IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structural Characterization of the Extracellular Domain of CASPR2 and Insights into Its Association with the Novel Ligand Contactin1. Structural Characterization of the Extracellular Domain of CASPR2 and Insights into Its Association with the Novel Ligand Contactin1. The Journal of biological chemistry 2016 imex curation Rubio-Marrero EN., Vincelli G., Jeffries CM., Shaikh TR., Pakos IS., Ranaivoson FM., von Daake S., Demeler B., De Jaco A., Perkins G., Ellisman MH., Trewhella J., Comoletti D., Rubio-Marrero EN., Vincelli G., Jeffries CM., Shaikh TR., Pakos IS., Ranaivoson FM., von Daake S., Demeler B., De Jaco A., Perkins G., Ellisman MH., Trewhella J., Comoletti D. Cells used for ECD expression were confirmed by the authors to be "HEK293S GnTI−cells". the editor tells me this publication has already been curated by another group; but not by DIP when searching the DIP database directly (Imex central is down - nbg) and PSICQUIC page says no interactions on other sevices (may be errors here - but all "green" apart from DIP). So probably an (extinct) Lukash "reservation".. comoleda@rwjms.rutgers.edu 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Rubio-Marrero EN., Vincelli G., Jeffries CM., Shaikh TR., Pakos IS., Ranaivoson FM., von Daake S., Demeler B., De Jaco A., Perkins G., Ellisman MH., Trewhella J., Comoletti D., Rubio-Marrero EN., Vincelli G., Jeffries CM., Shaikh TR., Pakos IS., Ranaivoson FM., von Daake S., Demeler B., De Jaco A., Perkins G., Ellisman MH., Trewhella J., Comoletti D. the editor tells me this publication has already been curated by another group; but not by DIP when searching the DIP database directly (Imex central is down - nbg) and PSICQUIC page says no interactions on other sevices (may be errors here - but all "green" apart from DIP). So probably an (extinct) Lukash "reservation".. comoleda@rwjms.rutgers.edu Accepted 2017-NOV-17 AT 14:08 GMT AT 14:08 GMT by LIVIAP The Journal of biological chemistry 2016 imex curation Structural Characterization of the Extracellular Domain of CASPR2 and Insights into Its Association with the Novel Ligand Contactin1. Structural Characterization of the Extracellular Domain of CASPR2 and Insights into Its Association with the Novel Ligand Contactin1. The Journal of biological chemistry 2016 imex curation Rubio-Marrero EN., Vincelli G., Jeffries CM., Shaikh TR., Pakos IS., Ranaivoson FM., von Daake S., Demeler B., De Jaco A., Perkins G., Ellisman MH., Trewhella J., Comoletti D., Rubio-Marrero EN., Vincelli G., Jeffries CM., Shaikh TR., Pakos IS., Ranaivoson FM., von Daake S., Demeler B., De Jaco A., Perkins G., Ellisman MH., Trewhella J., Comoletti D. Cells used for ECD expression were confirmed by the authors to be "HEK293S GnTI−cells". the editor tells me this publication has already been curated by another group; but not by DIP when searching the DIP database directly (Imex central is down - nbg) and PSICQUIC page says no interactions on other sevices (may be errors here - but all "green" apart from DIP). So probably an (extinct) Lukash "reservation".. comoleda@rwjms.rutgers.edu 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Rubio-Marrero EN., Vincelli G., Jeffries CM., Shaikh TR., Pakos IS., Ranaivoson FM., von Daake S., Demeler B., De Jaco A., Perkins G., Ellisman MH., Trewhella J., Comoletti D., Rubio-Marrero EN., Vincelli G., Jeffries CM., Shaikh TR., Pakos IS., Ranaivoson FM., von Daake S., Demeler B., De Jaco A., Perkins G., Ellisman MH., Trewhella J., Comoletti D. the editor tells me this publication has already been curated by another group; but not by DIP when searching the DIP database directly (Imex central is down - nbg) and PSICQUIC page says no interactions on other sevices (may be errors here - but all "green" apart from DIP). So probably an (extinct) Lukash "reservation".. It is stated that "glycosylation deficient HEK293 GnTI− cells" were used for the expression of CASPR2 (CNTN1/TAG-1) ECD. comoleda@rwjms.rutgers.edu Accepted 2017-NOV-17 AT 14:08 GMT AT 14:08 GMT by LIVIAP The Journal of biological chemistry 2016 imex curation Structural Characterization of the Extracellular Domain of CASPR2 and Insights into Its Association with the Novel Ligand Contactin1. Structural Characterization of the Extracellular Domain of CASPR2 and Insights into Its Association with the Novel Ligand Contactin1. The Journal of biological chemistry 2016 imex curation Rubio-Marrero EN., Vincelli G., Jeffries CM., Shaikh TR., Pakos IS., Ranaivoson FM., von Daake S., Demeler B., De Jaco A., Perkins G., Ellisman MH., Trewhella J., Comoletti D., Rubio-Marrero EN., Vincelli G., Jeffries CM., Shaikh TR., Pakos IS., Ranaivoson FM., von Daake S., Demeler B., De Jaco A., Perkins G., Ellisman MH., Trewhella J., Comoletti D. Cells used for ECD expression were confirmed by the authors to be "HEK293S GnTI−cells". the editor tells me this publication has already been curated by another group; but not by DIP when searching the DIP database directly (Imex central is down - nbg) and PSICQUIC page says no interactions on other sevices (may be errors here - but all "green" apart from DIP). So probably an (extinct) Lukash "reservation".. comoleda@rwjms.rutgers.edu 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Rubio-Marrero EN., Vincelli G., Jeffries CM., Shaikh TR., Pakos IS., Ranaivoson FM., von Daake S., Demeler B., De Jaco A., Perkins G., Ellisman MH., Trewhella J., Comoletti D., Rubio-Marrero EN., Vincelli G., Jeffries CM., Shaikh TR., Pakos IS., Ranaivoson FM., von Daake S., Demeler B., De Jaco A., Perkins G., Ellisman MH., Trewhella J., Comoletti D. the editor tells me this publication has already been curated by another group; but not by DIP when searching the DIP database directly (Imex central is down - nbg) and PSICQUIC page says no interactions on other sevices (may be errors here - but all "green" apart from DIP). So probably an (extinct) Lukash "reservation".. Cell-based Binding Assay: Transfected 293 cells were incubated with purified proteins before fixation. comoleda@rwjms.rutgers.edu Accepted 2017-NOV-17 AT 14:08 GMT AT 14:08 GMT by LIVIAP The Journal of biological chemistry 2016 imex curation cntn1_human Contactin-1 CNTN1 Glycoprotein gp135 Neural cell surface protein F3 protein protein human Homo sapiens Human MKMWLLVSHLVIISITTCLAEFTWYRRYGHGVSEEDKGFGPIFEEQPINTIYPEESLEGKVSLNCRARASPFPVYKWRMNNGDVDLTSDRYSMVGGNLVINNPDKQKDAGIYYCLASNNYGMVRSTEATLSFGYLDPFPPEERPEVRVKEGKGMVLLCDPPYHFPDDLSYRWLLNEFPVFITMDKRRFVSQTNGNLYIANVEASDKGNYSCFVSSPSITKSVFSKFIPLIPIPERTTKPYPADIVVQFKDVYALMGQNVTLECFALGNPVPDIRWRKVLEPMPSTAEISTSGAVLKIFNIQLEDEGIYECEAENIRGKDKHQARIYVQAFPEWVEHINDTEVDIGSDLYWPCVATGKPIPTIRWLKNGYAYHKGELRLYDVTFENAGMYQCIAENTYGAIYANAELKILALAPTFEMNPMKKKILAAKGGRVIIECKPKAAPKPKFSWSKGTEWLVNSSRILIWEDGSLEINNITRNDGGIYTCFAENNRGKANSTGTLVITDPTRIILAPINADITVGENATMQCAASFDPALDLTFVWSFNGYVIDFNKENIHYQRNFMLDSNGELLIRNAQLKHAGRYTCTAQTIVDNSSASADLVVRGPPGPPGGLRIEDIRATSVALTWSRGSDNHSPISKYTIQTKTILSDDWKDAKTDPPIIEGNMEAARAVDLIPWMEYEFRVVATNTLGRGEPSIPSNRIKTDGAAPNVAPSDVGGGGGRNRELTITWAPLSREYHYGNNFGYIVAFKPFDGEEWKKVTVTNPDTGRYVHKDETMSPSTAFQVKVKAFNNKGDGPYSLVAVINSAQDAPSEAPTEVGVKVLSSSEISVHWEHVLEKIVESYQIRYWAAHDKEEAANRVQVTSQEYSARLENLLPDTQYFIEVGACNSAGCGPPSDMIEAFTKKAPPSQPPRIISSVRSGSRYIITWDHVVALSNESTVTGYKVLYRPDGQHDGKLYSTHKHSIEVPIPRDGEYVVEVRAHSDGGDGVVSQVKISGAPTLSPSLLGLLLPAFGILVYLEF DIP protein Q12860 original sequence version: 124 4B8FDC5BFD434ED5 cntp2_human Contactin-associated protein-like 2 CNTNAP2 CASPR2 KIAA0868 Cell recognition molecule Caspr2 protein protein human Homo sapiens Human MQAAPRAGCGAALLLWIVSSCLCRAWTAPSTSQKCDEPLVSGLPHVAFSSSSSISGSYSPGYAKINKRGGAGGWSPSDSDHYQWLQVDFGNRKQISAIATQGRYSSSDWVTQYRMLYSDTGRNWKPYHQDGNIWAFPGNINSDGVVRHELQHPIIARYVRIVPLDWNGEGRIGLRIEVYGCSYWADVINFDGHVVLPYRFRNKKMKTLKDVIALNFKTSESEGVILHGEGQQGDYITLELKKAKLVLSLNLGSNQLGPIYGHTSVMTGSLLDDHHWHSVVIERQGRSINLTLDRSMQHFRTNGEFDYLDLDYEITFGGIPFSGKPSSSSRKNFKGCMESINYNGVNITDLARRKKLEPSNVGNLSFSCVEPYTVPVFFNATSYLEVPGRLNQDLFSVSFQFRTWNPNGLLVFSHFADNLGNVEIDLTESKVGVHINITQTKMSQIDISSGSGLNDGQWHEVRFLAKENFAILTIDGDEASAVRTNSPLQVKTGEKYFFGGFLNQMNNSSHSVLQPSFQGCMQLIQVDDQLVNLYEVAQRKPGSFANVSIDMCAIIDRCVPNHCEHGGKCSQTWDSFKCTCDETGYSGATCHNSIYEPSCEAYKHLGQTSNYYWIDPDGSGPLGPLKVYCNMTEDKVWTIVSHDLQMQTPVVGYNPEKYSVTQLVYSASMDQISAITDSAEYCEQYVSYFCKMSRLLNTPDGSPYTWWVGKANEKHYYWGGSGPGIQKCACGIERNCTDPKYYCNCDADYKQWRKDAGFLSYKDHLPVSQVVVGDTDRQGSEAKLSVGPLRCQGDRNYWNAASFPNPSSYLHFSTFQGETSADISFYFKTLTPWGVFLENMGKEDFIKLELKSATEVSFSFDVGNGPVEIVVRSPTPLNDDQWHRVTAERNVKQASLQVDRLPQQIRKAPTEGHTRLELYSQLFVGGAGGQQGFLGCIRSLRMNGVTLDLEERAKVTSGFISGCSGHCTSYGTNCENGGKCLERYHGYSCDCSNTAYDGTFCNKDVGAFFEEGMWLRYNFQAPATNARDSSSRVDNAPDQQNSHPDLAQEEIRFSFSTTKAPCILLYISSFTTDFLAVLVKPTGSLQIRYNLGGTREPYNIDVDHRNMANGQPHSVNITRHEKTIFLKLDHYPSVSYHLPSSSDTLFNSPKSLFLGKVIETGKIDQEIHKYNTPGFTGCLSRVQFNQIAPLKAALRQTNASAHVHIQGELVESNCGASPLTLSPMSSATDPWHLDHLDSASADFPYNPGQGQAIRNGVNRNSAIIGGVIAVVIFTILCTLVFLIRYMFRHKGTYHTNEAKGAESAESADAAIMNNDPNFTETIDESKKEWLI CFB2CB55BEFB99C2 cntn1-cntnap2-1 2 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed conditioned medium conditioned medium Ig_regions sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 5 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 physical association physical association 5A, 6A, 8A Text above Figure 5 states:- "When the phases were reversed by immobilizing CASPR2-Fc to the sensor tip and using purified CNTN1–993 (Fig. 5B), similar results were obtained." However the image and image legend for Figure 5B shows a SEC (molecular sieving) experiment. cntn1-1 1 4 unspecified role unspecified role neutral component neutral component purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 direct interaction direct interaction 5B CNTN1 is likely a dimer in solution. CNTN1 is likely a dimer in solution. cntn1-cntnap2-2 3 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 4 unspecified role unspecified role neutral component neutral component purified purified Ig5_Fn1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 colocalization colocalization 5C, 6E Fig 6E:-I do NOT see any co-loc. on the image, but the authors seem to think there is....so have entered it. cntn1-cntnap2-4 2 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed conditioned medium conditioned medium c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range D1-D6 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 4 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 physical association physical association 6B cntn1-cntnap2-6 3 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 5 unspecified role unspecified role neutral component neutral component purified purified D1-D6 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 colocalization colocalization 6E cntn1-cntnap2-5 3 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range mouse-brain_hippocampal_neuron Mus musculus, brain, hippocampal neurons brain-hippocampus_neuron Hippocampal neuron 5 unspecified role unspecified role neutral component neutral component purified purified ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient Human 293s_gnti HEK293S GnTI -adenovirus 5 transformed, glycosylation deficient ATCC® CRL-3022 colocalization colocalization 7C