DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Spring-loaded unraveling of a single SNARE complex by NSF in one round of ATP turnover. Spring-loaded unraveling of a single SNARE complex by NSF in one round of ATP turnover. Science (New York, N.Y.) 2015 imex curation Ryu JK., Min D., Rah SH., Kim SJ., Park Y., Kim H., Hyeon C., Kim HM., Jahn R., Yoon TY. Only protein-protein interactions in vitro In vitro force spectroscopy force spectroscopy intermolecular force predetermined predetermined participant predetermined Ryu JK., Min D., Rah SH., Kim SJ., Park Y., Kim H., Hyeon C., Kim HM., Jahn R., Yoon TY. DIP original experiment: DIP-265394X Science (New York, N.Y.) 2015 IMEx Spring-loaded unraveling of a single SNARE complex by NSF in one round of ATP turnover. Spring-loaded unraveling of a single SNARE complex by NSF in one round of ATP turnover. Spring-loaded unraveling of a single SNARE complex by NSF in one round of ATP turnover. Science (New York, N.Y.) 2015 imex curation Ryu JK., Min D., Rah SH., Kim SJ., Park Y., Kim H., Hyeon C., Kim HM., Jahn R., Yoon TY. Only protein-protein interactions in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Ryu JK., Min D., Rah SH., Kim SJ., Park Y., Kim H., Hyeon C., Kim HM., Jahn R., Yoon TY. DIP original experiment: DIP-265395X Science (New York, N.Y.) 2015 IMEx Spring-loaded unraveling of a single SNARE complex by NSF in one round of ATP turnover. Spring-loaded unraveling of a single SNARE complex by NSF in one round of ATP turnover. Spring-loaded unraveling of a single SNARE complex by NSF in one round of ATP turnover. Science (New York, N.Y.) 2015 imex curation Ryu JK., Min D., Rah SH., Kim SJ., Park Y., Kim H., Hyeon C., Kim HM., Jahn R., Yoon TY. Only protein-protein interactions in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Ryu JK., Min D., Rah SH., Kim SJ., Park Y., Kim H., Hyeon C., Kim HM., Jahn R., Yoon TY. DIP original experiment: DIP-265396X Science (New York, N.Y.) 2015 IMEx Spring-loaded unraveling of a single SNARE complex by NSF in one round of ATP turnover. snaa_bovin Alpha-soluble NSF attachment protein NAPA SNAPA N-ethylmaleimide-sensitive factor attachment protein alpha protein protein bovin Bos taurus (Bovine) Bovine MDNSGKEAEAMALLAEAERKVKNSQSFFSGLFGGSSKIEEACEIYARAANMFKMAKNWSAAGSAFCQAAHVHLQLQSKHDAATCFVDAGNAFKKADPQEAINCLMRAIEIYTDMGRFTIAAKHHISIAEIYETELVDIEKAIAHYEQSADYYKGEESNSSANKCLLKVAGYAAQLEQYQKAIDIYEQVGTNAMDSPLLKYSAKDYFFKAALCHFCIDMLNAKLAVQKYEELFPAFSDSRECKRIKKLLEAHEEQNVDSYTEAVKEYDSISRLDQWLTTMLLRIKKTIQGDEEDLR DIP protein P81125 original sequence version: 82 p60881-2 Synaptosomal-associated protein 25 Snap25 Snap Super protein Synaptosomal-associated 25 kDa protein SNAP-25a protein protein rat Rattus norvegicus (Rat) Rat MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLDRVEEGMNHINQDMKEAEKNLKDLGKCCGLFICPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG DIP protein P60881-2 original sequence version: 54 DIP protein P60881-2 original sequence version: 91 DIP protein P60881-2 original sequence version: 121 DIP protein P60881-2 original sequence version: 126 nsf_crigr Vesicle-fusing ATPase NSF N-ethylmaleimide-sensitive fusion protein Vesicular-fusion protein NSF protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MAGRSMQAARCPTDELSLSNCAVVSEKDYQSGQHVIVRTSPNHKYIFTLRTHPSVVPGSVAFSLPQRKWAGLSIGQEIEVALYSFDKAKQCIGTMTIEIDFLQKKNIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSFNDKLFGLLVKDIEAMDPSILKGEPASGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAKTKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGCKHVKGILLYGPPGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEANIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTVVNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDEKGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQSTAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYASYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKTALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCVVVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAFSTTIHVPNIATGEQLLEALELLGNFKDKERTTIAQQVKGKKVWIGIKKLLMLIEMSLQMDPEYRVRKFLALLREEGASPLDFD DIP protein P18708 original sequence version: 120 DIP protein P18708 original sequence version: 101 DIP protein P18708 original sequence version: 121 F3E0CEB2CF1BD582 vamp2_rat Vesicle-associated membrane protein 2 Vamp2 Syb2 Synaptobrevin-2 protein protein rat Rattus norvegicus (Rat) Rat MSATAATVPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNLKMMIILGVICAIILIIIIVYFST DIP protein P63045 original sequence version: 95 DIP protein P63045 original sequence version: 90 DIP protein P63045 original sequence version: 64 DIP protein P63045 original sequence version: 62 DIP protein P63045 original sequence version: 85 DIP protein P63045 original sequence version: 104 DIP protein P63045 original sequence version: 77 DIP protein P63045 original sequence version: 75 DIP protein P63045 original sequence version: 106 4A0D0D56B5409D0A napa-nsf-5 1 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association vamp2-snap25-2 3 5 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Cy5-label Cy5-label cy5 label cy5 label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role fluorescence donor fluorescence donor Region 1-96 Region 1-96 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Cy3-label Cy3-label cy3 label cy3 label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association vamp2-snap25-3 2 5 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Cy5-label Cy5-label cy5 label cy5 label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role fluorescence donor fluorescence donor Region 1-96 Region 1-96 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Cy3-label Cy3-label cy3 label cy3 label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association