IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Fluorescence resonance energy transfer (FRET) and proximity ligation assays reveal functionally relevant homo- and heteromeric complexes among hyaluronan synthases HAS1, HAS2, and HAS3. Fluorescence resonance energy transfer (FRET) and proximity ligation assays reveal functionally relevant homo- and heteromeric complexes among hyaluronan synthases HAS1, HAS2, and HAS3. The Journal of biological chemistry 2015 imex curation Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI., Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI. PMID: 22159845 for EGFP-HAS3 plasmid - has N-terminal tag. HAS1 GFP = N-terminal, HAS2 = ?-? Text by Fig 7 states "The FRET observed between the N-terminally tagged HAS constructs indicate that the distance between the N termini of two HAS proteins is less than 10 nm." From this I assume all the mcherry mols are N-terminally tagged. tammi@uef.fi 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI., Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI. PMID: 22159845 for EGFP-HAS3 plasmid - has N-terminal tag. HAS1 GFP = N-terminal, HAS2 = ?-? Text by Fig 7 states "The FRET observed between the N-terminally tagged HAS constructs indicate that the distance between the N termini of two HAS proteins is less than 10 nm." From this I assume all the mcherry mols are N-terminally tagged. tammi@uef.fi Accepted 2018-JUN-08 AT 10:34 BST AT 10:34 BST by PPORRAS The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation hope this is clear enough now? Fluorescence resonance energy transfer (FRET) and proximity ligation assays reveal functionally relevant homo- and heteromeric complexes among hyaluronan synthases HAS1, HAS2, and HAS3. Fluorescence resonance energy transfer (FRET) and proximity ligation assays reveal functionally relevant homo- and heteromeric complexes among hyaluronan synthases HAS1, HAS2, and HAS3. The Journal of biological chemistry 2015 imex curation Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI., Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI. PMID: 22159845 for EGFP-HAS3 plasmid - has N-terminal tag. HAS1 GFP = N-terminal, HAS2 = ?-? Text by Fig 7 states "The FRET observed between the N-terminally tagged HAS constructs indicate that the distance between the N termini of two HAS proteins is less than 10 nm." From this I assume all the mcherry mols are N-terminally tagged. tammi@uef.fi 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI., Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI. For the heterodimeric interactions it is not stated in this publication, nor in Refs 34/5, which HAS molecule was transfected with Venus or Cerulean tags. PMID: 22159845 for EGFP-HAS3 plasmid - has N-terminal tag. HAS1 GFP = N-terminal, HAS2 = ?-? Text by Fig 7 states "The FRET observed between the N-terminally tagged HAS constructs indicate that the distance between the N termini of two HAS proteins is less than 10 nm." From this I assume all the mcherry mols are N-terminally tagged. Detection by Flow Cytometry for Venus/YFP tammi@uef.fi Accepted 2018-JUN-08 AT 10:34 BST AT 10:34 BST by PPORRAS The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation hope this is clear enough now? Fluorescence resonance energy transfer (FRET) and proximity ligation assays reveal functionally relevant homo- and heteromeric complexes among hyaluronan synthases HAS1, HAS2, and HAS3. Fluorescence resonance energy transfer (FRET) and proximity ligation assays reveal functionally relevant homo- and heteromeric complexes among hyaluronan synthases HAS1, HAS2, and HAS3. The Journal of biological chemistry 2015 imex curation Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI., Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI. PMID: 22159845 for EGFP-HAS3 plasmid - has N-terminal tag. HAS1 GFP = N-terminal, HAS2 = ?-? Text by Fig 7 states "The FRET observed between the N-terminally tagged HAS constructs indicate that the distance between the N termini of two HAS proteins is less than 10 nm." From this I assume all the mcherry mols are N-terminally tagged. tammi@uef.fi 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI., Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI. For the heterodimeric interactions it is not stated in this publication, nor in Refs 34/5, which HAS molecule was transfected with Venus or Cerulean tags. PMID: 22159845 for EGFP-HAS3 plasmid - has N-terminal tag. HAS1 GFP = N-terminal, HAS2 = ?-? Text by Fig 7 states "The FRET observed between the N-terminally tagged HAS constructs indicate that the distance between the N termini of two HAS proteins is less than 10 nm." From this I assume all the mcherry mols are N-terminally tagged. Detection by Flow Cytometry for Venus/YFP tammi@uef.fi Accepted 2018-JUN-08 AT 10:34 BST AT 10:34 BST by PPORRAS The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation hope this is clear enough now? Fluorescence resonance energy transfer (FRET) and proximity ligation assays reveal functionally relevant homo- and heteromeric complexes among hyaluronan synthases HAS1, HAS2, and HAS3. Fluorescence resonance energy transfer (FRET) and proximity ligation assays reveal functionally relevant homo- and heteromeric complexes among hyaluronan synthases HAS1, HAS2, and HAS3. The Journal of biological chemistry 2015 imex curation Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI., Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI. PMID: 22159845 for EGFP-HAS3 plasmid - has N-terminal tag. HAS1 GFP = N-terminal, HAS2 = ?-? Text by Fig 7 states "The FRET observed between the N-terminally tagged HAS constructs indicate that the distance between the N termini of two HAS proteins is less than 10 nm." From this I assume all the mcherry mols are N-terminally tagged. tammi@uef.fi 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-mesothelium human-mesothelium Human mesothelium pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI., Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI. PMID: 22159845 for EGFP-HAS3 plasmid - has N-terminal tag. HAS1 GFP = N-terminal, HAS2 = ?-? Text by Fig 7 states "The FRET observed between the N-terminally tagged HAS constructs indicate that the distance between the N termini of two HAS proteins is less than 10 nm." From this I assume all the mcherry mols are N-terminally tagged. tammi@uef.fi Accepted 2018-JUN-08 AT 10:34 BST AT 10:34 BST by PPORRAS The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation hope this is clear enough now? Fluorescence resonance energy transfer (FRET) and proximity ligation assays reveal functionally relevant homo- and heteromeric complexes among hyaluronan synthases HAS1, HAS2, and HAS3. Fluorescence resonance energy transfer (FRET) and proximity ligation assays reveal functionally relevant homo- and heteromeric complexes among hyaluronan synthases HAS1, HAS2, and HAS3. The Journal of biological chemistry 2015 imex curation Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI., Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI. PMID: 22159845 for EGFP-HAS3 plasmid - has N-terminal tag. HAS1 GFP = N-terminal, HAS2 = ?-? Text by Fig 7 states "The FRET observed between the N-terminally tagged HAS constructs indicate that the distance between the N termini of two HAS proteins is less than 10 nm." From this I assume all the mcherry mols are N-terminally tagged. tammi@uef.fi 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI., Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI. PMID: 22159845 for EGFP-HAS3 plasmid - has N-terminal tag. HAS1 GFP = N-terminal, HAS2 = ?-? Text by Fig 7 states "The FRET observed between the N-terminally tagged HAS constructs indicate that the distance between the N termini of two HAS proteins is less than 10 nm." From this I assume all the mcherry mols are N-terminally tagged. tammi@uef.fi Accepted 2018-JUN-08 AT 10:34 BST AT 10:34 BST by PPORRAS The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation hope this is clear enough now? Fluorescence resonance energy transfer (FRET) and proximity ligation assays reveal functionally relevant homo- and heteromeric complexes among hyaluronan synthases HAS1, HAS2, and HAS3. Fluorescence resonance energy transfer (FRET) and proximity ligation assays reveal functionally relevant homo- and heteromeric complexes among hyaluronan synthases HAS1, HAS2, and HAS3. The Journal of biological chemistry 2015 imex curation Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI., Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI. PMID: 22159845 for EGFP-HAS3 plasmid - has N-terminal tag. HAS1 GFP = N-terminal, HAS2 = ?-? Text by Fig 7 states "The FRET observed between the N-terminally tagged HAS constructs indicate that the distance between the N termini of two HAS proteins is less than 10 nm." From this I assume all the mcherry mols are N-terminally tagged. tammi@uef.fi 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI., Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI. For the heterodimeric interactions it is not stated in this publication, nor in Refs 34/5, which HAS molecule was transfected with Venus or Cerulean tags. PMID: 22159845 for EGFP-HAS3 plasmid - has N-terminal tag. HAS1 GFP = N-terminal, HAS2 = ?-? Text by Fig 7 states "The FRET observed between the N-terminally tagged HAS constructs indicate that the distance between the N termini of two HAS proteins is less than 10 nm." From this I assume all the mcherry mols are N-terminally tagged. Detection by Flow Cytometry for Venus/YFP tammi@uef.fi Accepted 2018-JUN-08 AT 10:34 BST AT 10:34 BST by PPORRAS The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation hope this is clear enough now? Fluorescence resonance energy transfer (FRET) and proximity ligation assays reveal functionally relevant homo- and heteromeric complexes among hyaluronan synthases HAS1, HAS2, and HAS3. Fluorescence resonance energy transfer (FRET) and proximity ligation assays reveal functionally relevant homo- and heteromeric complexes among hyaluronan synthases HAS1, HAS2, and HAS3. The Journal of biological chemistry 2015 imex curation Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI., Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI. PMID: 22159845 for EGFP-HAS3 plasmid - has N-terminal tag. HAS1 GFP = N-terminal, HAS2 = ?-? Text by Fig 7 states "The FRET observed between the N-terminally tagged HAS constructs indicate that the distance between the N termini of two HAS proteins is less than 10 nm." From this I assume all the mcherry mols are N-terminally tagged. tammi@uef.fi 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI., Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI. PMID: 22159845 for EGFP-HAS3 plasmid - has N-terminal tag. HAS1 GFP = N-terminal, HAS2 = ?-? Text by Fig 7 states "The FRET observed between the N-terminally tagged HAS constructs indicate that the distance between the N termini of two HAS proteins is less than 10 nm." From this I assume all the mcherry mols are N-terminally tagged. Detection by acceptor photobleaching method tammi@uef.fi Accepted 2018-JUN-08 AT 10:34 BST AT 10:34 BST by PPORRAS The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation hope this is clear enough now? Fluorescence resonance energy transfer (FRET) and proximity ligation assays reveal functionally relevant homo- and heteromeric complexes among hyaluronan synthases HAS1, HAS2, and HAS3. Fluorescence resonance energy transfer (FRET) and proximity ligation assays reveal functionally relevant homo- and heteromeric complexes among hyaluronan synthases HAS1, HAS2, and HAS3. The Journal of biological chemistry 2015 imex curation Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI., Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI. PMID: 22159845 for EGFP-HAS3 plasmid - has N-terminal tag. HAS1 GFP = N-terminal, HAS2 = ?-? Text by Fig 7 states "The FRET observed between the N-terminally tagged HAS constructs indicate that the distance between the N termini of two HAS proteins is less than 10 nm." From this I assume all the mcherry mols are N-terminally tagged. tammi@uef.fi 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI., Bart G., Vico NO., Hassinen A., Pujol FM., Deen AJ., Ruusala A., Tammi RH., Squire A., Heldin P., Kellokumpu S., Tammi MI. For the heterodimeric interactions it is not stated in this publication, nor in Refs 34/5, which HAS molecule was transfected with Venus or Cerulean tags. PMID: 22159845 for EGFP-HAS3 plasmid - has N-terminal tag. HAS1 GFP = N-terminal, HAS2 = ?-? Text by Fig 7 states "The FRET observed between the N-terminally tagged HAS constructs indicate that the distance between the N termini of two HAS proteins is less than 10 nm." From this I assume all the mcherry mols are N-terminally tagged. Detection by Flow Cytometry for Venus/YFP tammi@uef.fi Accepted 2018-JUN-08 AT 10:34 BST AT 10:34 BST by PPORRAS The Journal of biological chemistry 2015 Only protein-protein interactions imex curation imex curation hope this is clear enough now? hyas3_human Hyaluronan synthase 3 Hyaluronate synthase 3 Hyaluronic acid synthase 3 HAS3 protein protein human Homo sapiens Human MPVQLTTALRVVGTSLFALAVLGGILAAYVTGYQFIHTEKHYLSFGLYGAILGLHLLIQSLFAFLEHRRMRRAGQALKLPSPRRGSVALCIAAYQEDPDYLRKCLRSAQRISFPDLKVVMVVDGNRQEDAYMLDIFHEVLGGTEQAGFFVWRSNFHEAGEGETEASLQEGMDRVRDVVRASTFSCIMQKWGGKREVMYTAFKALGDSVDYIQVCDSDTVLDPACTIEMLRVLEEDPQVGGVGGDVQILNKYDSWISFLSSVRYWMAFNVERACQSYFGCVQCISGPLGMYRNSLLQQFLEDWYHQKFLGSKCSFGDDRHLTNRVLSLGYRTKYTARSKCLTETPTKYLRWLNQQTRWSKSYFREWLYNSLWFHKHHLWMTYESVVTGFFPFFLIATVIQLFYRGRIWNILLFLLTVQLVGIIKATYACFLRGNAEMIFMSLYSLLYMSSLLPAKIFAIATINKSGWGTSGRKTIVVNFIGLIPVSIWVAVLLGGLAYTAYCQDLFSETELAFLVSGAILYGCYWVALLMLYLAIIARRCGKKPEQYSLAFAEV 82F3B0C932EE9EA3 hyas2_human Hyaluronan synthase 2 Hyaluronate synthase 2 Hyaluronic acid synthase 2 HAS2 protein protein human Homo sapiens Human MHCERFLCILRIIGTTLFGVSLLLGITAAYIVGYQFIQTDNYYFSFGLYGAFLASHLIIQSLFAFLEHRKMKKSLETPIKLNKTVALCIAAYQEDPDYLRKCLQSVKRLTYPGIKVVMVIDGNSEDDLYMMDIFSEVMGRDKSATYIWKNNFHEKGPGETDESHKESSQHVTQLVLSNKSICIMQKWGGKREVMYTAFRALGRSVDYVQVCDSDTMLDPASSVEMVKVLEEDPMVGGVGGDVQILNKYDSWISFLSSVRYWMAFNIERACQSYFGCVQCISGPLGMYRNSLLHEFVEDWYNQEFMGNQCSFGDDRHLTNRVLSLGYATKYTARSKCLTETPIEYLRWLNQQTRWSKSYFREWLYNAMWFHKHHLWMTYEAIITGFFPFFLIATVIQLFYRGKIWNILLFLLTVQLVGLIKSSFASCLRGNIVMVFMSLYSVLYMSSLLPAKMFAIATINKAGWGTSGRKTIVVNFIGLIPVSVWFTILLGGVIFTIYKESKRPFSESKQTVLIVGTLLYACYWVMLLTLYVVLINKCGRRKKGQQYDMVLDV EEEF58D97B131F9D hyas1_human Hyaluronan synthase 1 Hyaluronate synthase 1 Hyaluronic acid synthase 1 HAS1 HAS protein protein human Homo sapiens Human MRQQDAPKPTPAACRCSGLARRVLTIAFALLILGLMTWAYAAGVPLASDRYGLLAFGLYGAFLSAHLVAQSLFAYLEHRRVAAAARGPLDAATARSVALTISAYQEDPAYLRQCLASARALLYPRARLRVLMVVDGNRAEDLYMVDMFREVFADEDPATYVWDGNYHQPWEPAAAGAVGAGAYREVEAEDPGRLAVEALVRTRRCVCVAQRWGGKREVMYTAFKALGDSVDYVQVCDSDTRLDPMALLELVRVLDEDPRVGAVGGDVRILNPLDSWVSFLSSLRYWVAFNVERACQSYFHCVSCISGPLGLYRNNLLQQFLEAWYNQKFLGTHCTFGDDRHLTNRMLSMGYATKYTSRSRCYSETPSSFLRWLSQQTRWSKSYFREWLYNALWWHRHHAWMTYEAVVSGLFPFFVAATVLRLFYAGRPWALLWVLLCVQGVALAKAAFAAWLRGCLRMVLLSLYAPLYMCGLLPAKFLALVTMNQSGWGTSGRRKLAANYVPLLPLALWALLLLGGLVRSVAHEARADWSGPSRAAEAYHLAAGAGAYVGYWVAMLTLYWVGVRRLCRRRTGGYRVQV 2FE3A44B0D5380FF 4565646-1 1 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 1A the existence of HAS1 heteromeric complexes with either HAS2 or HAS3 were also shown we found that EGFP-HAS1 at the expected molecular mass of ∼90 kDa was brought down by mCherry-HAS1 has3-has1-1 1 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 10 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n_terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 1A-2 has2-has1-1 1 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n_terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 1A-3 Image text states that:- "Beads that bind mRFP and mCherry were used to immunoprecipitate (IP) mRFP-HAS3, mCherry-HAS2, and mCherry-HAS1 from COS1 cultures co-transfected with EGFP-HAS1.". However the image labels give two instances of mcherry-HAS2 transfection and NO instance of mcherry-HAS1. It has been assumed that the second instance of mcherry-HAS2 is meant to be mcherry-HAS1. has3-has1-2 1 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 1B has3-has3-1 1 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n_terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 1B-2 has1-has1-4 5 11 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range 11 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization 2-1 has1-has1-5 5 10 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range 11 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization 2-2 has1-has2-1 5 9 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range 11 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization 2-3 has1-has2-2 5 10 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization 2-4 has1-has3-1 5 9 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization 2-5 has2-has2-1 5 9 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization 2-6 has1-has1-6 6 11 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range 11 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 3-1 has1-1 6 10 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 3-2 has2-1 6 9 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 3-3 has1-7 6 11 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 3-4 has1-has2-3 6 11 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 3-5 has1-has3-2 6 10 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 3-6 has1-8 3 11 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range 11 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 3A, top left image; figure 4A, top left image has2-2 3 10 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 3A, top middle image;figure 4B, top middle image has3-1 3 9 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 3A, top right image;figure 4C, top right image has1-has2-4 3 11 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 3B, lower left image; figure 4D, lower left image has2-has3-1 3 10 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 3B, lower right image;figure 4F, lower right image has1-has3-3 3 11 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 3B, lower middle image;figure 4E, middle image-left hand side has2-3 2 10 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 3-2 has3-3 2 9 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 3-3 has1-has2-5 2 11 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 3-4 has2-has3-2 2 10 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 3-6 has1-9 2 11 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range 11 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 3-1 has1-has3-4 2 11 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 3-5 has2-4 8 10 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 3-2 has3-4 8 9 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 3-3 has1-has2-6 8 11 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 3-4 has2-has3-3 8 10 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 3-6 has1-10 8 11 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range 11 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 3-1 has1-has3-5 8 11 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 3-5 has1-11 3 11 competitor competitor competitor unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range 11 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 4A, top left image has2-5 3 10 competitor competitor competitor unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 4B, top middle image has3-5 3 9 competitor competitor competitor unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell c-terminus venus venus fluorescent protein tag venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 4C, top right image has1-has2-7 3 10 competitor competitor competitor unspecified role unspecified role region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 4D, lower left image has1-has3-6 3 11 competitor competitor competitor unspecified role unspecified role region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 4E, lower middle image has1-has1-7 3 9 competitor competitor competitor unspecified role unspecified role region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 4E, right hand side image has2-has3-4 3 10 competitor competitor competitor unspecified role unspecified role region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed living cell living cell c-terminus fluorescent prot tag fluorescent protein tag fluorescent prot tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction Figure 4F, lower right image has1-has2-8 7 11 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain n-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 10 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell n-terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range proximity proximity close-contact colocalization neighbourhood interaction 5A-1/5B, 7A-LHS has1-has2-9 7 11 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell n-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell n-terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range proximity proximity close-contact colocalization neighbourhood interaction 5A-2/5B, has1-has3-7 7 10 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell n-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell n-terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range proximity proximity close-contact colocalization neighbourhood interaction 5A-3/5B has1-has2-10 7 11 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell n-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell n-terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range proximity proximity close-contact colocalization neighbourhood interaction 5B-1 has1-12 7 10 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell n-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 10 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell n-terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range proximity proximity close-contact colocalization neighbourhood interaction 5B-2 has2-6 7 9 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell n-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell n-terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range proximity proximity close-contact colocalization neighbourhood interaction 5B-3, 7A-RHSi ryujsdtujsrxyhuxr-1 4 10 unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level proximity proximity close-contact colocalization neighbourhood interaction figure 6 has2-has2-2 4 11 unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level proximity proximity close-contact colocalization neighbourhood interaction figure 6i weak interaction has2-has2-3 4 9 unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level proximity proximity close-contact colocalization neighbourhood interaction figure 6ii has2-has3-5 7 10 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell n-terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell n-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain proximity proximity close-contact colocalization neighbourhood interaction 7A-RHSii has2-7 7 9 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell n-terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell n-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 286 289 proximity proximity close-contact colocalization neighbourhood interaction 7A-RHSiii has2-has1-2 1 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n-terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminal_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n_terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 7B