DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural Insight into the Mechanism of TFIIH Recognition by the Acidic String of the Nucleotide Excision Repair Factor XPC. Structural Insight into the Mechanism of TFIIH Recognition by the Acidic String of the Nucleotide Excision Repair Factor XPC. Structure (London, England : 1993) 2015 imex curation Okuda M., Kinoshita M., Kakumu E., Sugasawa K., Nishimura Y. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Okuda M., Kinoshita M., Kakumu E., Sugasawa K., Nishimura Y. DIP original experiment: DIP-267253X Structure (London, England : 1993) 2015 IMEx Structural Insight into the Mechanism of TFIIH Recognition by the Acidic String of the Nucleotide Excision Repair Factor XPC. Structural Insight into the Mechanism of TFIIH Recognition by the Acidic String of the Nucleotide Excision Repair Factor XPC. Structural Insight into the Mechanism of TFIIH Recognition by the Acidic String of the Nucleotide Excision Repair Factor XPC. Structure (London, England : 1993) 2015 imex curation Okuda M., Kinoshita M., Kakumu E., Sugasawa K., Nishimura Y. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Okuda M., Kinoshita M., Kakumu E., Sugasawa K., Nishimura Y. DIP original experiment: DIP-267254X Structure (London, England : 1993) 2015 IMEx Structural Insight into the Mechanism of TFIIH Recognition by the Acidic String of the Nucleotide Excision Repair Factor XPC. Structural Insight into the Mechanism of TFIIH Recognition by the Acidic String of the Nucleotide Excision Repair Factor XPC. Structural Insight into the Mechanism of TFIIH Recognition by the Acidic String of the Nucleotide Excision Repair Factor XPC. Structure (London, England : 1993) 2015 imex curation Okuda M., Kinoshita M., Kakumu E., Sugasawa K., Nishimura Y. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Okuda M., Kinoshita M., Kakumu E., Sugasawa K., Nishimura Y. DIP original experiment: DIP-267255X Structure (London, England : 1993) 2015 IMEx Structural Insight into the Mechanism of TFIIH Recognition by the Acidic String of the Nucleotide Excision Repair Factor XPC. Structural Insight into the Mechanism of TFIIH Recognition by the Acidic String of the Nucleotide Excision Repair Factor XPC. Structural Insight into the Mechanism of TFIIH Recognition by the Acidic String of the Nucleotide Excision Repair Factor XPC. Structure (London, England : 1993) 2015 imex curation Okuda M., Kinoshita M., Kakumu E., Sugasawa K., Nishimura Y. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Okuda M., Kinoshita M., Kakumu E., Sugasawa K., Nishimura Y. DIP original experiment: DIP-267256X Structure (London, England : 1993) 2015 IMEx Structural Insight into the Mechanism of TFIIH Recognition by the Acidic String of the Nucleotide Excision Repair Factor XPC. tf2h1_human General transcription factor IIH subunit 1 General transcription factor IIH polypeptide 1 TFIIH basal transcription factor complex p62 subunit Basic transcription factor 2 62 kDa subunit GTF2H1 BTF2 protein protein human Homo sapiens Human MATSSEEVLLIVKKVRQKKQDGALYLMAERIAWAPEGKDRFTISHMYADIKCQKISPEGKAKIQLQLVLHAGDTTNFHFSNESTAVKERDAVKDLLQQLLPKFKRKANKELEEKNRMLQEDPVLFQLYKDLVVSQVISAEEFWANRLNVNATDSSSTSNHKQDVGISAAFLADVRPQTDGCNGLRYNLTSDIIESIFRTYPAVKMKYAENVPHNMTEKEFWTRFFQSHYFHRDRLNTGSKDLFAECAKIDEKGLKTMVSLGVKNPLLDLTALEDKPLDEGYGISSVPSASNSKSIKENSNAAIIKRFNHHSAMVLAAGLRKQEAQNEQTSEPSNMDGNSGDADCFQPAVKRAKLQESIEYEDLGKNNSVKTIALNLKKSDRYYHGPTPIQSLQYATSQDIINSFQSIRQEMEAYTPKLTQVLSSSAASSTITALSPGGALMQGGTQQAINQMVPNDIQSELKHLYVAVGELLRHFWSCFPVNTPFLEEKVVKMKSNLERFQVTKLCPFQEKIRRQYLSTNLVSHIEEMLQTAYNKLHTWQSRRLMKKT DIP protein P32780 original sequence version: 70 DIP protein P32780 original sequence version: 97 DIP protein P32780 original sequence version: 95 DIP protein P32780 original sequence version: 120 DIP protein P32780-1 original sequence version: 151 8F0FCEBBB1FC9C1D ercc3_human General transcription and DNA repair factor IIH helicase subunit XPB ERCC3 XPB XPBC Basic transcription factor 2 89 kDa subunit TFIIH basal transcription factor complex 89 kDa subunit DNA repair protein complementing XP-B cells Xeroderma pigmentosum group B-complementing protein DNA excision repair protein ERCC-3 protein protein human Homo sapiens Human MGKRDRADRDKKKSRKRHYEDEEDDEEDAPGNDPQEAVPSAAGKQVDESGTKVDEYGAKDYRLQMPLKDDHTSRPLWVAPDGHIFLEAFSPVYKYAQDFLVAIAEPVCRPTHVHEYKLTAYSLYAAVSVGLQTSDITEYLRKLSKTGVPDGIMQFIKLCTVSYGKVKLVLKHNRYFVESCHPDVIQHLLQDPVIRECRLRNSEGEATELITETFTSKSAISKTAESSGGPSTSRVTDPQGKSDIPMDLFDFYEQMDKDEEEEEETQTVSFEVKQEMIEELQKRCIHLEYPLLAEYDFRNDSVNPDINIDLKPTAVLRPYQEKSLRKMFGNGRARSGVIVLPCGAGKSLVGVTAACTVRKRCLVLGNSAVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSVAISTYSMLGHTTKRSWEAERVMEWLKTQEWGLMILDEVHTIPAKMFRRVLTIVQAHCKLGLTATLVREDDKIVDLNFLIGPKLYEANWMELQNNGYIAKVQCAEVWCPMSPEFYREYVAIKTKKRILLYTMNPNKFRACQFLIKFHERRNDKIIVFADNVFALKEYAIRLNKPYIYGPTSQGERMQILQNFKHNPKINTIFISKVGDTSFDLPEANVLIQISSHGGSRRQEAQRLGRVLRAKKGMVAEEYNAFFYSLVSQDTQEMAYSTKRQRFLVDQGYSFKVITKLAGMEEEDLAFSTKEEQQQLLQKVLAATDLDAEEEVVAGEFGSRSSQASRRFGTMSSMSGADDTVYMEYHSSRSKAPSKHVHPLFKRFRK DIP protein P19447 original sequence version: 86 DIP protein P19447 original sequence version: 127 DIP protein P19447 original sequence version: 126 DIP protein P19447 original sequence version: 191 DIP protein P19447 original sequence version: 182 F5F4D3A89A7DF826 q01831-1 DNA repair protein complementing XP-C cells XPC XPCC Xeroderma pigmentosum group C-complementing protein p125 protein protein human Homo sapiens Human MARKRAAGGEPRGRELRSQKSKAKSKARREEEEEDAFEDEKPPKKSLLSKVSQGKRKRGCSHPGGSADGPAKKKVAKVTVKSENLKVIKDEALSDGDDLRDFPSDLKKAHHLKRGATMNEDSNEEEEESENDWEEVEELSEPVLGDVRESTAFSRSLLPVKPVEIEIETPEQAKTRERSEKIKLEFETYLRRAMKRFNKGVHEDTHKVHLLCLLANGFYRNNICSQPDLHAIGLSIIPARFTRVLPRDVDTYYLSNLVKWFIGTFTVNAELSASEQDNLQTTLERRFAIYSARDDEELVHIFLLILRALQLLTRLVLSLQPIPLKSATAKGKKPSKERLTADPGGSSETSSQVLENHTKPKTSKGTKQEETFAKGTCRPSAKGKRNKGGRKKRSKPSSSEEDEGPGDKQEKATQRRPHGRERRVASRVSYKEESGSDEAGSGSDFELSSGEASDPSDEDSEPGPPKQRKAPAPQRTKAGSKSASRTHRGSHRKDPSLPAASSSSSSSKRGKKMCSDGEKAEKRSIAGIDQWLEVFCEQEEKWVCVDCVHGVVGQPLTCYKYATKPMTYVVGIDSDGWVRDVTQRYDPVWMTVTRKCRVDAEWWAETLRPYQSPFMDREKKEDLEFQAKHMDQPLPTAIGLYKNHPLYALKRHLLKYEAIYPETAAILGYCRGEAVYSRDCVHTLHSRDTWLKKARVVRLGEVPYKMVKGFSNRARKARLAEPQLREENDLGLFGYWQTEEYQPPVAVDGKVPRNEFGNVYLFLPSMMPIGCVQLNLPNLHRVARKLDIDCVQAITGFDFHGGYSHPVTDGYIVCEEFKDVLLTAWENEQAVIERKEKEKKEKRALGNWKLLAKGLLIRERLKRRYGPKSEAAAPHTDAGGGLSSDEEEGTSSQAEAARILAASWPQNREDEEKQKLKGGPKKTKREKKAAASHLFPFEQL DIP protein Q01831-1 original sequence version: 163 DIP protein Q01831 original sequence version: 123 gtf2h1-xpc-2 3 7 unspecified role unspecified role unspecified role unspecified role Region 124-142 Region 124-142 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q01831-1:p.Glu128Ala E128A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E128A'. Wed May 02 15:12:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu128ala' Tue Jun 30 16:22:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu128Ala' Q01831-1:p.Trp133Phe W133F mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain W F This feature has been corrected as a result of our quality control procedures. The original label was 'W133F'. Wed May 02 17:34:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp133phe' Tue Jun 30 16:34:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp133Phe' Q01831-1:p.Trp133Ala W133A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W133A'. Wed May 02 20:08:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp133ala' Tue Jun 30 19:26:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp133Ala' Q01831-1:p.Val136Ala V136A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'V136A'. Wed May 02 14:48:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val136ala' Tue Jun 30 15:49:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val136Ala' Q01831-1:p.Leu139Ala L139A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L139A'. Wed May 02 20:24:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu139ala' Tue Jun 30 19:53:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu139Ala' pS129 pS129 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain increasing-ptm ptm increasing an interaction increasing-ptm in vitro In vitro 5 unspecified role unspecified role unspecified role unspecified role Region 1-108 Region 1-108 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction gtf2h1-xpc-3 1 7 unspecified role unspecified role unspecified role unspecified role Region 109-156 Region 109-156 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role Region 1-108 Region 1-108 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction gtf2h1-xpc-4 2 7 unspecified role unspecified role unspecified role unspecified role Region 109-156 Region 109-156 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role Region 1-108 Region 1-108 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction xpc-ercc3-1 4 6 unspecified role unspecified role prey prey human Homo sapiens Human 7 unspecified role unspecified role bait bait Q01831-1:p.Val136Ala V136A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'V136A'. Wed May 02 20:22:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val136ala' Tue Jun 30 18:19:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val136Ala' Q01831-1:p.Trp133Ala W133A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W133A'. Wed May 02 17:41:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp133ala' Tue Jun 30 15:57:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp133Ala' Q01831-1:p.[Trp133Ala;Val136Ala] W133A/V136A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain V A certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W133A/V136A'. Wed May 02 18:07:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp133ala,val136ala' Tue Jun 30 19:51:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Trp133Ala;Val136Ala]' Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association