IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Genetic and Physical Interaction Studies Reveal Functional Similarities between ALBINO3 and ALBINO4 in Arabidopsis. Genetic and Physical Interaction Studies Reveal Functional Similarities between ALBINO3 and ALBINO4 in Arabidopsis. Plant physiology 2015 imex curation Trösch R., Töpel M., Flores-Pérez Ú., Jarvis P. request via PAblo see -see pmids: 28684427 and 26586836 The following protein-protein interactions are shown: ALB4 vs. ALB3 This is shown by co-IP (Fig. 5) and BiFC (Fig. 6). ALB4 vs. cpSRP54 (At1g15310), cpSRP43 (At2g47450), VIPP1 (At1g65260) and cpHsc70 (At4g24280). These are shown by co-IP (Fig. 7). A UniProt identifier for ALB4 was inferred from later work published by this group; pmid:28684427 paul.jarvis@plants.ox.ac.uk 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation arath-protoplast Arabidopsis thaliana protoplast Protoplast defined as a membrane-bound cell from which the outer wall has been partially or completely removed. fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Trösch R., Töpel M., Flores-Pérez Ú., Jarvis P. Vectors pSAT4(A)-nEYFP-N1 and pSAT4(A)-cEYFP-N1: EYGP fragments were deduced to be fused to the C terminus of any protein. (Tzfira et al., 2005) pmid:15821977 request via PAblo see -see pmids: 28684427 and 26586836 The following protein-protein interactions are shown: ALB4 vs. ALB3 This is shown by co-IP (Fig. 5) and BiFC (Fig. 6). ALB4 vs. cpSRP54 (At1g15310), cpSRP43 (At2g47450), VIPP1 (At1g65260) and cpHsc70 (At4g24280). These are shown by co-IP (Fig. 7). A UniProt identifier for ALB4 was inferred from later work published by this group; pmid:28684427 paul.jarvis@plants.ox.ac.uk Accepted 2018-JUN-20 AT 12:33 BST AT 12:33 BST by PPORRAS Plant physiology 2015 Only protein-protein interactions imex curation imex curation altered Genetic and Physical Interaction Studies Reveal Functional Similarities between ALBINO3 and ALBINO4 in Arabidopsis. Genetic and Physical Interaction Studies Reveal Functional Similarities between ALBINO3 and ALBINO4 in Arabidopsis. Plant physiology 2015 imex curation Trösch R., Töpel M., Flores-Pérez Ú., Jarvis P. request via PAblo see -see pmids: 28684427 and 26586836 The following protein-protein interactions are shown: ALB4 vs. ALB3 This is shown by co-IP (Fig. 5) and BiFC (Fig. 6). ALB4 vs. cpSRP54 (At1g15310), cpSRP43 (At2g47450), VIPP1 (At1g65260) and cpHsc70 (At4g24280). These are shown by co-IP (Fig. 7). A UniProt identifier for ALB4 was inferred from later work published by this group; pmid:28684427 paul.jarvis@plants.ox.ac.uk 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Trösch R., Töpel M., Flores-Pérez Ú., Jarvis P. request via PAblo see -see pmids: 28684427 and 26586836 The following protein-protein interactions are shown: ALB4 vs. ALB3 This is shown by co-IP (Fig. 5) and BiFC (Fig. 6). ALB4 vs. cpSRP54 (At1g15310), cpSRP43 (At2g47450), VIPP1 (At1g65260) and cpHsc70 (At4g24280). These are shown by co-IP (Fig. 7). Cross-linker dithiobis succinimidyl propionate (DSP) was used followed by anti-flag or anti-ALB4 IP. A UniProt identifier for ALB4 was inferred from later work published by this group; pmid:28684427 paul.jarvis@plants.ox.ac.uk Accepted 2018-JUN-20 AT 12:33 BST AT 12:33 BST by PPORRAS Plant physiology 2015 Only protein-protein interactions imex curation imex curation altered alb4_arath ALBINO3-like protein 1, chloroplastic ALB4 ALB ALB3L1 ART1 Arabidopsis thaliana envelope membrane integrase Protein ALBINA 4 Protein ARTEMIS STIC1 Suppressor of tic40 protein 1 F21J9.16 F21J9_170 At1g24490 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSSTISLKPTHLILSSFSTGKVLQFRRSRFSHTPSSSSSRYRTLVAQLGFRPDSFDFIKDHAENLLYTIADAAVSSSETFESVAGTTTKTTQSNDWFSGIANYMETILKVLKDGLSTVHVPYSYGFAIILLTVLVKAATFPLTKKQVESAMAMKSLTPQIKAIQERYAGDQEKIQLETARLYKLAGINPLAGCLPTLATIPVWIGLYRALSNVADEGLLTEGFFWIPSLAGPTTVAARQNGSGISWLFPFIEGHPPLGWPDTLAYLVLPLLLVFSQYLSIQIMQSSQSNDPAMKSSQAVTKLLPLMIGYFALSVPSGLSLYWLTNNILSTAQQVWLQKYGGAKNPVEKFTNLVTKEDKTQQIEKSFSEPLVQKSVSELKIPREKGGEKVTPECPKPGERFRLLKEQEAKRRREKEERQKAEAALSNQNTDKAHEQDEKSDTAIVAEDDKKTELSAVDETSDGTVAVNGKPSIQKDETTNGTFGIGHDTEQQHSHETEKR 4AC2A1EE3524FB70 alb3_arath Inner membrane protein ALBINO3, chloroplastic ALB3 F8N16.9 At2g28800 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MARVLVSSPSSFFGSPLIKPSSSLRHSGVGGGGTAQFLPYRSNNNKLFTTSTTVRFSLNEIPPFHGLDSSVDIGAIFTRAESLLYTIADAAVVGADSVVTTDSSAVQKSGGWFGFISDAMELVLKILKDGLSAVHVPYAYGFAIILLTIIVKAATYPLTKQQVESTLAMQNLQPKIKAIQQRYAGNQERIQLETSRLYKQAGVNPLAGCLPTLATIPVWIGLYQALSNVANEGLFTEGFFWIPSLGGPTSIAARQSGSGISWLFPFVDGHPPLGWYDTVAYLVLPVLLIASQYVSMEIMKPPQTDDPAQKNTLLVFKFLPLMIGYFALSVPSGLSIYWLTNNVLSTAQQVYLRKLGGAKPNMDENASKIISAGRAKRSIAQPDDAGERFRQLKEQEKRSKKNKAVAKDTVELVEESQSESEEGSDDEEEEAREGALASSTTSKPLPEVGQRRSKRSKRKRTV 2BBF7AF5BA2E6DDE hsp7f_arath Heat shock 70 kDa protein 6, chloroplastic HSP70-6 CPHSC70-1 Chloroplast heat shock protein 70-1 Heat shock protein 70-6 T22A6.110 At4g24280 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MASSAAQIHVLGGIGFASSSSSKRNLNGKGGTFMPRSAFFGTRTGPFSTPTSAFLRMGTRNGGGASRYAVGPVRVVNEKVVGIDLGTTNSAVAAMEGGKPTIVTNAEGQRTTPSVVAYTKSGDRLVGQIAKRQAVVNPENTFFSVKRFIGRKMNEVDEESKQVSYRVVRDENNNVKLECPAINKQFAAEEISAQVLRKLVDDASRFLNDKVTKAVITVPAYFNDSQRTATKDAGRIAGLEVLRIINEPTAASLAYGFDRKANETILVFDLGGGTFDVSVLEVGDGVFEVLSTSGDTHLGGDDFDKRVVDWLAAEFKKDEGIDLLKDKQALQRLTEAAEKAKIELSSLTQTNMSLPFITATADGPKHIETTLTRAKFEELCSDLLDRVRTPVENSLRDAKLSFKDIDEVILVGGSTRIPAVQELVRKVTGKEPNVTVNPDEVVALGAAVQAGVLAGDVSDIVLLDVTPLSIGLETLGGVMTKIIPRNTTLPTSKSEVFSTAADGQTSVEINVLQGEREFVRDNKSLGSFRLDGIPPAPRGVPQIEVKFDIDANGILSVSAVDKGTGKKQDITITGASTLPKDEVDQMVQEAERFAKDDKEKRDAIDTKNQADSVVYQTEKQLKELGEKIPGEVKEKVEAKLQELKDKIGSGSTQEIKDAMAALNQEVMQIGQSLYNQPGAGGPGAGPSPGGEGASSGDSSSSKGGDGDDVIDADFTDSQ 8723B345C601B8A1 sr43c_arath Signal recognition particle 43 kDa protein, chloroplastic CAO T30B22.25 At2g47450 Chromo protein SRP43 CPSRP43 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQKVFLAMDTCALVIHQSLSRIKLSPPKSSSSSSSAFSPESLPIRRIELCFRGAICAAVQRNYEETTSSVEEAEEDDESSSSYGEVNKIIGSRTAGEGAMEYLIEWKDGHSPSWVPSSYIAADVVSEYETPWWTAARKADEQALSQLLEDRDVDAVDENGRTALLFVAGLGSDKCVRLLAEAGADLDHRDMRGGLTALHMAAGYVRPEVVEALVELGADIEVEDERGLTALELAREILKTTPKGNPMQFGRRIGLEKVINVLEGQVFEYAEVDEIVEKRGKGKDVEYLVRWKDGGDCEWVKGVHVAEDVAKDYEDGLEYAVAESVIGKRVGDDGKTIEYLVKWTDMSDATWEPQDNVDSTLVLLYQQQQPMNE DIP protein O22265 original sequence version: 82 DIP protein O22265 original sequence version: 98 F75ED9C7046A1441 vipp1_arath Membrane-associated protein VIPP1, chloroplastic VIPP1 Protein VESICLE-INDUCING PROTEIN IN PLASTIDS 1 T8F5.2 At1g65260 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MALKASPVTGLFPPLRPTASSSPSTSSNRPCSLRILPLRTSFFGNSSGALRVNVLRLACDNRLRCNGHGATMNLFERFSRVVKSYANALISSFEDPEKILEQTVIEMNSDLTKMRQATAQVLASQKQLQNKYKAAQQSSDDWYKRAQLALAKGDEDLAREALKRRKSFADNATALKTQLDQQKGVVDNLVSNTRLLESKIQEAKAKKDTLLARARTAKTATKVQEMIGTVNTSGALSAFEKMEEKVMAMESEADALTQIGTDELEGKFQMLETSSVDDDLADLKKELSGSSKKGELPPGRSTVAASTRYPFKDSEIENELNELRRKANDF 2F4C74BF2994ACF0 sr54c_arath Signal recognition particle 54 kDa protein, chloroplastic FFC At5g03940 F8F6_150 FFC CPSRP54 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEALQFSSVNRVPCTLSCTGNRRIKAAFSSAFTGGTINSASLSSSRNLSTREIWSWVKSKTVVGHGRYRRSQVRAEMFGQLTGGLEAAWSKLKGEEVLTKDNIAEPMRDIRRALLEADVSLPVVRRFVQSVSDQAVGMGVIRGVKPDQQLVKIVHDELVKLMGGEVSELQFAKSGPTVILLAGLQGVGKTTVCAKLACYLKKQGKSCMLIAGDVYRPAAIDQLVILGEQVGVPVYTAGTDVKPADIAKQGLKEAKKNNVDVVIMDTAGRLQIDKGMMDELKDVKKFLNPTEVLLVVDAMTGQEAAALVTTFNVEIGITGAILTKLDGDSRGGAALSVKEVSGKPIKLVGRGERMEDLEPFYPDRMAGRILGMGDVLSFVEKATEVMRQEDAEDLQKKIMSAKFDFNDFLKQTRAVAKMGSMTRVLGMIPGMGKVSPAQIREAEKNLLVMEAMIEVMTPEERERPELLAESPERRKRIAKDSGKTEQQVSALVAQIFQMRVKMKNLMGVMEGGSIPALSGLEDALKAEQKAPPGTARRKRKADSRKKFVESASSKPGPRGFGSGN DIP protein P37107 original sequence version: 105 423F7285FB9063E4 alb3-alb3l1-4 2 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association 5A alb3-alb3l1-5 2 3 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 4 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association 5B I think a few other bands could be considered to be positive - but the text implies not, so not entered? e.g. "OE33" and "PC". alb3-ebfc2_arath-2 1 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell c_terminus nyfp nYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range 3 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell c_terminus cyfp cYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 6-4 alb3-ebfc2_arath-3 1 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell c_terminus nyfp nYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell c_terminus cyfp cYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 6-1 alb3l1-ebfc2_arath-3 1 3 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell c_terminus nyfp nYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range 3 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell c_terminus cyfp cYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 6-2 alb3-alb3l1-6 1 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell c_terminus cyfp cYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range 3 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell c_terminus nyfp nYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 6-3 alb3-alb3l1-7 2 7 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 6 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 7