DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural and molecular basis for Ebola virus neutralization by protective human antibodies. Structural and molecular basis for Ebola virus neutralization by protective human antibodies. Science (New York, N.Y.) 2016 imex curation Misasi J., Gilman MS., Kanekiyo M., Gui M., Cagigi A., Mulangu S., Corti D., Ledgerwood JE., Lanzavecchia A., Cunningham J., Muyembe-Tamfun JJ., Baxa U., Graham BS., Xiang Y., Sullivan NJ., McLellan JS. Only protein-protein interactions human Homo sapiens Human x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Misasi J., Gilman MS., Kanekiyo M., Gui M., Cagigi A., Mulangu S., Corti D., Ledgerwood JE., Lanzavecchia A., Cunningham J., Muyembe-Tamfun JJ., Baxa U., Graham BS., Xiang Y., Sullivan NJ., McLellan JS. DIP original experiment: DIP-269019X Science (New York, N.Y.) 2016 IMEx Structural and molecular basis for Ebola virus neutralization by protective human antibodies. Structural and molecular basis for Ebola virus neutralization by protective human antibodies. Structural and molecular basis for Ebola virus neutralization by protective human antibodies. Science (New York, N.Y.) 2016 imex curation Misasi J., Gilman MS., Kanekiyo M., Gui M., Cagigi A., Mulangu S., Corti D., Ledgerwood JE., Lanzavecchia A., Cunningham J., Muyembe-Tamfun JJ., Baxa U., Graham BS., Xiang Y., Sullivan NJ., McLellan JS. Only protein-protein interactions human Homo sapiens Human tem transmission electron microscopy tem predetermined predetermined participant predetermined Misasi J., Gilman MS., Kanekiyo M., Gui M., Cagigi A., Mulangu S., Corti D., Ledgerwood JE., Lanzavecchia A., Cunningham J., Muyembe-Tamfun JJ., Baxa U., Graham BS., Xiang Y., Sullivan NJ., McLellan JS. DIP original experiment: DIP-269021X Science (New York, N.Y.) 2016 IMEx Structural and molecular basis for Ebola virus neutralization by protective human antibodies. Structural and molecular basis for Ebola virus neutralization by protective human antibodies. Structural and molecular basis for Ebola virus neutralization by protective human antibodies. Science (New York, N.Y.) 2016 imex curation Misasi J., Gilman MS., Kanekiyo M., Gui M., Cagigi A., Mulangu S., Corti D., Ledgerwood JE., Lanzavecchia A., Cunningham J., Muyembe-Tamfun JJ., Baxa U., Graham BS., Xiang Y., Sullivan NJ., McLellan JS. Only protein-protein interactions human Homo sapiens Human 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Misasi J., Gilman MS., Kanekiyo M., Gui M., Cagigi A., Mulangu S., Corti D., Ledgerwood JE., Lanzavecchia A., Cunningham J., Muyembe-Tamfun JJ., Baxa U., Graham BS., Xiang Y., Sullivan NJ., McLellan JS. DIP original experiment: DIP-269020X Science (New York, N.Y.) 2016 IMEx Structural and molecular basis for Ebola virus neutralization by protective human antibodies. Structural and molecular basis for Ebola virus neutralization by protective human antibodies. Structural and molecular basis for Ebola virus neutralization by protective human antibodies. Science (New York, N.Y.) 2016 imex curation Misasi J., Gilman MS., Kanekiyo M., Gui M., Cagigi A., Mulangu S., Corti D., Ledgerwood JE., Lanzavecchia A., Cunningham J., Muyembe-Tamfun JJ., Baxa U., Graham BS., Xiang Y., Sullivan NJ., McLellan JS. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Misasi J., Gilman MS., Kanekiyo M., Gui M., Cagigi A., Mulangu S., Corti D., Ledgerwood JE., Lanzavecchia A., Cunningham J., Muyembe-Tamfun JJ., Baxa U., Graham BS., Xiang Y., Sullivan NJ., McLellan JS. DIP original experiment: DIP-269022X Science (New York, N.Y.) 2016 IMEx Structural and molecular basis for Ebola virus neutralization by protective human antibodies. vgp_ebozm Envelope glycoprotein GP GP1,2 protein protein ebozm Zaire ebolavirus (strain Mayinga-76) ZEBOV Zaire Ebola virus MGVTGILQLPRDRFKRTSFFLWVIILFQRTFSIPLGVIHNSTLQVSDVDKLVCRDKLSSTNQLRSVGLNLEGNGVATDVPSATKRWGFRSGVPPKVVNYEAGEWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHKVSGTGPCAGDFAFHKEGAFFLYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTIRYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYTSGKRSNTTGKLIWKVNPEIDTTIGEWAFWETKKNLTRKIRSEELSFTVVSNGAKNISGQSPARTSSDPGTNTTTEDHKIMASENSSAMVQVHSQGREAAVSHLTTLATISTSPQSLTTKPGPDNSTHNTPVYKLDISEATQVEQHHRRTDNDSTASDTPSATTAAGPPKAENTNTSKSTDFLDPATTTSPQNHSETAGNNNTHHQDTGEESASSGKLGLITNTIAGVAGLITGGRRTRREAIVNAQPKCNPNLHYWTTQDEGAAIGLAWIPYFGPAAEGIYIEGLMHNQDGLICGLRQLANETTQALQLFLRATTELRTFSILNRKAIDFLLQRWGGTCHILGPDCCIEPHDWTKNITDKIDQIIHDFVDKTLPDQGDNDNWWTGWRQWIPAGIGVTGVIIAVIALFCICKFVF DIP protein Q05320 original sequence version: 115 npc1_human NPC intracellular cholesterol transporter 1 NPC1 Niemann-Pick C1 protein protein protein human Homo sapiens Human MTARGLALGLLLLLLCPAQVFSQSCVWYGECGIAYGDKRYNCEYSGPPKPLPKDGYDLVQELCPGFFFGNVSLCCDVRQLQTLKDNLQLPLQFLSRCPSCFYNLLNLFCELTCSPRQSQFLNVTATEDYVDPVTNQTKTNVKELQYYVGQSFANAMYNACRDVEAPSSNDKALGLLCGKDADACNATNWIEYMFNKDNGQAPFTITPVFSDFPVHGMEPMNNATKGCDESVDEVTAPCSCQDCSIVCGPKPQPPPPPAPWTILGLDAMYVIMWITYMAFLLVFFGAFFAVWCYRKRYFVSEYTPIDSNIAFSVNASDKGEASCCDPVSAAFEGCLRRLFTRWGSFCVRNPGCVIFFSLVFITACSSGLVFVRVTTNPVDLWSAPSSQARLEKEYFDQHFGPFFRTEQLIIRAPLTDKHIYQPYPSGADVPFGPPLDIQILHQVLDLQIAIENITASYDNETVTLQDICLAPLSPYNTNCTILSVLNYFQNSHSVLDHKKGDDFFVYADYHTHFLYCVRAPASLNDTSLLHDPCLGTFGGPVFPWLVLGGYDDQNYNNATALVITFPVNNYYNDTEKLQRAQAWEKEFINFVKNYKNPNLTISFTAERSIEDELNRESDSDVFTVVISYAIMFLYISLALGHMKSCRRLLVDSKVSLGIAGILIVLSSVACSLGVFSYIGLPLTLIVIEVIPFLVLAVGVDNIFILVQAYQRDERLQGETLDQQLGRVLGEVAPSMFLSSFSETVAFFLGALSVMPAVHTFSLFAGLAVFIDFLLQITCFVSLLGLDIKRQEKNRLDIFCCVRGAEDGTSVQASESCLFRFFKNSYSPLLLKDWMRPIVIAIFVGVLSFSIAVLNKVDIGLDQSLSMPDDSYMVDYFKSISQYLHAGPPVYFVLEEGHDYTSSKGQNMVCGGMGCNNDSLVQQIFNAAQLDNYTRIGFAPSSWIDDYFDWVKPQSSCCRVDNITDQFCNASVVDPACVRCRPLTPEGKQRPQGGDFMRFLPMFLSDNPNPKCGKGGHAAYSSAVNILLGHGTRVGATYFMTYHTVLQTSADFIDALKKARLIASNVTETMGINGSAYRVFPYSVFYVFYEQYLTIIDDTIFNLGVSLGAIFLVTMVLLGCELWSAVIMCATIAMVLVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHITRAFTVSMKGSRVERAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFQIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKSCATEERYKGTERERLLNF DIP protein O15118 original sequence version: 157 DA1523E09822E5C7 npc1-gp-1 4 6 unspecified role unspecified role bait bait Region 371-621 Region 371-621 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 5 unspecified role unspecified role prey prey Region 33-308/502-599 Region 33-308/502-599 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human direct interaction direct interaction gp-4 1 5 unspecified role unspecified role unspecified role unspecified role Region 33-308/502-599 Region 33-308/502-599 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human direct interaction direct interaction gp-2 3 5 unspecified role unspecified role unspecified role unspecified role Region 33-308/502-599 Region 33-308/502-599 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human direct interaction direct interaction gp-3 2 5 unspecified role unspecified role unspecified role unspecified role Region 33-308/502-599 Region 33-308/502-599 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human direct interaction direct interaction