DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Nature chemical biology 2015 imex curation Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease Only protein-protein interactions in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268156X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Nature chemical biology 2015 imex curation Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268157X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Nature chemical biology 2015 imex curation Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease Only protein-protein interactions in vitro In vitro comigration in sds comigration in sds page comigration in sds western blot western blot Immuno blot Immuno blot Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268153X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Nature chemical biology 2015 imex curation Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease Only protein-protein interactions in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268151X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Nature chemical biology 2015 imex curation Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease Only protein-protein interactions in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268159X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Nature chemical biology 2015 imex curation Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease Only protein-protein interactions in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268154X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Nature chemical biology 2015 imex curation Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease Only protein-protein interactions in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268149X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Nature chemical biology 2015 imex curation Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease Only protein-protein interactions in vitro In vitro atomic force microsc atomic force microscopy AFM AFM atomic force microsc predetermined predetermined participant predetermined Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268148X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Nature chemical biology 2015 imex curation Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati weight by comassie molecular weight estimation by coomasie staining weight by comassie Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268152X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Nature chemical biology 2015 imex curation Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268155X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Nature chemical biology 2015 imex curation Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati weight silver stain molecular weight estimation by silver staining weight silver stain Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268158X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Nature chemical biology 2015 imex curation Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M., Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease Only protein-protein interactions in vitro In vitro atomic force microsc atomic force microscopy AFM AFM atomic force microsc predetermined predetermined participant predetermined Poepsel S., Sprengel A., Sacca B., Kaschani F., Kaiser M., Gatsogiannis C., Raunser S., Clausen T., Ehrmann M. Alzheimers - Interactions investigated in the context of Alzheimers disease DIP original experiment: DIP-268150X Nature chemical biology 2015 IMEx Determinants of amyloid fibril degradation by the PDZ protease HTRA1. p05067-pro_0000000092 Amyloid-beta protein 42 Alzheimer disease amyloid protein Cerebral vascular amyloid peptide APP A4 AD1 ABPP PreA4 Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 672 713 3AC85563D7858C37 htra1_human Serine protease HTRA1 HTRA1 HTRA PRSS11 High-temperature requirement A serine peptidase 1 L56 Serine protease 11 protein protein human Homo sapiens Human MQIPRAALLPLLLLLLAAPASAQLSRAGRSAPLAAGCPDRCEPARCPPQPEHCEGGRARDACGCCEVCGAPEGAACGLQEGPCGEGLQCVVPFGVPASATVRRRAQAGLCVCASSEPVCGSDANTYANLCQLRAASRRSERLHRPPVIVLQRGACGQGQEDPNSLRHKYNFIADVVEKIAPAVVHIELFRKLPFSKREVPVASGSGFIVSEDGLIVTNAHVVTNKHRVKVELKNGATYEAKIKDVDEKADIALIKIDHQGKLPVLLLGRSSELRPGEFVVAIGSPFSLQNTVTTGIVSTTQRGGKELGLRNSDMDYIQTDAIINYGNSGGPLVNLDGEVIGINTLKVTAGISFAIPSDKIKKFLTESHDRQAKGKAITKKKYIGIRMMSLTSSKAKELKDRHRDFPDVISGAYIIEVIPDTPAEAGGLKENDVIISINGQSVVSANDVSDVIKRESTLNMVVRRGNEDIMITVIPEEIDP DIP protein Q92743 original sequence version: 106 DIP protein Q92743 original sequence version: 149 DIP protein Q92743 original sequence version: 119 CA20A99480FB2330 p10636-8 Microtubule-associated protein tau Tau-4 MAPT MAPTL MTBT1 TAU Neurofibrillary tangle protein Paired helical filament-tau 2N4R protein protein human Homo sapiens Human MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL DIP protein P10636-8 original sequence version: 206 DIP protein P10636-8 original sequence version: 203 DIP protein P10636-8 original sequence version: 166 DIP protein P10636-8 original sequence version: 218 835A8706D847A8CC mapt-1 8 15 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction htra1-mapt-1 6 14 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli 15 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli protein cleavage protein cleavage htra1-mapt-2 1 14 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role DyLight488-label DyLight488-label fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction htra1-mapt-3 9 14 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role Region 258-360 Region 258-360 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction abeta(142)-18 2 13 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 13 unspecified role unspecified role unspecified role unspecified role in vitro In vitro direct interaction direct interaction mapt-2 7 15 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction htra1-mapt-4 10 14 unspecified role unspecified role unspecified role unspecified role Region 380-480 Region 380-480 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role Region 385-393 Region 385-393 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Region 308-319 Region 308-319 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction mapt-3 5 15 unspecified role unspecified role unspecified role unspecified role DyLight488-label DyLight488-label fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role DyLight488-label DyLight488-label fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction abeta(142)-htra1-1 11 14 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 13 unspecified role unspecified role unspecified role unspecified role in vitro In vitro direct interaction direct interaction htra1-mapt-5 12 14 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction mapt-4 3 15 unspecified role unspecified role unspecified role unspecified role Region 258-360 Region 258-360 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role Region 258-360 Region 258-360 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction htra1-mapt-6 4 14 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction