DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural integration in hypoxia-inducible factors. Structural integration in hypoxia-inducible factors. Nature 2015 imex curation Wu D., Potluri N., Lu J., Kim Y., Rastinejad F. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Wu D., Potluri N., Lu J., Kim Y., Rastinejad F. DIP original experiment: DIP-265965X Nature 2015 IMEx Structural integration in hypoxia-inducible factors. Structural integration in hypoxia-inducible factors. Structural integration in hypoxia-inducible factors. Nature 2015 imex curation Wu D., Potluri N., Lu J., Kim Y., Rastinejad F. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wu D., Potluri N., Lu J., Kim Y., Rastinejad F. DIP original experiment: DIP-265966X Nature 2015 IMEx Structural integration in hypoxia-inducible factors. Structural integration in hypoxia-inducible factors. Structural integration in hypoxia-inducible factors. Nature 2015 imex curation Wu D., Potluri N., Lu J., Kim Y., Rastinejad F. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wu D., Potluri N., Lu J., Kim Y., Rastinejad F. DIP original experiment: DIP-265963X Nature 2015 IMEx Structural integration in hypoxia-inducible factors. Structural integration in hypoxia-inducible factors. Structural integration in hypoxia-inducible factors. Nature 2015 imex curation Wu D., Potluri N., Lu J., Kim Y., Rastinejad F. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Wu D., Potluri N., Lu J., Kim Y., Rastinejad F. DIP original experiment: DIP-265961X Nature 2015 IMEx Structural integration in hypoxia-inducible factors. Structural integration in hypoxia-inducible factors. Structural integration in hypoxia-inducible factors. Nature 2015 imex curation Wu D., Potluri N., Lu J., Kim Y., Rastinejad F. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Wu D., Potluri N., Lu J., Kim Y., Rastinejad F. DIP original experiment: DIP-265960X Nature 2015 IMEx Structural integration in hypoxia-inducible factors. Structural integration in hypoxia-inducible factors. Structural integration in hypoxia-inducible factors. Nature 2015 imex curation Wu D., Potluri N., Lu J., Kim Y., Rastinejad F. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Wu D., Potluri N., Lu J., Kim Y., Rastinejad F. DIP original experiment: DIP-265962X Nature 2015 IMEx Structural integration in hypoxia-inducible factors. Structural integration in hypoxia-inducible factors. Structural integration in hypoxia-inducible factors. Nature 2015 imex curation Wu D., Potluri N., Lu J., Kim Y., Rastinejad F. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Wu D., Potluri N., Lu J., Kim Y., Rastinejad F. DIP original experiment: DIP-265967X Nature 2015 IMEx Structural integration in hypoxia-inducible factors. Structural integration in hypoxia-inducible factors. Structural integration in hypoxia-inducible factors. Nature 2015 imex curation Wu D., Potluri N., Lu J., Kim Y., Rastinejad F. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wu D., Potluri N., Lu J., Kim Y., Rastinejad F. DIP original experiment: DIP-265964X Nature 2015 IMEx Structural integration in hypoxia-inducible factors. Structural integration in hypoxia-inducible factors. Structural integration in hypoxia-inducible factors. Nature 2015 imex curation Wu D., Potluri N., Lu J., Kim Y., Rastinejad F. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Wu D., Potluri N., Lu J., Kim Y., Rastinejad F. DIP original experiment: DIP-265959X Nature 2015 IMEx Structural integration in hypoxia-inducible factors. Structural integration in hypoxia-inducible factors. Structural integration in hypoxia-inducible factors. Nature 2015 imex curation Wu D., Potluri N., Lu J., Kim Y., Rastinejad F. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wu D., Potluri N., Lu J., Kim Y., Rastinejad F. DIP original experiment: DIP-265968X Nature 2015 IMEx Structural integration in hypoxia-inducible factors. arnt_mouse Aryl hydrocarbon receptor nuclear translocator Arnt Dioxin receptor, nuclear translocator Hypoxia-inducible factor 1-beta protein protein mouse Mus musculus Mouse MAATTANPEMTSDVPSLGPTIASGNPGPGIQGGGAVVQRAIKRRSGLDFDDEVEVNTKFLRCDDDQMCNDKERFARSDDEQSSADKERLARENHSEIERRRRNKMTAYITELSDMVPTCSALARKPDKLTILRMAVSHMKSLRGTGNTSTDGSYKPSFLTDQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQPQSEWFGSTLYDQVHPDDVDKLREQLSTSENALTGRVLDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGTSSVDPVSMNRLSFLRNRCRNGLGSVKEGEPHFVVVHCTGYIKAWPPAGVSLPDDDPEAGQGSKFCLVAIGRLQVTSSPNCTDMSNICQPTEFISRHNIEGIFTFVDHRCVATVGYQPQELLGKNIVEFCHPEDQQLLRDSFQQVVKLKGQVLSVMFRFRSKTREWLWMRTSSFTFQNPYSDEIEYIICTNTNVKNSSQEPRPTLSNTIPRSQLGPTANLSLEMGTGQLPSRQQQQQHTELDMVPGRDGLASYNHSQVSVQPVASAGSEHSKPLEKSEGLFAQDRDPRFPEIYPSITADQSKGISSSTVPATQQLFSQGSSFPPNPRPAENFRNSGLTPPVTIVQPSSSAGQILAQISRHSNPAQGSAPTWTSSSRPGFAAQQVPTQATAKTRSSQFGVNNFQTSSSFSAMSLPGAPTASSGTAAYPALPNRGSNFPPETGQTTGQFQARTAEGVGVWPQWQGQQPHHRSSSSEQHVQQTQAQAPSQPEVFQEMLSMLGDQSNTYNNEEFPDLTMFPPFSE DIP protein P53762 original sequence version: 78 DIP protein P53762-1 original sequence version: 143 BDEC0991CD57D402 q61221-1 Hypoxia-inducible factor 1-alpha Hif1a ARNT-interacting protein protein protein mouse Mus musculus Mouse MEGAGGENEKKKMSSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGGLDSEDEMKAQMDCFYLKALDGFVMVLTDDGDMVYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGPVRKGKELNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTESVLKPVESSDMKMTQLFTKVESEDTSCLFDKLKKEPDALTLLAPAAGDTIISLDFGSDDTETEDQQLEDVPLYNDVMFPSSNEKLNINLAMSPLPSSETPKPLRSSADPALNQEVALKLESSPESLGLSFTMPQIQDQPASPSDGSTRQSSPERLLQENVNTPNFSQPNSPSEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESNSPSPPSMSTVTGFQQTQLQKPTITATATTTATTDESKTETKDNKEDIKILIASPSSTQVPQETTTAKASAYSGTHSRTASPDRAGKRVIEQTDKAHPRSLNLSATLNQRNTVPEEELNPKTIASQNAQRKRKMEHDGSLFQAAGIGTLLQQPGDCAPTMSLSWKRVKGFISSEQNGTEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN DIP protein Q61221-1 original sequence version: 173 mint 84D64ECAC2CC753B epas1_mouse Endothelial PAS domain-containing protein 1 Epas1 Hif2a HIF-1-alpha-like factor HIF-related factor Hypoxia-inducible factor 2-alpha protein protein mouse Mus musculus Mouse MTADKEKKRSSSELRKEKSRDAARCRRSKETEVFYELAHELPLPHSVSSHLDKASIMRLAISFLRTHKLLSSVCSENESEAEADQQMDNLYLKALEGFIAVVTQDGDMIFLSENISKFMGLTQVELTGHSIFDFTHPCDHEEIRENLTLKNGSGFGKKSKDVSTERDFFMRMKCTVTNRGRTVNLKSATWKVLHCTGQVRVYNNCPPHSSLCGSKEPLLSCLIIMCEPIQHPSHMDIPLDSKTFLSRHSMDMKFTYCDDRILELIGYHPEELLGRSAYEFYHALDSENMTKSHQNLCTKGQVVSGQYRMLAKHGGYVWLETQGTVIYNPRNLQPQCIMCVNYVLSEIEKNDVVFSMDQTESLFKPHLMAMNSIFDSSDDVAVTEKSNYLFTKLKEEPEELAQLAPTPGDAIISLDFGSQNFDEPSAYGKAILPPGQPWVSGLRSHSAQSESGSLPAFTVPQADTPGNTTPSASSSSSCSTPSSPEDYYSSLENPLKIEVIEKLFAMDTEPRDPGSTQTDFSELDLETLAPYIPMDGEDFQLSPICPEEPLMPESPQPTPQHCFSTMTSIFQPLTPGATHGPFFLDKYPQQLESRKTESEHWPMSSIFFDAGSKGSLSPCCGQASTPLSSMGGRSNTQWPPDPPLHFGPTKWPVGDQSAESLGALPVGSSQLEPPSAPPHVSMFKMRSAKDFGARGPYMMSPAMIALSNKLKLKRQLEYEEQAFQDTSGGDPPGTSSSHLMWKRMKSLMGGTCPLMPDKTISANMAPDEFTQKSMRGLGQPLRHLPPPQPPSTRSSGENAKTGFPPQCYASQFQDYGPPGAQKVSGVASRLLGPSFEPYLLPELTRYDCEVNVPVPGSSTLLQGRDLLRALDQAT DIP protein P97481 original sequence version: 81 DIP protein P97481 original sequence version: 147 arnt-hif1a-3 1 12 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q61221-1:p.Arg170Ala R170A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R170A'. Wed May 02 16:29:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg170ala' Tue Jun 30 18:20:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg170Ala' Q61221-1:p.Val191Asp V191D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain V D This feature has been corrected as a result of our quality control procedures. The original label was 'V191D'. Wed May 02 15:16:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val191asp' Tue Jun 30 18:16:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val191Asp' Q61221-1:p.[Arg170Ala;Val191Asp] R170A/V191D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain V D certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R170A/V191D'. Wed May 02 18:04:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg170ala,val191asp' Tue Jun 30 16:19:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg170Ala;Val191Asp]' human Homo sapiens Human 11 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human physical association physical association hif1a-arnt-3 9 12 unspecified role unspecified role bait bait Region 13-357 Region 13-357 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 11 unspecified role unspecified role prey prey Region 82-464 Region 82-464 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association arnt-hif1a-4 3 12 unspecified role unspecified role unspecified role unspecified role Region 13-357 Region 13-357 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role Region 82-464 Region 82-464 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction epas1-arnt-1 4 13 unspecified role unspecified role bait bait Region 3-361 Region 3-361 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 11 unspecified role unspecified role prey prey Region 82-464 Region 82-464 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association arnt-epas1-6 10 13 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human 11 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range P53762:p.Leu112Glu L112E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L E This feature has been corrected as a result of our quality control procedures. The original label was 'L112E'. Wed May 02 15:12:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu112glu' Tue Jun 30 17:17:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu112Glu' P53762:p.Leu132Glu L132E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L E This feature has been corrected as a result of our quality control procedures. The original label was 'L132E'. Wed May 02 19:04:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu132glu' Tue Jun 30 20:24:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu132Glu' P53762:p.Val136Asp V136D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain V D This feature has been corrected as a result of our quality control procedures. The original label was 'V136D'. Wed May 02 16:21:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val136asp' Tue Jun 30 16:38:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val136Asp' P53762:p.Ile168Asp I168D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain I D This feature has been corrected as a result of our quality control procedures. The original label was 'I168D'. Wed May 02 16:10:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile168asp' Tue Jun 30 17:38:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile168Asp' P53762:p.[Leu112Glu;Leu132Glu] L112E/L132E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L E certain certain sequence position certain certain certain sequence position certain L E This feature has been corrected as a result of our quality control procedures. The original label was 'L112E/L132E'. Wed May 02 20:16:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu112glu,leu132glu' Tue Jun 30 16:41:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu112Glu;Leu132Glu]' P53762:p.[Arg366Ala;Asn448Ala] R366A/N448A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain N A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R366A/N448A'. Wed May 02 18:56:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg366ala,asn448ala' Tue Jun 30 16:21:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg366Ala;Asn448Ala]' P53762:p.Leu167Glu L167E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L E This feature has been corrected as a result of our quality control procedures. The original label was 'L167E'. Wed May 02 16:28:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu167glu' Tue Jun 30 18:07:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu167Glu' P53762:p.Ala171Asp A171D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain A D This feature has been corrected as a result of our quality control procedures. The original label was 'A171D'. Wed May 02 17:11:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala171asp' Tue Jun 30 18:23:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala171Asp' P53762:p.Ile264Asp I264D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain I D This feature has been corrected as a result of our quality control procedures. The original label was 'I264D'. Wed May 02 19:36:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile264asp' Tue Jun 30 17:18:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile264Asp' P53762:p.Val305Asp V305D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain V D This feature has been corrected as a result of our quality control procedures. The original label was 'V305D'. Wed May 02 18:55:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val305asp' Tue Jun 30 17:04:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val305Asp' P53762:p.Arg366Ala R366A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R366A'. Wed May 02 15:24:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg366ala' Tue Jun 30 19:00:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg366Ala' P53762:p.[Leu167Glu;Ala171Asp] L167E/A171D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain A D certain certain sequence position certain certain certain sequence position certain L E This feature has been corrected as a result of our quality control procedures. The original label was 'L167E/A171D'. Wed May 02 20:02:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu167glu,ala171asp' Tue Jun 30 17:43:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu167Glu;Ala171Asp]' P53762:p.[Ile264Asp;Val305Asp] I264D/V305D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain V D certain certain sequence position certain certain certain sequence position certain I D This feature has been corrected as a result of our quality control procedures. The original label was 'I264D/V305D'. Wed May 02 16:09:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile264asp,val305asp' Tue Jun 30 16:13:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile264Asp;Val305Asp]' P53762:p.Arg266Ala R266A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R266A'. Wed May 02 19:33:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg266ala' Tue Jun 30 20:24:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg266Ala' human Homo sapiens Human physical association physical association arnt-epas1-7 8 13 unspecified role unspecified role unspecified role unspecified role Region 3-361 Region 3-361 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role Region 82-464 Region 82-464 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction arnt-hif1a-5 5 12 unspecified role unspecified role unspecified role unspecified role Region 13-357 Region 13-357 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role Region 82-464 Region 82-464 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction arnt-epas1-8 6 13 unspecified role unspecified role unspecified role unspecified role Region 3-361 Region 3-361 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role Region 82-464 Region 82-464 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction arnt-epas1-9 2 13 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P97481:p.Phe169Asp F169D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain F D This feature has been corrected as a result of our quality control procedures. The original label was 'F169D'. Wed May 02 15:57:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe169asp' Tue Jun 30 19:37:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe169Asp' P97481:p.Arg171Ala R171A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R171A'. Wed May 02 16:50:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg171ala' Tue Jun 30 15:40:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg171Ala' P97481:p.Val192Asp V192D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain V D This feature has been corrected as a result of our quality control procedures. The original label was 'V192D'. Wed May 02 15:42:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val192asp' Tue Jun 30 19:17:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val192Asp' P97481:p.His194Ala H194A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain H A This feature has been corrected as a result of our quality control procedures. The original label was 'H194A'. Wed May 02 17:40:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his194ala' Tue Jun 30 17:36:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His194Ala' P97481:p.[Arg171Ala;Val192Asp] R171A/V192D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain V D certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R171A/V192D'. Wed May 02 16:36:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg171ala,val192asp' Tue Jun 30 18:45:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg171Ala;Val192Asp]' human Homo sapiens Human 11 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human physical association physical association arnt-hif1a-6 7 12 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 11 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range P53762:p.Leu112Glu L112E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L E This feature has been corrected as a result of our quality control procedures. The original label was 'L112E'. Wed May 02 17:44:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu112glu' Tue Jun 30 17:24:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu112Glu' P53762:p.Leu132Glu L132E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L E This feature has been corrected as a result of our quality control procedures. The original label was 'L132E'. Wed May 02 17:36:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu132glu' Tue Jun 30 16:47:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu132Glu' P53762:p.Val136Asp V136D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain V D This feature has been corrected as a result of our quality control procedures. The original label was 'V136D'. Wed May 02 15:07:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val136asp' Tue Jun 30 19:44:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val136Asp' P53762:p.Ile168Asp I168D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain I D This feature has been corrected as a result of our quality control procedures. The original label was 'I168D'. Wed May 02 16:40:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile168asp' Tue Jun 30 16:35:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile168Asp' P53762:p.[Leu112Glu;Leu132Glu] L112E/L132E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L E certain certain sequence position certain certain certain sequence position certain L E This feature has been corrected as a result of our quality control procedures. The original label was 'L112E/L132E'. Wed May 02 17:07:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu112glu,leu132glu' Tue Jun 30 16:45:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu112Glu;Leu132Glu]' P53762:p.[Arg366Ala;Asn448Ala] R366A/N448A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain N A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R366A/N448A'. Wed May 02 18:34:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg366ala,asn448ala' Tue Jun 30 18:53:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg366Ala;Asn448Ala]' P53762:p.Leu167Glu L167E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L E This feature has been corrected as a result of our quality control procedures. The original label was 'L167E'. Wed May 02 14:36:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu167glu' Tue Jun 30 19:51:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu167Glu' P53762:p.Ala171Asp A171D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain A D This feature has been corrected as a result of our quality control procedures. The original label was 'A171D'. Wed May 02 17:58:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala171asp' Tue Jun 30 15:46:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala171Asp' P53762:p.Ile264Asp I264D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain I D This feature has been corrected as a result of our quality control procedures. The original label was 'I264D'. Wed May 02 18:16:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile264asp' Tue Jun 30 17:19:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile264Asp' P53762:p.Val305Asp V305D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain V D This feature has been corrected as a result of our quality control procedures. The original label was 'V305D'. Wed May 02 18:44:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val305asp' Tue Jun 30 17:03:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val305Asp' P53762:p.Arg366Ala R366A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R366A'. Wed May 02 15:20:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg366ala' Tue Jun 30 20:08:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg366Ala' P53762:p.[Leu167Glu;Ala171Asp] L167E/A171D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain A D certain certain sequence position certain certain certain sequence position certain L E This feature has been corrected as a result of our quality control procedures. The original label was 'L167E/A171D'. Wed May 02 19:15:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu167glu,ala171asp' Tue Jun 30 16:24:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu167Glu;Ala171Asp]' P53762:p.[Ile264Asp;Val305Asp] I264D/V305D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain V D certain certain sequence position certain certain certain sequence position certain I D This feature has been corrected as a result of our quality control procedures. The original label was 'I264D/V305D'. Wed May 02 15:28:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile264asp,val305asp' Tue Jun 30 20:00:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile264Asp;Val305Asp]' human Homo sapiens Human physical association physical association