UniProt UniProt http://www.uniprot.org G protein-coupled receptor kinase-2 (GRK-2) regulates serotonin metabolism through the monoamine oxidase AMX-2 in Caenorhabditis elegans. G protein-coupled receptor kinase-2 (GRK-2) regulates serotonin metabolism through the monoamine oxidase AMX-2 in Caenorhabditis elegans. The Journal of biological chemistry 2017 imex curation Wang J., Luo J., Aryal DK., Wetsel WC., Nass R., Benovic JL. jeffrey.benovic@jefferson.edu 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation caeel Caenorhabditis elegans anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang J., Luo J., Aryal DK., Wetsel WC., Nass R., Benovic JL. jeffrey.benovic@jefferson.edu Accepted 2017-NOV-09 AT 12:07 GMT AT 12:07 GMT by LIVIAP The Journal of biological chemistry 2017 imex curation grk2_caeel G protein-coupled receptor kinase 2 grk-2 W02B3.2 protein protein caeel Caenorhabditis elegans MADLEAVLADVSYLMAMEKSRSQPAARASKRIVLPDPSVRSIMQKFLEKSGDMKFDKIFNQKLGFLLLKDYAENVSESPCPQIKFYEAIKEYEKMETPDERLTKAREIYDHHIMVEMLAHAHNYSKESLQHVQYHLLKQNVPPDLFHRYVLEICDQLRGDIFQRFLESDKFTRFCQWKNLELNMQLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGQDCPFIVCMTYAFQSPDKLCFILDLMNGGDLHYHLSQHGVFTEQEMIFYASEVILGLEHMHNRFVVYRDLKPANILLDENGHVRVSDLGLACDYSKKKPHASVGTHGYMAPEVLAKGVAYDSSADWFSLGCMLYKLLKGHSPFRQHKSKDKNEIDKMTLTQDIELPNEGLSKDCRDLLEGLLKRDVPDRLGCRGKGPTEVKEHPFFKDVDWQTVYLRRMTPPLIPPRGEVNAADAFDIGNFDDDEVKGVKLQDGDSDLYKNFNIVISERWQNEIAETIFEVVNQDADKAESKKRSKQKIKVAVEEKDSDVIVHGYIKKLGGPFTSAWQTKYGKLYPSRLELYPESLTAKPELVFMDQIEDVCAEMQTIKGETAIIVKLRDGFKEPKICLTNSDEISLKEWHTSLRTAHKVSQELLQRMGRKAIKIYGVNHDPMLSESERPGSVTRAFLNRASSVDSGV mint Specifically phosphorylates the activated forms of G protein-coupled receptors D37F03260F324939 q9xxu5_caeel amx-2 B0019.1 CELE_B0019.1 protein protein caeel Caenorhabditis elegans MLRGWWIFIWAAVCVYAQNTLSQQSQQQQQPTSANISSSNIQQTIYDVIVVGAGLTGLTAARNIQQNRPGLSVLVLEARGQVGGRIRYATMQTRNGVEFVDTGSQFISPTDTQLLSLIQELNVRTTQQLTCGNNTVFQQTRKKRQLSLQQQWSTTLFTDLINSPETLGNLTNTSVSAMSQQMDTADADSVNRMMQTFFDAPGEQVPEIQLALTCSSQNATAVEILRRFGHGQSLLAQGGMNEVVRRLADGLLIEYSQRVVSVNDAAFPAVVQTSAGRRFSGRQVIVAVPIPTLENIELVPAPEAPFQQLIQNYGPTGHAYYFTMSFQRATWRLNGRSGKVIYTSATGPLVWLTTFDTTFAASCDNSTSASSTLWGIAHFSYDVPFETRRKLYTQAIMYSLRFADFSPLDVSDVNFATDDLAKGTIPTLKLNIPLESLKYLNDFHTLYQNVHIATADIASHNLGTMNGAVHAAGSVSTYVLQMLSAADAQSNGVLRDTPVESTTPYVYSTSSHYPPSLFAARNVQNDSGNRNTTGNNSVNSGNTGNQTNFQYETSSQYPTTVETPTTTTMKHFSFGNLDNSTNEEPAAVAYDSPQQDAPPTLNYSTTFAYSTSSVMPLVETIETTTFKHFSNGNLDQNATSAVQRDVPAVLVNQQQNGYAYTTSTHYPVQLSSNRNRIDSRATPSPQVVQELQQVSANASNSTALQLASSLTQLVQTLLQTLRLQ 98381AD057D8418E amx_2-grk_2-1 1 2 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 3 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level region opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined observed-ptm observed-ptm observed ptm observed-ptm physical association physical association Interestingly, immunoprecipitation of AMX-2 resulted in significant co-immunoprecipitation of GRK-2, and this was modestly enhanced in the presence of aluminum fluoride, which activates heterotrimeric G proteins (Fig. 7B). Aluminium fluoride (CHEBI:66871) Fig. 7B, 7C