DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for gene regulation by a B12-dependent photoreceptor. Structural basis for gene regulation by a B12-dependent photoreceptor. Nature 2015 imex curation Jost M., Fernández-Zapata J., Polanco MC., Ortiz-Guerrero JM., Chen PY., Kang G., Padmanabhan S., Elías-Arnanz M., Drennan CL. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Jost M., Fernández-Zapata J., Polanco MC., Ortiz-Guerrero JM., Chen PY., Kang G., Padmanabhan S., Elías-Arnanz M., Drennan CL. DIP original experiment: DIP-267071X Nature 2015 IMEx Structural basis for gene regulation by a B12-dependent photoreceptor. Structural basis for gene regulation by a B12-dependent photoreceptor. Structural basis for gene regulation by a B12-dependent photoreceptor. Nature 2015 imex curation Jost M., Fernández-Zapata J., Polanco MC., Ortiz-Guerrero JM., Chen PY., Kang G., Padmanabhan S., Elías-Arnanz M., Drennan CL. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Jost M., Fernández-Zapata J., Polanco MC., Ortiz-Guerrero JM., Chen PY., Kang G., Padmanabhan S., Elías-Arnanz M., Drennan CL. DIP original experiment: DIP-267072X Nature 2015 IMEx Structural basis for gene regulation by a B12-dependent photoreceptor. q746j7_thet2 TT_P0056 protein protein thet2 Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) MTSSGVYTIAEVEAMTGLSAEVLRQWERRYGFPKPRRTPGGHRLYSAEDVEALKTIKRWLEEGATPKAAIRRYLAQEVRPEDLGTGLLEALLRGDLAGAEALFRRGLRFWGPEGVLEHLLLPVLREVGEAWHRGEIGVAEEHLASTFLRARLQELLDLAGFPPGPPVLVTTPPGERHEIGAMLAAYHLRRKGVPALYLGPDTPLPDLRALARRLGAGAVVLSAVLSEPLRALPDGALKDLAPRVFLGGQGAGPEEARRLGAEYMEDLKGLAEALWLPRGPEKEAI DIP protein Q746J7 original sequence version: 88 q746j7_thet2-1 1 3 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction q746j7_thet2-2 2 3 unspecified role unspecified role unspecified role unspecified role Q746J7:p.His142Ala H142A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain H A This feature has been corrected as a result of our quality control procedures. The original label was 'H142A'. Wed May 02 15:20:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his142ala' Tue Jun 30 18:30:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His142Ala' Q746J7:p.Asp201Arg D201R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain D R This feature has been corrected as a result of our quality control procedures. The original label was 'D201R'. Wed May 02 17:01:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp201arg' Tue Jun 30 17:30:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp201Arg' Q746J7:p.Trp131Ala W131A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W131A'. Wed May 02 18:42:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp131ala' Tue Jun 30 19:36:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp131Ala' Q746J7:p.Glu141Ala E141A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E141A'. Wed May 02 19:37:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu141ala' Tue Jun 30 17:59:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu141Ala' Q746J7:p.Gly160Gln G160Q mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain G Q This feature has been corrected as a result of our quality control procedures. The original label was 'G160Q'. Wed May 02 17:58:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly160gln' Tue Jun 30 15:37:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly160Gln' Q746J7:p.Gly192Gln G192Q mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain G Q This feature has been corrected as a result of our quality control procedures. The original label was 'G192Q'. Wed May 02 18:10:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly192gln' Tue Jun 30 18:13:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly192Gln' ecolx Escherichia coli direct interaction direct interaction