IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A novel PHD-finger protein 14/KIF4A complex overexpressed in lung cancer is involved in cell mitosis regulation and tumorigenesis. A novel PHD-finger protein 14/KIF4A complex overexpressed in lung cancer is involved in cell mitosis regulation and tumorigenesis. Oncotarget 2017 imex curation Zhang L., Huang Q., Lou J., Zou L., Wang Y., Zhang P., Yang G., Zhang J., Yu L., Yan D., Zhang C., Qiao J., Wang S., Wang S., Xu Y., Ji H., Chen Z., Zhang Z. The full-length human PHF14 construct given in Reference 12 (pmid:23688586) refers to Ref-Seq protein NP_001007158.1 (PHF14-alpha isoform of 948 aas). It is stated:- "This RefSeq was permanently suppressed because currently there is insufficient support for the transcript." The alpha and beta PHF14 isoforms have an identical sequence but the alpha form has an extended C-terminus. zhangzhe@sibcb.ac.cn,zjchen@sibcb.ac.cn 14-Feb-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhang L., Huang Q., Lou J., Zou L., Wang Y., Zhang P., Yang G., Zhang J., Yu L., Yan D., Zhang C., Qiao J., Wang S., Wang S., Xu Y., Ji H., Chen Z., Zhang Z. The full-length human PHF14 construct given in Reference 12 (pmid:23688586) refers to Ref-Seq protein NP_001007158.1 (PHF14-alpha isoform of 948 aas). It is stated:- "This RefSeq was permanently suppressed because currently there is insufficient support for the transcript." The alpha and beta PHF14 isoforms have an identical sequence but the alpha form has an extended C-terminus. zhangzhe@sibcb.ac.cn,zjchen@sibcb.ac.cn Accepted 2018-JAN-10 AT 11:59 GMT AT 11:59 GMT by LIVIAP Oncotarget 2017 imex curation We have in the past been told only ever to enter Associations for Chips, Co-Ips and Pull-Downs + other child terms of Affinity Chromatography- not for anything else; see section 5.4.1 in the Manual. Gel filtration is obviously not "co-localisation" as the definition from OLS for this is "Coincident occurrence of molecules in a given subcellular fraction observed with a low resolution methodology from which a physical interaction among those molecules cannot be inferred." This only leaves "physical Assoc"(- I dont know what/where "Direct" terms in the Database are from). Do we need to discuss this further - +Pablo/Sandra? I notice the bulk of the term "Association" for Mol. Seiving have been used by Mint - so I think there is a difference in what we are doing here at IntAct. A novel PHD-finger protein 14/KIF4A complex overexpressed in lung cancer is involved in cell mitosis regulation and tumorigenesis. A novel PHD-finger protein 14/KIF4A complex overexpressed in lung cancer is involved in cell mitosis regulation and tumorigenesis. Oncotarget 2017 imex curation Zhang L., Huang Q., Lou J., Zou L., Wang Y., Zhang P., Yang G., Zhang J., Yu L., Yan D., Zhang C., Qiao J., Wang S., Wang S., Xu Y., Ji H., Chen Z., Zhang Z. The full-length human PHF14 construct given in Reference 12 (pmid:23688586) refers to Ref-Seq protein NP_001007158.1 (PHF14-alpha isoform of 948 aas). It is stated:- "This RefSeq was permanently suppressed because currently there is insufficient support for the transcript." The alpha and beta PHF14 isoforms have an identical sequence but the alpha form has an extended C-terminus. zhangzhe@sibcb.ac.cn,zjchen@sibcb.ac.cn 14-Feb-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Zhang L., Huang Q., Lou J., Zou L., Wang Y., Zhang P., Yang G., Zhang J., Yu L., Yan D., Zhang C., Qiao J., Wang S., Wang S., Xu Y., Ji H., Chen Z., Zhang Z. The full-length human PHF14 construct given in Reference 12 (pmid:23688586) refers to Ref-Seq protein NP_001007158.1 (PHF14-alpha isoform of 948 aas). It is stated:- "This RefSeq was permanently suppressed because currently there is insufficient support for the transcript." The alpha and beta PHF14 isoforms have an identical sequence but the alpha form has an extended C-terminus. zhangzhe@sibcb.ac.cn,zjchen@sibcb.ac.cn Accepted 2018-JAN-10 AT 11:59 GMT AT 11:59 GMT by LIVIAP Oncotarget 2017 imex curation We have in the past been told only ever to enter Associations for Chips, Co-Ips and Pull-Downs + other child terms of Affinity Chromatography- not for anything else; see section 5.4.1 in the Manual. Gel filtration is obviously not "co-localisation" as the definition from OLS for this is "Coincident occurrence of molecules in a given subcellular fraction observed with a low resolution methodology from which a physical interaction among those molecules cannot be inferred." This only leaves "physical Assoc"(- I dont know what/where "Direct" terms in the Database are from). Do we need to discuss this further - +Pablo/Sandra? I notice the bulk of the term "Association" for Mol. Seiving have been used by Mint - so I think there is a difference in what we are doing here at IntAct. A novel PHD-finger protein 14/KIF4A complex overexpressed in lung cancer is involved in cell mitosis regulation and tumorigenesis. A novel PHD-finger protein 14/KIF4A complex overexpressed in lung cancer is involved in cell mitosis regulation and tumorigenesis. Oncotarget 2017 imex curation Zhang L., Huang Q., Lou J., Zou L., Wang Y., Zhang P., Yang G., Zhang J., Yu L., Yan D., Zhang C., Qiao J., Wang S., Wang S., Xu Y., Ji H., Chen Z., Zhang Z. The full-length human PHF14 construct given in Reference 12 (pmid:23688586) refers to Ref-Seq protein NP_001007158.1 (PHF14-alpha isoform of 948 aas). It is stated:- "This RefSeq was permanently suppressed because currently there is insufficient support for the transcript." The alpha and beta PHF14 isoforms have an identical sequence but the alpha form has an extended C-terminus. zhangzhe@sibcb.ac.cn,zjchen@sibcb.ac.cn 14-Feb-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Zhang L., Huang Q., Lou J., Zou L., Wang Y., Zhang P., Yang G., Zhang J., Yu L., Yan D., Zhang C., Qiao J., Wang S., Wang S., Xu Y., Ji H., Chen Z., Zhang Z. The full-length human PHF14 construct given in Reference 12 (pmid:23688586) refers to Ref-Seq protein NP_001007158.1 (PHF14-alpha isoform of 948 aas). It is stated:- "This RefSeq was permanently suppressed because currently there is insufficient support for the transcript." The alpha and beta PHF14 isoforms have an identical sequence but the alpha form has an extended C-terminus. zhangzhe@sibcb.ac.cn,zjchen@sibcb.ac.cn Accepted 2018-JAN-10 AT 11:59 GMT AT 11:59 GMT by LIVIAP Oncotarget 2017 imex curation We have in the past been told only ever to enter Associations for Chips, Co-Ips and Pull-Downs + other child terms of Affinity Chromatography- not for anything else; see section 5.4.1 in the Manual. Gel filtration is obviously not "co-localisation" as the definition from OLS for this is "Coincident occurrence of molecules in a given subcellular fraction observed with a low resolution methodology from which a physical interaction among those molecules cannot be inferred." This only leaves "physical Assoc"(- I dont know what/where "Direct" terms in the Database are from). Do we need to discuss this further - +Pablo/Sandra? I notice the bulk of the term "Association" for Mol. Seiving have been used by Mint - so I think there is a difference in what we are doing here at IntAct. A novel PHD-finger protein 14/KIF4A complex overexpressed in lung cancer is involved in cell mitosis regulation and tumorigenesis. A novel PHD-finger protein 14/KIF4A complex overexpressed in lung cancer is involved in cell mitosis regulation and tumorigenesis. Oncotarget 2017 imex curation Zhang L., Huang Q., Lou J., Zou L., Wang Y., Zhang P., Yang G., Zhang J., Yu L., Yan D., Zhang C., Qiao J., Wang S., Wang S., Xu Y., Ji H., Chen Z., Zhang Z. The full-length human PHF14 construct given in Reference 12 (pmid:23688586) refers to Ref-Seq protein NP_001007158.1 (PHF14-alpha isoform of 948 aas). It is stated:- "This RefSeq was permanently suppressed because currently there is insufficient support for the transcript." The alpha and beta PHF14 isoforms have an identical sequence but the alpha form has an extended C-terminus. zhangzhe@sibcb.ac.cn,zjchen@sibcb.ac.cn 14-Feb-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Zhang L., Huang Q., Lou J., Zou L., Wang Y., Zhang P., Yang G., Zhang J., Yu L., Yan D., Zhang C., Qiao J., Wang S., Wang S., Xu Y., Ji H., Chen Z., Zhang Z. The full-length human PHF14 construct given in Reference 12 (pmid:23688586) refers to Ref-Seq protein NP_001007158.1 (PHF14-alpha isoform of 948 aas). It is stated:- "This RefSeq was permanently suppressed because currently there is insufficient support for the transcript." The alpha and beta PHF14 isoforms have an identical sequence but the alpha form has an extended C-terminus. zhangzhe@sibcb.ac.cn,zjchen@sibcb.ac.cn Accepted 2018-JAN-10 AT 11:59 GMT AT 11:59 GMT by LIVIAP Oncotarget 2017 imex curation We have in the past been told only ever to enter Associations for Chips, Co-Ips and Pull-Downs + other child terms of Affinity Chromatography- not for anything else; see section 5.4.1 in the Manual. Gel filtration is obviously not "co-localisation" as the definition from OLS for this is "Coincident occurrence of molecules in a given subcellular fraction observed with a low resolution methodology from which a physical interaction among those molecules cannot be inferred." This only leaves "physical Assoc"(- I dont know what/where "Direct" terms in the Database are from). Do we need to discuss this further - +Pablo/Sandra? I notice the bulk of the term "Association" for Mol. Seiving have been used by Mint - so I think there is a difference in what we are doing here at IntAct. A novel PHD-finger protein 14/KIF4A complex overexpressed in lung cancer is involved in cell mitosis regulation and tumorigenesis. A novel PHD-finger protein 14/KIF4A complex overexpressed in lung cancer is involved in cell mitosis regulation and tumorigenesis. Oncotarget 2017 imex curation Zhang L., Huang Q., Lou J., Zou L., Wang Y., Zhang P., Yang G., Zhang J., Yu L., Yan D., Zhang C., Qiao J., Wang S., Wang S., Xu Y., Ji H., Chen Z., Zhang Z. The full-length human PHF14 construct given in Reference 12 (pmid:23688586) refers to Ref-Seq protein NP_001007158.1 (PHF14-alpha isoform of 948 aas). It is stated:- "This RefSeq was permanently suppressed because currently there is insufficient support for the transcript." The alpha and beta PHF14 isoforms have an identical sequence but the alpha form has an extended C-terminus. zhangzhe@sibcb.ac.cn,zjchen@sibcb.ac.cn 14-Feb-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Zhang L., Huang Q., Lou J., Zou L., Wang Y., Zhang P., Yang G., Zhang J., Yu L., Yan D., Zhang C., Qiao J., Wang S., Wang S., Xu Y., Ji H., Chen Z., Zhang Z. HeLa cell nuclei were lysed and separated by gel filtration (fast protein liquid chromatography-FPLC). The full-length human PHF14 construct given in Reference 12 (pmid:23688586) refers to Ref-Seq protein NP_001007158.1 (PHF14-alpha isoform of 948 aas). It is stated:- "This RefSeq was permanently suppressed because currently there is insufficient support for the transcript." The alpha and beta PHF14 isoforms have an identical sequence but the alpha form has an extended C-terminus. zhangzhe@sibcb.ac.cn,zjchen@sibcb.ac.cn Accepted 2018-JAN-10 AT 11:59 GMT AT 11:59 GMT by LIVIAP Oncotarget 2017 imex curation We have in the past been told only ever to enter Associations for Chips, Co-Ips and Pull-Downs + other child terms of Affinity Chromatography- not for anything else; see section 5.4.1 in the Manual. Gel filtration is obviously not "co-localisation" as the definition from OLS for this is "Coincident occurrence of molecules in a given subcellular fraction observed with a low resolution methodology from which a physical interaction among those molecules cannot be inferred." This only leaves "physical Assoc"(- I dont know what/where "Direct" terms in the Database are from). Do we need to discuss this further - +Pablo/Sandra? I notice the bulk of the term "Association" for Mol. Seiving have been used by Mint - so I think there is a difference in what we are doing here at IntAct. A novel PHD-finger protein 14/KIF4A complex overexpressed in lung cancer is involved in cell mitosis regulation and tumorigenesis. A novel PHD-finger protein 14/KIF4A complex overexpressed in lung cancer is involved in cell mitosis regulation and tumorigenesis. Oncotarget 2017 imex curation Zhang L., Huang Q., Lou J., Zou L., Wang Y., Zhang P., Yang G., Zhang J., Yu L., Yan D., Zhang C., Qiao J., Wang S., Wang S., Xu Y., Ji H., Chen Z., Zhang Z. The full-length human PHF14 construct given in Reference 12 (pmid:23688586) refers to Ref-Seq protein NP_001007158.1 (PHF14-alpha isoform of 948 aas). It is stated:- "This RefSeq was permanently suppressed because currently there is insufficient support for the transcript." The alpha and beta PHF14 isoforms have an identical sequence but the alpha form has an extended C-terminus. zhangzhe@sibcb.ac.cn,zjchen@sibcb.ac.cn 14-Feb-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Zhang L., Huang Q., Lou J., Zou L., Wang Y., Zhang P., Yang G., Zhang J., Yu L., Yan D., Zhang C., Qiao J., Wang S., Wang S., Xu Y., Ji H., Chen Z., Zhang Z. The full-length human PHF14 construct given in Reference 12 (pmid:23688586) refers to Ref-Seq protein NP_001007158.1 (PHF14-alpha isoform of 948 aas). It is stated:- "This RefSeq was permanently suppressed because currently there is insufficient support for the transcript." The alpha and beta PHF14 isoforms have an identical sequence but the alpha form has an extended C-terminus. zhangzhe@sibcb.ac.cn,zjchen@sibcb.ac.cn Accepted 2018-JAN-10 AT 11:59 GMT AT 11:59 GMT by LIVIAP Oncotarget 2017 imex curation We have in the past been told only ever to enter Associations for Chips, Co-Ips and Pull-Downs + other child terms of Affinity Chromatography- not for anything else; see section 5.4.1 in the Manual. Gel filtration is obviously not "co-localisation" as the definition from OLS for this is "Coincident occurrence of molecules in a given subcellular fraction observed with a low resolution methodology from which a physical interaction among those molecules cannot be inferred." This only leaves "physical Assoc"(- I dont know what/where "Direct" terms in the Database are from). Do we need to discuss this further - +Pablo/Sandra? I notice the bulk of the term "Association" for Mol. Seiving have been used by Mint - so I think there is a difference in what we are doing here at IntAct. A novel PHD-finger protein 14/KIF4A complex overexpressed in lung cancer is involved in cell mitosis regulation and tumorigenesis. A novel PHD-finger protein 14/KIF4A complex overexpressed in lung cancer is involved in cell mitosis regulation and tumorigenesis. Oncotarget 2017 imex curation Zhang L., Huang Q., Lou J., Zou L., Wang Y., Zhang P., Yang G., Zhang J., Yu L., Yan D., Zhang C., Qiao J., Wang S., Wang S., Xu Y., Ji H., Chen Z., Zhang Z. The full-length human PHF14 construct given in Reference 12 (pmid:23688586) refers to Ref-Seq protein NP_001007158.1 (PHF14-alpha isoform of 948 aas). It is stated:- "This RefSeq was permanently suppressed because currently there is insufficient support for the transcript." The alpha and beta PHF14 isoforms have an identical sequence but the alpha form has an extended C-terminus. zhangzhe@sibcb.ac.cn,zjchen@sibcb.ac.cn 14-Feb-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Zhang L., Huang Q., Lou J., Zou L., Wang Y., Zhang P., Yang G., Zhang J., Yu L., Yan D., Zhang C., Qiao J., Wang S., Wang S., Xu Y., Ji H., Chen Z., Zhang Z. The full-length human PHF14 construct given in Reference 12 (pmid:23688586) refers to Ref-Seq protein NP_001007158.1 (PHF14-alpha isoform of 948 aas). It is stated:- "This RefSeq was permanently suppressed because currently there is insufficient support for the transcript." The alpha and beta PHF14 isoforms have an identical sequence but the alpha form has an extended C-terminus. zhangzhe@sibcb.ac.cn,zjchen@sibcb.ac.cn Accepted 2018-JAN-10 AT 11:59 GMT AT 11:59 GMT by LIVIAP Oncotarget 2017 imex curation We have in the past been told only ever to enter Associations for Chips, Co-Ips and Pull-Downs + other child terms of Affinity Chromatography- not for anything else; see section 5.4.1 in the Manual. Gel filtration is obviously not "co-localisation" as the definition from OLS for this is "Coincident occurrence of molecules in a given subcellular fraction observed with a low resolution methodology from which a physical interaction among those molecules cannot be inferred." This only leaves "physical Assoc"(- I dont know what/where "Direct" terms in the Database are from). Do we need to discuss this further - +Pablo/Sandra? I notice the bulk of the term "Association" for Mol. Seiving have been used by Mint - so I think there is a difference in what we are doing here at IntAct. ccnd1_human G1/S-specific cyclin-D1 CCND1 BCL1 PRAD1 PRAD1 oncogene BCL-1 oncogene B-cell lymphoma 1 protein protein protein human Homo sapiens Human MEHQLLCCEVETIRRAYPDANLLNDRVLRAMLKAEETCAPSVSYFKCVQKEVLPSMRKIVATWMLEVCEEQKCEEEVFPLAMNYLDRFLSLEPVKKSRLQLLGATCMFVASKMKETIPLTAEKLCIYTDNSIRPEELLQMELLLVNKLKWNLAAMTPHDFIEHFLSKMPEAEENKQIIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI DIP protein P24385 original sequence version: 163 DIP protein P24385 original sequence version: 165 DIP protein P24385 original sequence version: 160 DIP protein P24385 original sequence version: 121 DIP protein P24385 original sequence version: 122 DIP protein P24385 original sequence version: 143 DIP protein P24385 original sequence version: 127 3CC00C9905F58D3A kif4a_human Chromosome-associated kinesin KIF4A KIF4A KIF4 Chromokinesin-A protein protein human Homo sapiens Human MKEEVKGIPVRVALRCRPLVPKEISEGCQMCLSFVPGEPQVVVGTDKSFTYDFVFDPSTEQEEVFNTAVAPLIKGVFKGYNATVLAYGQTGSGKTYSMGGAYTAEQENEPTVGVIPRVIQLLFKEIDKKSDFEFTLKVSYLEIYNEEILDLLCPSREKAQINIREDPKEGIKIVGLTEKTVLVALDTVSCLEQGNNSRTVASTAMNSQSSRSHAIFTISLEQRKKSDKNSSFRSKLHLVDLAGSERQKKTKAEGDRLKEGININRGLLCLGNVISALGDDKKGGFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNLEETLNTLRYADRARKIKNKPIVNIDPQTAELNHLKQQVQQLQVLLLQAHGGTLPGSITVEPSENLQSLMEKNQSLVEENEKLSRGLSEAAGQTAQMLERIILTEQANEKMNAKLEELRQHAACKLDLQKLVETLEDQELKENVEIICNLQQLITQLSDETVACMAAAIDTAVEQEAQVETSPETSRSSDAFTTQHALRQAQMSKELVELNKALALKEALARKMTQNDSQLQPIQYQYQDNIKELELEVINLQKEKEELVLELQTAKKDANQAKLSERRRKRLQELEGQIADLKKKLNEQSKLLKLKESTERTVSKLNQEIRMMKNQRVQLMRQMKEDAEKFRQWKQKKDKEVIQLKERDRKRQYELLKLERNFQKQSNVLRRKTEEAAAANKRLKDALQKQREVADKRKETQSRGMEGTAARVKNWLGNEIEVMVSTEEAKRHLNDLLEDRKILAQDVAQLKEKKESGENPPPKLRRRTFSLTEVRGQVSESEDSITKQIESLETEMEFRSAQIADLQQKLLDAESEDRPKQRWENIATILEAKCALKYLIGELVSSKIQVSKLESSLKQSKTSCADMQKMLFEERNHFAEIETELQAELVRMEQQHQEKVLYLLSQLQQSQMAEKQLEESVSEKEQQLLSTLKCQDEELEKMREVCEQNQQLLRENEIIKQKLTLLQVASRQKHLPKDTLLSPDSSFEYVPPKPKPSRVKEKFLEQSMDIEDLKYCSEHSVNEHEDGDGDDDEGDDEEWKPTKLVKVSRKNIQGCSCKGWCGNKQCGCRKQKSDCGVDCCCDPTKCRNRQQGKDSLGTVERTQDSEGSFKLEDPTEVTPGLSFFNPVCATPNSKILKEMCDVEQVLSKKTPPAPSPFDLPELKHVATEYQENKAPGKKKKRALASNTSFFSGCSPIEEEAH AD1B7C2A10AB24DB o94880-1 PHD finger protein 14 PHF14 KIAA0783 protein protein human Homo sapiens Human MDRSSKRRQVKPLAASLLEALDYDSSDDSDFKVGDASDSEGSGNGSEDASKDSGEGSCSDSEENILEEELNEDIKVKEEQLKNSAEEEVLSSEKQLIKMEKKEEEENGERPRKKKEKEKEKEKEKEKEKEREKEKEKATVSENVAASAAATTPATSPPAVNTSPSVPTTTTATEEQVSEPKKWNLRRNRPLLDFVSMEELNDMDDYDSEDDNDWRPTVVKRKGRSASQKEGSDGDNEDDEDEGSGSDEDENDEGNDEDHSSPASEGGCKKKKSKVLSRNSADDEELTNDSLTLSQSKSNEDSLILEKSQNWSSQKMDHILICCVCLGDNSEDADEIIQCDNCGITVHEGCYGVDGESDSIMSSASENSTEPWFCDACKCGVSPSCELCPNQDGIFKETDAGRWVHIVCALYVPGVAFGDIDKLRPVTLTEMNYSKYGAKECSFCEDPRFARTGVCISCDAGMCRAYFHVTCAQKEGLLSEAAAEEDIADPFFAYCKQHADRLDRKWKRKNYLALQSYCKMSLQEREKQLSPEAQARINARLQQYRAKAELARSTRPQAWVPREKLPRPLTSSASAIRKLMRKAELMGISTDIFPVDNSDTSSSVDGRRKHKQPALTADFVNYYFERNMRMIQIQENMAEQKNIKDKLENEQEKLHVEYNKLCESLEELQNLNGKLRSEGQGIWALLGRITGQKLNIPAILRAPKERKPSKKEGGTQKTSTLPAVLYSCGICKKNHDQHLLLLCDTCKLHYHLGCLDPPLTRMPRKTKNSYWQCSECDQAGSSDMEADMAMETLPDGTKRSRRQIKEPVKFVPQDVPPEPKKIPIRNTRTRGRKRSFVPEEEKHEERVPRERRQRQSVLQKKPKAEDLRTECATCKGTGDNENLVRYPS DC356A84BE525932 phf14_human PHD finger protein 14 KIAA0783 PHF14 protein protein human Homo sapiens Human MDRSSKRRQVKPLAASLLEALDYDSSDDSDFKVGDASDSEGSGNGSEDASKDSGEGSCSDSEENILEEELNEDIKVKEEQLKNSAEEEVLSSEKQLIKMEKKEEEENGERPRKKKEKEKEKEKEKEKEKEREKEKEKATVSENVAASAAATTPATSPPAVNTSPSVPTTTTATEEQVSEPKKWNLRRNRPLLDFVSMEELNDMDDYDSEDDNDWRPTVVKRKGRSASQKEGSDGDNEDDEDEGSGSDEDENDEGNDEDHSSPASEGGCKKKKSKVLSRNSADDEELTNDSLTLSQSKSNEDSLILEKSQNWSSQKMDHILICCVCLGDNSEDADEIIQCDNCGITVHEGCYGVDGESDSIMSSASENSTEPWFCDACKCGVSPSCELCPNQDGIFKETDAGRWVHIVCALYVPGVAFGDIDKLRPVTLTEMNYSKYGAKECSFCEDPRFARTGVCISCDAGMCRAYFHVTCAQKEGLLSEAAAEEDIADPFFAYCKQHADRLDRKWKRKNYLALQSYCKMSLQEREKQLSPEAQARINARLQQYRAKAELARSTRPQAWVPREKLPRPLTSSASAIRKLMRKAELMGISTDIFPVDNSDTSSSVDGRRKHKQPALTADFVNYYFERNMRMIQIQENMAEQKNIKDKLENEQEKLHVEYNKLCESLEELQNLNGKLRSEGQGIWALLGRITGQKLNIPAILRAPKERKPSKKEGGTQKTSTLPAVLYSCGICKKNHDQHLLLLCDTCKLHYHLGCLDPPLTRMPRKTKNSYWQCSECDQAGSSDMEADMAMETLPDGTKRSRRQIKEPVKFVPQDVPPEPKKIPIRNTRTRGRKRSFVPEEEKHEERVPRERRQRQSVLQKKPKAEDLRTECATCKGTGDNENLVRYPS DC356A84BE525932 cenpe_human Centromere-associated protein E CENPE Kinesin-related protein CENPE Centromere protein E Kinesin-7 protein protein human Homo sapiens Human MAEEGAVAVCVRVRPLNSREESLGETAQVYWKTDNNVIYQVDGSKSFNFDRVFHGNETTKNVYEEIAAPIIDSAIQGYNGTIFAYGQTASGKTYTMMGSEDHLGVIPRAIHDIFQKIKKFPDREFLLRVSYMEIYNETITDLLCGTQKMKPLIIREDVNRNVYVADLTEEVVYTSEMALKWITKGEKSRHYGETKMNQRSSRSHTIFRMILESREKGEPSNCEGSVKVSHLNLVDLAGSERAAQTGAAGVRLKEGCNINRSLFILGQVIKKLSDGQVGGFINYRDSKLTRILQNSLGGNAKTRIICTITPVSFDETLTALQFASTAKYMKNTPYVNEVSTDEALLKRYRKEIMDLKKQLEEVSLETRAQAMEKDQLAQLLEEKDLLQKVQNEKIENLTRMLVTSSSLTLQQELKAKRKRRVTWCLGKINKMKNSNYADQFNIPTNITTKTHKLSINLLREIDESVCSESDVFSNTLDTLSEIEWNPATKLLNQENIESELNSLRADYDNLVLDYEQLRTEKEEMELKLKEKNDLDEFEALERKTKKDQEMQLIHEISNLKNLVKHAEVYNQDLENELSSKVELLREKEDQIKKLQEYIDSQKLENIKMDLSYSLESIEDPKQMKQTLFDAETVALDAKRESAFLRSENLELKEKMKELATTYKQMENDIQLYQSQLEAKKKMQVDLEKELQSAFNEITKLTSLIDGKVPKDLLCNLELEGKITDLQKELNKEVEENEALREEVILLSELKSLPSEVERLRKEIQDKSEELHIITSEKDKLFSEVVHKESRVQGLLEEIGKTKDDLATTQSNYKSTDQEFQNFKTLHMDFEQKYKMVLEENERMNQEIVNLSKEAQKFDSSLGALKTELSYKTQELQEKTREVQERLNEMEQLKEQLENRDSTLQTVEREKTLITEKLQQTLEEVKTLTQEKDDLKQLQESLQIERDQLKSDIHDTVNMNIDTQEQLRNALESLKQHQETINTLKSKISEEVSRNLHMEENTGETKDEFQQKMVGIDKKQDLEAKNTQTLTADVKDNEIIEQQRKIFSLIQEKNELQQMLESVIAEKEQLKTDLKENIEMTIENQEELRLLGDELKKQQEIVAQEKNHAIKKEGELSRTCDRLAEVEEKLKEKSQQLQEKQQQLLNVQEEMSEMQKKINEIENLKNELKNKELTLEHMETERLELAQKLNENYEEVKSITKERKVLKELQKSFETERDHLRGYIREIEATGLQTKEELKIAHIHLKEHQETIDELRRSVSEKTAQIINTQDLEKSHTKLQEEIPVLHEEQELLPNVKEVSETQETMNELELLTEQSTTKDSTTLARIEMERLRLNEKFQESQEEIKSLTKERDNLKTIKEALEVKHDQLKEHIRETLAKIQESQSKQEQSLNMKEKDNETTKIVSEMEQFKPKDSALLRIEIEMLGLSKRLQESHDEMKSVAKEKDDLQRLQEVLQSESDQLKENIKEIVAKHLETEEELKVAHCCLKEQEETINELRVNLSEKETEISTIQKQLEAINDKLQNKIQEIYEKEEQFNIKQISEVQEKVNELKQFKEHRKAKDSALQSIESKMLELTNRLQESQEEIQIMIKEKEEMKRVQEALQIERDQLKENTKEIVAKMKESQEKEYQFLKMTAVNETQEKMCEIEHLKEQFETQKLNLENIETENIRLTQILHENLEEMRSVTKERDDLRSVEETLKVERDQLKENLRETITRDLEKQEELKIVHMHLKEHQETIDKLRGIVSEKTNEISNMQKDLEHSNDALKAQDLKIQEELRIAHMHLKEQQETIDKLRGIVSEKTDKLSNMQKDLENSNAKLQEKIQELKANEHQLITLKKDVNETQKKVSEMEQLKKQIKDQSLTLSKLEIENLNLAQKLHENLEEMKSVMKERDNLRRVEETLKLERDQLKESLQETKARDLEIQQELKTARMLSKEHKETVDKLREKISEKTIQISDIQKDLDKSKDELQKKIQELQKKELQLLRVKEDVNMSHKKINEMEQLKKQFEAQNLSMQSVRMDNFQLTKKLHESLEEIRIVAKERDELRRIKESLKMERDQFIATLREMIARDRQNHQVKPEKRLLSDGQQHLTESLREKCSRIKELLKRYSEMDDHYECLNRLSLDLEKEIEFQKELSMRVKANLSLPYLQTKHIEKLFTANQRCSMEFHRIMKKLKYVLSYVTKIKEEQHESINKFEMDFIDEVEKQKELLIKIQHLQQDCDVPSRELRDLKLNQNMDLHIEEILKDFSESEFPSIKTEFQQVLSNRKEMTQFLEEWLNTRFDIEKLKNGIQKENDRICQVNNFFNNRIIAIMNESTEFEERSATISKEWEQDLKSLKEKNEKLFKNYQTLKTSLASGAQVNPTTQDNKNPHVTSRATQLTTEKIRELENSLHEAKESAMHKESKIIKMQKELEVTNDIIAKLQAKVHESNKCLEKTKETIQVLQDKVALGAKPYKEEIEDLKMKLVKIDLEKMKNAKEFEKEISATKATVEYQKEVIRLLRENLRRSQQAQDTSVISEHTDPQPSNKPLTCGGGSGIVQNTKALILKSEHIRLEKEISKLKQQNEQLIKQKNELLSNNQHLSNEVKTWKERTLKREAHKQVTCENSPKSPKVTGTASKKKQITPSQCKERNLQDPVPKESPKSCFFDSRSKSLPSPHPVRYFDNSSLGLCPEVQNAGAESVDSQPGPWHASSGKDVPECKTQ DIP protein Q02224 original sequence version: 157 4BC59C2EF0B02D88 ccnd1-cenpe-1 5 8 unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role neutral component neutral component subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level association association Figure 4A KIF4A, CENPE and cyclinD1 were clearly co-fractionated with PHF14. KIF4A, CENPE and cyclinD1 were clearly co-fractionated with PHF14. kif4a-phf14-1 1 9 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Figure 4B kif4a-phf14-2 6 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n_terminal_region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 4C RHS kif4a-phf14-3 1 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Figure 4Bi kif4a-phf14-4 4 10 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n_terminal_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 4C LHS phf14-kif4a-1 2 11 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range stalk_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 4D I assume that PHF14 has no tag as I cannot see any mention of one Binding regions were between the N-terminal region of PHF14 1-160 a.a which contains a coiled-coil domain, and the stalk domain of KIF4A (middle region). 578f7tutgy-1 3 11 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell 9 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization Figure 4E PHF14 and KIF4A were co-localized not only in the nuclei in interphase cells but also dynamically on the condensed chromatin in mitotic cells at different stages PHF14 and KIF4A were co-localized not only in the nuclei in interphase cells but also dynamically on the condensed chromatin in mitotic cells at different stages. I cannot tell from the images which stages of mitosis are meant to be positive - so no GO terms entered other than "interphase". sr6uhfgh-1 7 9 unspecified role unspecified role prey prey cell lysate cell lysate n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 10 unspecified role unspecified role bait bait purified purified n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminal_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Supplementary figure 4B a faint band was visible.