DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. Nature cell biology 2015 imex curation Johnston AB., Collins A., Goode BL. Only protein-protein interactions in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Johnston AB., Collins A., Goode BL. DIP original experiment: DIP-268004X Nature cell biology 2015 IMEx High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. Nature cell biology 2015 imex curation Johnston AB., Collins A., Goode BL. Only protein-protein interactions in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Johnston AB., Collins A., Goode BL. DIP original experiment: DIP-268010X Nature cell biology 2015 IMEx High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. Nature cell biology 2015 imex curation Johnston AB., Collins A., Goode BL. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Johnston AB., Collins A., Goode BL. DIP original experiment: DIP-268008X Nature cell biology 2015 IMEx High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. Nature cell biology 2015 imex curation Johnston AB., Collins A., Goode BL. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Johnston AB., Collins A., Goode BL. DIP original experiment: DIP-268007X Nature cell biology 2015 IMEx High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. Nature cell biology 2015 imex curation Johnston AB., Collins A., Goode BL. Only protein-protein interactions in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Johnston AB., Collins A., Goode BL. DIP original experiment: DIP-268003X Nature cell biology 2015 IMEx High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. Nature cell biology 2015 imex curation Johnston AB., Collins A., Goode BL. Only protein-protein interactions in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Johnston AB., Collins A., Goode BL. DIP original experiment: DIP-268001X Nature cell biology 2015 IMEx High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. Nature cell biology 2015 imex curation Johnston AB., Collins A., Goode BL. Only protein-protein interactions in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Johnston AB., Collins A., Goode BL. DIP original experiment: DIP-268005X Nature cell biology 2015 IMEx High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. Nature cell biology 2015 imex curation Johnston AB., Collins A., Goode BL. Only protein-protein interactions in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Johnston AB., Collins A., Goode BL. DIP original experiment: DIP-268006X Nature cell biology 2015 IMEx High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. Nature cell biology 2015 imex curation Johnston AB., Collins A., Goode BL. Only protein-protein interactions in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Johnston AB., Collins A., Goode BL. DIP original experiment: DIP-268002X Nature cell biology 2015 IMEx High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. Nature cell biology 2015 imex curation Johnston AB., Collins A., Goode BL. Only protein-protein interactions in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Johnston AB., Collins A., Goode BL. DIP original experiment: DIP-268000X Nature cell biology 2015 IMEx High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. Nature cell biology 2015 imex curation Johnston AB., Collins A., Goode BL. Only protein-protein interactions in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Johnston AB., Collins A., Goode BL. DIP original experiment: DIP-268011X Nature cell biology 2015 IMEx High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. Nature cell biology 2015 imex curation Johnston AB., Collins A., Goode BL. Only protein-protein interactions ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Johnston AB., Collins A., Goode BL. DIP original experiment: DIP-268009X Nature cell biology 2015 IMEx High-speed depolymerization at actin filament ends jointly catalysed by Twinfilin and Srv2/CAP. cap_yeast Adenylyl cyclase-associated protein SRV2 CAP1 YNL138W N1210 N1838 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPDSKYTMQGYNLVKLLKRLEEATARLEDVTIYQEGYIQNKLEASKNNKPSDSGADANTTNEPSAENAPEVEQDPKCITAFQSYIGENIDPLVELSGKIDTVVLDALQLLKGGFQSQLTFLRAAVRSRKPDYSSQTFADSLRPINENIIKLGQLKESNRQSKYFAYLSALSEGAPLFSWVAVDTPVSMVTDFKDAAQFWTNRILKEYRESDPNAVEWVKKFLASFDNLKAYIKEYHTTGVSWKKDGMDFADAMAQSTKNTGATSSPSPASATAAPAPPPPPPAPPASVFEISNDTPATSSDANKGGIGAVFAELNQGENITKGLKKVDKSQQTHKNPELRQSSTVSSTGSKSGPPPRPKKPSTLKTKRPPRKELVGNKWFIENYENETESLVIDANKDESIFIGKCSQVLVQIKGKVNAISLSETESCSVVLDSSISGMDVIKSNKFGIQVNHSLPQISIDKSDGGNIYLSKESLNTEIYTSCSTAINVNLPIGEDDDYVEFPIPEQMKHSFADGKFKSAVFEHAG 0EB4D41205E2D464 0EB4D41205E2D464 cofi_yeast Cofilin COF1 YLL050C L0596 Actin-depolymerizing factor 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSRSGVAVADESLTAFNDLKLGKKYKFILFGLNDAKTEIVVKETSTDPSYDAFLEKLPENDCLYAIYDFEYEINGNEGKRSKIVFFTWSPDTAPVRSKMVYASSKDALRRALNGVSTDVQGTDFSEVSYDSVLERVSRGAGSH 7A03747B0F21F22D 7A03747B0F21F22D acts_rabit Actin, alpha skeletal muscle ACTA1 ACTA Alpha-actin-1 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF DIP protein P68135 original sequence version: 41 DIP protein P68135 original sequence version: 27 DIP protein P68135 original sequence version: 28 DIP protein P68135 original sequence version: 96 DIP protein P68135 original sequence version: 29 DIP protein P68135 original sequence version: 89 DIP protein P68135 original sequence version: 39 DIP protein P68135 original sequence version: 42 DIP protein P68135 original sequence version: 59 DIP protein P68135 original sequence version: 55 DIP protein P68135 original sequence version: 60 DIP protein P68135 original sequence version: 65 DIP protein P68135 original sequence version: 67 DIP protein P68135 original sequence version: 63 DIP protein P68135 original sequence version: 68 DIP protein P68135 original sequence version: 79 DIP protein P68135 original sequence version: 88 DIP protein P68135 original sequence version: 84 DIP protein P68135 original sequence version: 101 DIP protein P68135 original sequence version: 94 DIP protein P68135 original sequence version: 95 DIP protein P68135 original sequence version: 97 DIP protein P68135 original sequence version: 100 DIP protein P68135 original sequence version: 102 DIP protein P68135 original sequence version: 83 DF2A3A046346A179 twf1_yeast Twinfilin-1 TWF1 YGR080W Twinfilin-A protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTQSGIVAEQALLHSLNENLSADGIVIIIAKISPDSTSVHQTQVARSFEELVQLASQEREPLYIFYKPEGLDKYFFVSFIPDGSPVRSRMLYASTKNTLARQVGSNSLSTEQPLITDAQDLVDLKNFDSARPAGQNKPLTHDEEMQIEINKQQALLRKNTSVKLVSQDSASPLSLTFRVNSEKPINEILDSEGKNLIIFQIDPSNETIQIVQSDTCPSVDELYIDLPGPSYTIFRQGDSSFFIYSCPSGSKVKDRMIYASNKNGFINYLKNDQKIAFSKVVEIGDFVELDKSLLMATNKEDSLDHGSNPDLPNKSNLKFNKPKGPLRKRRT D9213BBB2D953DBD D9213BBB2D953DBD acta1-21 10 15 unspecified role unspecified role unspecified role unspecified role rabit Oryctolagus cuniculus (Rabbit) Rabbit direct interaction direct interaction acta1-twf1-3 3 16 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role OG-label OG-label oregon green label oregon green label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit Oryctolagus cuniculus (Rabbit) Rabbit direct interaction direct interaction acta1-srv2-1 11 16 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 13 unspecified role unspecified role unspecified role unspecified role Region 1-259 Region 1-259 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role rabit Oryctolagus cuniculus (Rabbit) Rabbit physical association physical association acta1-cof1-1 5 14 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role rabit Oryctolagus cuniculus (Rabbit) Rabbit direct interaction direct interaction acta1-twf1-4 6 16 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role rabit Oryctolagus cuniculus (Rabbit) Rabbit direct interaction direct interaction acta1-cof1-2 8 14 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role OG-label OG-label oregon green label oregon green label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit Oryctolagus cuniculus (Rabbit) Rabbit direct interaction direct interaction acta1-28 1 15 unspecified role unspecified role unspecified role unspecified role OG-label OG-label oregon green label oregon green label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit Oryctolagus cuniculus (Rabbit) Rabbit direct interaction direct interaction acta1-twf1-5 4 16 unspecified role unspecified role unspecified role unspecified role Region 305-332 Region 305-332 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role rabit Oryctolagus cuniculus (Rabbit) Rabbit direct interaction direct interaction srv2-1 12 13 unspecified role unspecified role unspecified role unspecified role Region 1-259 Region 1-259 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction acta1-srv2-2 9 13 unspecified role unspecified role unspecified role unspecified role Region 1-259 Region 1-259 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role rabit Oryctolagus cuniculus (Rabbit) Rabbit direct interaction direct interaction acta1-twf1-6 7 16 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role OG-label OG-label oregon green label oregon green label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit Oryctolagus cuniculus (Rabbit) Rabbit direct interaction direct interaction srv2-twf1-1 2 16 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 13 unspecified role unspecified role unspecified role unspecified role Region 1-259 Region 1-259 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction