DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Structure (London, England : 1993) 2015 imex curation Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. DIP original experiment: DIP-265793X Structure (London, England : 1993) 2015 IMEx Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Structure (London, England : 1993) 2015 imex curation Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. DIP original experiment: DIP-265799X Structure (London, England : 1993) 2015 IMEx Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Structure (London, England : 1993) 2015 imex curation Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. DIP original experiment: DIP-265790X Structure (London, England : 1993) 2015 IMEx Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Structure (London, England : 1993) 2015 imex curation Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. DIP original experiment: DIP-265791X Structure (London, England : 1993) 2015 IMEx Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Structure (London, England : 1993) 2015 imex curation Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. DIP original experiment: DIP-265798X Structure (London, England : 1993) 2015 IMEx Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Structure (London, England : 1993) 2015 imex curation Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. DIP original experiment: DIP-265795X Structure (London, England : 1993) 2015 IMEx Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Structure (London, England : 1993) 2015 imex curation Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. DIP original experiment: DIP-265789X Structure (London, England : 1993) 2015 IMEx Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Structure (London, England : 1993) 2015 imex curation Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. DIP original experiment: DIP-265797X Structure (London, England : 1993) 2015 IMEx Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Structure (London, England : 1993) 2015 imex curation Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. DIP original experiment: DIP-265794X Structure (London, England : 1993) 2015 IMEx Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Structure (London, England : 1993) 2015 imex curation Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. DIP original experiment: DIP-265796X Structure (London, England : 1993) 2015 IMEx Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Structure (London, England : 1993) 2015 imex curation Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. DIP original experiment: DIP-265787X Structure (London, England : 1993) 2015 IMEx Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Structure (London, England : 1993) 2015 imex curation Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. DIP original experiment: DIP-265792X Structure (London, England : 1993) 2015 IMEx Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Structure (London, England : 1993) 2015 imex curation Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Lokareddy RK., Hapsari RA., van Rheenen M., Pumroy RA., Bhardwaj A., Steen A., Veenhoff LM., Cingolani G. DIP original experiment: DIP-265788X Structure (London, England : 1993) 2015 IMEx Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. heh2_yeast Inner nuclear membrane protein HEH2 YDR458C D8035.2 HEH2 Helix-extension-helix domain-containing protein 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDHRNLDPKTLKVSQLRRVLVENDVAFPANARKPVLVKLFEEKVRQRLQSSPEASKVRTSIQKVVKSGAKNADRKKTLKSKKLESSSSESKTVKDENVETNKRKREQISTDNEAKMQIQEEKSPKKKRKKRSSKANKPPESPPQSKSDGKATSADLTSELETVEELHKKDSSDDKPRVKELPKPELPNLKVSNEFLAQLNKELASAATENYDHSIKSTDLSSIRIETEEPVGPSTGAETRNESEVMENINLEVQPEVKEAKEELTKISETFDNQDEEDTSRLSSKKNIRSPKGRTRHFIANKTKRGIDIMKPFIAHLFIWLWNGAIFLSIICPILFGLWYREQRIQVGYCGHEKPLKSLAISAFPQTERVDSVLQAYRPNCLECPEHGICSSFMNVECEPGYEPKSSILETYGIIPFPKYCAKDESKEKEVDELVWKVNEYLKKKNAQHECGEGENLFESGETETKLYDIFSHSRPSWESQREFNDHWKNVLEILKKKDDIIWLPLDFETNGKREKSKSNNTNYIYRSTSKKWVTLQCHLEGDIQEYITKYGGSLFITLGVLFLIKKIQSTLDNYVQGEQIIEKLVKEAIDKLKDVKKNKGEEPFLTTVQLRATLLSDIPNIKEQNNLWAQTKEKIMKEQSENIELYLLEENGEIMTCWEWKE 1E11821F2542723E 1E11821F2542723E src1_yeast Inner nuclear membrane protein SRC1 YML033W YML034W SRC1 HEH1 Helix-extension-helix domain-containing protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNSDLEYLEDGFDPNSMKVATLRRILVENNVDFPSNARKNALVGLFDEKVKPQIPQLRKMYLNVRPSDEGIVKMDRPSSSPSIASPRRSRRARREKSASPMAKQFKKNRILDDVSNDDDDDDDDDDDNDKKDDPLIVPSGTDTDEVDDEEDDVITSSSNKSDTNDFQQNSDTRKKRKDPDSDDWSESNSKENKIDNKHLNLLSSDSEIEQDYQKAKKRKTSDLNQEHGNGSAILGKLSVKTPIKNTNRKPVSMDNFNDSLTSSGTENDPFVPNIRHNPKELGTANGTGHSTPLSKLKVSASFADKLPQKEVPSTILVPEVEQQEPSQSERTPSLFSSEGSGSESEAPLLPEITTPGPHQPMGNTSNNVVEMIDTDSSNLVSDEDEVLVPTRIETPQLPTEKDVEKCEARVQELQEEVNEQLEHENGSEFDVKQGSGKVGNRHKFKRALKFLSKSLLALFLFCIFIVIPLLFGLWYREQRLLIGYCGHEVPSHRVSGNSFEFIQKLDNLLQDYRPKCIPCPPNGICYPYLKLKCKPDYKLAPSRLDFLEIIPAQGKCVKDDKKQQLVSEVVEKSLEFLRAKNAQISCGDGKDDIESGMTEDALYQIFNEARAPWIRDDEFEDLWIQVIKDLTEEPEILWRQLSPTDNNIGGNSNNIIKTNDVPRQKRHLPEKFISKTRNFRSTSKKYIGMKCRFEREIYQTYKKFQRPIWLMFLLIVISKVIEIKLKNYYRKKARIEELVTQTMEKLKFQKIKSMSDPKENAYLSIVQLRDIFLSDIVDLKYKNQLWSEVVKYLEHNNSNIKSNLTEIRGEIMKCWEWIGPMELNEPKDSAENKI 214CA064ECE160F4 214CA064ECE160F4 ima1_yeast Importin subunit alpha SRP1 KAP60 YNL189W N1606 Karyopherin subunit alpha Karyopherin-60 Serine-rich RNA polymerase I suppressor protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MDNGTDSSTSKFVPEYRRTNFKNKGRFSADELRRRRDTQQVELRKAKRDEALAKRRNFIPPTDGADSDEEDESSVSADQQFYSQLQQELPQMTQQLNSDDMQEQLSATVKFRQILSREHRPPIDVVIQAGVVPRLVEFMRENQPEMLQLEAAWALTNIASGTSAQTKVVVDADAVPLFIQLLYTGSVEVKEQAIWALGNVAGDSTDYRDYVLQCNAMEPILGLFNSNKPSLIRTATWTLSNLCRGKKPQPDWSVVSQALPTLAKLIYSMDTETLVDACWAISYLSDGPQEAIQAVIDVRIPKRLVELLSHESTLVQTPALRAVGNIVTGNDLQTQVVINAGVLPALRLLLSSPKENIKKEACWTISNITAGNTEQIQAVIDANLIPPLVKLLEVAEYKTKKEACWAISNASSGGLQRPDIIRYLVSQGCIKPLCDLLEIADNRIIEVTLDALENILKMGEADKEARGLNINENADFIEKAGGMEKIFNCQQNENDKIYEKAYKIIETYFGEEEDAVDETMAPQNAGNTFGFGSNVNQQFNFN 8D3A0CB76F2E7C00 8D3A0CB76F2E7C00 nupl_xenla Nucleoplasmin protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MASTVSNTSKLEKPVSLIWGCELNEQDKTFEFKVEDDEEKCEHQLALRTVCLGDKAKDEFNIVEIVTQEEGAEKSVPIATLKPSILPMATMVGIELTPPVTFRLKAGSGPLYISGQHVAMEEDYSWAEEEDEGEAEGEEEEEEEEDQESPPKAVKRPAATKKAGQAKKKKLDKEDESSEEDSPTKKGKGAGRGRKPAAKK DIP protein P05221 original sequence version: 62 DIP protein P05221 original sequence version: 85 Nucleoplasmin is an acidic protein which is able to assemble nucleosomes by binding histones and transferring them to DNA mint A91DD110F2965812 heh2-kap60-2 4 14 unspecified role unspecified role unspecified role unspecified role Region 100-137 Region 100-137 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 16 unspecified role unspecified role unspecified role unspecified role Region 88-530 Region 88-530 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction heh1-kap60-1 11 16 unspecified role unspecified role unspecified role unspecified role Region 88-530 Region 88-530 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role Region 171-221 Region 171-221 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction kap60-heh1-1 5 16 unspecified role unspecified role bait bait Region 88-530 Region 88-530 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 15 unspecified role unspecified role prey prey Region 171-221 Region 171-221 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q03707:p.[Arg176Ala;Lys190Ala] R176A/K190A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R176A/K190A'. Wed May 02 18:50:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg176ala,lys190ala' Tue Jun 30 17:45:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg176Ala;Lys190Ala]' Q03707:p.Arg176Ala R176A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R176A'. Wed May 02 15:17:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg176ala' Tue Jun 30 17:57:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg176Ala' MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction heh2-kap60-3 12 14 unspecified role unspecified role bait bait Region 100-137 Region 100-137 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 16 unspecified role unspecified role prey prey Region 88-530 Region 88-530 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association heh1-kap60-2 13 16 unspecified role unspecified role unspecified role unspecified role Region 88-530 Region 88-530 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role Region 171-221 Region 171-221 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction kap60-nupl_xenla-1 6 17 unspecified role unspecified role unspecified role unspecified role Region 151-172 Region 151-172 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 16 unspecified role unspecified role unspecified role unspecified role Region 88-530 Region 88-530 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction heh1-kap60-3 7 16 unspecified role unspecified role prey prey Region 88-530 Region 88-530 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 15 unspecified role unspecified role bait bait Region 171-221 Region 171-221 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association heh1-kap60-4 1 16 unspecified role unspecified role unspecified role unspecified role Region 88-530 Region 88-530 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role Region 171-221 Region 171-221 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q03707:p.[Arg176Ala;Lys190Ala] R176A/K190A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R176A/K190A'. Wed May 02 15:18:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg176ala,lys190ala' Tue Jun 30 16:32:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg176Ala;Lys190Ala]' Q03707:p.Arg176Ala R176A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R176A'. Wed May 02 15:14:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg176ala' Tue Jun 30 15:58:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg176Ala' Q03707:p.Lys190Ala K190A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K190A'. Wed May 02 15:18:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys190ala' Tue Jun 30 17:24:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys190Ala' ecolx Escherichia coli direct interaction direct interaction kap60-nupl_xenla-2 10 17 unspecified role unspecified role prey prey Region 151-172 Region 151-172 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 16 unspecified role unspecified role bait bait Region 88-530 Region 88-530 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction heh2-kap60-4 2 14 unspecified role unspecified role bait bait Region 97-137 Region 97-137 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 16 unspecified role unspecified role prey prey Region 88-530 Region 88-530 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction heh2-kap60-5 3 14 unspecified role unspecified role unspecified role unspecified role Region 100-137 Region 100-137 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 16 unspecified role unspecified role unspecified role unspecified role Region 88-530 Region 88-530 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction heh2-kap60-6 9 14 unspecified role unspecified role unspecified role unspecified role Region 97-137 Region 97-137 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q03281:p.[Arg103Ala;Lys126Ala] K126A/R103A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'K126A/R103A'. Wed May 02 19:51:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg103ala,lys126ala' Tue Jun 30 20:25:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg103Ala;Lys126Ala]' Q03281:p.Arg103Ala R103A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R103A'. Wed May 02 15:17:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg103ala' Tue Jun 30 15:59:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg103Ala' Q03281:p.Lys126Ala K126A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K126A'. Wed May 02 16:14:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys126ala' Tue Jun 30 18:03:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys126Ala' ecolx Escherichia coli 16 unspecified role unspecified role unspecified role unspecified role Region 88-530 Region 88-530 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction kap60-heh2-1 8 14 unspecified role unspecified role prey prey Region 97-137 Region 97-137 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q03281:p.[Arg103Ala;Lys126Ala] K126A/R103A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'K126A/R103A'. Wed May 02 18:14:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg103ala,lys126ala' Tue Jun 30 17:49:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg103Ala;Lys126Ala]' Q03281:p.Arg103Ala R103A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R103A'. Wed May 02 15:15:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg103ala' Tue Jun 30 20:08:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg103Ala' MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 16 unspecified role unspecified role bait bait Region 88-530 Region 88-530 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction