DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Architecture of the mitochondrial calcium uniporter. Architecture of the mitochondrial calcium uniporter. Nature 2016 imex curation Oxenoid K., Dong Y., Cao C., Cui T., Sancak Y., Markhard AL., Grabarek Z., Kong L., Liu Z., Ouyang B., Cong Y., Mootha VK., Chou JJ. Only protein-protein interactions ecolx Escherichia coli solution nmr solution state nmr solution state nmr solution nmr predetermined predetermined participant predetermined Oxenoid K., Dong Y., Cao C., Cui T., Sancak Y., Markhard AL., Grabarek Z., Kong L., Liu Z., Ouyang B., Cong Y., Mootha VK., Chou JJ. DIP original experiment: DIP-268772X Nature 2016 IMEx Architecture of the mitochondrial calcium uniporter. Architecture of the mitochondrial calcium uniporter. Architecture of the mitochondrial calcium uniporter. Nature 2016 imex curation Oxenoid K., Dong Y., Cao C., Cui T., Sancak Y., Markhard AL., Grabarek Z., Kong L., Liu Z., Ouyang B., Cong Y., Mootha VK., Chou JJ. Only protein-protein interactions ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Oxenoid K., Dong Y., Cao C., Cui T., Sancak Y., Markhard AL., Grabarek Z., Kong L., Liu Z., Ouyang B., Cong Y., Mootha VK., Chou JJ. DIP original experiment: DIP-268771X Nature 2016 IMEx Architecture of the mitochondrial calcium uniporter. Architecture of the mitochondrial calcium uniporter. Architecture of the mitochondrial calcium uniporter. Nature 2016 imex curation Oxenoid K., Dong Y., Cao C., Cui T., Sancak Y., Markhard AL., Grabarek Z., Kong L., Liu Z., Ouyang B., Cong Y., Mootha VK., Chou JJ. Only protein-protein interactions ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Oxenoid K., Dong Y., Cao C., Cui T., Sancak Y., Markhard AL., Grabarek Z., Kong L., Liu Z., Ouyang B., Cong Y., Mootha VK., Chou JJ. DIP original experiment: DIP-268773X Nature 2016 IMEx Architecture of the mitochondrial calcium uniporter. Architecture of the mitochondrial calcium uniporter. Architecture of the mitochondrial calcium uniporter. Nature 2016 imex curation Oxenoid K., Dong Y., Cao C., Cui T., Sancak Y., Markhard AL., Grabarek Z., Kong L., Liu Z., Ouyang B., Cong Y., Mootha VK., Chou JJ. Only protein-protein interactions ecolx Escherichia coli crosslink cross-linking study crosslink weight silver stain molecular weight estimation by silver staining weight silver stain Oxenoid K., Dong Y., Cao C., Cui T., Sancak Y., Markhard AL., Grabarek Z., Kong L., Liu Z., Ouyang B., Cong Y., Mootha VK., Chou JJ. DIP original experiment: DIP-268774X Nature 2016 IMEx Architecture of the mitochondrial calcium uniporter. Architecture of the mitochondrial calcium uniporter. Architecture of the mitochondrial calcium uniporter. Nature 2016 imex curation Oxenoid K., Dong Y., Cao C., Cui T., Sancak Y., Markhard AL., Grabarek Z., Kong L., Liu Z., Ouyang B., Cong Y., Mootha VK., Chou JJ. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink weight silver stain molecular weight estimation by silver staining weight silver stain Oxenoid K., Dong Y., Cao C., Cui T., Sancak Y., Markhard AL., Grabarek Z., Kong L., Liu Z., Ouyang B., Cong Y., Mootha VK., Chou JJ. DIP original experiment: DIP-268775X Nature 2016 IMEx Architecture of the mitochondrial calcium uniporter. Architecture of the mitochondrial calcium uniporter. Architecture of the mitochondrial calcium uniporter. Nature 2016 imex curation Oxenoid K., Dong Y., Cao C., Cui T., Sancak Y., Markhard AL., Grabarek Z., Kong L., Liu Z., Ouyang B., Cong Y., Mootha VK., Chou JJ. Only protein-protein interactions in vitro In vitro solution nmr solution state nmr solution state nmr solution nmr predetermined predetermined participant predetermined Oxenoid K., Dong Y., Cao C., Cui T., Sancak Y., Markhard AL., Grabarek Z., Kong L., Liu Z., Ouyang B., Cong Y., Mootha VK., Chou JJ. DIP original experiment: DIP-268776X Nature 2016 IMEx Architecture of the mitochondrial calcium uniporter. Architecture of the mitochondrial calcium uniporter. Architecture of the mitochondrial calcium uniporter. Nature 2016 imex curation Oxenoid K., Dong Y., Cao C., Cui T., Sancak Y., Markhard AL., Grabarek Z., Kong L., Liu Z., Ouyang B., Cong Y., Mootha VK., Chou JJ. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Oxenoid K., Dong Y., Cao C., Cui T., Sancak Y., Markhard AL., Grabarek Z., Kong L., Liu Z., Ouyang B., Cong Y., Mootha VK., Chou JJ. DIP original experiment: DIP-268770X Nature 2016 IMEx Architecture of the mitochondrial calcium uniporter. mcu_caeel Calcium uniporter protein, mitochondrial K02B2.3 mcu-1 protein protein caeel Caenorhabditis elegans MRNGRCLVTPFVTAQRLANLRNTLWNRQQIAFSTTTASSSTSPIQESSSPLSIRFEYGLPLLDVPLPSRNEPCQFTMRPLSDTIGSLCEFLRQEDRGIDYVAVYGTNGVKLATCTSIEHLLQFGSFRLRLNDKFFDVTVPKTGTMPYDSDKLRQLDDLRATVASLHAALCVDEYKLSREKKLLLQLENAETLLAPLHDAKRKIEQECEAHTDRVMWAGFAAMGVQTGLFARLTWWEYSWDIMEPVTYFATYSTVCATFGYYLYTQQSFEYPSARERVYTKQFYRRAQKQNFDIEKYNRLVTEVDELRNQLKRMRDPLFQHLPVSYLSNLEAEK DIP protein Q21121 original sequence version: 92 14398F25E6C4D4EE mcu_1-1 3 8 unspecified role unspecified role unspecified role unspecified role Region 167-316 Region 167-316 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction mcu_1-3 7 8 unspecified role unspecified role unspecified role unspecified role Region 167-316 Region 167-316 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction mcu_1-4 4 8 unspecified role unspecified role unspecified role unspecified role Region 167-316 Region 167-316 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction mcu_1-5 2 8 unspecified role unspecified role unspecified role unspecified role Region 167-316 Region 167-316 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction mcu_1-6 5 8 unspecified role unspecified role unspecified role unspecified role Region 288-316 Region 288-316 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction mcu_1-7 1 8 unspecified role unspecified role unspecified role unspecified role Region 167-316 Region 167-316 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction mcu_1-8 6 8 unspecified role unspecified role unspecified role unspecified role Region 167-316 Region 167-316 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction