DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis of transcription activation. Structural basis of transcription activation. Science (New York, N.Y.) 2016 imex curation Feng Y., Zhang Y., Ebright RH. Only protein-protein interactions in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Feng Y., Zhang Y., Ebright RH. DIP original experiment: DIP-269142X Science (New York, N.Y.) 2016 IMEx Structural basis of transcription activation. Structural basis of transcription activation. Structural basis of transcription activation. Science (New York, N.Y.) 2016 imex curation Feng Y., Zhang Y., Ebright RH. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Feng Y., Zhang Y., Ebright RH. DIP original experiment: DIP-269141X Science (New York, N.Y.) 2016 IMEx Structural basis of transcription activation. rpoa_thet8 DNA-directed RNA polymerase subunit alpha Transcriptase subunit alpha RNA polymerase subunit alpha TTHA1664 rpoA protein protein thet8 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) MLDSKLKAPVFTVRTQGREYGEFVLEPLERGFGVTLGNPLRRILLSSIPGTAVTSVYIEDVLHEFSTIPGVKEDVVEIILNLKELVVRFLNPSLQTVTLLLKAEGPKEVKARDFLPVADVEIMNPDLHIATLEEGGRLNMEVRVDRGVGYVPAEKHGIKDRINAIPVDAVFSPVRRVAFQVEDTRLGQRTDLDKLTLRIWTDGSVTPLEALNQAVEILREHLTYFSNPQAAAVAAPEEAKEPEAPPEQEEELDLPLEELGLSTRVLHSLKEEGIESVRALLALNLKDLKNIPGIGERSLEEIKEALEKKGFTLKE DIP protein Q5SHR6 original sequence version: 69 DIP protein Q5SHR6 original sequence version: 49 DIP protein Q5SHR6 original sequence version: 85 DIP protein Q5SHR6 original sequence version: 83 F79D93B57526A1CB rpob_thet8 DNA-directed RNA polymerase subunit beta Transcriptase subunit beta RNA polymerase subunit beta TTHA1813 rpoB protein protein thet8 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) MEIKRFGRIREVIPLPPLTEIQVESYRRALQADVPPEKRENVGIQAAFRETFPIEEEDKGKGGLVLDFLEYRLGEPPFPQDECREKDLTYQAPLYARLQLIHKDTGLIKEDEVFLGHIPLMTEDGSFIINGADRVIVSQIHRSPGVYFTPDPARPGRYIASIIPLPKRGPWIDLEVEPNGVVSMKVNKRKFPLVLLLRVLGYDQETLARELGAYGELVQGLMDESVFAMRPEEALIRLFTLLRPGDPPKRDKAVAYVYGLIADPRRYDLGEAGRYKAEEKLGIRLSGRTLARFEDGEFKDEVFLPTLRYLFALTAGVPGHEVDDIDHLGNRRIRTVGELMTDQFRVGLARLARGVRERMLMGSEDSLTPAKLVNSRPLEAAIREFFSRSQLSQFKDETNPLSSLRHKRRISALGPGGLTRERAGFDVRDVHRTHYGRICPVETPEGANIGLITSLAAYARVDELGFIRTPYRRVVGGVVTDEVVYMTATEEDRYTIAQANTPLEGNRIAAERVVARRKGEPVIVSPEEVEFMDVSPKQVFSVNTNLIPFLEHDDANRALMGSNMQTQAVPLIRAQAPVVMTGLEERVVRDSLAALYAEEDGEVAKVDGNRIVVRYEDGRLVEYPLRRFYRSNQGTALDQRPRVVVGQRVRKGDLLADGPASENGFLALGQNVLVAIMPFDGYNFEDAIVISEELLKRDFYTSIHIERYEIEARDTKLGPERITRDIPHLSEAALRDLDEEGVVRIGAEVKPGDILVGRTSFKGESEPTPEERLLRSIFGEKARDVKDTSLRVPPGEGGIVVRTVRLRRGDPGVELKPGVREVVRVYVAQKRKLQVGDKLANRHGNKGVVAKILPVEDMPHLPDGTPVDVILNPLGVPSRMNLGQILETHLGLAGYFLGQRYISPIFDGAKEPEIKELLAQAFEVYFGKRKGEGFGVDKREVEVLRRAEKLGLVTPGKTPEEQLKELFLQGKVVLYDGRTGEPIEGPIVVGQMFIMKLYHMVEDKMHARSTGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGAAHTLQEMLTLKSDDIEGRNAAYEAIIKGEDVPEPSVPESFRVLVKELQALALDVQTLDEKDNPVDIFEGLASKR DIP protein Q8RQE9 original sequence version: 83 DIP protein Q8RQE9 original sequence version: 63 DIP protein Q8RQE9 original sequence version: 102 DIP protein Q8RQE9 original sequence version: 100 C1260F31CEC6B40D rpoc_thet8 DNA-directed RNA polymerase subunit beta' rpoC Transcriptase subunit beta' RNA polymerase subunit beta' TTHA1812 protein protein thet8 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) MKKEVRKVRIALASPEKIRSWSYGEVEKPETINYRTLKPERDGLFDERIFGPIKDYECACGKYKRQRFEGKVCERCGVEVTKSIVRRYRMGHIELATPAAHIWFVKDVPSKIGTLLDLSATELEQVLYFSKYIVLDPKGAILNGVPVEKRQLLTDEEYRELRYGKQETYPLPPGVDALVKDGEEVVKGQELAPGVVSRLDGVALYRFPRRVRVEYVKKERAGLRLPLAAWVEKEAYKPGEILAELPEPYLFRAEEEGVVELKELEEGAFLVLRREDEPVATYFLPVGMTPLVVHGEIVEKGQPLAEAKGLLRMPRQVRAAQVEAEEEGETVYLTLFLEWTEPKDYRVQPHMNVVVPEGARVEAGDKIVAAIDPEEEVIAEAEGVVHLHEPASILVVKARVYPFEDDVEVSTGDRVAPGDVLADGGKVKSDVYGRVEVDLVRNVVRVVESYDIDARMGAEAIQQLLKELDLEALEKELLEEMKHPSRARRAKARKRLEVVRAFLDSGNRPEWMILEAVPVLPPDLRPMVQVDGGRFATSDLNDLYRRLINRNNRLKKLLAQGAPEIIIRNEKRMLQEAVDALLDNGRRGAPVTNPGSDRPLRSLTDILSGKQGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKRMALELFKPFLLKKMEEKGIAPNVKAARRMLERQRDIKDEVWDALEEVIHGKVVLLNRAPTLHRLGIQAFQPVLVEGQSIQLHPLVCEAFNADFDGDQMAVHVPLSSFAQAEARIQMLSAHNLLSPASGEPLAKPSRDIILGLYYITQVRKEKKGAGLEFATPEEALAAHERGEVALNAPIKVAGRETSVGRLKYVFANPDEALLAVAHGIVDLQDVVTVRYMGKRLETSPGRILFARIVAEAVEDEKVAWELIQLDVPQEKNSLKDLVYQAFLRLGMEKTARLLDALKYYGFTFSTTSGITIGIDDAVIPEEKKQYLEEADRKLLQIEQAYEMGFLTDRERYDQILQLWTETTEKVTQAVFKNFEENYPFNPLYVMAQSGARGNPQQIRQLCGLRGLMQKPSGETFEVPVRSSFREGLTVLEYFISSHGARKGGADTALRTADSGYLTRKLVDVTHEIVVREADCGTTNYISVPLFQPDEVTRSLRLRKRADIEAGLYGRVLAREVEVLGVRLEEGRYLSMDDVHLLIKAAEAGEIQEVPVRSPLTCQTRYGVCQKCYGYDLSMARPVSIGEAVGIVAAQSIGEPGTQLTMRTFHTGGVAGAADITQGLPRVIELFEARRPKAKAVISEIDGVVRIEETEEKLSVFVESEGFSKEYKLPKEARLLVKDGDYVEAGQPLTRGAIDPHQLLEAKGPEAVERYLVEEIQKVYRAQGVKLHDKHIEIVVRQMMKYVEVTDPGDSRLLEGQVLEKWDVEALNERLIAEGKTPVAWKPLLMGVTKSALSTKSWLSAASFQNTTHVLTEAAIAGKKDELIGLKENVILGRLIPAGTGSDFVRFTQVVDQKTLKAIEEARKEAVEAKERPAARRGVKREQPGKQA DIP protein Q8RQE8 original sequence version: 86 DIP protein Q8RQE8 original sequence version: 66 DIP protein Q8RQE8 original sequence version: 104 DIP protein Q8RQE8 original sequence version: 102 A7B335945B08E3A7 rpoz_thet8 DNA-directed RNA polymerase subunit omega rpoZ Transcriptase subunit omega RNA polymerase omega subunit TTHA1561 protein protein thet8 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) MAEPGIDKLFGMVDSKYRLTVVVAKRAQQLLRHGFKNTVLEPEERPKMQTLEGLFDDPNAVTWAMKELLTGRLVFGENLVPEDRLQKEMERLYPVEREE DIP protein Q8RQE7 original sequence version: 81 DIP protein Q8RQE7 original sequence version: 62 DIP protein Q8RQE7 original sequence version: 97 DIP protein Q8RQE7 original sequence version: 96 1D213933B0B98965 ldrp_thet8 Transcriptional regulator LdrP Cyclic AMP receptor protein/Fumarate and nitrate reduction regulator superfamily protein LdrP Light induced transcription regulator-dependent regulatory protein LitR-dependent regulatory protein TTHB099 protein protein thet8 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) MKRFARKETIYLRGEEARTLYRLEEGLVRVVELLPDGRLITLRHVLPGDYFGEEALEGKAYRYTAEAMTEAVVQGLEPRAMDHEALHRVARNLARQMRRVQAYEAHLQTGELRARIARYLLFLADTPLSARDRQGIYVTVSHEEIADATASIRESVSKVLADLRREGLIATAYRRVYLLDLAALEREAGSALEAA DIP protein Q53W63 original sequence version: 90 q5skw1_thet8 RNA polymerase sigma factor SigA sigA TTHA0532 protein protein thet8 Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) MKKSKRKNAQAQEAQETEVLVQEEAEELPEFPEGEPDPDLEDPDLTLEDDLLDLPEEGEGLDLEEEEEDLPIPKISTSDPVRQYLHEIGQVPLLTLEEEVELARKVEEGMEAIKKLSEITGLDPDLIREVVRAKILGSARVRHIPGLKETLDPKTVEEIDQKLKSLPKEHKRYLHIAREGEAARQHLIEANLRLVVSIAKKYTGRGLSFLDLIQEGNQGLIRAVEKFEYKRRFKFSTYATWWIRQAINRAIADQARTIRIPVHMVETINKLSRTARQLQQELGREPTYEEIAEAMGPGWDAKRVEETLKIAQEPVSLETPIGDEKDSFYGDFIPDEHLPSPVDAATQSLLSEELEKALSKLSEREAMVLKLRKGLIDGREHTLEEVGAFFGVTRERIRQIENKALRKLKYHESRTRKLRDFLD DIP protein Q5SKW1 original sequence version: 67 DIP protein Q5SKW1 original sequence version: 92 DIP protein Q5SKW1 original sequence version: 88 D3A80BF9C929BD09 ldrp_thet8-rpoa-1 2 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 3 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association ldrp_thet8-rpoa-2 1 7 unspecified role unspecified role unspecified role unspecified role Q53W63:p.Asp125Lys D125K mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain D K This feature has been corrected as a result of our quality control procedures. The original label was 'D125K'. Wed May 02 15:47:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp125lys' Tue Jun 30 15:45:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp125Lys' Q53W63:p.Glu185Lys E185K mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E K This feature has been corrected as a result of our quality control procedures. The original label was 'E185K'. Wed May 02 16:27:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu185lys' Tue Jun 30 20:11:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu185Lys' ecolx Escherichia coli 3 unspecified role unspecified role unspecified role unspecified role Region 253-310 Region 253-310 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Fluorescein-label Fluorescein-label fluorescein fluorescein label fluorescein c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli direct interaction direct interaction