DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Interaction between the Linker, Pre-S1, and TRP Domains Determines Folding, Assembly, and Trafficking of TRPV Channels. Interaction between the Linker, Pre-S1, and TRP Domains Determines Folding, Assembly, and Trafficking of TRPV Channels. Structure (London, England : 1993) 2015 imex curation Garcia-Elias A., Berna-Erro A., Rubio-Moscardo F., Pardo-Pastor C., Mrkonjić S., Sepúlveda RV., Vicente R., González-Nilo F., Valverde MA. Only protein-protein interactions human Homo sapiens Human fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Garcia-Elias A., Berna-Erro A., Rubio-Moscardo F., Pardo-Pastor C., Mrkonjić S., Sepúlveda RV., Vicente R., González-Nilo F., Valverde MA. DIP original experiment: DIP-266604X Structure (London, England : 1993) 2015 IMEx Interaction between the Linker, Pre-S1, and TRP Domains Determines Folding, Assembly, and Trafficking of TRPV Channels. Interaction between the Linker, Pre-S1, and TRP Domains Determines Folding, Assembly, and Trafficking of TRPV Channels. Interaction between the Linker, Pre-S1, and TRP Domains Determines Folding, Assembly, and Trafficking of TRPV Channels. Structure (London, England : 1993) 2015 imex curation Garcia-Elias A., Berna-Erro A., Rubio-Moscardo F., Pardo-Pastor C., Mrkonjić S., Sepúlveda RV., Vicente R., González-Nilo F., Valverde MA. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Garcia-Elias A., Berna-Erro A., Rubio-Moscardo F., Pardo-Pastor C., Mrkonjić S., Sepúlveda RV., Vicente R., González-Nilo F., Valverde MA. DIP original experiment: DIP-266603X Structure (London, England : 1993) 2015 IMEx Interaction between the Linker, Pre-S1, and TRP Domains Determines Folding, Assembly, and Trafficking of TRPV Channels. Interaction between the Linker, Pre-S1, and TRP Domains Determines Folding, Assembly, and Trafficking of TRPV Channels. Interaction between the Linker, Pre-S1, and TRP Domains Determines Folding, Assembly, and Trafficking of TRPV Channels. Structure (London, England : 1993) 2015 imex curation Garcia-Elias A., Berna-Erro A., Rubio-Moscardo F., Pardo-Pastor C., Mrkonjić S., Sepúlveda RV., Vicente R., González-Nilo F., Valverde MA. Only protein-protein interactions human Homo sapiens Human fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Garcia-Elias A., Berna-Erro A., Rubio-Moscardo F., Pardo-Pastor C., Mrkonjić S., Sepúlveda RV., Vicente R., González-Nilo F., Valverde MA. DIP original experiment: DIP-266605X Structure (London, England : 1993) 2015 IMEx Interaction between the Linker, Pre-S1, and TRP Domains Determines Folding, Assembly, and Trafficking of TRPV Channels. trpv4_human Transient receptor potential cation channel subfamily V member 4 TRPV4 Osm-9-like TRP channel 4 Vanilloid receptor-like channel 2 Vanilloid receptor-like protein 2 Vanilloid receptor-related osmotically-activated channel Transient receptor potential protein 12 VRL2 VROAC protein protein human Homo sapiens Human MADSSEGPRAGPGEVAELPGDESGTPGGEAFPLSSLANLFEGEDGSLSPSPADASRPAGPGDGRPNLRMKFQGAFRKGVPNPIDLLESTLYESSVVPGPKKAPMDSLFDYGTYRHHSSDNKRWRKKIIEKQPQSPKAPAPQPPPILKVFNRPILFDIVSRGSTADLDGLLPFLLTHKKRLTDEEFREPSTGKTCLPKALLNLSNGRNDTIPVLLDIAERTGNMREFINSPFRDIYYRGQTALHIAIERRCKHYVELLVAQGADVHAQARGRFFQPKDEGGYFYFGELPLSLAACTNQPHIVNYLTENPHKKADMRRQDSRGNTVLHALVAIADNTRENTKFVTKMYDLLLLKCARLFPDSNLEAVLNNDGLSPLMMAAKTGKIGIFQHIIRREVTDEDTRHLSRKFKDWAYGPVYSSLYDLSSLDTCGEEASVLEILVYNSKIENRHEMLAVEPINELLRDKWRKFGAVSFYINVVSYLCAMVIFTLTAYYQPLEGTPPYPYRTTVDYLRLAGEVITLFTGVLFFFTNIKDLFMKKCPGVNSLFIDGSFQLLYFIYSVLVIVSAALYLAGIEAYLAVMVFALVLGWMNALYFTRGLKLTGTYSIMIQKILFKDLFRFLLVYLLFMIGYASALVSLLNPCANMKVCNEDQTNCTVPTYPSCRDSETFSTFLLDLFKLTIGMGDLEMLSSTKYPVVFIILLVTYIILTFVLLLNMLIALMGETVGQVSKESKHIWKLQWATTILDIERSFPVFLRKAFRSGEMVTVGKSSDGTPDRRWCFRVDEVNWSHWNQNLGIINEDPGKNETYQYYGFSHTVGRLRRDRWSSVVPRVVELNKNSNPDEVVVPLDSMGNPRCDGHQQGYPRKWRTDDAPL DIP protein Q9HBA0 original sequence version: 98 DIP protein Q9HBA0 original sequence version: 131 C62056B86C5A6FB6 trpv4-trpv4-6 2 4 unspecified role unspecified role prey prey Q9HBA0:p.[Lys462Ala;Arg464Ala;Lys465Ala] K462A/R464A/K465A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K462A/R464A/K465A'. Wed May 02 18:27:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys462ala,arg464ala,lys465ala' Tue Jun 30 19:08:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys462Ala;Arg464Ala;Lys465Ala]' YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human 4 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human physical association physical association trpv4-1 1 4 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q9HBA0:p.[Lys462Ala;Arg464Ala;Lys465Ala] K462A/R464A/K465A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K462A/R464A/K465A'. Wed May 02 16:06:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys462ala,arg464ala,lys465ala' Tue Jun 30 18:30:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys462Ala;Arg464Ala;Lys465Ala]' Q9HBA0:p.Lys462Gln K462Q mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K Q This feature has been corrected as a result of our quality control procedures. The original label was 'K462Q'. Wed May 02 20:09:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys462gln' Tue Jun 30 19:30:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys462Gln' Q9HBA0:p.Asp425Ala D425A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D425A'. Wed May 02 16:26:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp425ala' Tue Jun 30 16:51:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp425Ala' Q9HBA0:p.Glu745Ala E745A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E745A'. Wed May 02 14:49:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu745ala' Tue Jun 30 19:51:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu745Ala' Q9HBA0:p.[Asp425Ala;Glu745Ala] D425A/E745A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D425A/E745A'. Wed May 02 16:55:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp425ala,glu745ala' Tue Jun 30 19:35:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp425Ala;Glu745Ala]' human Homo sapiens Human 4 unspecified role unspecified role fluorescence donor fluorescence donor CFP-tag CFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q9HBA0:p.[Lys462Ala;Arg464Ala;Lys465Ala] K462A/R464A/K465A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K462A/R464A/K465A'. Wed May 02 15:31:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys462ala,arg464ala,lys465ala' Tue Jun 30 19:02:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys462Ala;Arg464Ala;Lys465Ala]' Q9HBA0:p.Lys462Gln K462Q mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K Q This feature has been corrected as a result of our quality control procedures. The original label was 'K462Q'. Wed May 02 14:53:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys462gln' Tue Jun 30 16:51:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys462Gln' Q9HBA0:p.Asp425Ala D425A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D425A'. Wed May 02 15:46:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp425ala' Tue Jun 30 18:02:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp425Ala' Q9HBA0:p.Glu745Ala E745A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E745A'. Wed May 02 17:01:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu745ala' Tue Jun 30 19:33:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu745Ala' Q9HBA0:p.[Asp425Ala;Glu745Ala] D425A/E745A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D425A/E745A'. Wed May 02 17:59:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp425ala,glu745ala' Tue Jun 30 19:20:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp425Ala;Glu745Ala]' human Homo sapiens Human proximity proximity close-contact colocalization neighbourhood interaction trpv4-5 3 4 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 4 unspecified role unspecified role fluorescence donor fluorescence donor CFP-tag CFP-tag cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human proximity proximity close-contact colocalization neighbourhood interaction