MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url&gt;http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Sinomenine activates astrocytic dopamine D2 receptors and alleviates neuroinflammatory injury via the CRYAB/STAT3 pathway after ischemic stroke in mice. Sinomenine activates astrocytic dopamine D2 receptors and alleviates neuroinflammatory injury via the CRYAB/STAT3 pathway after ischemic stroke in mice. Journal of neuroinflammation 2016 imex curation Qiu J., Yan Z., Tao K., Li Y., Li Y., Li J., Dong Y., Feng D., Chen H. dayunfmmu@163.com,chsshenyang@sina.cn 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-astrocyte Mus musculus astrocyte [Brain astrocytes; Astroglia] astrocyte Astrocytes [Brain astrocytes; Astroglia] fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Qiu J., Yan Z., Tao K., Li Y., Li Y., Li J., Dong Y., Feng D., Chen H. dayunfmmu@163.com,chsshenyang@sina.cn dayunfmmu@163.com;chsshenyang@sina.cn Accepted 2017-NOV-21 AT 14:10 GMT AT 14:10 GMT by LUANA Journal of neuroinflammation 2016 Only protein-protein interactions imex curation imex curation Sinomenine activates astrocytic dopamine D2 receptors and alleviates neuroinflammatory injury via the CRYAB/STAT3 pathway after ischemic stroke in mice. Sinomenine activates astrocytic dopamine D2 receptors and alleviates neuroinflammatory injury via the CRYAB/STAT3 pathway after ischemic stroke in mice. Journal of neuroinflammation 2016 imex curation Qiu J., Yan Z., Tao K., Li Y., Li Y., Li J., Dong Y., Feng D., Chen H. dayunfmmu@163.com,chsshenyang@sina.cn 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-astrocyte Mus musculus astrocyte [Brain astrocytes; Astroglia] astrocyte Astrocytes [Brain astrocytes; Astroglia] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Qiu J., Yan Z., Tao K., Li Y., Li Y., Li J., Dong Y., Feng D., Chen H. dayunfmmu@163.com,chsshenyang@sina.cn dayunfmmu@163.com;chsshenyang@sina.cn Accepted 2017-NOV-21 AT 14:10 GMT AT 14:10 GMT by LUANA Journal of neuroinflammation 2016 Only protein-protein interactions imex curation imex curation stat3_mouse Signal transducer and activator of transcription 3 Acute-phase response factor Stat3 Aprf protein protein mouse Mus musculus Mouse MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMDLTSECATSPM DIP protein P42227 original sequence version: 169 DIP protein P42227 original sequence version: 162 DIP protein P42227 original sequence version: 164 DIP protein P42227 original sequence version: 141 6C00626711C8012D cryab_mouse Alpha-crystallin B chain Cryab Crya2 Alpha(B)-crystallin P23 protein protein mouse Mus musculus Mouse MDIAIHHPWIRRPFFPFHSPSRLFDQFFGEHLLESDLFSTATSLSPFYLRPPSFLRAPSWIDTGLSEMRLEKDRFSVNLDVKHFSPEELKVKVLGDVIEVHGKHEERQDEHGFISREFHRKYRIPADVDPLTITSSLSSDGVLTVNGPRKQVSGPERTIPITREEKPAVAAAPKK DIP protein P23927 original sequence version: 94 46702976C008EFD3 cryab-stat3-1 1 3 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell phosTyr705 phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm 4 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization f6b Interestingly, Sino significantly increased the nuclear expression of CRYAB and inhibited the phosphorylation of STAT3 after OGD in primary astrocytes, with the co-localization of CRYAB and p-STAT3 in the nuclei (Fig. 6b, ​,c).c). cryab-stat3-2 2 3 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level phoTyr705 phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) certain certain sequence position certain certain certain sequence position certain observed-ptm observed-ptm observed ptm observed-ptm 4 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f7c Co-IP (Fig. 7c–e) showed that Sino enhanced the interaction between CRYAB and STAT3, with increased expression of CRYAB in the immunocomplex after OGD (p < 0.01, Sino group vs. control group).