IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Functional Analysis of the Hsp93/ClpC Chaperone at the Chloroplast Envelope. Functional Analysis of the Hsp93/ClpC Chaperone at the Chloroplast Envelope. Plant physiology 2016 imex curation Flores-Pérez Ú., Bédard J., Tanabe N., Lymperopoulos P., Clarke AK., Jarvis P. Request via Pablo: see pmids: 26265777 and 28684427 The following protein-protein interaction is shown: Hsp93-V(ClpC1) (At5g50920) vs. Tic110 (At1g06950) This was shown by in vitro pull-down (Fig. 1B) and BiFC (Fig. 1C). paul.jarvis@plants.ox.ac.uk 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation arath-protoplast Arabidopsis thaliana protoplast Protoplast defined as a membrane-bound cell from which the outer wall has been partially or completely removed. bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Flores-Pérez Ú., Bédard J., Tanabe N., Lymperopoulos P., Clarke AK., Jarvis P. Request via Pablo: see pmids: 26265777 and 28684427 The following protein-protein interaction is shown: Hsp93-V(ClpC1) (At5g50920) vs. Tic110 (At1g06950) This was shown by in vitro pull-down (Fig. 1B) and BiFC (Fig. 1C). paul.jarvis@plants.ox.ac.uk Accepted 2018-JUN-20 AT 12:34 BST AT 12:34 BST by PPORRAS Plant physiology 2016 Only protein-protein interactions imex curation imex curation altered Functional Analysis of the Hsp93/ClpC Chaperone at the Chloroplast Envelope. Functional Analysis of the Hsp93/ClpC Chaperone at the Chloroplast Envelope. Plant physiology 2016 imex curation Flores-Pérez Ú., Bédard J., Tanabe N., Lymperopoulos P., Clarke AK., Jarvis P. Request via Pablo: see pmids: 26265777 and 28684427 The following protein-protein interaction is shown: Hsp93-V(ClpC1) (At5g50920) vs. Tic110 (At1g06950) This was shown by in vitro pull-down (Fig. 1B) and BiFC (Fig. 1C). paul.jarvis@plants.ox.ac.uk 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation arath-seedling Arabidopsis thaliana seedling crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Flores-Pérez Ú., Bédard J., Tanabe N., Lymperopoulos P., Clarke AK., Jarvis P. Fig 3C: I had thought the I772E mutation was "mutation of NO effect" until I read the text. Request via Pablo: see pmids: 26265777 and 28684427 The following protein-protein interaction is shown: Hsp93-V(ClpC1) (At5g50920) vs. Tic110 (At1g06950) This was shown by in vitro pull-down (Fig. 1B) and BiFC (Fig. 1C). Isolated chloroplasts treated with dithiobis succinimidylpropionate (DSP) cross-linker and then immunoprecipitated with an anti-flag antibody. paul.jarvis@plants.ox.ac.uk Accepted 2018-JUN-20 AT 12:34 BST AT 12:34 BST by PPORRAS Plant physiology 2016 Only protein-protein interactions imex curation imex curation altered Functional Analysis of the Hsp93/ClpC Chaperone at the Chloroplast Envelope. Functional Analysis of the Hsp93/ClpC Chaperone at the Chloroplast Envelope. Plant physiology 2016 imex curation Flores-Pérez Ú., Bédard J., Tanabe N., Lymperopoulos P., Clarke AK., Jarvis P. Request via Pablo: see pmids: 26265777 and 28684427 The following protein-protein interaction is shown: Hsp93-V(ClpC1) (At5g50920) vs. Tic110 (At1g06950) This was shown by in vitro pull-down (Fig. 1B) and BiFC (Fig. 1C). paul.jarvis@plants.ox.ac.uk 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Flores-Pérez Ú., Bédard J., Tanabe N., Lymperopoulos P., Clarke AK., Jarvis P. Request via Pablo: see pmids: 26265777 and 28684427 The following protein-protein interaction is shown: Hsp93-V(ClpC1) (At5g50920) vs. Tic110 (At1g06950) This was shown by in vitro pull-down (Fig. 1B) and BiFC (Fig. 1C). His-tagged recombinant proteins were incubated with a stromal protein fraction from isolated wild-type chloroplasts. paul.jarvis@plants.ox.ac.uk Accepted 2018-JUN-20 AT 12:34 BST AT 12:34 BST by PPORRAS Plant physiology 2016 Only protein-protein interactions imex curation imex curation altered clpc1_arath Chaperone protein ClpC1, chloroplastic ATP-dependent Clp protease ATP-binding subunit ClpC homolog 1 Casein lytic proteinase C1 Protein DE-REGULATED CAO ACCUMULATION 1 Protein IRON-RESCUED MUTANT 1 K3K7.7 HSP93-V IRM1 At5g50920 CLPC1 DCA1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAMATRVLAQSTPPSLACYQRNVPSRGSGRSRRSVKMMCSQLQVSGLRMQGFMGLRGNNALDTLGKSRQDFHSKVRQAMNVPKGKASRFTVKAMFERFTEKAIKVIMLAQEEARRLGHNFVGTEQILLGLIGEGTGIAAKVLKSMGINLKDARVEVEKIIGRGSGFVAVEIPFTPRAKRVLELSLEEARQLGHNYIGSEHLLLGLLREGEGVAARVLENLGADPSNIRTQVIRMVGENNEVTANVGGGSSSNKMPTLEEYGTNLTKLAEEGKLDPVVGRQPQIERVVQILGRRTKNNPCLIGEPGVGKTAIAEGLAQRIASGDVPETIEGKKVITLDMGLLVAGTKYRGEFEERLKKLMEEIRQSDEIILFIDEVHTLIGAGAAEGAIDAANILKPALARGELQCIGATTLDEYRKHIEKDPALERRFQPVKVPEPTVDETIQILKGLRERYEIHHKLRYTDESLVAAAQLSYQYISDRFLPDKAIDLIDEAGSRVRLRHAQVPEEARELEKELRQITKEKNEAVRGQDFEKAGTLRDREIELRAEVSAIQAKGKEMSKAESETGEEGPMVTESDIQHIVSSWTGIPVEKVSTDESDRLLKMEETLHKRIIGQDEAVKAISRAIRRARVGLKNPNRPIASFIFSGPTGVGKSELAKALAAYYFGSEEAMIRLDMSEFMERHTVSKLIGSPPGYVGYTEGGQLTEAVRRRPYTVVLFDEIEKAHPDVFNMMLQILEDGRLTDSKGRTVDFKNTLLIMTSNVGSSVIEKGGRRIGFDLDYDEKDSSYNRIKSLVTEELKQYFRPEFLNRLDEMIVFRQLTKLEVKEIADILLKEVFERLKKKEIELQVTERFKERVVDEGYNPSYGARPLRRAIMRLLEDSMAEKMLAREIKEGDSVIVDVDAEGNVTVLNGGSGTPTTSLEEQEDSLPVA 438DEA514125F0BF clpr4_arath ATP-dependent Clp protease proteolytic subunit-related protein 4, chloroplastic CLPR4 HON5 Protein HAPPY ON NORFLURAZON 5 FCAALL.413 dl4550c At4g17040 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEVAAATATSFTTLRARTSAIIPSSTRNLRSKPRFSSSSSLRASLSNGFLSPYTGGSISSDLCGAKLRAESLNPLNFSSSKPKRGVVTMVIPFSKGSAHEQPPPDLASYLFKNRIVYLGMSLVPSVTELILAEFLYLQYEDEEKPIYLYINSTGTTKNGEKLGYDTEAFAIYDVMGYVKPPIFTLCVGNAWGEAALLLTAGAKGNRSALPSSTIMIKQPIARFQGQATDVEIARKEIKHIKTEMVKLYSKHIGKSPEQIEADMKRPKYFSPTEAVEYGIIDKVVYNERGSQDRGVVSDLKKAQLI 7902D9ECF220B92F clpp6_arath ATP-dependent Clp protease proteolytic subunit 6, chloroplastic NCLPP1 NCLPP6 Endopeptidase ClpP6 nClpP1 F25C20.10 CLPP6 At1g11750 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAGLAISPPLGLSFSSRTRNPKPTSFLSHNQRNPIRRIVSALQSPYGDSLKAGLSSNVSGSPIKIDNKAPRFGVIEAKKGNPPVMPSVMTPGGPLDLSSVLFRNRIIFIGQPINAQVAQRVISQLVTLASIDDKSDILMYLNCPGGSTYSVLAIYDCMSWIKPKVGTVAFGVAASQGALLLAGGEKGMRYAMPNTRVMIHQPQTGCGGHVEDVRRQVNEAIEARQKIDRMYAAFTGQPLEKVQQYTERDRFLSASEALEFGLIDGLLETEY 5565ADB698D0C776 tic40_arath Protein TIC 40, chloroplastic TIC40 PDE120 At5g16620 Protein PIGMENT DEFECTIVE EMBRYO 120 Translocon at the inner envelope membrane of chloroplasts 40 MTG13.6 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MENLTLVSCSASSPKLLIGCNFTSSLKNPTGFSRRTPNIVLRCSKISASAQSQSPSSRPENTGEIVVVKQRSKAFASIFSSSRDQQTTSVASPSVPVPPPSSSTIGSPLFWIGVGVGLSALFSYVTSNLKKYAMQTAMKTMMNQMNTQNSQFNNSGFPSGSPFPFPFPPQTSPASSPFQSQSQSSGATVDVTATKVETPPSTKPKPTPAKDIEVDKPSVVLEASKEKKEEKNYAFEDISPEETTKESPFSNYAEVSETNSPKETRLFEDVLQNGAGPANGATASEVFQSLGGGKGGPGLSVEALEKMMEDPTVQKMVYPYLPEEMRNPETFKWMLKNPQYRQQLQDMLNNMSGSGEWDKRMTDTLKNFDLNSPEVKQQFNQIGLTPEEVISKIMENPDVAMAFQNPRVQAALMECSENPMNIMKYQNDKEVMDVFNKISQLFPGMTG 52D3E7222AB461CE ti110_arath Protein TIC110, chloroplastic TIC110 Translocon at the inner envelope membrane of chloroplasts 110 F4H5.1 F10K1.33 At1g06950 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MNPSLVTAINAPISPSPRSPLLSHFLPTLPHRFSKSECLSRRRYRVSFPRSSAASSDQLSVSTQAKNPGIHGNKKELTGLQPIVEKMTPPVRLATSAVVLAASLATGYGLGLRLAGSRNIAFGGAAVAGAAGGAVVYALNSAVPEVAAISLHNYVAEFEDPASVTKDDVEKIADRYGVNKGDEAFQAEICDIYCRYVTSVLPTEGQSLKGDEVAKIVKFKNALGIDEPDAAAMHMEIGRRIFRQRLETGEREGDAEQRRAFMRLVYVSALVFGDASSFLLPWKRVLKVTDAQVEIAIRENAKQLYAERLKLVGRDINVENLVDLRKSQLSFKLSDELAEDLFREHTRKVVVENISSALSILKSRTRAAKSLASVVEELEKVLEFNNLLVSLKSHSEADQFARGVGPISLIGDESDFERRMDDLKLLYRAYVTDALSGGRLEENKLVAMSQLRNILGLGKREAEAISVDVTSKSYRKRLANAVSSGDLEAQDSKAKYLQKLCEELHFDAQKAGAIHEEIYRQKLQQCVTDGELSDDNVAALLRLRVMLCIPQQTVDTAHAEICGTIFEKVVRDAISSGVDGYDAETRKSVRKAAHGLRLSRETAMSIASKAVRRVFTNYIRRARAAENRTDSAKELKKMIAFNTLVVTEMVADIKGESSDKAPEEDPVQEKEEDDEDEEWGSLESLRKTRPDKELAEKMGKPGQTEITLKDDLPDRDRIDLYKTYLLYCVTGEVTRIPFGAQITTKRDDSEYLLLNQLGGILGLSSKEIVNIHVGLAEQAFRQQAEVILADGQLTKARVEQLDELQKQVGLPQPQAEKVIKNITTTKMANAIETAVNQGRLNIKQIRELKEANVSLDSMIAVSLREKLFKKTVSDIFSSGTGEFDETEVYQTIPSDLSIDVEKAKRVVHDLAQSRLSNSLVQAVALLRQRNSKGVVLSLNDLLACDKAVPAEPMSWEVSEELSDLYAIYSKSDPKPAPEKVLRLQYLLGIDDSTATALREMEDGALSSAAEEGNFVF 814CCDE07EB5D0EA tic110-clpc1-1 3 4 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level arath-seedling Arabidopsis thaliana seedling 8 unspecified role unspecified role bait bait purified purified c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 1B tic110-clpc1-2 1 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c_terminus nyfp nYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c_terminus cyfp cYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 1C The truncated Hsp93 protein (Hsp93DeltaC) produced a stronger YFP signal than the full-length Hsp93 construct. clpc1-tic110-1 1 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c_terminus cyfp cYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c_terminus nyfp nYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 1C-2 weak interaction 600707-1 2 7 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level 6 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level 4 unspecified role unspecified role bait bait subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 3C clpc1-clpp6-1 2 6 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level 4 unspecified role unspecified role bait bait subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q9FI56:p.Ile772Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I E Wed May 02 15:19:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile772glu' Tue Jun 30 20:21:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile772Glu' physical association physical association 3Ci clpc1-clpp6-2 2 7 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level 4 unspecified role unspecified role bait bait subcellular prep subcellular preparation subcellular prep over-expressed over expressed level over-expressed Q9FI56:p.Ile772Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain I E Wed May 02 15:34:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile772glu' Tue Jun 30 19:11:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile772Glu' c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 3Cii