IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Polyglutamine tracts regulate beclin 1-dependent autophagy. Polyglutamine tracts regulate beclin 1-dependent autophagy. Nature 2017 imex curation Ashkenazi A., Bento CF., Ricketts T., Vicinanza M., Siddiqi F., Pavel M., Squitieri F., Hardenberg MC., Imarisio S., Menzies FM., Rubinsztein DC. pcDNA–Flag–beclin 1 is C-terminally FLAG tagged - Ref 30 supplement (pFLAG-CMV™-5a -SIGMA) The authors have stated that HA-AR and eGFP-ATN1 (as used in Extended Data figure 10) are of Human origin. dcr1000@cam.ac.uk 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro deubiquitinase assay deubiquitinase assay deubiquitinase assay anti tag western anti tag western blot anti tag western Ashkenazi A., Bento CF., Ricketts T., Vicinanza M., Siddiqi F., Pavel M., Squitieri F., Hardenberg MC., Imarisio S., Menzies FM., Rubinsztein DC. pcDNA–Flag–beclin 1 is C-terminally FLAG tagged - Ref 30 supplement (pFLAG-CMV™-5a -SIGMA) The authors have stated that HA-AR and eGFP-ATN1 (as used in Extended Data figure 10) are of Human origin. dcr1000@cam.ac.uk Accepted 2017-AUG-08 AT 09:41 BST AT 09:41 BST by ORCHARD Nature 2017 imex curation altered both mutations to "decreasing rate" Polyglutamine tracts regulate beclin 1-dependent autophagy. Polyglutamine tracts regulate beclin 1-dependent autophagy. Nature 2017 imex curation Ashkenazi A., Bento CF., Ricketts T., Vicinanza M., Siddiqi F., Pavel M., Squitieri F., Hardenberg MC., Imarisio S., Menzies FM., Rubinsztein DC. pcDNA–Flag–beclin 1 is C-terminally FLAG tagged - Ref 30 supplement (pFLAG-CMV™-5a -SIGMA) The authors have stated that HA-AR and eGFP-ATN1 (as used in Extended Data figure 10) are of Human origin. dcr1000@cam.ac.uk 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Ashkenazi A., Bento CF., Ricketts T., Vicinanza M., Siddiqi F., Pavel M., Squitieri F., Hardenberg MC., Imarisio S., Menzies FM., Rubinsztein DC. pcDNA–Flag–beclin 1 is C-terminally FLAG tagged - Ref 30 supplement (pFLAG-CMV™-5a -SIGMA) The authors have stated that HA-AR and eGFP-ATN1 (as used in Extended Data figure 10) are of Human origin. dcr1000@cam.ac.uk Accepted 2017-AUG-08 AT 09:41 BST AT 09:41 BST by ORCHARD Nature 2017 imex curation altered both mutations to "decreasing rate" Polyglutamine tracts regulate beclin 1-dependent autophagy. Polyglutamine tracts regulate beclin 1-dependent autophagy. Nature 2017 imex curation Ashkenazi A., Bento CF., Ricketts T., Vicinanza M., Siddiqi F., Pavel M., Squitieri F., Hardenberg MC., Imarisio S., Menzies FM., Rubinsztein DC. pcDNA–Flag–beclin 1 is C-terminally FLAG tagged - Ref 30 supplement (pFLAG-CMV™-5a -SIGMA) The authors have stated that HA-AR and eGFP-ATN1 (as used in Extended Data figure 10) are of Human origin. dcr1000@cam.ac.uk 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Ashkenazi A., Bento CF., Ricketts T., Vicinanza M., Siddiqi F., Pavel M., Squitieri F., Hardenberg MC., Imarisio S., Menzies FM., Rubinsztein DC. pcDNA–Flag–beclin 1 is C-terminally FLAG tagged - Ref 30 supplement (pFLAG-CMV™-5a -SIGMA) The authors have stated that HA-AR and eGFP-ATN1 (as used in Extended Data figure 10) are of Human origin. dcr1000@cam.ac.uk Accepted 2017-AUG-08 AT 09:41 BST AT 09:41 BST by ORCHARD Nature 2017 imex curation altered both mutations to "decreasing rate" Polyglutamine tracts regulate beclin 1-dependent autophagy. Polyglutamine tracts regulate beclin 1-dependent autophagy. Nature 2017 imex curation Ashkenazi A., Bento CF., Ricketts T., Vicinanza M., Siddiqi F., Pavel M., Squitieri F., Hardenberg MC., Imarisio S., Menzies FM., Rubinsztein DC. pcDNA–Flag–beclin 1 is C-terminally FLAG tagged - Ref 30 supplement (pFLAG-CMV™-5a -SIGMA) The authors have stated that HA-AR and eGFP-ATN1 (as used in Extended Data figure 10) are of Human origin. dcr1000@cam.ac.uk 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ashkenazi A., Bento CF., Ricketts T., Vicinanza M., Siddiqi F., Pavel M., Squitieri F., Hardenberg MC., Imarisio S., Menzies FM., Rubinsztein DC. pcDNA–Flag–beclin 1 is C-terminally FLAG tagged - Ref 30 supplement (pFLAG-CMV™-5a -SIGMA) The authors have stated that HA-AR and eGFP-ATN1 (as used in Extended Data figure 10) are of Human origin. dcr1000@cam.ac.uk Accepted 2017-AUG-08 AT 09:41 BST AT 09:41 BST by ORCHARD Nature 2017 imex curation altered both mutations to "decreasing rate" becn1_human Beclin-1 Coiled-coil myosin-like BCL2-interacting protein Protein GT197 BECN1 GT197 protein protein human Homo sapiens Human MEGSKTSNNSTMQVSFVCQRCSQPLKLDTSFKILDRVTIQELTAPLLTTAQAKPGETQEEETNSGEEPFIETPRQDGVSRRFIPPARMMSTESANSFTLIGEASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTDTLLDQLDTQLNVTENECQNYKRCLEILEQMNEDDSEQLQMELKELALEEERLIQELEDVEKNRKIVAENLEKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSVENQMRYAQTQLDKLKKTNVFNATFHIWHSGQFGTINNFRLGRLPSVPVEWNEINAAWGQTVLLLHALANKMGLKFQRYRLVPYGNHSYLESLTDKSKELPLYCSGGLRFFWDNKFDHAMVAFLDCVQQFKEEVEKGETRFCLPYRMDVEKGKIEDTGGSGGSYSIKTQFNSEEQWTKALKFMLTNLKWGLAWVSSQFYNK DIP protein Q14457 original sequence version: 95 DIP protein Q14457 original sequence version: 156 DIP protein Q14457 original sequence version: 122 DIP protein Q14457 original sequence version: 120 DIP protein Q14457 original sequence version: 133 DIP protein Q14457 original sequence version: 141 DIP protein Q14457 original sequence version: 106 ABF0C2DD7087473C hd_human Huntingtin HTT IT15 HD Huntington disease protein protein protein human Homo sapiens Human MATLEKLMKAFESLKSFQQQQQQQQQQQQQQQQQQQQQPPPPPPPPPPPQLPQPPPQAQPLLPQPQPPPPPPPPPPGPAVAEEPLHRPKKELSATKKDRVNHCLTICENIVAQSVRNSPEFQKLLGIAMELFLLCSDDAESDVRMVADECLNKVIKALMDSNLPRLQLELYKEIKKNGAPRSLRAALWRFAELAHLVRPQKCRPYLVNLLPCLTRTSKRPEESVQETLAAAVPKIMASFGNFANDNEIKVLLKAFIANLKSSSPTIRRTAAGSAVSICQHSRRTQYFYSWLLNVLLGLLVPVEDEHSTLLILGVLLTLRYLVPLLQQQVKDTSLKGSFGVTRKEMEVSPSAEQLVQVYELTLHHTQHQDHNVVTGALELLQQLFRTPPPELLQTLTAVGGIGQLTAAKEESGGRSRSGSIVELIAGGGSSCSPVLSRKQKGKVLLGEEEALEDDSESRSDVSSSALTASVKDEISGELAASSGVSTPGSAGHDIITEQPRSQHTLQADSVDLASCDLTSSATDGDEEDILSHSSSQVSAVPSDPAMDLNDGTQASSPISDSSQTTTEGPDSAVTPSDSSEIVLDGTDNQYLGLQIGQPQDEDEEATGILPDEASEAFRNSSMALQQAHLLKNMSHCRQPSDSSVDKFVLRDEATEPGDQENKPCRIKGDIGQSTDDDSAPLVHCVRLLSASFLLTGGKNVLVPDRDVRVSVKALALSCVGAAVALHPESFFSKLYKVPLDTTEYPEEQYVSDILNYIDHGDPQVRGATAILCGTLICSILSRSRFHVGDWMGTIRTLTGNTFSLADCIPLLRKTLKDESSVTCKLACTAVRNCVMSLCSSSYSELGLQLIIDVLTLRNSSYWLVRTELLETLAEIDFRLVSFLEAKAENLHRGAHHYTGLLKLQERVLNNVVIHLLGDEDPRVRHVAAASLIRLVPKLFYKCDQGQADPVVAVARDQSSVYLKLLMHETQPPSHFSVSTITRIYRGYNLLPSITDVTMENNLSRVIAAVSHELITSTTRALTFGCCEALCLLSTAFPVCIWSLGWHCGVPPLSASDESRKSCTVGMATMILTLLSSAWFPLDLSAHQDALILAGNLLAASAPKSLRSSWASEEEANPAATKQEEVWPALGDRALVPMVEQLFSHLLKVINICAHVLDDVAPGPAIKAALPSLTNPPSLSPIRRKGKEKEPGEQASVPLSPKKGSEASAASRQSDTSGPVTTSKSSSLGSFYHLPSYLKLHDVLKATHANYKVTLDLQNSTEKFGGFLRSALDVLSQILELATLQDIGKCVEEILGYLKSCFSREPMMATVCVQQLLKTLFGTNLASQFDGLSSNPSKSQGRAQRLGSSSVRPGLYHYCFMAPYTHFTQALADASLRNMVQAEQENDTSGWFDVLQKVSTQLKTNLTSVTKNRADKNAIHNHIRLFEPLVIKALKQYTTTTCVQLQKQVLDLLAQLVQLRVNYCLLDSDQVFIGFVLKQFEYIEVGQFRESEAIIPNIFFFLVLLSYERYHSKQIIGIPKIIQLCDGIMASGRKAVTHAIPALQPIVHDLFVLRGTNKADAGKELETQKEVVVSMLLRLIQYHQVLEMFILVLQQCHKENEDKWKRLSRQIADIILPMLAKQQMHIDSHEALGVLNTLFEILAPSSLRPVDMLLRSMFVTPNTMASVSTVQLWISGILAILRVLISQSTEDIVLSRIQELSFSPYLISCTVINRLRDGDSTSTLEEHSEGKQIKNLPEETFSRFLLQLVGILLEDIVTKQLKVEMSEQQHTFYCQELGTLLMCLIHIFKSGMFRRITAAATRLFRSDGCGGSFYTLDSLNLRARSMITTHPALVLLWCQILLLVNHTDYRWWAEVQQTPKRHSLSSTKLLSPQMSGEEEDSDLAAKLGMCNREIVRRGALILFCDYVCQNLHDSEHLTWLIVNHIQDLISLSHEPPVQDFISAVHRNSAASGLFIQAIQSRCENLSTPTMLKKTLQCLEGIHLSQSGAVLTLYVDRLLCTPFRVLARMVDILACRRVEMLLAANLQSSMAQLPMEELNRIQEYLQSSGLAQRHQRLYSLLDRFRLSTMQDSLSPSPPVSSHPLDGDGHVSLETVSPDKDWYVHLVKSQCWTRSDSALLEGAELVNRIPAEDMNAFMMNSEFNLSLLAPCLSLGMSEISGGQKSALFEAAREVTLARVSGTVQQLPAVHHVFQPELPAEPAAYWSKLNDLFGDAALYQSLPTLARALAQYLVVVSKLPSHLHLPPEKEKDIVKFVVATLEALSWHLIHEQIPLSLDLQAGLDCCCLALQLPGLWSVVSSTEFVTHACSLIYCVHFILEAVAVQPGEQLLSPERRTNTPKAISEEEEEVDPNTQNPKYITAACEMVAEMVESLQSVLALGHKRNSGVPAFLTPLLRNIIISLARLPLVNSYTRVPPLVWKLGWSPKPGGDFGTAFPEIPVEFLQEKEVFKEFIYRINTLGWTSRTQFEETWATLLGVLVTQPLVMEQEESPPEEDTERTQINVLAVQAITSLVLSAMTVPVAGNPAVSCLEQQPRNKPLKALDTRFGRKLSIIRGIVEQEIQAMVSKRENIATHHLYQAWDPVPSLSPATTGALISHEKLLLQINPERELGSMSYKLGQVSIHSVWLGNSITPLREEEWDEEEEEEADAPAPSSPPTSPVNSRKHRAGVDIHSCSQFLLELYSRWILPSSSARRTPAILISEVVRSLLVVSDLFTERNQFELMYVTLTELRRVHPSEDEILAQYLVPATCKAAAVLGMDKAVAEPVSRLLESTLRSSHLPSRVGALHGVLYVLECDLLDDTAKQLIPVISDYLLSNLKGIAHCVNIHSQQHVLVMCATAFYLIENYPLDVGPEFSASIIQMCGVMLSGSEESTPSIIYHCALRGLERLLLSEQLSRLDAESLVKLSVDRVNVHSPHRAMAALGLMLTCMYTGKEKVSPGRTSDPNPAAPDSESVIVAMERVSVLFDRIRKGFPCEARVVARILPQFLDDFFPPQDIMNKVIGEFLSNQQPYPQFMATVVYKVFQTLHSTGQSSMVRDWVMLSLSNFTQRAPVAMATWSLSCFFVSASTSPWVAAILPHVISRMGKLEQVDVNLFCLVATDFYRHQIEEELDRRAFQSVLEVVAAPGSPYHRLLTCLRNVHKVTTC DIP protein P42858 original sequence version: 148 DIP protein P42858 original sequence version: 145 DIP protein P42858 original sequence version: 142 DIP protein P42858 original sequence version: 157 DIP protein P42858 original sequence version: 158 DIP protein P42858 original sequence version: 156 A267509E84D52F0D atn1_human Atrophin-1 ATN1 DRPLA Dentatorubral-pallidoluysian atrophy protein D12S755E protein protein human Homo sapiens Human MKTRQNKDSMSMRSGRKKEAPGPREELRSRGRASPGGVSTSSSDGKAEKSRQTAKKARVEEASTPKVNKQGRSEEISESESEETNAPKKTKTEQELPRPQSPSDLDSLDGRSLNDDGSSDPRDIDQDNRSTSPSIYSPGSVENDSDSSSGLSQGPARPYHPPPLFPPSPQPPDSTPRQPEASFEPHPSVTPTGYHAPMEPPTSRMFQAPPGAPPPHPQLYPGGTGGVLSGPPMGPKGGGAASSVGGPNGGKQHPPPTTPISVSSSGASGAPPTKPPTTPVGGGNLPSAPPPANFPHVTPNLPPPPALRPLNNASASPPGLGAQPLPGHLPSPHAMGQGMGGLPPGPEKGPTLAPSPHSLPPASSSAPAPPMRFPYSSSSSSSAAASSSSSSSSSSASPFPASQALPSYPHSFPPPTSLSVSNQPPKYTQPSLPSQAVWSQGPPPPPPYGRLLANSNAHPGPFPPSTGAQSTAHPPVSTHHHHHQQQQQQQQQQQQQQQQQQQHHGNSGPPPPGAFPHPLEGGSSHHAHPYAMSPSLGSLRPYPPGPAHLPPPHSQVSYSQAGPNGPPVSSSSNSSSSTSQGSYPCSHPSPSQGPQGAPYPFPPVPTVTTSSATLSTVIATVASSPAGYKTASPPGPPPYGKRAPSPGAYKTATPPGYKPGSPPSFRTGTPPGYRGTSPPAGPGTFKPGSPTVGPGPLPPAGPSGLPSLPPPPAAPASGPPLSATQIKQEPAEEYETPESPVPPARSPSPPPKVVDVPSHASQSARFNKHLDRGFNSCARSDLYFVPLEGSKLAKKRADLVEKVRREAEQRAREEKEREREREREKEREREKERELERSVKLAQEGRAPVECPSLGPVPHRPPFEPGSAVATVPPYLGPDTPALRTLSEYARPHVMSPGNRNHPFYVPLGAVDPGLLGYNVPALYSSDPAAREREREARERDLRDRLKPGFEVKPSELEPLHGVPGPGLDPFPRHGGLALQPGPPGLHPFPFHPSLGPLERERLALAAGPALRPDMSYAERLAAERQHAERVAALGNDPLARLQMLNVTPHHHQHSHIHSHLHLHQQDAIHAASASVHPLIDPLASGSHLTRIPYPAGTLPNPLLPHPLHENEVLRHQLFAAPYRDLPASLSAPMSAAHQLQAMHAQSAELQRLALEQQQWLHAHHPLHSVPLPAQEDYYSHLKKESDKPL B47603486C672637 B47603486C672637 p54252-1 Ataxin-3 ATXN3 ATX3 MJD MJD1 SCA3 Machado-Joseph disease protein 1 Spinocerebellar ataxia type 3 protein MJD1a protein protein human Homo sapiens Human MESIFHEKQEGSLCAQHCLNNLLQGEYFSPVELSSIAHQLDEEERMRMAEGGVTSEDYRTFLQQPSGNMDDSGFFSIQVISNALKVWGLELILFNSPEYQRLRIDPINERSFICNYKEHWFTVRKLGKQWFNLNSLLTGPELISDTYLALFLAQLQQEGYSIFVVKGDLPDCEADQLLQMIRVQQMHRPKLIGEELAQLKEQRVHKTDLERVLEANDGSGMLDEDEEDLQRALALSRQEIDMEDEEADLRRAIQLSMQGSSRNISQDMTQTSGTNLTSEELRKRREAYFEKQQQKQQQQQQQQQQGDLSGQSSHPCERPATSSGALGSDLGKACSPFIMFATFTLYLTYELHVIFALHYSSFPL Variant in position: 349:YELHVIFALHYSSFPL->Missing (in short isoform 1, due to a stop-gain single nucleotide polymorphism, has reduced half-life due to increased proteasomal degradation, has reduced solubility and increased tendency to form aggregates, increased localization to the nucleus). 4B2477EB67C30EFF andr_human Androgen receptor Dihydrotestosterone receptor Nuclear receptor subfamily 3 group C member 4 AR DHTR NR3C4 protein protein human Homo sapiens Human MEVQLGLGRVYPRPPSKTYRGAFQNLFQSVREVIQNPGPRHPEAASAAPPGASLLLLQQQQQQQQQQQQQQQQQQQQQQQETSPRQQQQQQGEDGSPQAHRRGPTGYLVLDEEQQPSQPQSALECHPERGCVPEPGAAVAASKGLPQQLPAPPDEDDSAAPSTLSLLGPTFPGLSSCSADLKDILSEASTMQLLQQQQQEAVSEGSSSGRAREASGAPTSSKDNYLGGTSTISDNAKELCKAVSVSMGLGVEALEHLSPGEQLRGDCMYAPLLGVPPAVRPTPCAPLAECKGSLLDDSAGKSTEDTAEYSPFKGGYTKGLEGESLGCSGSAAAGSSGTLELPSTLSLYKSGALDEAAAYQSRDYYNFPLALAGPPPPPPPPHPHARIKLENPLDYGSAWAAAAAQCRYGDLASLHGAGAAGPGSGSPSAAASSSWHTLFTAEEGQLYGPCGGGGGGGGGGGGGGGGGGGGGGGEAGAVAPYGYTRPPQGLAGQESDFTAPDVWYPGGMVSRVPYPSPTCVKSEMGPWMDSYSGPYGDMRLETARDHVLPIDYYFPPQKTCLICGDEASGCHYGALTCGSCKVFFKRAAEGKQKYLCASRNDCTIDKFRRKNCPSCRLRKCYEAGMTLGARKLKKLGNLKLQEEGEASSTTSPTEETTQKLTVSHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALLSSLNELGERQLVHVVKWAKALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRMYSQCVRMRHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKNPTSCSRRFYQLTKLLDSVQPIARELHQFTFDLLIKSHMVSVDFPEMMAEIISVQVPKILSGKVKPIYFHTQ DIP protein P10275 original sequence version: 220 DIP protein P10275-1 original sequence version: 213 DIP protein P10275 original sequence version: 216 DIP protein P10275 original sequence version: 143 DIP protein P10275 original sequence version: 174 DIP protein P10275 original sequence version: 169 DIP protein P10275 original sequence version: 188 DIP protein P10275-1 original sequence version: 188 DIP protein P10275 original sequence version: 196 DIP protein P10275 original sequence version: 217 DIP protein P10275-1 original sequence version: 217 DIP protein P10275-1 original sequence version: 207 A73432C55D39AE06 isolated polyq stretch_q35 peptide peptide polypeptide oligopeptide oligopeptide polypeptide human Homo sapiens Human QQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQ isolated polyq stretch_q81 protein protein human Homo sapiens Human QQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQ ataz-bec-1 4 8 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association 2A atxn3-becn1-1 1 5 enzyme target enzyme target substrate substrate neutral component neutral component purified purified HA-tagged ubiquitinylated lysine ubiquitinylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 8 enzyme enzyme neutral component neutral component purified purified P54252-1:p.Gln296[19] q22_poly-glutamine_extension mutation mutation range range range range QQQQQQQQQQ QQQQQQQQQQQQQQQQQQQ Fri Jun 30 13:53:41 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'q22_poly-glutamine_extension' Wed May 02 16:02:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'q22 poly-glutamine extension' Tue Jun 30 17:27:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln296[19]' P54252-1:p.Cys14Ala mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain C A Wed May 02 14:59:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys14ala' Tue Jun 30 16:30:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys14Ala' n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range P54252-1:p.Gln296[77] q80 poly-glutamine extension mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate range range range range QQQQQQQQQQ QQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQ Tue Jun 30 17:16:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln296[77]' human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 deubiquitination deubiquitination reaction deubiquitination 2B, Extended Data fig 9A AT3Q22 is a non-pathogenic form of ATX3 (In normal individuals, the ATXN3 gene was found to contain between 13 and 36 CAG repeats.) -from Omim. why does a poly-glutamine tract have a "K" in the middle? I have excluded this residue in counting my 22 "Qs" (I am assuming the UniProt sequence has the minimum 13 "Qs" present-see comment.) Expansion of the polyQ domain in ataxin 3 decreased its deubiquitinase activity towards beclin 1 Expansion of the polyQ domain in ataxin 3 decreased its deubiquitinase activity towards beclin 1. P54252 (GO:0101005) up-regulates quantity by stabilization Q14457 atxn3-becn1-2 2 5 unspecified role unspecified role prey prey purified purified c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P54252-1:p.Gln296[25] q28_poly-glutamine_extension mutation mutation range range range range QQQQQQQQQQ QQQQQQQQQQQQQQQQQQQQQQQQQ Fri Jun 30 13:56:40 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'q28_poly-glutamine_extension' Wed May 02 20:02:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'q28 poly-glutamine extension' Tue Jun 30 16:15:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln296[25]' n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 3A Ataxin3Q28 is a non-pathogenic form of ATX3 (In normal individuals, the ATXN3 gene was found to contain between 13 and 36 CAG repeats.) -from Omim. Deletion of the polyQ domain from ataxin 3 greatly reduced its binding to beclin 1 (Fig. 3a) Deletion of the polyQ domain from ataxin 3 greatly reduced its binding to beclin 1 (Fig. 3a) the 1-290 construct entered as sufficient to bind + comment, rather than "required to bind" as it is stated there are other binding domains, apparently. The polyQ domain is important for the ataxin 3–beclin 1 interaction, but is not the only binding domain. atxn3-becn1-3 2 5 unspecified role unspecified role prey prey purified purified c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role bait bait purified purified c_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 3B Ataxin3Q28 is a non-pathogenic form of ATX3 (In normal individuals, the ATXN3 gene was found to contain between 13 and 36 CAG repeats.) -from Omim. Deletion of the polyQ domain from ataxin 3 greatly reduced its binding to beclin 1 (Fig. 3a) Deletion of the polyQ domain from ataxin 3 greatly reduced its binding to beclin 1 (Fig. 3a) the 1-290 construct entered as sufficient to bind + comment rather than "required to bind" as it is stated there are other binding domains. becn1-atxn3-1 2 5 unspecified role unspecified role bait bait purified purified c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd_region sufficient to bind sufficient binding region sufficient to bind range range range range 8 unspecified role unspecified role prey prey purified purified P54252-1:p.Gln296[25] q28_poly-glutamine_extension mutation mutation range range range range QQQQQQQQQQ QQQQQQQQQQQQQQQQQQQQQQQQQ Fri Jun 30 14:07:49 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'q28_poly-glutamine_extension' Wed May 02 15:43:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'q28 poly-glutamine extension' Tue Jun 30 17:22:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln296[25]' n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 3C Ataxin3Q28 is a non-pathogenic form of ATX3 (In normal individuals, the ATXN3 gene was found to contain between 13 and 36 CAG repeats.) -from Omim. isolated polyq stretch_q35-becn1-1 3 5 unspecified role unspecified role bait bait purified purified K48_poly-ubiquitination ubiquitinylated lysine ubiquitinylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined resulting-ptm resulting-ptm resulting ptm resulting-ptm 10 unspecified role unspecified role prey prey purified purified isolated polyq stretch_q35:p.Gln35[47] q81 poly-glutamine extension mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Q QQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQ Fri Jun 30 14:04:59 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'q81_poly-glutamine_extension' Tue Jun 30 18:54:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln35[47]' c_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 4A Beclin 1 K48-polyubiquitination was increased in cells expressing GFP–Q81 as compared to cells expressing GFP–Q35. becn1-atxn3-3 3 8 unspecified role unspecified role prey prey purified purified P54252-1:p.Gln296[19] q22_poly-glutamine_extension mutation mutation range range range range QQQQQQQQQQ QQQQQQQQQQQQQQQQQQQ Fri Jun 30 13:52:36 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'q22_poly-glutamine_extension' Wed May 02 18:12:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'q22 poly-glutamine extension' Tue Jun 30 15:36:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln296[19]' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait purified purified physical association physical association 4Bi Ataxin3Q28 is a non-pathogenic form of ATX3 (In normal individuals, the ATXN3 gene was found to contain between 13 and 36 CAG repeats.) -from Omim. becn1-atxn3-4 3 10 competitor competitor competitor unspecified role unspecified role 8 unspecified role unspecified role prey prey purified purified P54252-1:p.Gln296[19] q22 poly-glutamine extension mutation mutation range range range range QQQQQQQQQQ QQQQQQQQQQQQQQQQQQQ Mon Jul 03 14:30:19 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'q22 poly-glutamine extension' Wed May 02 15:53:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'q22 poly-glutamine extension' Tue Jun 30 17:29:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln296[19]' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait purified purified physical association physical association 4Bii Ataxin3Q28 is a non-pathogenic form of ATX3 (In normal individuals, the ATXN3 gene was found to contain between 13 and 36 CAG repeats.) -from Omim. becn1-atxn3-5 3 11 competitor competitor competitor unspecified role unspecified role 8 unspecified role unspecified role prey prey purified purified P54252-1:p.Gln292_Gln305delinsGlnGlnGlnLysGlnGlnGlnGlnGlnGlnGlnGlnGlnGlnGlnGlnGlnGlnGlnGlnGlnGlnGln mutation mutation range range range range QQQKQQQQQQQQQQ QQQKQQQQQQQQQQQQQQQQQQQ Fri Jun 30 14:06:21 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'q22_poly-glutamine_extension' Wed May 02 16:19:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'q22 poly-glutamine extension' Tue Jun 30 20:13:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln292_Gln305delinsGlnGlnGlnLysGlnGlnGlnGlnGlnGlnGlnGlnGlnGlnGlnGlnGlnGlnGlnGlnGlnGlnGln' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait purified purified physical association physical association 4Biii Ataxin3Q28 is a non-pathogenic form of ATX3 (In normal individuals, the ATXN3 gene was found to contain between 13 and 36 CAG repeats.) -from Omim. becn1-atxn3-2 2 5 unspecified role unspecified role bait bait purified purified c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd_region sufficient to bind sufficient binding region sufficient to bind range range range range not_the_ecd_region sufficient to bind sufficient binding region sufficient to bind range range range range 10 unspecified role unspecified role prey prey purified purified c_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Extended Data 5A Ataxin3Q28 is a non-pathogenic form of ATX3 (In normal individuals, the ATXN3 gene was found to contain between 13 and 36 CAG repeats.) -from Omim. becn1-htt-1 3 5 unspecified role unspecified role bait bait purified purified 6 unspecified role unspecified role prey prey purified purified p.Gln35_Gln38del q17 poly-glutamine extension mutation mutation range range range range QQQQ . c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain poly_Q_region required to bind necessary binding region required to bind range range range range physical association physical association Extended Data fig 7A A wild-type (Q17) huntingtin fragment (N-terminal 350 residues) bound to beclin 1, and this interaction was largely lost when the polyQ stretch was deleted. A wild-type (Q17) huntingtin fragment (N-terminal 350 residues) bound to beclin 1, and this interaction was largely lost when the polyQ stretch was deleted. I cannot find any details for DeltaQ construct (HTT) becn1-htt-2 3 5 unspecified role unspecified role bait bait purified purified 8 unspecified role unspecified role prey prey P54252-1:p.Gln296[28] q28_poly-glutamine_extension[292..292-305..305 mutation mutation range range range range Q QQQQQQQQQQQQQQQQQQQQQQQQQQQQ Fri Jun 30 14:08:14 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'q28_poly-glutamine_extension[292..292-305..305]' Wed May 02 17:30:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'q28 poly-glutamine extension' Wed Jun 13 17:31:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln296[25]' Tue Jun 30 20:20:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln296[28]' n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association Extended Data fig 7B becn1-atxn3-6 3 6 competitor competitor competitor prey prey purified purified P42858:p.Gln18[138] mutation mutation range range range range QQQQQQQQQQQQQQQQQQQQQ QQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQ Wed May 02 15:13:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'q138 poly-glutamine extension' Tue Jun 30 18:45:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln18[138]' c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 unspecified role unspecified role bait bait purified purified 8 unspecified role unspecified role prey prey P54252-1:p.Gln296[25] q28_poly-glutamine_extension mutation mutation range range range range QQQQQQQQQQ QQQQQQQQQQQQQQQQQQQQQQQQQ Fri Jun 30 14:06:01 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'q28_poly-glutamine_extension' Wed May 02 18:01:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'q28 poly-glutamine extension' Tue Jun 30 19:01:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln296[25]' n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association Extended Data fig 7Bi atxn3-becn1-4 4 5 unspecified role unspecified role bait bait purified purified K48_poly-ubiquitination ubiquitinylated lysine ubiquitinylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined resulting-ptm resulting-ptm resulting ptm resulting-ptm 8 unspecified role unspecified role prey prey purified purified P54252-1:p.Gln296[19] q22_poly-glutamine_extension' mutation mutation range range range range QQQQQQQQQQ QQQQQQQQQQQQQQQQQQQ Fri Jun 30 14:19:27 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'q22_poly-glutamine_extension' Wed May 02 17:13:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'q22 poly-glutamine extension' Tue Jun 30 17:50:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln296[19]' n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range P54252-1:p.Gln296[77] q80 poly-glutamine extension mutation increasing strength mutation increasing interaction strength mutation increasing strength range range range range QQQQQQQQQQ QQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQ Tue Jun 30 17:59:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln296[77]' physical association physical association Extended Data fig 9D AT3Q22 is a non-pathogenic form of ATX3 (In normal individuals, the ATXN3 gene was found to contain between 13 and 36 CAG repeats.) -from Omim. why does a poly-glutamine tract have a "K" in the middle? I have excluded this residue in counting my 22 "Qs" (I am assuming the UniProt sequence has the minimum 13 "Qs" present-see comment.) Q84 mutant ataxin 3 interacted more strongly with beclin 1 than did wild-type ataxin 3, and this increased interaction was associated with increased beclin 1 K48 polyubiquitination Q80 mutant ataxin 3 interacted more strongly with beclin 1 than did wild-type ataxin 3, and this increased interaction was associated with increased beclin 1 K48 polyubiquitination. becn1-atn1-1 3 5 unspecified role unspecified role bait bait purified purified K48_poly-ubiquitination ubiquitinylated lysine ubiquitinylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined resulting-ptm resulting-ptm resulting ptm resulting-ptm 7 unspecified role unspecified role prey prey region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P54259:p.Gln484[71] mutation increasing strength mutation increasing interaction strength mutation increasing strength range range range range QQQQQQQQQQQQQQQQQQQ QQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQ Wed May 02 14:38:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'q71 poly-glutamine extension' Tue Jun 30 18:21:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln484[71]' n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association Extended Data fig 10A the UniProt version has 19Qs already. becn1-ar-1 3 5 unspecified role unspecified role bait bait purified purified K48_poly-ubiquitination ubiquitinylated lysine ubiquitinylated lysine undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined resulting-ptm resulting-ptm resulting ptm resulting-ptm 9 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p.Gln88_Gln90del q25 poly-glutamine extension mutation mutation range range range range QQQ . n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P10275:p.Gln86[97] q120 poly-glutamine extension mutation increasing strength mutation increasing interaction strength mutation increasing strength range range range range QQQQQ QQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQ Wed Jun 13 18:56:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.gln86[97]' Tue Jun 30 17:15:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln86[97]' association association Extended Data fig 10B The location of the Q25 and Q120 polyglutamine repeats is not known;there are three glutamine rich regions in AR.