DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Mechanism of phospho-ubiquitin-induced PARKIN activation. Mechanism of phospho-ubiquitin-induced PARKIN activation. Nature 2015 imex curation Wauer T., Simicek M., Schubert A., Komander D. Only protein-protein interactions in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Wauer T., Simicek M., Schubert A., Komander D. DIP original experiment: DIP-265955X Nature 2015 IMEx Mechanism of phospho-ubiquitin-induced PARKIN activation. Mechanism of phospho-ubiquitin-induced PARKIN activation. Mechanism of phospho-ubiquitin-induced PARKIN activation. Nature 2015 imex curation Wauer T., Simicek M., Schubert A., Komander D. Only protein-protein interactions in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Wauer T., Simicek M., Schubert A., Komander D. DIP original experiment: DIP-265956X Nature 2015 IMEx Mechanism of phospho-ubiquitin-induced PARKIN activation. prkn_human E3 ubiquitin-protein ligase parkin PRKN PARK2 Parkin RBR E3 ubiquitin-protein ligase Parkinson juvenile disease protein 2 protein protein human Homo sapiens Human MIVFVRFNSSHGFPVEVDSDTSIFQLKEVVAKRQGVPADQLRVIFAGKELRNDWTVQNCDLDQQSIVHIVQRPWRKGQEMNATGGDDPRNAAGGCEREPQSLTRVDLSSSVLPGDSVGLAVILHTDSRKDSPPAGSPAGRSIYNSFYVYCKGPCQRVQPGKLRVQCSTCRQATLTLTQGPSCWDDVLIPNRMSGECQSPHCPGTSAEFFFKCGAHPTSDKETSVALHLIATNSRNITCITCTDVRSPVLVFQCNSRHVICLDCFHLYCVTRLNDRQFVHDPQLGYSLPCVAGCPNSLIKELHHFRILGEEQYNRYQQYGAEECVLQMGGVLCPRPGCGAGLLPEPDQRKVTCEGGNGLGCGFAFCRECKEAYHEGECSAVFEASGTTTQAYRVDERAAEQARWEAASKETIKKTTKPCPRCHVPVEKNGGCMHMKCPQPQCRLEWCWNCGCEWNRVCMGDHWFDV DIP protein O60260 original sequence version: 99 DIP protein O60260-1 original sequence version: 145 DIP protein O60260-1 original sequence version: 158 DIP protein O60260 original sequence version: 157 9A8BB802A3FC84C3 e0viu9_pedhc E3 ubiquitin-protein ligase parkin 8230172 Phum_PHUM233570 protein protein pedhc Pediculus humanus subsp. corporis Body louse MSILEWFWNILCGMAQYLTFSKNLTNDNLVNIYVKSNVGGTISVNLDPKSDIKNVKELVAPKLGLEPDDVKIIFAGKELLDSTVIEVLDFFSDILHAVKVNKKIKNVIPDKPLCETLEELHQLNDQKNVESIEESNLKNEGKNKAHFFIYCANPCKKINTGKLRVCCSECKHGAFTVDTDPQSWADVLDKNKITGVCNNVGCEGLYAKFYFKCASHPSQGENDTAVPLNLIKRNHKKIPCLACTDICDPVLVFSCDNRHVTCLECFKNYCGSRLKDRQFLSHPDFGYTLPCPAGCSNSFIEEVHHFRLLTDAQYEQYHRFATEEFILQAGGVLCPQPGCGQGILIDQNCNRVQCSCGYVFCGKCLEGFHLGECLNPTDVPFLSQNCDYPLDPEKLEKARWDEASSTVIKVLTKPCPKCRTSTERAGGCMHMICTRANCGFHWCWVCQGPWERDCMASHWFG DIP protein E0VIU9 original sequence version: 32 ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG park2-ub-3 1 5 unspecified role unspecified role unspecified role unspecified role FIAsH-label FIAsH-label fiash label fiash label FIAsH label 4',5'-bis(1,3,2-dithioarsolan-2-yl)fluorescein FIAsH label n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal ecolx Escherichia coli 3 unspecified role unspecified role unspecified role unspecified role Region 137-465 Region 137-465 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O60260:p.Lys151Glu K151E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K E This feature has been corrected as a result of our quality control procedures. The original label was 'K151E'. Wed May 02 20:04:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys151glu' Tue Jun 30 17:56:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys151Glu' O60260:p.Ala320Arg A320R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain A R This feature has been corrected as a result of our quality control procedures. The original label was 'A320R'. Wed May 02 15:06:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala320arg' Tue Jun 30 16:13:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala320Arg' O60260:p.Gly284Arg G284R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain G R This feature has been corrected as a result of our quality control procedures. The original label was 'G284R'. Wed May 02 15:00:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly284arg' Tue Jun 30 17:59:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly284Arg' O60260:p.His302Ala H302A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain H A This feature has been corrected as a result of our quality control procedures. The original label was 'H302A'. Wed May 02 17:18:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his302ala' Tue Jun 30 17:37:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His302Ala' O60260:p.Arg305Glu R305E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R E This feature has been corrected as a result of our quality control procedures. The original label was 'R305E'. Wed May 02 15:18:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg305glu' Tue Jun 30 19:49:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg305Glu' O60260:p.Tyr312Glu Y312E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y E This feature has been corrected as a result of our quality control procedures. The original label was 'Y312E'. Wed May 02 16:15:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr312glu' Tue Jun 30 17:11:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr312Glu' ecolx Escherichia coli direct interaction direct interaction e0viu9_pedhc-ub-1 2 4 unspecified role unspecified role unspecified role unspecified role Region 140-461 Region 140-461 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role FIAsH-label FIAsH-label fiash label fiash label FIAsH label 4',5'-bis(1,3,2-dithioarsolan-2-yl)fluorescein FIAsH label n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal ecolx Escherichia coli direct interaction direct interaction