DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Biogenesis and structure of a type VI secretion membrane core complex. Biogenesis and structure of a type VI secretion membrane core complex. Nature 2015 imex curation Durand E., Nguyen VS., Zoued A., Logger L., Péhau-Arnaudet G., Aschtgen MS., Spinelli S., Desmyter A., Bardiaux B., Dujeancourt A., Roussel A., Cambillau C., Cascales E., Fronzes R. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Durand E., Nguyen VS., Zoued A., Logger L., Péhau-Arnaudet G., Aschtgen MS., Spinelli S., Desmyter A., Bardiaux B., Dujeancourt A., Roussel A., Cambillau C., Cascales E., Fronzes R. DIP original experiment: DIP-265989X Nature 2015 IMEx Biogenesis and structure of a type VI secretion membrane core complex. Biogenesis and structure of a type VI secretion membrane core complex. Biogenesis and structure of a type VI secretion membrane core complex. Nature 2015 imex curation Durand E., Nguyen VS., Zoued A., Logger L., Péhau-Arnaudet G., Aschtgen MS., Spinelli S., Desmyter A., Bardiaux B., Dujeancourt A., Roussel A., Cambillau C., Cascales E., Fronzes R. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Durand E., Nguyen VS., Zoued A., Logger L., Péhau-Arnaudet G., Aschtgen MS., Spinelli S., Desmyter A., Bardiaux B., Dujeancourt A., Roussel A., Cambillau C., Cascales E., Fronzes R. DIP original experiment: DIP-265987X Nature 2015 IMEx Biogenesis and structure of a type VI secretion membrane core complex. Biogenesis and structure of a type VI secretion membrane core complex. Biogenesis and structure of a type VI secretion membrane core complex. Nature 2015 imex curation Durand E., Nguyen VS., Zoued A., Logger L., Péhau-Arnaudet G., Aschtgen MS., Spinelli S., Desmyter A., Bardiaux B., Dujeancourt A., Roussel A., Cambillau C., Cascales E., Fronzes R. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Durand E., Nguyen VS., Zoued A., Logger L., Péhau-Arnaudet G., Aschtgen MS., Spinelli S., Desmyter A., Bardiaux B., Dujeancourt A., Roussel A., Cambillau C., Cascales E., Fronzes R. DIP original experiment: DIP-265990X Nature 2015 IMEx Biogenesis and structure of a type VI secretion membrane core complex. Biogenesis and structure of a type VI secretion membrane core complex. Biogenesis and structure of a type VI secretion membrane core complex. Nature 2015 imex curation Durand E., Nguyen VS., Zoued A., Logger L., Péhau-Arnaudet G., Aschtgen MS., Spinelli S., Desmyter A., Bardiaux B., Dujeancourt A., Roussel A., Cambillau C., Cascales E., Fronzes R. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Durand E., Nguyen VS., Zoued A., Logger L., Péhau-Arnaudet G., Aschtgen MS., Spinelli S., Desmyter A., Bardiaux B., Dujeancourt A., Roussel A., Cambillau C., Cascales E., Fronzes R. DIP original experiment: DIP-265988X Nature 2015 IMEx Biogenesis and structure of a type VI secretion membrane core complex. Biogenesis and structure of a type VI secretion membrane core complex. Biogenesis and structure of a type VI secretion membrane core complex. Nature 2015 imex curation Durand E., Nguyen VS., Zoued A., Logger L., Péhau-Arnaudet G., Aschtgen MS., Spinelli S., Desmyter A., Bardiaux B., Dujeancourt A., Roussel A., Cambillau C., Cascales E., Fronzes R. Only protein-protein interactions in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Durand E., Nguyen VS., Zoued A., Logger L., Péhau-Arnaudet G., Aschtgen MS., Spinelli S., Desmyter A., Bardiaux B., Dujeancourt A., Roussel A., Cambillau C., Cascales E., Fronzes R. DIP original experiment: DIP-265991X Nature 2015 IMEx Biogenesis and structure of a type VI secretion membrane core complex. Biogenesis and structure of a type VI secretion membrane core complex. Biogenesis and structure of a type VI secretion membrane core complex. Nature 2015 imex curation Durand E., Nguyen VS., Zoued A., Logger L., Péhau-Arnaudet G., Aschtgen MS., Spinelli S., Desmyter A., Bardiaux B., Dujeancourt A., Roussel A., Cambillau C., Cascales E., Fronzes R. Only protein-protein interactions ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Durand E., Nguyen VS., Zoued A., Logger L., Péhau-Arnaudet G., Aschtgen MS., Spinelli S., Desmyter A., Bardiaux B., Dujeancourt A., Roussel A., Cambillau C., Cascales E., Fronzes R. DIP original experiment: DIP-265986X Nature 2015 IMEx Biogenesis and structure of a type VI secretion membrane core complex. d3gu40_eco44 EC042_4527 protein protein eco44 Escherichia coli O44:H18 (strain 042 / EAEC) MNKPVISRAEQIFYPGWLMVSQLRSGQPVEDGKALYRRACQLVKQAREELAEAGFSQKSSDIMLYAFCALLDESVLNREKTDDGWRTWQQDPLQAHFFGTLNAGEELWERIREQLKLPAPDVAVLTCLCRTLQLGFTGQYRSQDDERREDVIRALTARVPAFTFAQDAPVVVRAPGYRAGRTMYWLSWGAGIVTLAGLWCVLSSVLADQVARLTGQG DIP protein D3GU40 original sequence version: 24 DIP protein D3GU40 original sequence version: 29 d3gux5_eco44 EC042_4544 protein protein eco44 Escherichia coli O44:H18 (strain 042 / EAEC) MAIIAGKAGYGLIIALFSLSLSGCGLTQRVADGTVSATKSLFYRQIKTLHLDIRAREAINTSAAGIPLSVVVRIYQLKDNRSFDSADYQALFTGDNEILAGDIIAQKDVWLQPGGSVAVDMPLDDAAKFTGVAAMFLEPDQKKNTWRVVLGRDELEPDTPRLIEVSGNTLTLLPVKDK DIP protein D3GUX5 original sequence version: 24 DIP protein D3GUX5 original sequence version: 25 d3gux0_eco44 EC042_4539 protein protein eco44 Escherichia coli O44:H18 (strain 042 / EAEC) MNKLACLSGRFGRPGIVFIGVAALWWLITRYGAYLGAETRRDQILLLILLSLGVLFVCYLPVMKKYVQELTYRRRARKEQRLPDDEERLAQTPPRYVTVQDIRHTLRRQYGRFWGRKIRILLITGTASEVELLTPGLTEQFWQEEQGTLLLWGGDPSQPENADWLAALRRLRYRPADGIVWVTSGLSETLSAPLTEDALDRVSRAVSSCCERLGWRLPLYVWSLQESPDERGRITQPVGCLLPAECSSDKLKAQLQAMLPGLVAQGIQQICCAPRYYFLLSLAERFRRNIDAVVEPLSVLLRPYRQLLLAGIVFSPATVGGERSVRHRWRMDNRWEALPETVQQLPVRLQPSRTGHNWRRSLAVMAAILMMAQGTGMVVSFLANRSLVAEVQEQIRPAQNQQLSPAERLQALLNLQKSLARLQYREEHGAPWYLRAGMNQNADLLAVVMPLYAQNAHLLLRDAAAAHLEQQLRTFIRLPPDSPQRGKMAKAAYDQLRLYLMLAQPQHMEPAWFSRTLMREWPQRDGVSAVFWQANGPTLLAYYASGIITHPQWKLTADEELVSQSRTLLLRHLGTQNSDAMLYQKMLARVAHQFADMRLTDMTGDTDVSRLFFTDEVVPGMFTRQAWEEAVLPSIDTVINERREEMDWVLTDGRQKAPSPVSPEALRQRLTTRYFADFGNAWLNFLNSLHLRKAQTLSDVTEQLTLMADVRQSPLVALMNTLAVQGCTGQPREAVTDSLVKSARNLLSQEKQPVAVPESRLHGPLATTFGPVLALMDNQNNSADMLNLQTYLTRVTQVRLRLQQIAGSSDPQAMMQLLAQTVLQGKSVDLTDTRDYGSLTAAGLGQEWYGFGQTVFVRPMEQAWQQVLTPAAESLNARWRTAVVDGWNNAFSGRYPFKNVSSDASLPLLAKYLNTDTGRIARFLQNNLSGVLHREGSRWVPDTINTRGLTFNPAFLKAINTLSEIADVAFTTGNAGLHFELRPGTAAGVMQTTLITDNQKLIYVNQMPVWKRFTWPADTEAPGASLSWVSTQAGTRQYADLPGSWGLIRLLEMARRKAAPGVASGWSLSWQAQDGRMLNYTLRTEAGEGPLVLLKLRNFVLPETVFELSGTSAFTGNDEDAGDTVEETD DIP protein D3GUX0 original sequence version: 24 DIP protein D3GUX0 original sequence version: 29 d3gu40_eco44-d3gux0_eco44-1 6 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association d3gux0_eco44-d3gux5_eco44-1 5 9 unspecified role unspecified role unspecified role unspecified role Region 386-1129 Region 386-1129 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction d3gux5_eco44-d3gu40_eco44-1 4 7 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 9 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 8 unspecified role unspecified role bait bait StrepII-tag StrepII-tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli association association d3gux0_eco44-d3gux5_eco44-2 3 9 unspecified role unspecified role unspecified role unspecified role Region 869-1107 Region 869-1107 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction d3gu40_eco44-d3gux0_eco44-2 2 7 unspecified role unspecified role unspecified role unspecified role Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role StrepII-tag StrepII-tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli physical association physical association d3gux0_eco44-d3gu40_eco44-1 1 7 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 9 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 8 unspecified role unspecified role prey prey StrepII-tag StrepII-tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli association association