DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism. Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism. Nature chemical biology 2015 imex curation Yu H., Takeuchi M., LeBarron J., Kantharia J., London E., Bakker H., Haltiwanger RS., Li H., Takeuchi H. Only protein-protein interactions in vitro In vitro glycosylase assay glycosylase assay predetermined predetermined participant predetermined Yu H., Takeuchi M., LeBarron J., Kantharia J., London E., Bakker H., Haltiwanger RS., Li H., Takeuchi H. DIP original experiment: DIP-268146X Nature chemical biology 2015 IMEx Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism. Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism. Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism. Nature chemical biology 2015 imex curation Yu H., Takeuchi M., LeBarron J., Kantharia J., London E., Bakker H., Haltiwanger RS., Li H., Takeuchi H. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Yu H., Takeuchi M., LeBarron J., Kantharia J., London E., Bakker H., Haltiwanger RS., Li H., Takeuchi H. DIP original experiment: DIP-268145X Nature chemical biology 2015 IMEx Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism. Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism. Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism. Nature chemical biology 2015 imex curation Yu H., Takeuchi M., LeBarron J., Kantharia J., London E., Bakker H., Haltiwanger RS., Li H., Takeuchi H. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight silver stain molecular weight estimation by silver staining weight silver stain Yu H., Takeuchi M., LeBarron J., Kantharia J., London E., Bakker H., Haltiwanger RS., Li H., Takeuchi H. DIP original experiment: DIP-268144X Nature chemical biology 2015 IMEx Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism. fa9_human Coagulation factor IX F9 Christmas factor Plasma thromboplastin component protein protein human Homo sapiens Human MQRVNMIMAESPGLITICLLGYLLSAECTVFLDHENANKILNRPKRYNSGKLEEFVQGNLERECMEEKCSFEEAREVFENTERTTEFWKQYVDGDQCESNPCLNGGSCKDDINSYECWCPFGFEGKNCELDVTCNIKNGRCEQFCKNSADNKVVCSCTEGYRLAENQKSCEPAVPFPCGRVSVSQTSKLTRAETVFPDVDYVNSTEAETILDNITQSTQSFNDFTRVVGGEDAKPGQFPWQVVLNGKVDAFCGGSIVNEKWIVTAAHCVETGVKITVVAGEHNIEETEHTEQKRNVIRIIPHHNYNAAINKYNHDIALLELDEPLVLNSYVTPICIADKEYTNIFLKFGSGYVSGWGRVFHKGRSALVLQYLRVPLVDRATCLRSTKFTIYNNMFCAGFHEGGRDSCQGDSGGPHVTEVEGTSFLTGIISWGEECAMKGKYGIYTKVSRYVNWIKEKTKLT DIP protein P00740 original sequence version: 167 DIP protein P00740-1 original sequence version: 218 DIP protein P00740 original sequence version: 173 C4720C1234477EF5 xxlt1_mouse Xyloside xylosyltransferase 1 Xxylt1 UDP-xylose:alpha-xyloside alpha-1,3-xylosyltransferase protein protein mouse Mus musculus Mouse MGLLRGGAACARAMARLGALRSHYCALLLAAALAVCAFYYLGSGRETFSSATKRLKEARAGAAAPTPPAPELARGSAAPASGAKAKSLEGGVVVPVDYHLLMMFTKAEHNAPLQAKARVALSSLLRLAKFEAHEVLNLHFVSEEASREVAKALLRELLPPAAGFKCKVIFHDVAVLTDKLFPVVEAMQKYFSAGSGTYYSDSIFFLSVAMHQIMPKEIPRIIQLDLDLKYKTNIRELFEEFDNFLPGAVIGIAREMQPVYRHTFWQFRHENPKTRVGDPPPEGLPGFNSGVMLLNLEAMRQSPLYSHLLEPSWVQQLADKYHFRGHLGDQDFFTMIGMEHPELFHVLDCTWNRQLCTWWRDHGYSDVFQAYFRCEGHVKIYHGNCNTPIPED DIP protein Q3U4G3 original sequence version: 71 f9-xxylt1-1 1 4 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 92-130 Region 92-130 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 enzyme enzyme unspecified role unspecified role Region 87-392 Region 87-392 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q3U4G3:p.Gln330Ala Q330A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Q A This feature has been corrected as a result of our quality control procedures. The original label was 'Q330A'. Wed May 02 18:53:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln330ala' Tue Jun 30 17:28:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln330Ala' Q3U4G3:p.Trp359Ala W359A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W359A'. Wed May 02 16:56:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp359ala' Tue Jun 30 16:47:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp359Ala' Q3U4G3:p.Glu255Ala E255A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E255A'. Wed May 02 15:49:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu255ala' Tue Jun 30 19:59:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu255Ala' Q3U4G3:p.His326Ala H326A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain H A This feature has been corrected as a result of our quality control procedures. The original label was 'H326A'. Wed May 02 16:46:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his326ala' Tue Jun 30 15:52:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His326Ala' Q3U4G3:p.Trp358Ala W358A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W358A'. Wed May 02 17:38:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp358ala' Tue Jun 30 20:14:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp358Ala' Q3U4G3:p.Asn384Ala N384A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain N A This feature has been corrected as a result of our quality control procedures. The original label was 'N384A'. Wed May 02 20:21:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn384ala' Tue Jun 30 16:31:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn384Ala' Q3U4G3:p.Gln257Ala Q257A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Q A This feature has been corrected as a result of our quality control procedures. The original label was 'Q257A'. Wed May 02 18:07:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln257ala' Tue Jun 30 19:55:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln257Ala' Q3U4G3:p.Ser289Ala S289A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A This feature has been corrected as a result of our quality control procedures. The original label was 'S289A'. Wed May 02 16:03:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser289ala' Tue Jun 30 17:06:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser289Ala' Q3U4G3:p.Arg324Ser R324S mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R S This feature has been corrected as a result of our quality control procedures. The original label was 'R324S'. Wed May 02 17:51:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg324ser' Tue Jun 30 18:25:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg324Ser' Q3U4G3:p.Gly325Ser G325S mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain G S This feature has been corrected as a result of our quality control procedures. The original label was 'G325S'. Wed May 02 19:48:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly325ser' Tue Jun 30 19:29:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly325Ser' human Homo sapiens Human glycosylation glycosylation reaction glycosylation f9-xxylt1-2 3 4 unspecified role unspecified role unspecified role unspecified role Region 92-130 Region 92-130 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role Region 87-392 Region 87-392 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human direct interaction direct interaction f9-xxylt1-3 2 4 unspecified role unspecified role unspecified role unspecified role Region 92-130 Region 92-130 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role Region 87-392 Region 87-392 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human direct interaction direct interaction