DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Crystal structure of the human sterol transporter ABCG5/ABCG8. Crystal structure of the human sterol transporter ABCG5/ABCG8. Nature 2016 imex curation Lee JY., Kinch LN., Borek DM., Wang J., Wang J., Urbatsch IL., Xie XS., Grishin NV., Cohen JC., Otwinowski Z., Hobbs HH., Rosenbaum DM. Only protein-protein interactions picpa Pichia pastoris (Yeast) Yeast Pichia pastoris pull down pull down predetermined predetermined participant predetermined Lee JY., Kinch LN., Borek DM., Wang J., Wang J., Urbatsch IL., Xie XS., Grishin NV., Cohen JC., Otwinowski Z., Hobbs HH., Rosenbaum DM. DIP original experiment: DIP-268790X Nature 2016 IMEx Crystal structure of the human sterol transporter ABCG5/ABCG8. Crystal structure of the human sterol transporter ABCG5/ABCG8. Crystal structure of the human sterol transporter ABCG5/ABCG8. Nature 2016 imex curation Lee JY., Kinch LN., Borek DM., Wang J., Wang J., Urbatsch IL., Xie XS., Grishin NV., Cohen JC., Otwinowski Z., Hobbs HH., Rosenbaum DM. Only protein-protein interactions picpa Pichia pastoris (Yeast) Yeast Pichia pastoris x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lee JY., Kinch LN., Borek DM., Wang J., Wang J., Urbatsch IL., Xie XS., Grishin NV., Cohen JC., Otwinowski Z., Hobbs HH., Rosenbaum DM. DIP original experiment: DIP-268788X Nature 2016 IMEx Crystal structure of the human sterol transporter ABCG5/ABCG8. Crystal structure of the human sterol transporter ABCG5/ABCG8. Crystal structure of the human sterol transporter ABCG5/ABCG8. Nature 2016 imex curation Lee JY., Kinch LN., Borek DM., Wang J., Wang J., Urbatsch IL., Xie XS., Grishin NV., Cohen JC., Otwinowski Z., Hobbs HH., Rosenbaum DM. Only protein-protein interactions picpa Pichia pastoris (Yeast) Yeast Pichia pastoris pull down pull down predetermined predetermined participant predetermined Lee JY., Kinch LN., Borek DM., Wang J., Wang J., Urbatsch IL., Xie XS., Grishin NV., Cohen JC., Otwinowski Z., Hobbs HH., Rosenbaum DM. DIP original experiment: DIP-268791X Nature 2016 IMEx Crystal structure of the human sterol transporter ABCG5/ABCG8. Crystal structure of the human sterol transporter ABCG5/ABCG8. Crystal structure of the human sterol transporter ABCG5/ABCG8. Nature 2016 imex curation Lee JY., Kinch LN., Borek DM., Wang J., Wang J., Urbatsch IL., Xie XS., Grishin NV., Cohen JC., Otwinowski Z., Hobbs HH., Rosenbaum DM. Only protein-protein interactions picpa Pichia pastoris (Yeast) Yeast Pichia pastoris comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Lee JY., Kinch LN., Borek DM., Wang J., Wang J., Urbatsch IL., Xie XS., Grishin NV., Cohen JC., Otwinowski Z., Hobbs HH., Rosenbaum DM. DIP original experiment: DIP-268792X Nature 2016 IMEx Crystal structure of the human sterol transporter ABCG5/ABCG8. Crystal structure of the human sterol transporter ABCG5/ABCG8. Crystal structure of the human sterol transporter ABCG5/ABCG8. Nature 2016 imex curation Lee JY., Kinch LN., Borek DM., Wang J., Wang J., Urbatsch IL., Xie XS., Grishin NV., Cohen JC., Otwinowski Z., Hobbs HH., Rosenbaum DM. Only protein-protein interactions picpa Pichia pastoris (Yeast) Yeast Pichia pastoris molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Lee JY., Kinch LN., Borek DM., Wang J., Wang J., Urbatsch IL., Xie XS., Grishin NV., Cohen JC., Otwinowski Z., Hobbs HH., Rosenbaum DM. DIP original experiment: DIP-268789X Nature 2016 IMEx Crystal structure of the human sterol transporter ABCG5/ABCG8. q9h222-1 ATP-binding cassette sub-family G member 5 Sterolin-1 ABCG5 protein protein human Homo sapiens Human MGDLSSLTPGGSMGLQVNRGSQSSLEGAPATAPEPHSLGILHASYSVSHRVRPWWDITSCRQQWTRQILKDVSLYVESGQIMCILGSSGSGKTTLLDAMSGRLGRAGTFLGEVYVNGRALRREQFQDCFSYVLQSDTLLSSLTVRETLHYTALLAIRRGNPGSFQKKVEAVMAELSLSHVADRLIGNYSLGGISTGERRRVSIAAQLLQDPKVMLFDEPTTGLDCMTANQIVVLLVELARRNRIVVLTIHQPRSELFQLFDKIAILSFGELIFCGTPAEMLDFFNDCGYPCPEHSNPFDFYMDLTSVDTQSKEREIETSKRVQMIESAYKKSAICHKTLKNIERMKHLKTLPMVPFKTKDSPGVFSKLGVLLRRVTRNLVRNKLAVITRLLQNLIMGLFLLFFVLRVRSNVLKGAIQDRVGLLYQFVGATPYTGMLNAVNLFPVLRAVSDQESQDGLYQKWQMMLAYALHVLPFSVVATMIFSSVCYWTLGLHPEVARFGYFSAALLAPHLIGEFLTLVLLGIVQNPNIVNSVVALLSIAGVLVGSGFLRNIQEMPIPFKIISYFTFQKYCSEILVVNEFYGLNFTCGSSNVSVTTNPMCAFTQGIQFIEKTCPGATSRFTMNFLILYSFIPALVILGIVVFKIRDHLISR DIP protein Q9H222-1 original sequence version: 144 q9h221-1 ATP-binding cassette sub-family G member 8 Sterolin-2 ABCG8 protein protein human Homo sapiens Human MAGKAAEERGLPKGATPQDTSGLQDRLFSSESDNSLYFTYSGQPNTLEVRDLNYQVDLASQVPWFEQLAQFKMPWTSPSCQNSCELGIQNLSFKVRSGQMLAIIGSSGCGRASLLDVITGRGHGGKIKSGQIWINGQPSSPQLVRKCVAHVRQHNQLLPNLTVRETLAFIAQMRLPRTFSQAQRDKRVEDVIAELRLRQCADTRVGNMYVRGLSGGERRRVSIGVQLLWNPGILILDEPTSGLDSFTAHNLVKTLSRLAKGNRLVLISLHQPRSDIFRLFDLVLLMTSGTPIYLGAAQHMVQYFTAIGYPCPRYSNPADFYVDLTSIDRRSREQELATREKAQSLAALFLEKVRDLDDFLWKAETKDLDEDTCVESSVTPLDTNCLPSPTKMPGAVQQFTTLIRRQISNDFRDLPTLLIHGAEACLMSMTIGFLYFGHGSIQLSFMDTAALLFMIGALIPFNVILDVISKCYSERAMLYYELEDGLYTTGPYFFAKILGELPEHCAYIIIYGMPTYWLANLRPGLQPFLLHFLLVWLVVFCCRIMALAAAALLPTFHMASFFSNALYNSFYLAGGFMINLSSLWTVPAWISKVSFLRWCFEGLMKIQFSRRTYKMPLGNLTIAVSGDKILSVMELDSYPLYAIYLIVIGLSGGFMVLYYVSLRFIKQKPSQDW DIP protein Q9H221-1 original sequence version: 146 abcg5-abcg8-1 2 7 unspecified role unspecified role unspecified role unspecified role picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 6 unspecified role unspecified role unspecified role unspecified role picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction abcg8-abcg5-1 3 7 unspecified role unspecified role bait bait CBP-tag CBP-tag cam tag calmodulin binding protein tag cam tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 6 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range picpa Pichia pastoris (Yeast) Yeast Pichia pastoris physical association physical association abcg5-abcg8-2 5 7 unspecified role unspecified role unspecified role unspecified role picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 6 unspecified role unspecified role unspecified role unspecified role picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction abcg5-abcg8-3 4 7 unspecified role unspecified role unspecified role unspecified role picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 6 unspecified role unspecified role unspecified role unspecified role picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction abcg5-abcg8-4 1 7 unspecified role unspecified role prey prey CBP-tag CBP-tag cam tag calmodulin binding protein tag cam tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 6 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range picpa Pichia pastoris (Yeast) Yeast Pichia pastoris physical association physical association