DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth. Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth. Nature 2015 imex curation Raiborg C., Wenzel EM., Pedersen NM., Olsvik H., Schink KO., Schultz SW., Vietri M., Nisi V., Bucci C., Brech A., Johansen T., Stenmark H. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Raiborg C., Wenzel EM., Pedersen NM., Olsvik H., Schink KO., Schultz SW., Vietri M., Nisi V., Bucci C., Brech A., Johansen T., Stenmark H. DIP original experiment: DIP-265510X Nature 2015 IMEx Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth. Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth. Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth. Nature 2015 imex curation Raiborg C., Wenzel EM., Pedersen NM., Olsvik H., Schink KO., Schultz SW., Vietri M., Nisi V., Bucci C., Brech A., Johansen T., Stenmark H. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Raiborg C., Wenzel EM., Pedersen NM., Olsvik H., Schink KO., Schultz SW., Vietri M., Nisi V., Bucci C., Brech A., Johansen T., Stenmark H. DIP original experiment: DIP-265512X Nature 2015 IMEx Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth. Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth. Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth. Nature 2015 imex curation Raiborg C., Wenzel EM., Pedersen NM., Olsvik H., Schink KO., Schultz SW., Vietri M., Nisi V., Bucci C., Brech A., Johansen T., Stenmark H. Only protein-protein interactions in vitro In vitro pull down pull down autoradiography autoradiography Raiborg C., Wenzel EM., Pedersen NM., Olsvik H., Schink KO., Schultz SW., Vietri M., Nisi V., Bucci C., Brech A., Johansen T., Stenmark H. DIP original experiment: DIP-265511X Nature 2015 IMEx Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth. Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth. Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth. Nature 2015 imex curation Raiborg C., Wenzel EM., Pedersen NM., Olsvik H., Schink KO., Schultz SW., Vietri M., Nisi V., Bucci C., Brech A., Johansen T., Stenmark H. Only protein-protein interactions human Homo sapiens Human pull down pull down anti tag western anti tag western blot anti tag western Raiborg C., Wenzel EM., Pedersen NM., Olsvik H., Schink KO., Schultz SW., Vietri M., Nisi V., Bucci C., Brech A., Johansen T., Stenmark H. DIP original experiment: DIP-265514X Nature 2015 IMEx Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth. Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth. Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth. Nature 2015 imex curation Raiborg C., Wenzel EM., Pedersen NM., Olsvik H., Schink KO., Schultz SW., Vietri M., Nisi V., Bucci C., Brech A., Johansen T., Stenmark H. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Raiborg C., Wenzel EM., Pedersen NM., Olsvik H., Schink KO., Schultz SW., Vietri M., Nisi V., Bucci C., Brech A., Johansen T., Stenmark H. DIP original experiment: DIP-265509X Nature 2015 IMEx Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth. Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth. Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth. Nature 2015 imex curation Raiborg C., Wenzel EM., Pedersen NM., Olsvik H., Schink KO., Schultz SW., Vietri M., Nisi V., Bucci C., Brech A., Johansen T., Stenmark H. Only protein-protein interactions human Homo sapiens Human pull down pull down anti tag western anti tag western blot anti tag western Raiborg C., Wenzel EM., Pedersen NM., Olsvik H., Schink KO., Schultz SW., Vietri M., Nisi V., Bucci C., Brech A., Johansen T., Stenmark H. DIP original experiment: DIP-265515X Nature 2015 IMEx Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth. Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth. Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth. Nature 2015 imex curation Raiborg C., Wenzel EM., Pedersen NM., Olsvik H., Schink KO., Schultz SW., Vietri M., Nisi V., Bucci C., Brech A., Johansen T., Stenmark H. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Raiborg C., Wenzel EM., Pedersen NM., Olsvik H., Schink KO., Schultz SW., Vietri M., Nisi V., Bucci C., Brech A., Johansen T., Stenmark H. DIP original experiment: DIP-265513X Nature 2015 IMEx Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth. fyco1_human FYVE and coiled-coil domain-containing protein 1 FYCO1 ZFYVE7 Zinc finger FYVE domain-containing protein 7 protein protein human Homo sapiens Human MASTNAESQLQRIIRDLQDAVTELSKEFQEAGEPITDDSTSLHKFSYKLEYLLQFDQKEKATLLGNKKDYWDYFCACLAKVKGANDGIRFVKSISELRTSLGKGRAFIRYSLVHQRLADTLQQCFMNTKVTSDWYYARSPFLQPKLSSDIVGQLYELTEVQFDLASRGFDLDAAWPTFARRTLTTGSSAYLWKPPSRSSSMSSLVSSYLQTQEMVSNFDLNSPLNNEALEGFDEMRLELDQLEVREKQLRERMQQLDRENQELRAAVSQQGEQLQTERERGRTAAEDNVRLTCLVAELQKQWEVTQATQNTVKELQTCLQGLELGAAEKEEDYHTALRRLESMLQPLAQELEATRDSLDKKNQHLASFPGWLAMAQQKADTASDTKGRQEPIPSDAAQEMQELGEKLQALERERTKVEEVNRQQSAQLEQLVKELQLKEDARASLERLVKEMAPLQEELSGKGQEADQLWRRLQELLAHTSSWEEELAELRREKKQQQEEKELLEQEVRSLTRQLQFLETQLAQVSQHVSDLEEQKKQLIQDKDHLSQQVGMLERLAGPPGPELPVAGEKNEALVPVNSSLQEAWGKPEEEQRGLQEAQLDDTKVQEGSQEEELRQANRELEKELQNVVGRNQLLEGKLQALQADYQALQQRESAIQGSLASLEAEQASIRHLGDQMEASLLAVRKAKEAMKAQMAEKEAILQSKEGECQQLREEVEQCQQLAEARHRELRALESQCQQQTQLIEVLTAEKGQQGVGPPTDNEARELAAQLALSQAQLEVHQGEVQRLQAQVVDLQAKMRAALDDQDKVQSQLSMAEAVLREHKTLVQQLKEQNEALNRAHVQELLQCSEREGALQEERADEAQQREEELRALQEELSQAKCSSEEAQLEHAELQEQLHRANTDTAELGIQVCALTVEKERVEEALACAVQELQDAKEAASREREGLERQVAGLQQEKESLQEKLKAAKAAAGSLPGLQAQLAQAEQRAQSLQEAAHQELNTLKFQLSAEIMDYQSRLKNAGEECKSLRGQLEEQGRQLQAAEEAVEKLKATQADMGEKLSCTSNHLAECQAAMLRKDKEGAALREDLERTQKELEKATTKIQEYYNKLCQEVTNRERNDQKMLADLDDLNRTKKYLEERLIELLRDKDALWQKSDALEFQQKLSAEERWLGDTEANHCLDCKREFSWMVRRHHCRICGRIFCYYCCNNYVLSKHGGKKERCCRACFQKLSEGPGSPDSSGSGTSQGEPSPALSPASPGPQATGGQGANTDYRPPDDAVFDIITDEELCQIQESGSSLPETPTETDSLDPNAAEQDTTSTSLTPEDTEDMPVGQDSEICLLKSGELMIKVPLTVDEIASFGEGSRELFVRSSTYSLIPITVAEAGLTISWVFSSDPKSISFSVVFQEAEDTPLDQCKVLIPTTRCNSHKENIQGQLKVRTPGIYMLIFDNTFSRFVSKKVFYHLTVDRPVIYDGSDFL DIP protein Q9BQS8 original sequence version: 97 DIP protein Q9BQS8-1 original sequence version: 113 11AB671A9F2B3D21 rab7a_canlf Ras-related protein Rab-7a RAB7A RAB7 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MTSRKKVLLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVMVDDRLVTMQIWDTAGQERFQSLGVAFYRGADCCVLVFDVTAPNTFKTLDSWRDEFLIQASPRDPENFPFVVLGNKIDLENRQVATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKNDRAKTSAESCSC DIP protein P18067 original sequence version: 129 mint E3AF33B16A67296D q5t4f4-1 Protrudin ZFYVE27 SPG33 Spastic paraplegia 33 protein Zinc finger FYVE domain-containing protein 27 protein protein human Homo sapiens Human MQTSEREGSGPELSPSVMPEAPLESPPFPTKSPAFDLFNLVLSYKRLEIYLEPLKDAGDGVRYLLRWQMPLCSLLTCLGLNVLFLTLNEGAWYSVGALMISVPALLGYLQEVCRARLPDSELMRRKYHSVRQEDLQRGRLSRPEAVAEVKSFLIQLEAFLSRLCCTCEAAYRVLHWENPVVSSQFYGALLGTVCMLYLLPLCWVLTLLNSTLFLGNVEFFRVVSEYRASLQQRMNPKQEEHAFESPPPPDVGGKDGLMDSTPALTPTEDLTPGSVEEAEEAEPDEEFKDAIEETHLVVLEDDEGAPCPAEDELALQDNGFLSKNEVLRSKVSRLTERLRKRYPTNNFGNCTGCSATFSVLKKRRSCSNCGNSFCSRCCSFKVPKSSMGATAPEAQRETVFVCASCNQTLSK DIP protein Q5T4F4-1 original sequence version: 112 rab7a_human Ras-related protein Rab-7a RAB7A RAB7 protein protein human Homo sapiens Human MTSRKKVLLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVMVDDRLVTMQIWDTAGQERFQSLGVAFYRGADCCVLVFDVTAPNTFKTLDSWRDEFLIQASPRDPENFPFVVLGNKIDLENRQVATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKNDRAKASAESCSC DIP protein P51149 original sequence version: 113 DIP protein P51149 original sequence version: 92 DIP protein P51149 original sequence version: 162 A2AF33B16A672971 klc2_human Kinesin light chain 2 KLC2 protein protein human Homo sapiens Human MAMMVFPREEKLSQDEIVLGTKAVIQGLETLRGEHRALLAPLVAPEAGEAEPGSQERCILLRRSLEAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQQKLQRSEQAVAQLEEEKQHLLFMSQIRKLDEDASPNEEKGDVPKDTLDDLFPNEDEQSPAPSPGGGDVSGQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYRRALEIYATRLGPDDPNVAKTKNNLASCYLKQGKYQDAETLYKEILTRAHEKEFGSVNGDNKPIWMHAEEREESKDKRRDSAPYGEYGSWYKACKVDSPTVNTTLRSLGALYRRQGKLEAAHTLEDCASRNRKQGLDPASQTKVVELLKDGSGRRGDRRSSRDMAGGAGPRSESDLEDVGPTAEWNGDGSGSLRRSGSFGKLRDALRRSSEMLVKKLQGGTPQEPPNPRMKRASSLNFLNKSVEEPTQPGGTGLSDSRTLSSSSMDLSRRSSLVG DIP protein Q9H0B6 original sequence version: 142 5B57ABE4DF6E396E kinh_human Kinesin-1 heavy chain KIF5B KNS KNS1 Ubiquitous kinesin heavy chain Conventional kinesin heavy chain protein protein human Homo sapiens Human MADLAECNIKVMCRFRPLNESEVNRGDKYIAKFQGEDTVVIASKPYAFDRVFQSSTSQEQVYNDCAKKIVKDVLEGYNGTIFAYGQTSSGKTHTMEGKLHDPEGMGIIPRIVQDIFNYIYSMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPYVKGCTERFVCSPDEVMDTIDEGKSNRHVAVTNMNEHSSRSHSIFLINVKQENTQTEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGSTYVPYRDSKMTRILQDSLGGNCRTTIVICCSPSSYNESETKSTLLFGQRAKTIKNTVCVNVELTAEQWKKKYEKEKEKNKILRNTIQWLENELNRWRNGETVPIDEQFDKEKANLEAFTVDKDITLTNDKPATAIGVIGNFTDAERRKCEEEIAKLYKQLDDKDEEINQQSQLVEKLKTQMLDQEELLASTRRDQDNMQAELNRLQAENDASKEEVKEVLQALEELAVNYDQKSQEVEDKTKEYELLSDELNQKSATLASIDAELQKLKEMTNHQKKRAAEMMASLLKDLAEIGIAVGNNDVKQPEGTGMIDEEFTVARLYISKMKSEVKTMVKRCKQLESTQTESNKKMEENEKELAACQLRISQHEAKIKSLTEYLQNVEQKKRQLEESVDALSEELVQLRAQEKVHEMEKEHLNKVQTANEVKQAVEQQIQSHRETHQKQISSLRDEVEAKAKLITDLQDQNQKMMLEQERLRVEHEKLKATDQEKSRKLHELTVMQDRREQARQDLKGLEETVAKELQTLHNLRKLFVQDLATRVKKSAEIDSDDTGGSAAQKQKISFLENNLEQLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALESALKEAKENASRDRKRYQQEVDRIKEAVRSKNMARRGHSAQIAKPIRPGQHPAASPTHPSAIRGGGAFVQNSQPVAVRGGGGKQV DIP protein P33176 original sequence version: 148 DIP protein P33176 original sequence version: 65 DIP protein P33176 original sequence version: 133 DIP protein P33176 original sequence version: 102 DIP protein P33176 original sequence version: 152 A1FE5760C3250C8B zfyve27-rab7a-1 5 9 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 10 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Region 208-274 Region 208-274 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction fyco1-klc2-1 3 12 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 8 unspecified role unspecified role bait bait Region 585-1233 Region 585-1233 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction fyco1-klc2-2 6 12 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 8 unspecified role unspecified role bait bait Region 585-1233 Region 585-1233 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association rab7a-zfyve27-1 7 10 unspecified role unspecified role prey prey Region 208-411 Region 208-411 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 11 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association zfyve27-rab7a-2 1 9 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 10 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Region 208-274 Region 208-274 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction fyco1-kif5b-1 2 13 unspecified role unspecified role prey prey human Homo sapiens Human 8 unspecified role unspecified role bait bait Region 735-773 Region 735-773 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association fyco1-kif5b-2 4 13 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 8 unspecified role unspecified role bait bait MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 585-1233 Region 585-1233 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human physical association physical association