DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu GTP-Dependent K-Ras Dimerization. GTP-Dependent K-Ras Dimerization. Structure (London, England : 1993) 2015 imex curation Muratcioglu S., Chavan TS., Freed BC., Jang H., Khavrutskii L., Freed RN., Dyba MA., Stefanisko K., Tarasov SG., Gursoy A., Keskin O., Tarasova NI., Gaponenko V., Nussinov R. Only protein-protein interactions in vitro In vitro mst microscale thermophoresis mst predetermined predetermined participant predetermined Muratcioglu S., Chavan TS., Freed BC., Jang H., Khavrutskii L., Freed RN., Dyba MA., Stefanisko K., Tarasov SG., Gursoy A., Keskin O., Tarasova NI., Gaponenko V., Nussinov R. DIP original experiment: DIP-265821X Structure (London, England : 1993) 2015 IMEx GTP-Dependent K-Ras Dimerization. GTP-Dependent K-Ras Dimerization. GTP-Dependent K-Ras Dimerization. Structure (London, England : 1993) 2015 imex curation Muratcioglu S., Chavan TS., Freed BC., Jang H., Khavrutskii L., Freed RN., Dyba MA., Stefanisko K., Tarasov SG., Gursoy A., Keskin O., Tarasova NI., Gaponenko V., Nussinov R. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Muratcioglu S., Chavan TS., Freed BC., Jang H., Khavrutskii L., Freed RN., Dyba MA., Stefanisko K., Tarasov SG., Gursoy A., Keskin O., Tarasova NI., Gaponenko V., Nussinov R. DIP original experiment: DIP-265820X Structure (London, England : 1993) 2015 IMEx GTP-Dependent K-Ras Dimerization. GTP-Dependent K-Ras Dimerization. GTP-Dependent K-Ras Dimerization. Structure (London, England : 1993) 2015 imex curation Muratcioglu S., Chavan TS., Freed BC., Jang H., Khavrutskii L., Freed RN., Dyba MA., Stefanisko K., Tarasov SG., Gursoy A., Keskin O., Tarasova NI., Gaponenko V., Nussinov R. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Muratcioglu S., Chavan TS., Freed BC., Jang H., Khavrutskii L., Freed RN., Dyba MA., Stefanisko K., Tarasov SG., Gursoy A., Keskin O., Tarasova NI., Gaponenko V., Nussinov R. DIP original experiment: DIP-265822X Structure (London, England : 1993) 2015 IMEx GTP-Dependent K-Ras Dimerization. GTP-Dependent K-Ras Dimerization. GTP-Dependent K-Ras Dimerization. Structure (London, England : 1993) 2015 imex curation Muratcioglu S., Chavan TS., Freed BC., Jang H., Khavrutskii L., Freed RN., Dyba MA., Stefanisko K., Tarasov SG., Gursoy A., Keskin O., Tarasova NI., Gaponenko V., Nussinov R. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Muratcioglu S., Chavan TS., Freed BC., Jang H., Khavrutskii L., Freed RN., Dyba MA., Stefanisko K., Tarasov SG., Gursoy A., Keskin O., Tarasova NI., Gaponenko V., Nussinov R. DIP original experiment: DIP-265824X Structure (London, England : 1993) 2015 IMEx GTP-Dependent K-Ras Dimerization. GTP-Dependent K-Ras Dimerization. GTP-Dependent K-Ras Dimerization. Structure (London, England : 1993) 2015 imex curation Muratcioglu S., Chavan TS., Freed BC., Jang H., Khavrutskii L., Freed RN., Dyba MA., Stefanisko K., Tarasov SG., Gursoy A., Keskin O., Tarasova NI., Gaponenko V., Nussinov R. Only protein-protein interactions in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Muratcioglu S., Chavan TS., Freed BC., Jang H., Khavrutskii L., Freed RN., Dyba MA., Stefanisko K., Tarasov SG., Gursoy A., Keskin O., Tarasova NI., Gaponenko V., Nussinov R. DIP original experiment: DIP-265823X Structure (London, England : 1993) 2015 IMEx GTP-Dependent K-Ras Dimerization. GTP-Dependent K-Ras Dimerization. GTP-Dependent K-Ras Dimerization. Structure (London, England : 1993) 2015 imex curation Muratcioglu S., Chavan TS., Freed BC., Jang H., Khavrutskii L., Freed RN., Dyba MA., Stefanisko K., Tarasov SG., Gursoy A., Keskin O., Tarasova NI., Gaponenko V., Nussinov R. Only protein-protein interactions in vitro In vitro dls dynamic light scattering dls predetermined predetermined participant predetermined Muratcioglu S., Chavan TS., Freed BC., Jang H., Khavrutskii L., Freed RN., Dyba MA., Stefanisko K., Tarasov SG., Gursoy A., Keskin O., Tarasova NI., Gaponenko V., Nussinov R. DIP original experiment: DIP-265819X Structure (London, England : 1993) 2015 IMEx GTP-Dependent K-Ras Dimerization. p01116-2 GTPase KRas KRAS KRAS2 RASK2 K-Ras 2 Ki-Ras c-K-ras c-Ki-ras K-Ras4B protein protein human Homo sapiens Human MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM DIP protein P01116-2 original sequence version: 184 DIP protein P01116-2 original sequence version: 140 DIP protein P01116-2 original sequence version: 190 DIP protein P01116-2 original sequence version: 165 Variant in position: 152:V->G (in NS3). Variant in position: 153:D->V (in CFC2 and NS3, exhibits only minor alterations in its in vitro biochemical behavior compared to wild-type protein). Variant in position: 156:F->I (in NS3/CFC2). Variant in position: 156:F->L (found in a patient with Costello syndrome, exhibits an increase in intrinsic and guanine nucleotide exchange factor catalyzed nucleotide exchange in combination with an impaired GTPase-activating protein-stimulated GTP hydrolysis but functional in interaction with effectors). B1B6D189BB259861 kras-1 6 7 unspecified role unspecified role unspecified role unspecified role Region 1-166 Region 1-166 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction kras-kras-1 3 7 unspecified role unspecified role unspecified role unspecified role Region 1-166 Region 1-166 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 1-166 Region 1-166 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Fluorescein-label Fluorescein-label fluor-5-maleimide fluorescein-5-maleimide label fluor-5-maleimide certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction kras-2 4 7 unspecified role unspecified role unspecified role unspecified role Region 1-166 Region 1-166 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction kras-3 2 7 unspecified role unspecified role unspecified role unspecified role Region 1-166 Region 1-166 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction kras-kras-2 1 7 unspecified role unspecified role unspecified role unspecified role Region 1-166 Region 1-166 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 1-166 Region 1-166 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Fluorescein-label Fluorescein-label fluor-5-maleimide fluorescein-5-maleimide label fluor-5-maleimide certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction kras-4 5 7 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Region 1-166 Region 1-166 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain TMR-label TMR-label tetramethyl rhodamine label tetramethyl rhodamine label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role fluorescence donor fluorescence donor Region 1-166 Region 1-166 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Fluorescein-label Fluorescein-label fluor-5-maleimide fluorescein-5-maleimide label fluor-5-maleimide certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction