DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization. Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization. Nature 2016 imex curation Ohto U., Ishida H., Krayukhina E., Uchiyama S., Inoue N., Shimizu T. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Ohto U., Ishida H., Krayukhina E., Uchiyama S., Inoue N., Shimizu T. DIP original experiment: DIP-269107X Nature 2016 IMEx Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization. Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization. Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization. Nature 2016 imex curation Ohto U., Ishida H., Krayukhina E., Uchiyama S., Inoue N., Shimizu T. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Ohto U., Ishida H., Krayukhina E., Uchiyama S., Inoue N., Shimizu T. DIP original experiment: DIP-269113X Nature 2016 IMEx Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization. Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization. Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization. Nature 2016 imex curation Ohto U., Ishida H., Krayukhina E., Uchiyama S., Inoue N., Shimizu T. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Ohto U., Ishida H., Krayukhina E., Uchiyama S., Inoue N., Shimizu T. DIP original experiment: DIP-269109X Nature 2016 IMEx Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization. Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization. Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization. Nature 2016 imex curation Ohto U., Ishida H., Krayukhina E., Uchiyama S., Inoue N., Shimizu T. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ohto U., Ishida H., Krayukhina E., Uchiyama S., Inoue N., Shimizu T. DIP original experiment: DIP-269111X Nature 2016 IMEx Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization. Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization. Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization. Nature 2016 imex curation Ohto U., Ishida H., Krayukhina E., Uchiyama S., Inoue N., Shimizu T. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Ohto U., Ishida H., Krayukhina E., Uchiyama S., Inoue N., Shimizu T. DIP original experiment: DIP-269108X Nature 2016 IMEx Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization. Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization. Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization. Nature 2016 imex curation Ohto U., Ishida H., Krayukhina E., Uchiyama S., Inoue N., Shimizu T. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ohto U., Ishida H., Krayukhina E., Uchiyama S., Inoue N., Shimizu T. DIP original experiment: DIP-269112X Nature 2016 IMEx Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization. Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization. Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization. Nature 2016 imex curation Ohto U., Ishida H., Krayukhina E., Uchiyama S., Inoue N., Shimizu T. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Ohto U., Ishida H., Krayukhina E., Uchiyama S., Inoue N., Shimizu T. DIP original experiment: DIP-269110X Nature 2016 IMEx Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization. a6nd01-1 Sperm-egg fusion protein Juno Folate receptor 4 Folate receptor delta IZUMO1 receptor protein JUNO IZUMO1R FOLR4 JUNO protein protein human Homo sapiens Human MACWWPLLLELWTVMPTWAGDELLNICMNAKHHKRVPSPEDKLYEECIPWKDNACCTLTTSWEAHLDVSPLYNFSLFHCGLLMPGCRKHFIQAICFYECSPNLGPWIQPVGSLGWEVAPSGQGERVVNVPLCQEDCEEWWEDCRMSYTCKSNWRGGWDWSQGKNRCPKGAQCLPFSHYFPTPADLCEKTWSNSFKASPERRNSGRCLQKWFEPAQGNPNVAVARLFASSAPSWELSYTIMVCSLFLPFLS DIP protein A6ND01-1 original sequence version: 65 No experimental confirmation available. Gene prediction based on EST data. q8iyv9-1 Izumo sperm-egg fusion protein 1 Oocyte binding/fusion factor Sperm-specific protein izumo IZUMO1 protein protein human Homo sapiens Human MGPHFTLLCAALAGCLLPAEGCVICDPSVVLALKSLEKDYLPGHLDAKHHKAMMERVENAVKDFQELSLNEDAYMGVVDEATLQKGSWSLLKDLKRITDSDVKGDLFVKELFWMLHLQKETFATYVARFQKEAYCPNKCGVMLQTLIWCKNCKKEVHACRKSYDCGERNVEVPQMEDMILDCELNWHQASEGLTDYSFYRVWGNNTETLVSKGKEATLTKPMVGPEDAGSYRCELGSVNSSPATIINFHVTVLPKMIKEEKPSPNIVTPGEATTESSISLQPLQPEKMLASRLLGLLICGSLALITGLTFAIFRRRKVIDFIKSSLFGLGSGAAEQTQVPKEKATDSRQQ DIP protein Q8IYV9-1 original sequence version: 114 izumo1-izumo1r-1 3 8 unspecified role unspecified role unspecified role unspecified role Region 20-228 Region 20-228 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 9 unspecified role unspecified role unspecified role unspecified role Region 22-255 Region 22-255 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q8IYV9-1:p.Trp148Ala W148A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W148A'. Wed May 02 14:53:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp148ala' Tue Jun 30 19:29:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp148Ala' drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction izumo1-1 2 9 unspecified role unspecified role unspecified role unspecified role Region 22-255 Region 22-255 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction izumo1-izumo1r-2 1 8 unspecified role unspecified role unspecified role unspecified role Region 20-228 Region 20-228 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 9 unspecified role unspecified role unspecified role unspecified role Region 22-255 Region 22-255 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction izumo1-izumo1r-3 4 8 unspecified role unspecified role unspecified role unspecified role Region 20-228 Region 20-228 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 9 unspecified role unspecified role unspecified role unspecified role Region 22-255 Region 22-255 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction izumo1-izumo1r-4 5 8 unspecified role unspecified role unspecified role unspecified role Region 20-228 Region 20-228 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 9 unspecified role unspecified role unspecified role unspecified role Region 22-255 Region 22-255 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction izumo1-izumo1r-5 7 8 unspecified role unspecified role unspecified role unspecified role Region 20-228 Region 20-228 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain A6ND01-1:p.Trp62Ala W62A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W62A'. Wed May 02 16:33:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp62ala' Tue Jun 30 19:10:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp62Ala' A6ND01-1:p.Leu81Ala L81A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L81A'. Wed May 02 15:00:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu81ala' Tue Jun 30 20:11:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu81Ala' A6ND01-1:p.Met83Ala M83A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain M A This feature has been corrected as a result of our quality control procedures. The original label was 'M83A'. Wed May 02 15:50:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met83ala' Tue Jun 30 17:51:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met83Ala' drome Drosophila melanogaster (Fruit fly) Fruit fly 9 unspecified role unspecified role unspecified role unspecified role Region 22-255 Region 22-255 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction izumo1-2 6 9 unspecified role unspecified role unspecified role unspecified role Region 22-255 Region 22-255 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction