DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Structure (London, England : 1993) 2015 imex curation Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. DIP original experiment: DIP-267284X Structure (London, England : 1993) 2015 IMEx Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Structure (London, England : 1993) 2015 imex curation Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. DIP original experiment: DIP-267282X Structure (London, England : 1993) 2015 IMEx Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Structure (London, England : 1993) 2015 imex curation Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. DIP original experiment: DIP-267287X Structure (London, England : 1993) 2015 IMEx Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Structure (London, England : 1993) 2015 imex curation Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. DIP original experiment: DIP-267283X Structure (London, England : 1993) 2015 IMEx Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Structure (London, England : 1993) 2015 imex curation Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. DIP original experiment: DIP-267285X Structure (London, England : 1993) 2015 IMEx Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. Structure (London, England : 1993) 2015 imex curation Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Xiao S., Brannon MK., Zhao X., Fread KI., Ellena JF., Bushweller JH., Finkielstein CV., Armstrong GS., Capelluto DG. DIP original experiment: DIP-267286X Structure (London, England : 1993) 2015 IMEx Tom1 Modulates Binding of Tollip to Phosphatidylinositol 3-Phosphate via a Coupled Folding and Binding Mechanism. tom1_human Target of Myb protein 1 TOM1 protein protein human Homo sapiens Human MDFLLGNPFSSPVGQRIEKATDGSLQSEDWALNMEICDIINETEEGPKDALRAVKKRIVGNKNFHEVMLALTVLETCVKNCGHRFHVLVASQDFVESVLVRTILPKNNPPTIVHDKVLNLIQSWADAFRSSPDLTGVVTIYEDLRRKGLEFPMTDLDMLSPIHTPQRTVFNSETQSGQDSVGTDSSQQEDSGQHAAPLPAPPILSGDTPIAPTPEQIGKLRSELEMVSGNVRVMSEMLTELVPTQAEPADLELLQELNRTCRAMQQRVLELIPQIANEQLTEELLIVNDNLNNVFLRHERFERFRTGQTTKAPSEAEPAADLIDMGPDPAATGNLSSQLAGMNLGSSSVRAGLQSLEASGRLEDEFDMFALTRGSSLADQRKEVKYEAPQATDGLAGALDARQQSTGAIPVTQACLMEDIEQWLSTDVGNDAEEPKGVTSEEFDKFLEERAKAADRLPNLSSPSAEGPPGPPSGPAPRKKTQEKDDDMLFAL DIP protein O60784 original sequence version: 148 DIP protein O60784 original sequence version: 146 C7D77B5669476242 q9h0e2-1 Toll-interacting protein TOLLIP protein protein human Homo sapiens Human MATTVSTQRGPVYIGELPQDFLRITPTQQQRQVQLDAQAAQQLQYGGAVGTVGRLNITVVQAKLAKNYGMTRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIHCTVPPGVDSFYLEIFDERAFSMDDRIAWTHITIPESLRQGKVEDKWYSLSGRQGDDKEGMINLVMSYALLPAAMVMPPQPVVLMPTVYQQGVGYVPITGMPAVCSPGMVPVALPPAAVNAQPRCSEEDLKAIQDMFPNMDQEVIRSVLEAQRGNKDAAINSLLQMGEEP DIP protein Q9H0E2-1 original sequence version: 136 ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG tollip-tom1-4 2 8 unspecified role unspecified role unspecified role unspecified role Region 1-53 Region 1-53 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Region 54-182 Region 54-182 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 215-309 Region 215-309 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction tom1-ub-1 5 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 215-309 Region 215-309 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction tollip-tom1-5 3 8 unspecified role unspecified role unspecified role unspecified role Region 1-53 Region 1-53 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 215-309 Region 215-309 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction tom1-tollip-1 4 8 unspecified role unspecified role prey prey Region 1-53 Region 1-53 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role bait bait Region 215-309 Region 215-309 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain O60784:p.Glu223Ala E223A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E223A'. Wed May 02 19:46:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu223ala' Tue Jun 30 18:21:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu223Ala' O60784:p.Met226Ala M226A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain M A This feature has been corrected as a result of our quality control procedures. The original label was 'M226A'. Wed May 02 19:48:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met226ala' Tue Jun 30 18:53:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met226Ala' O60784:p.Val227Ala V227A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'V227A'. Wed May 02 18:22:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val227ala' Tue Jun 30 15:43:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val227Ala' O60784:p.Asn230Ala N230A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain N A This feature has been corrected as a result of our quality control procedures. The original label was 'N230A'. Wed May 02 17:32:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn230ala' Tue Jun 30 18:58:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn230Ala' O60784:p.Leu257Ala L257A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L257A'. Wed May 02 19:33:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu257ala' Tue Jun 30 16:14:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu257Ala' O60784:p.Cys261Ser C261S mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain C S This feature has been corrected as a result of our quality control procedures. The original label was 'C261S'. Wed May 02 20:16:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys261ser' Tue Jun 30 16:42:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys261Ser' O60784:p.Met264Ala M264A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain M A This feature has been corrected as a result of our quality control procedures. The original label was 'M264A'. Wed May 02 15:39:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met264ala' Tue Jun 30 19:42:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met264Ala' O60784:p.Arg267Ala R267A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R267A'. Wed May 02 16:39:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg267ala' Tue Jun 30 20:16:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg267Ala' ecolx Escherichia coli direct interaction direct interaction tom1-tollip-4 1 8 unspecified role unspecified role prey prey Region 1-53 Region 1-53 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q9H0E2-1:p.[Arg9Ala;Val12Ala] R9A/V12A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'R9A/V12A'. Wed May 02 19:11:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg9ala,val12ala' Tue Jun 30 16:02:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg9Ala;Val12Ala]' Q9H0E2-1:p.[Asp20Ala;Arg23Ala] D20A/R23A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D20A/R23A'. Wed May 02 17:44:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp20ala,arg23ala' Tue Jun 30 19:41:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp20Ala;Arg23Ala]' Q9H0E2-1:p.Phe21Ala F21A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F21A'. Wed May 02 18:18:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe21ala' Tue Jun 30 17:36:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe21Ala' Region 54-182 Region 54-182 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role bait bait Region 215-309 Region 215-309 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ub-tom1-1 6 9 unspecified role unspecified role bait bait ecolx Escherichia coli 7 unspecified role unspecified role prey prey Region 215-309 Region 215-309 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction