DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural Basis for a Novel Interaction between the NS1 Protein Derived from the 1918 Influenza Virus and RIG-I. Structural Basis for a Novel Interaction between the NS1 Protein Derived from the 1918 Influenza Virus and RIG-I. Structure (London, England : 1993) 2015 imex curation Jureka AS., Kleinpeter AB., Cornilescu G., Cornilescu CC., Petit CM. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Jureka AS., Kleinpeter AB., Cornilescu G., Cornilescu CC., Petit CM. DIP original experiment: DIP-267293X Structure (London, England : 1993) 2015 IMEx Structural Basis for a Novel Interaction between the NS1 Protein Derived from the 1918 Influenza Virus and RIG-I. Structural Basis for a Novel Interaction between the NS1 Protein Derived from the 1918 Influenza Virus and RIG-I. Structural Basis for a Novel Interaction between the NS1 Protein Derived from the 1918 Influenza Virus and RIG-I. Structure (London, England : 1993) 2015 imex curation Jureka AS., Kleinpeter AB., Cornilescu G., Cornilescu CC., Petit CM. Only protein-protein interactions ecolx Escherichia coli nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Jureka AS., Kleinpeter AB., Cornilescu G., Cornilescu CC., Petit CM. DIP original experiment: DIP-267292X Structure (London, England : 1993) 2015 IMEx Structural Basis for a Novel Interaction between the NS1 Protein Derived from the 1918 Influenza Virus and RIG-I. Structural Basis for a Novel Interaction between the NS1 Protein Derived from the 1918 Influenza Virus and RIG-I. Structural Basis for a Novel Interaction between the NS1 Protein Derived from the 1918 Influenza Virus and RIG-I. Structure (London, England : 1993) 2015 imex curation Jureka AS., Kleinpeter AB., Cornilescu G., Cornilescu CC., Petit CM. Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Jureka AS., Kleinpeter AB., Cornilescu G., Cornilescu CC., Petit CM. DIP original experiment: DIP-267294X Structure (London, England : 1993) 2015 IMEx Structural Basis for a Novel Interaction between the NS1 Protein Derived from the 1918 Influenza Virus and RIG-I. o95786-1 Antiviral innate immune response receptor RIG-I Probable ATP-dependent RNA helicase DDX58 DDX58 DEAD box protein 58 RIG-I-like receptor 1 Retinoic acid-inducible gene 1 protein Retinoic acid-inducible gene I protein protein protein human Homo sapiens Human MTTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAEKNNKGPMEAATLFLKFLLELQEEGWFRGFLDALDHAGYSGLYEAIESWDFKKIEKLEEYRLLLKRLQPEFKTRIIPTDIISDLSECLINQECEEILQICSTKGMMAGAEKLVECLLRSDKENWPKTLKLALEKERNKFSELWIVEKGIKDVETEDLEDKMETSDIQIFYQEDPECQNLSENSCPPSEVSDTNLYSPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIATVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICKDLENLSQIQNREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYNDALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESVSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGNPKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPKPVPDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFDPAEMSK DIP protein O95786-1 original sequence version: 126 DIP protein O95786-1 original sequence version: 118 DIP protein O95786 original sequence version: 63 DIP protein O95786 original sequence version: 116 DIP protein O95786-1 original sequence version: 116 DIP protein O95786 original sequence version: 105 DIP protein O95786 original sequence version: 80 DIP protein O95786-1 original sequence version: 78 DIP protein O95786 original sequence version: 102 DIP protein O95786-1 original sequence version: 131 DIP protein O95786 original sequence version: 134 DIP protein O95786 original sequence version: 135 DIP protein O95786-1 original sequence version: 120 DIP protein O95786 original sequence version: 120 ns1_i18a0 Non-structural protein 1 NS NS1A protein protein i18a0 Influenza A virus (strain A/Brevig Mission/1/1918 H1N1) MDSNTVSSFQVDCFLWHVRKRFADQELGDAPFLDRLRRDQKSLRGRGSTLGLDIETATRAGKQIVERILKEESDEALKMTIASVPASRYLTDMTLEEMSRDWFMLMPKQKVAGSLCIRMDQAIMDKNIILKANFSVIFDRLETLILLRAFTEEGAIVGEISPLPSLPGHTDEDVKNAVGVLIGGLEWNDNTVRVSETLQRFAWRSSNENGRPPLPPKQKRKMARTIKSEV DIP protein Q99AU3 original sequence version: 64 B3257EA296ADA840 ns-8 2 5 unspecified role unspecified role unspecified role unspecified role Region 1-73 Region 1-73 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ddx58-ns-1 1 4 unspecified role unspecified role unspecified role unspecified role Region 90-188 Region 90-188 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role Region 1-73 Region 1-73 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q99AU3:p.His17Ala H17A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain H A This feature has been corrected as a result of our quality control procedures. The original label was 'H17A'. Wed May 02 19:37:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his17ala' Tue Jun 30 18:00:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His17Ala' ecolx Escherichia coli direct interaction direct interaction ddx58-ns-2 3 4 unspecified role unspecified role unspecified role unspecified role Region 90-188 Region 90-188 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role Region 1-73 Region 1-73 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction