecified role unspecified role bait bait purified purified 4 unspecified role unspecified role prey prey purified purified pro175thr variant variant certain certain sequence position certain certain certain sequence position certain P T ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supplementary figure 1A-4d C*17:01 allele, KIR2DS5*006 allele hla_c-kir2ds5-29 1 2 unspecified role unspecified role bait bait purified purified 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G glu237lys variant variant certain certain sequence position certain certain certain sequence position certain E K trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supplementary figure 1A-5d C*17:01 allele, KIR2DS5*007 allele hla_c-kir2ds5-30 1 2 unspecified role unspecified role bait bait purified purified 4 unspecified role unspecified role prey prey purified purified his22arg variant variant certain certain sequence position certain certain certain sequence position certain H R ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supplementary figure 1A-6d C*17:01 allele, KIR2DS5*008 allele hla_c-kir2ds5-31 1 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ser144asn variant variant certain certain sequence position certain certain certain sequence position certain S N arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 2 unspecified role unspecified role bait bait purified purified val319ala variant variant certain certain sequence position certain certain certain sequence position certain V A direct interaction direct interaction Supplementary figure 1A-1e C*18:02 allele, KIR2DS5*003 allele hla_c-kir2ds5-32 1 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg203his variant variant certain certain sequence position certain certain certain sequence position certain R H arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 2 unspecified role unspecified role bait bait purified purified val319ala variant variant certain certain sequence position certain certain certain sequence position certain V A direct interaction direct interaction Supplementary figure 1A-2e C*18:02 allele, KIR2DS5*004 allele hla_c-kir2ds5-33 1 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 2 unspecified role unspecified role bait bait purified purified val319ala variant variant certain certain sequence position certain certain certain sequence position certain V A direct interaction direct interaction Supplementary figure 1A-3e C*18:02 allele, KIR2DS5*005 allele hla_c-kir2ds5-34 1 2 unspecified role unspecified role bait bait purified purified val319ala variant variant certain certain sequence position certain certain certain sequence position certain V A 4 unspecified role unspecified role prey prey purified purified pro175thr variant variant certain certain sequence position certain certain certain sequence position certain P T ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supplementary figure 1A-4e C*18:02 allele, KIR2DS5*006 allele hla_c-kir2ds5-35 1 2 unspecified role unspecified role bait bait purified purified val319ala variant variant certain certain sequence position certain certain certain sequence position certain V A 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G glu237lys variant variant certain certain sequence position certain certain certain sequence position certain E K trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supplementary figure 1A-5e C*18:02 allele, KIR2DS5*007 allele hla_c-kir2ds5-36 1 2 unspecified role unspecified role bait bait purified purified val319ala variant variant certain certain sequence position certain certain certain sequence position certain V A 4 unspecified role unspecified role prey prey purified purified his22arg variant variant certain certain sequence position certain certain certain sequence position certain H R ecd sufficient to bind sufficient binding region sufficient to bind range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arg179gly variant variant certain certain sequence position certain certain certain sequence position certain R G trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Supplementary figure 1A-6e C*18:02 allele, KIR2DS5*008 allele