IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structural, mutational and biophysical studies reveal a canonical mode of molecular recognition between immune receptor TIGIT and nectin-2. Structural, mutational and biophysical studies reveal a canonical mode of molecular recognition between immune receptor TIGIT and nectin-2. Molecular immunology 2017 imex curation Samanta D., Guo H., Rubinstein R., Ramagopal UA., Almo SC. steve.almo@einstein.yu.edu 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Samanta D., Guo H., Rubinstein R., Ramagopal UA., Almo SC. steve.almo@einstein.yu.edu Accepted 2017-OCT-04 AT 15:13 BST AT 15:13 BST by ORCHARD Molecular immunology 2017 imex curation Structural, mutational and biophysical studies reveal a canonical mode of molecular recognition between immune receptor TIGIT and nectin-2. Structural, mutational and biophysical studies reveal a canonical mode of molecular recognition between immune receptor TIGIT and nectin-2. Molecular immunology 2017 imex curation Samanta D., Guo H., Rubinstein R., Ramagopal UA., Almo SC. steve.almo@einstein.yu.edu 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Samanta D., Guo H., Rubinstein R., Ramagopal UA., Almo SC. steve.almo@einstein.yu.edu Accepted 2017-OCT-04 AT 15:13 BST AT 15:13 BST by ORCHARD Molecular immunology 2017 imex curation Structural, mutational and biophysical studies reveal a canonical mode of molecular recognition between immune receptor TIGIT and nectin-2. Structural, mutational and biophysical studies reveal a canonical mode of molecular recognition between immune receptor TIGIT and nectin-2. Molecular immunology 2017 imex curation Samanta D., Guo H., Rubinstein R., Ramagopal UA., Almo SC. steve.almo@einstein.yu.edu 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Samanta D., Guo H., Rubinstein R., Ramagopal UA., Almo SC. steve.almo@einstein.yu.edu Accepted 2017-OCT-04 AT 15:13 BST AT 15:13 BST by ORCHARD Molecular immunology 2017 imex curation nect2_human Nectin-2 NECTIN2 HVEB Herpes virus entry mediator B Nectin cell adhesion molecule 2 PRR2 PVRL2 Poliovirus receptor-related protein 2 protein protein human Homo sapiens Human MARAAALLPSRSPPTPLLWPLLLLLLLETGAQDVRVQVLPEVRGQLGGTVELPCHLLPPVPGLYISLVTWQRPDAPANHQNVAAFHPKMGPSFPSPKPGSERLSFVSAKQSTGQDTEAELQDATLALHGLTVEDEGNYTCEFATFPKGSVRGMTWLRVIAKPKNQAEAQKVTFSQDPTTVALCISKEGRPPARISWLSSLDWEAKETQVSGTLAGTVTVTSRFTLVPSGRADGVTVTCKVEHESFEEPALIPVTLSVRYPPEVSISGYDDNWYLGRTDATLSCDVRSNPEPTGYDWSTTSGTFPTSAVAQGSQLVIHAVDSLFNTTFVCTVTNAVGMGRAEQVIFVRETPNTAGAGATGGIIGGIIAAIIATAVAATGILICRQQRKEQTLQGAEEDEDLEGPPSYKPPTPKAKLEAQEMPSQLFTLGASEHSPLKTPYFDAGASCTEQEMPRYHELPTLEERSGPLHPGATSLGSPIPVPPGPPAVEDVSLDLEDEEGEEEEEYLDKINPIYDALSYSSPSDSYQGKGFVMSRAMYV DIP protein Q92692 original sequence version: 131 DIP protein Q92692 original sequence version: 134 3AE4F83E92F6F624 tigit_human T-cell immunoreceptor with Ig and ITIM domains TIGIT VSIG9 VSTM3 V-set and immunoglobulin domain-containing protein 9 V-set and transmembrane domain-containing protein 3 protein protein human Homo sapiens Human MRWCLLLIWAQGLRQAPLASGMMTGTIETTGNISAEKGGSIILQCHLSSTTAQVTQVNWEQQDQLLAICNADLGWHISPSFKDRVAPGPGLGLTLQSLTVNDTGEYFCIYHTYPDGTYTGRIFLEVLESSVAEHGARFQIPLLGAMAATLVVICTAVIVVVALTRKKKALRIHSVEGDLRRKSAGQEEWSPSAPSPPGSCVQAEAAPAGLCGEQRGEDCAELHDYFNVLSYRSLGNCSFFTETG B890AF73F618C851 p15151-1 Isoform Alpha of Poliovirus receptor PVR PVS protein protein human Homo sapiens Human MARAMAAAWPLLLVALLVLSWPPPGTGDVVVQAPTQVPGFLGDSVTLPCYLQVPNMEVTHVSQLTWARHGESGSMAVFHQTQGPSYSESKRLEFVAARLGAELRNASLRMFGLRVEDEGNYTCLFVTFPQGSRSVDIWLRVLAKPQNTAEVQKVQLTGEPVPMARCVSTGGRPPAQITWHSDLGGMPNTSQVPGFLSGTVTVTSLWILVPSSQVDGKNVTCKVEHESFEKPQLLTVNLTVYYPPEVSISGYDNNWYLGQNEATLTCDARSNPEPTGYNWSTTMGPLPPFAVAQGAQLLIRPVDKPINTTLICNVTNALGARQAELTVQVKEGPPSEHSGISRNAIIFLVLGILVFLILLGIGIYFYWSKCSREVLWHCHLCPSSTEHASASANGHVSYSAVSRENSSSQDPQTEGTR D15C012CE853169B tigit-3 1 5 unspecified role unspecified role neutral component neutral component first_Ig_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1 2.7A R Work =0.206 R Free = 0.253 tigit-1 2 5 unspecified role unspecified role neutral component neutral component first_Ig_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 1C the IgV domain of human TIGIT is predominantly a monomer at low concentrations in solution, with the propensity to form a dimer at higher concentration. Detailed analysis on the gel filtration profile revealed that TIGIT can form a dimer in solution nectin2-tigit-6 3 4 unspecified role unspecified role bait bait first_Ig_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey first_Ig_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q495A1:p.Thr112Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T A Wed May 02 14:26:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr112ala' Tue Jun 30 16:12:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr112Ala' Q495A1:p.Tyr113Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 15:17:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr113ala' Tue Jun 30 20:02:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr113Ala' Q495A1:p.Pro114Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain P A Wed May 02 16:45:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro114ala' Tue Jun 30 18:01:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro114Ala' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 4A, 5D nectin2-tigit-7 3 6 unspecified role unspecified role bait bait ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range mouse-nso NSO-mouse myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc 5 unspecified role unspecified role prey prey first_Ig_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 4B P15151-1 isoform would not add- Noe fixing