DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure of a Bacterial ABC Transporter Involved in the Import of an Acidic Polysaccharide Alginate. Structure of a Bacterial ABC Transporter Involved in the Import of an Acidic Polysaccharide Alginate. Structure (London, England : 1993) 2015 imex curation Maruyama Y., Itoh T., Kaneko A., Nishitani Y., Mikami B., Hashimoto W., Murata K. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Maruyama Y., Itoh T., Kaneko A., Nishitani Y., Mikami B., Hashimoto W., Murata K. DIP original experiment: DIP-267228X Structure (London, England : 1993) 2015 IMEx Structure of a Bacterial ABC Transporter Involved in the Import of an Acidic Polysaccharide Alginate. Structure of a Bacterial ABC Transporter Involved in the Import of an Acidic Polysaccharide Alginate. Structure of a Bacterial ABC Transporter Involved in the Import of an Acidic Polysaccharide Alginate. Structure (London, England : 1993) 2015 imex curation Maruyama Y., Itoh T., Kaneko A., Nishitani Y., Mikami B., Hashimoto W., Murata K. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Maruyama Y., Itoh T., Kaneko A., Nishitani Y., Mikami B., Hashimoto W., Murata K. DIP original experiment: DIP-267227X Structure (London, England : 1993) 2015 IMEx Structure of a Bacterial ABC Transporter Involved in the Import of an Acidic Polysaccharide Alginate. Structure of a Bacterial ABC Transporter Involved in the Import of an Acidic Polysaccharide Alginate. Structure of a Bacterial ABC Transporter Involved in the Import of an Acidic Polysaccharide Alginate. Structure (London, England : 1993) 2015 imex curation Maruyama Y., Itoh T., Kaneko A., Nishitani Y., Mikami B., Hashimoto W., Murata K. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Maruyama Y., Itoh T., Kaneko A., Nishitani Y., Mikami B., Hashimoto W., Murata K. DIP original experiment: DIP-267226X Structure (London, England : 1993) 2015 IMEx Structure of a Bacterial ABC Transporter Involved in the Import of an Acidic Polysaccharide Alginate. Structure of a Bacterial ABC Transporter Involved in the Import of an Acidic Polysaccharide Alginate. Structure of a Bacterial ABC Transporter Involved in the Import of an Acidic Polysaccharide Alginate. Structure (London, England : 1993) 2015 imex curation Maruyama Y., Itoh T., Kaneko A., Nishitani Y., Mikami B., Hashimoto W., Murata K. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Maruyama Y., Itoh T., Kaneko A., Nishitani Y., Mikami B., Hashimoto W., Murata K. DIP original experiment: DIP-267229X Structure (London, England : 1993) 2015 IMEx Structure of a Bacterial ABC Transporter Involved in the Import of an Acidic Polysaccharide Alginate. q9kwt7_sphsx algM2 protein protein sphsx Sphingomonas sp. MLATPFYSRSDRIFGIVNAVLLGIFALCALYPIIYIFSMSISSGAAVTQGRVFLLPVDIDFSAYGRVLHDKLFWTSYANTIFYTVFGVVTSLIFIVPGAYALSKPRIRGRRVFGFIIAFTMWFNAGMIPFFLNMRDLGLLDNRFGILIGFACNAFNIILMRNYFESISASFEEAARMDGANDLQILWKVYIPLAKPALATITLLCAISRWNGYFWAMVLLRAEEKIPLQVYLKKTIVDLNVNEEFAGALLTNSYSMETVVGAIIVMSIIPVIIVYPVVQKYFTKGVMLGGVKE DIP protein Q9KWT7 original sequence version: 53 q9kwt5_sphsx algQ2 protein protein sphsx Sphingomonas sp. MKKMMLSVAAVATLMAFAAPVATAKEATWVTDKPLTLKIHMHFRDKWVWDENWPVAKESFRLTNVKLQSVANKAATNSQEQFNLMMASGDLPDVVGGDNLKDKFIQYGQEGAFVPLNKLIDQYAPHIKAFFKSHPEVERAIKAPDGNIYFIPYVPDGVVARGYFIREDWLKKLNLKPPQNIDELYTVLKAFKEKDPNGNGKADEVPFIDRHPDEVFRLVNFWGARSSGSDNYMDFYIDNGRVKHPWAETAFRDGMKHVAQWYKEGLIDKEIFTRKARAREQMFGGNLGGFTHDWFASTMTFNEGLAKTVPGFKLIPIAPPTNSKGQRWEEDSRQKVRPDGWAITVKNKNPVETIKFFDFYFSRPGRDISNFGVPGVTYDIKNGKAVFKDSVLKSPQPVNNQLYDMGAQIPIGFWQDYDYERQWTTPEAQAGIDMYVKGKYVMPGFEGVNMTREERAIYDKYWADVRTYMYEMGQAWVMGTKDVDKTWDEYQRQLKLRGLYQVLQMMQQAYDRQYKN DIP protein Q9KWT5 original sequence version: 45 q9kwt8_sphsx algM1 protein protein sphsx Sphingomonas sp. MTSATKAQSIPLPAATLDVRSKPLERLWKDIKRDWLLYAMLLPTIIWFLIFLYKPMIGLQMAFKQYSAWKGIAGSPWIGFDHFVTLFQSEQFIRAIKNTLTLSGLSLLFGFPMPILLALMINEVYSKGYRKAVQTIVYLPHFISIVIVAGLVVTFLSPSTGVVNNMLSWIGLDRVYFLTQPEWFRPIYISSNIWKEAGFDSIVYLAAIMSINPALYESAQVDGATRWQMITRITLPCIVPTIAVLLVIRLGHILEVGFEYIILLYQPTTYETADVISTYIYRLGLQGARYDIATAAGIFNAVVALVIVLFANHMSRRITKTGVF DIP protein Q9KWT8 original sequence version: 53 q9kwt9_sphsx algS protein protein sphsx Sphingomonas sp. MVASVSIQNVVKRYDKTTVVHGVSLDIEPGEFVVLVGPSGCGKSTTLRMVAGLEEISGGTIRIDGRVINDLAPKDRDVAMVFQNYALYPHLNVRDNISFGLRLKRTKKSVIDAAVKTAADILGLQPLLERKPSDLSGGQRQRVAMGRAIVRDPKVFLFDEPLSNLDAKLRTQMRAEIKRLHQRLGTTVIYVTHDQVEAMTLADRIVVMRDGLIEQIGKPMDLFLHPANTFVASFIGSPPMNLMPARIAVDSTQHVELNGGNRISLLPRAGTHLAPGQEVVFGIRPEDVTLDGVEGSERAQIKATVDIVEPLGSESILHATVGDHSLVVKVGGLNEVHPGDPVTLHVDLTRVHLFDAQSQASIY DIP protein Q9KWT9 original sequence version: 78 algm1-algm2-1 3 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association algm1-algm2-2 1 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction algm2-algm1-1 2 8 unspecified role unspecified role prey prey ecolx Escherichia coli 5 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 7 unspecified role unspecified role prey prey ecolx Escherichia coli association association algm1-algm2-3 4 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association