DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env. Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env. Nature structural & molecular biology 2015 imex curation Kwon YD., Pancera M., Acharya P., Georgiev IS., Crooks ET., Gorman J., Joyce MG., Guttman M., Ma X., Narpala S., Soto C., Terry DS., Yang Y., Zhou T., Ahlsen G., Bailer RT., Chambers M., Chuang GY., Doria-Rose NA., Druz A., Hallen MA., Harned A., Kirys T., Louder MK., O'Dell S., Ofek G., Osawa K., Prabhakaran M., Sastry M., Stewart-Jones GB., Stuckey J., Thomas PV., Tittley T., Williams C., Zhang B., Zhao H., Zhou Z., Donald BR., Lee LK., Zolla-Pazner S., Baxa U., Schön A., Freire E., Shapiro L., Lee KK., Arthos J., Munro JB., Blanchard SC., Mothes W., Binley JM., McDermott AB., Mascola JR., Kwong PD. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Kwon YD., Pancera M., Acharya P., Georgiev IS., Crooks ET., Gorman J., Joyce MG., Guttman M., Ma X., Narpala S., Soto C., Terry DS., Yang Y., Zhou T., Ahlsen G., Bailer RT., Chambers M., Chuang GY., Doria-Rose NA., Druz A., Hallen MA., Harned A., Kirys T., Louder MK., O'Dell S., Ofek G., Osawa K., Prabhakaran M., Sastry M., Stewart-Jones GB., Stuckey J., Thomas PV., Tittley T., Williams C., Zhang B., Zhao H., Zhou Z., Donald BR., Lee LK., Zolla-Pazner S., Baxa U., Schön A., Freire E., Shapiro L., Lee KK., Arthos J., Munro JB., Blanchard SC., Mothes W., Binley JM., McDermott AB., Mascola JR., Kwong PD. DIP original experiment: DIP-266087X Nature structural & molecular biology 2015 IMEx Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env. Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env. Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env. Nature structural & molecular biology 2015 imex curation Kwon YD., Pancera M., Acharya P., Georgiev IS., Crooks ET., Gorman J., Joyce MG., Guttman M., Ma X., Narpala S., Soto C., Terry DS., Yang Y., Zhou T., Ahlsen G., Bailer RT., Chambers M., Chuang GY., Doria-Rose NA., Druz A., Hallen MA., Harned A., Kirys T., Louder MK., O'Dell S., Ofek G., Osawa K., Prabhakaran M., Sastry M., Stewart-Jones GB., Stuckey J., Thomas PV., Tittley T., Williams C., Zhang B., Zhao H., Zhou Z., Donald BR., Lee LK., Zolla-Pazner S., Baxa U., Schön A., Freire E., Shapiro L., Lee KK., Arthos J., Munro JB., Blanchard SC., Mothes W., Binley JM., McDermott AB., Mascola JR., Kwong PD. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Kwon YD., Pancera M., Acharya P., Georgiev IS., Crooks ET., Gorman J., Joyce MG., Guttman M., Ma X., Narpala S., Soto C., Terry DS., Yang Y., Zhou T., Ahlsen G., Bailer RT., Chambers M., Chuang GY., Doria-Rose NA., Druz A., Hallen MA., Harned A., Kirys T., Louder MK., O'Dell S., Ofek G., Osawa K., Prabhakaran M., Sastry M., Stewart-Jones GB., Stuckey J., Thomas PV., Tittley T., Williams C., Zhang B., Zhao H., Zhou Z., Donald BR., Lee LK., Zolla-Pazner S., Baxa U., Schön A., Freire E., Shapiro L., Lee KK., Arthos J., Munro JB., Blanchard SC., Mothes W., Binley JM., McDermott AB., Mascola JR., Kwong PD. DIP original experiment: DIP-266086X Nature structural & molecular biology 2015 IMEx Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env. Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env. Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env. Nature structural & molecular biology 2015 imex curation Kwon YD., Pancera M., Acharya P., Georgiev IS., Crooks ET., Gorman J., Joyce MG., Guttman M., Ma X., Narpala S., Soto C., Terry DS., Yang Y., Zhou T., Ahlsen G., Bailer RT., Chambers M., Chuang GY., Doria-Rose NA., Druz A., Hallen MA., Harned A., Kirys T., Louder MK., O'Dell S., Ofek G., Osawa K., Prabhakaran M., Sastry M., Stewart-Jones GB., Stuckey J., Thomas PV., Tittley T., Williams C., Zhang B., Zhao H., Zhou Z., Donald BR., Lee LK., Zolla-Pazner S., Baxa U., Schön A., Freire E., Shapiro L., Lee KK., Arthos J., Munro JB., Blanchard SC., Mothes W., Binley JM., McDermott AB., Mascola JR., Kwong PD. Only protein-protein interactions in vitro In vitro h1-h2 ms mass spectrometry study of hydrogen/deuterium exchange h1-h2 ms predetermined predetermined participant predetermined Kwon YD., Pancera M., Acharya P., Georgiev IS., Crooks ET., Gorman J., Joyce MG., Guttman M., Ma X., Narpala S., Soto C., Terry DS., Yang Y., Zhou T., Ahlsen G., Bailer RT., Chambers M., Chuang GY., Doria-Rose NA., Druz A., Hallen MA., Harned A., Kirys T., Louder MK., O'Dell S., Ofek G., Osawa K., Prabhakaran M., Sastry M., Stewart-Jones GB., Stuckey J., Thomas PV., Tittley T., Williams C., Zhang B., Zhao H., Zhou Z., Donald BR., Lee LK., Zolla-Pazner S., Baxa U., Schön A., Freire E., Shapiro L., Lee KK., Arthos J., Munro JB., Blanchard SC., Mothes W., Binley JM., McDermott AB., Mascola JR., Kwong PD. DIP original experiment: DIP-266085X Nature structural & molecular biology 2015 IMEx Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env. Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env. Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env. Nature structural & molecular biology 2015 imex curation Kwon YD., Pancera M., Acharya P., Georgiev IS., Crooks ET., Gorman J., Joyce MG., Guttman M., Ma X., Narpala S., Soto C., Terry DS., Yang Y., Zhou T., Ahlsen G., Bailer RT., Chambers M., Chuang GY., Doria-Rose NA., Druz A., Hallen MA., Harned A., Kirys T., Louder MK., O'Dell S., Ofek G., Osawa K., Prabhakaran M., Sastry M., Stewart-Jones GB., Stuckey J., Thomas PV., Tittley T., Williams C., Zhang B., Zhao H., Zhou Z., Donald BR., Lee LK., Zolla-Pazner S., Baxa U., Schön A., Freire E., Shapiro L., Lee KK., Arthos J., Munro JB., Blanchard SC., Mothes W., Binley JM., McDermott AB., Mascola JR., Kwong PD. Only protein-protein interactions human Homo sapiens Human molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Kwon YD., Pancera M., Acharya P., Georgiev IS., Crooks ET., Gorman J., Joyce MG., Guttman M., Ma X., Narpala S., Soto C., Terry DS., Yang Y., Zhou T., Ahlsen G., Bailer RT., Chambers M., Chuang GY., Doria-Rose NA., Druz A., Hallen MA., Harned A., Kirys T., Louder MK., O'Dell S., Ofek G., Osawa K., Prabhakaran M., Sastry M., Stewart-Jones GB., Stuckey J., Thomas PV., Tittley T., Williams C., Zhang B., Zhao H., Zhou Z., Donald BR., Lee LK., Zolla-Pazner S., Baxa U., Schön A., Freire E., Shapiro L., Lee KK., Arthos J., Munro JB., Blanchard SC., Mothes W., Binley JM., McDermott AB., Mascola JR., Kwong PD. DIP original experiment: DIP-266082X Nature structural & molecular biology 2015 IMEx Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env. Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env. Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env. Nature structural & molecular biology 2015 imex curation Kwon YD., Pancera M., Acharya P., Georgiev IS., Crooks ET., Gorman J., Joyce MG., Guttman M., Ma X., Narpala S., Soto C., Terry DS., Yang Y., Zhou T., Ahlsen G., Bailer RT., Chambers M., Chuang GY., Doria-Rose NA., Druz A., Hallen MA., Harned A., Kirys T., Louder MK., O'Dell S., Ofek G., Osawa K., Prabhakaran M., Sastry M., Stewart-Jones GB., Stuckey J., Thomas PV., Tittley T., Williams C., Zhang B., Zhao H., Zhou Z., Donald BR., Lee LK., Zolla-Pazner S., Baxa U., Schön A., Freire E., Shapiro L., Lee KK., Arthos J., Munro JB., Blanchard SC., Mothes W., Binley JM., McDermott AB., Mascola JR., Kwong PD. Only protein-protein interactions human Homo sapiens Human tem transmission electron microscopy tem predetermined predetermined participant predetermined Kwon YD., Pancera M., Acharya P., Georgiev IS., Crooks ET., Gorman J., Joyce MG., Guttman M., Ma X., Narpala S., Soto C., Terry DS., Yang Y., Zhou T., Ahlsen G., Bailer RT., Chambers M., Chuang GY., Doria-Rose NA., Druz A., Hallen MA., Harned A., Kirys T., Louder MK., O'Dell S., Ofek G., Osawa K., Prabhakaran M., Sastry M., Stewart-Jones GB., Stuckey J., Thomas PV., Tittley T., Williams C., Zhang B., Zhao H., Zhou Z., Donald BR., Lee LK., Zolla-Pazner S., Baxa U., Schön A., Freire E., Shapiro L., Lee KK., Arthos J., Munro JB., Blanchard SC., Mothes W., Binley JM., McDermott AB., Mascola JR., Kwong PD. DIP original experiment: DIP-266083X Nature structural & molecular biology 2015 IMEx Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env. Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env. Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env. Nature structural & molecular biology 2015 imex curation Kwon YD., Pancera M., Acharya P., Georgiev IS., Crooks ET., Gorman J., Joyce MG., Guttman M., Ma X., Narpala S., Soto C., Terry DS., Yang Y., Zhou T., Ahlsen G., Bailer RT., Chambers M., Chuang GY., Doria-Rose NA., Druz A., Hallen MA., Harned A., Kirys T., Louder MK., O'Dell S., Ofek G., Osawa K., Prabhakaran M., Sastry M., Stewart-Jones GB., Stuckey J., Thomas PV., Tittley T., Williams C., Zhang B., Zhao H., Zhou Z., Donald BR., Lee LK., Zolla-Pazner S., Baxa U., Schön A., Freire E., Shapiro L., Lee KK., Arthos J., Munro JB., Blanchard SC., Mothes W., Binley JM., McDermott AB., Mascola JR., Kwong PD. Only protein-protein interactions human Homo sapiens Human x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Kwon YD., Pancera M., Acharya P., Georgiev IS., Crooks ET., Gorman J., Joyce MG., Guttman M., Ma X., Narpala S., Soto C., Terry DS., Yang Y., Zhou T., Ahlsen G., Bailer RT., Chambers M., Chuang GY., Doria-Rose NA., Druz A., Hallen MA., Harned A., Kirys T., Louder MK., O'Dell S., Ofek G., Osawa K., Prabhakaran M., Sastry M., Stewart-Jones GB., Stuckey J., Thomas PV., Tittley T., Williams C., Zhang B., Zhao H., Zhou Z., Donald BR., Lee LK., Zolla-Pazner S., Baxa U., Schön A., Freire E., Shapiro L., Lee KK., Arthos J., Munro JB., Blanchard SC., Mothes W., Binley JM., McDermott AB., Mascola JR., Kwong PD. DIP original experiment: DIP-266084X Nature structural & molecular biology 2015 IMEx Crystal structure, conformational fixation and entry-related interactions of mature ligand-free HIV-1 Env. q2n0s6_9hiv1 Envelope glycoprotein gp160 Env polyprotein env protein protein 9hiv1 Human immunodeficiency virus 1 MRVMGIQRNCQHLFRWGTMILGMIIICSAAENLWVTVYYGVPVWKDAETTLFCASDAKAYETEKHNVWATHACVPTDPNPQEIHLENVTEEFNMWKNNMVEQMHTDIISLWDQSLKPCVKLTPLCVTLQCTNVTNNITDDMRGELKNCSFNMTTELRDKKQKVYSLFYRLDVVQINENQGNRSNNSNKEYRLINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGTGPCPSVSTVQCTHGIKPVVSTQLLLNGSLAEEEVMIRSENITNNAKNILVQFNTPVQINCTRPNNNTRKSIRIGPGQAFYATGDIIGDIRQAHCTVSKATWNETLGKVVKQLRKHFGNNTIIRFANSSGGDLEVTTHSFNCGGEFFYCNTSGLFNSTWISNTSVQGSNSTGSNDSITLPCRIKQIINMWQRIGQAMYAPPIQGVIRCVSNITGLILTRDGGSTNSTTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRAKRRVVGREKRAVGIGAVFLGFLGAAGSTMGAASMTLTVQARNLLSGIVQQQSNLLRAIEAQQHLLKLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICTTNVPWNSSWSNRNLSEIWDNMTWLQWDKEISNYTQIIYGLLEESQNQQEKNEQDLLALDKWASLWNWFDISNWLWYIKIFIMIVGGLIGLRIVFAVLSVIHRVRQGYSPLSFQTHTPNPRGLDRPERIEEEDGEQDRGRSTRLVSGFLALAWDDLRSLCLFCYHRLRDFILIAARIVELLGHSSLKGLRLGWEGLKYLWNLLAYWGRELKISAINLFDTIAIAVAGWTDRVIEIGQRLCRAFLHIPRRIRQGFERALL DIP protein Q2N0S6 original sequence version: 67 DIP protein Q2N0S6 original sequence version: 70 DIP protein Q2N0S6 original sequence version: 72 DIP protein Q2N0S6 original sequence version: 76 cd4_human T-cell surface glycoprotein CD4 CD4 T-cell surface antigen T4/Leu-3 protein protein human Homo sapiens Human MNRGVPFRHLLLVLQLALLPAATQGKKVVLGKKGDTVELTCTASQKKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDSDTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNIQGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFSFPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLHLTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLMLSLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQPMALIVLGGVAGLLLFIGLGIFFCVRCRHRRRQAERMSQIKRLLSEKKTCQCPHRFQKTCSPI DIP protein P01730 original sequence version: 183 DIP protein P01730 original sequence version: 163 DIP protein P01730 original sequence version: 133 DIP protein P01730 original sequence version: 135 DIP protein P01730 original sequence version: 185 DIP protein P01730 original sequence version: 189 20ED893F9E56D236 env-8 4 7 unspecified role unspecified role unspecified role unspecified role Region 30-504 Region 30-504 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human direct interaction direct interaction cd4-env-8 3 8 unspecified role unspecified role unspecified role unspecified role Region 26-208 Region 26-208 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human 7 unspecified role unspecified role unspecified role unspecified role Region 30-504 Region 30-504 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human direct interaction direct interaction env-cd4-1 1 8 unspecified role unspecified role prey prey Region 26-208 Region 26-208 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human 7 unspecified role unspecified role bait bait Region 30-504 Region 30-504 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human direct interaction direct interaction env-9 5 7 unspecified role unspecified role unspecified role unspecified role Region 30-504 Region 30-504 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human direct interaction direct interaction cd4-env-9 2 8 unspecified role unspecified role unspecified role unspecified role Region 26-208 Region 26-208 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human 7 unspecified role unspecified role unspecified role unspecified role Region 30-504 Region 30-504 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human direct interaction direct interaction env-10 6 7 unspecified role unspecified role unspecified role unspecified role Region 30-504 Region 30-504 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human direct interaction direct interaction