DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway. Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway. Science (New York, N.Y.) 2015 imex curation Park SE., Kim JM., Seok OH., Cho H., Wadas B., Kim SY., Varshavsky A., Hwang CS. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Park SE., Kim JM., Seok OH., Cho H., Wadas B., Kim SY., Varshavsky A., Hwang CS. DIP original experiment: DIP-265390X Science (New York, N.Y.) 2015 IMEx Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway. Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway. Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway. Science (New York, N.Y.) 2015 imex curation Park SE., Kim JM., Seok OH., Cho H., Wadas B., Kim SY., Varshavsky A., Hwang CS. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park SE., Kim JM., Seok OH., Cho H., Wadas B., Kim SY., Varshavsky A., Hwang CS. DIP original experiment: DIP-265387X Science (New York, N.Y.) 2015 IMEx Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway. Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway. Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway. Science (New York, N.Y.) 2015 imex curation Park SE., Kim JM., Seok OH., Cho H., Wadas B., Kim SY., Varshavsky A., Hwang CS. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Park SE., Kim JM., Seok OH., Cho H., Wadas B., Kim SY., Varshavsky A., Hwang CS. DIP original experiment: DIP-265389X Science (New York, N.Y.) 2015 IMEx Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway. Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway. Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway. Science (New York, N.Y.) 2015 imex curation Park SE., Kim JM., Seok OH., Cho H., Wadas B., Kim SY., Varshavsky A., Hwang CS. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park SE., Kim JM., Seok OH., Cho H., Wadas B., Kim SY., Varshavsky A., Hwang CS. DIP original experiment: DIP-265388X Science (New York, N.Y.) 2015 IMEx Control of mammalian G protein signaling by N-terminal acetylation and the N-end rule pathway. p41220-1 Regulator of G-protein signaling 2 RGS2 G0S8 GIG31 Cell growth-inhibiting gene 31 protein G0/G1 switch regulatory protein 8 protein protein human Homo sapiens Human MQSAMFLAVQHDCRPMDKSAGSGHKSEEKREKMKRTLLKDWKTRLSYFLQNSSTPGKPKTGKKSKQQAFIKPSPEEAQLWSEAFDELLASKYGLAAFRAFLKSEFCEENIEFWLACEDFKKTKSPQKLSSKARKIYTDFIEKEAPKEINIDFQTKTLIAQNIQEATSGCFTTAQKRVYSLMENNSYPRFLESEFYQDLCKKPQITTEPHAT DIP protein P41220-1 original sequence version: 126 DIP protein P41220-1 original sequence version: 146 ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG marh6_human E3 ubiquitin-protein ligase MARCHF6 MARCHF6 Doa10 homolog KIAA0597 MARCH6 Membrane-associated RING finger protein 6 Membrane-associated RING-CH protein VI Protein TEB-4 RING finger protein 176 RING-type E3 ubiquitin transferase MARCHF6 RNF176 TEB4 protein protein human Homo sapiens Human MDTAEEDICRVCRSEGTPEKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFAFTPIYSPDMPSRLPIQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTENLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPIWLEHAAPPFNAAGHHQNEAPAGGNGAENVAADQPANPPAENAVVGENPDAQDDQAEEEEEDNEEEDDAGVEDAADANNGAQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSLVGLGFEEHVQASHFEGLITTIVGYILLAITLIICHGLATLVKFHRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDATLKDRELSFQSAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVIVFGSIVLLMLWLPIRIIKSVLPNFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEENENSANQQVNNNQHARNNNAIPVVGEGLHAAHQAILQQGGPVGFQPYRRPLNFPLRIFLLIVFMCITLLIASLICLTLPVFAGRWLMSFWTGTAKIHELYTAACGLYVCWLTIRAVTVMVAWMPQGRRVIFQKVKEWSLMIMKTLIVAVLLAGVVPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLHYIVRKLAAPVISVLLLSLCVPYVIASGVVPLLGVTAEMQNLVHRRIYPFLLMVVVLMAILSFQVRQFKRLYEHIKNDKYLVGQRLVNYERKSGKQGSSPPPPQSSQE DIP protein O60337 original sequence version: 110 C7A96FEA45B5CA8D rgs2-march6-1 2 7 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human 5 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human physical association physical association rgs2-march6-2 3 7 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human 5 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Region 1-10 Region 1-10 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain AcM1 AcM1 acmet N-acetyl-L-methionine (2S)-2-acetamido-4-(methylsulfanyl)butanoic acid 2-acetylamino-4-(methylsulfanyl)butanoic acid 2-acetylamino-4-(methylthio)butanoic acid 2-acetylazanyl-4-(methylsulfanyl)butanoic acid MOD_RES N-acetylmethionine N-acetyl-L-methionine N-acetylated L-methionine acetylmethionine methionamine AcMet certain certain sequence position certain certain certain sequence position certain prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm ecolx Escherichia coli physical association physical association march6-rgs2-1 4 7 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human 5 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P41220-1:p.Met1Pro M1P mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain M P This feature has been corrected as a result of our quality control procedures. The original label was 'M1P'. Wed May 02 16:12:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met1pro' Tue Jun 30 16:58:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met1Pro' human Homo sapiens Human physical association physical association rgs2-ub-1 1 6 unspecified role unspecified role prey prey human Homo sapiens Human 5 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human physical association physical association