DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Structure (London, England : 1993) 2015 imex curation Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. DIP original experiment: DIP-264865X Structure (London, England : 1993) 2015 IMEx Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Structure (London, England : 1993) 2015 imex curation Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. Only protein-protein interactions in vitro In vitro thermal shift thermal shift binding thermal shift predetermined predetermined participant predetermined Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. DIP original experiment: DIP-264864X Structure (London, England : 1993) 2015 IMEx Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Structure (London, England : 1993) 2015 imex curation Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. Only protein-protein interactions crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster comigration in sds comigration in sds page comigration in sds anti tag western anti tag western blot anti tag western Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. DIP original experiment: DIP-264871X Structure (London, England : 1993) 2015 IMEx Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Structure (London, England : 1993) 2015 imex curation Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. Only protein-protein interactions ecolx Escherichia coli comigration in sds comigration in sds page comigration in sds weight by comassie molecular weight estimation by coomasie staining weight by comassie Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. DIP original experiment: DIP-264867X Structure (London, England : 1993) 2015 IMEx Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Structure (London, England : 1993) 2015 imex curation Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. Only protein-protein interactions in vitro In vitro solid phase assay solid phase assay predetermined predetermined participant predetermined Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. DIP original experiment: DIP-264868X Structure (London, England : 1993) 2015 IMEx Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Structure (London, England : 1993) 2015 imex curation Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. DIP original experiment: DIP-264866X Structure (London, England : 1993) 2015 IMEx Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Structure (London, England : 1993) 2015 imex curation Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. Only protein-protein interactions in vitro In vitro solid phase assay solid phase assay predetermined predetermined participant predetermined Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. DIP original experiment: DIP-264869X Structure (London, England : 1993) 2015 IMEx Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Structure (London, England : 1993) 2015 imex curation Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. Only protein-protein interactions in vitro In vitro solid phase assay solid phase assay predetermined predetermined participant predetermined Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. DIP original experiment: DIP-264870X Structure (London, England : 1993) 2015 IMEx Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. o60462-6 Neuropilin-2 NRP2 VEGF165R2 Vascular endothelial cell growth factor 165 receptor 2 protein protein human Homo sapiens Human MDMFPLTWVFLALYFSRHQVRGQPDPPCGGRLNSKDAGYITSPGYPQDYPSHQNCEWIVYAPEPNQKIVLNFNPHFEIEKHDCKYDFIEIRDGDSESADLLGKHCGNIAPPTIISSGSMLYIKFTSDYARQGAGFSLRYEIFKTGSEDCSKNFTSPNGTIESPGFPEKYPHNLDCTFTILAKPKMEIILQFLIFDLEHDPLQVGEGDCKYDWLDIWDGIPHVGPLIGKYCGTKTPSELRSSTGILSLTFHTDMAVAKDGFSARYYLVHQEPLENFQCNVPLGMESGRIANEQISASSTYSDGRWTPQQSRLHGDDNGWTPNLDSNKEYLQVDLRFLTMLTAIATQGAISRETQNGYYVKSYKLEVSTNGEDWMVYRHGKNHKVFQANNDATEVVLNKLHAPLLTRFVRIRPQTWHSGIALRLELFGCRVTDAPCSNMLGMLSGLIADSQISASSTQEYLWSPSAARLVSSRSGWFPRIPQAQPGEEWLQVDLGTPKTVKGVIIQGARGGDSITAVEARAFVRKFKVSYSLNGKDWEYIQDPRTQQPKVGCSWRPL DIP protein O60462-6 original sequence version: 142 B67B7C992AB0546F nrp2_human Neuropilin-2 NRP2 VEGF165R2 Vascular endothelial cell growth factor 165 receptor 2 protein protein human Homo sapiens Human MDMFPLTWVFLALYFSRHQVRGQPDPPCGGRLNSKDAGYITSPGYPQDYPSHQNCEWIVYAPEPNQKIVLNFNPHFEIEKHDCKYDFIEIRDGDSESADLLGKHCGNIAPPTIISSGSMLYIKFTSDYARQGAGFSLRYEIFKTGSEDCSKNFTSPNGTIESPGFPEKYPHNLDCTFTILAKPKMEIILQFLIFDLEHDPLQVGEGDCKYDWLDIWDGIPHVGPLIGKYCGTKTPSELRSSTGILSLTFHTDMAVAKDGFSARYYLVHQEPLENFQCNVPLGMESGRIANEQISASSTYSDGRWTPQQSRLHGDDNGWTPNLDSNKEYLQVDLRFLTMLTAIATQGAISRETQNGYYVKSYKLEVSTNGEDWMVYRHGKNHKVFQANNDATEVVLNKLHAPLLTRFVRIRPQTWHSGIALRLELFGCRVTDAPCSNMLGMLSGLIADSQISASSTQEYLWSPSAARLVSSRSGWFPRIPQAQPGEEWLQVDLGTPKTVKGVIIQGARGGDSITAVEARAFVRKFKVSYSLNGKDWEYIQDPRTQQPKLFEGNMHYDTPDIRRFDPIPAQYVRVYPERWSPAGIGMRLEVLGCDWTDSKPTVETLGPTVKSEETTTPYPTEEEATECGENCSFEDDKDLQLPSGFNCNFDFLEEPCGWMYDHAKWLRTTWASSSSPNDRTFPDDRNFLRLQSDSQREGQYARLISPPVHLPRSPVCMEFQYQATGGRGVALQVVREASQESKLLWVIREDQGGEWKHGRIILPSYDMEYQIVFEGVIGKGRSGEIAIDDIRISTDVPLENCMEPISAFAGENFKVDIPEIHEREGYEDEIDDEYEVDWSNSSSATSGSGAPSTDKEKSWLYTLDPILITIIAMSSLGVLLGATCAGLLLYCTCSYSGLSSRSCTTLENYNFELYDGLKHKVKMNHQKCCSEA DIP protein O60462 original sequence version: 133 DIP protein O60462 original sequence version: 142 270CBAE69A0A797C p97953-1 Vascular endothelial growth factor C Vegfc Vascular endothelial growth factor-related protein Vegf-C Flt4 ligand protein protein mouse Mus musculus Mouse MHLLCFLSLACSLLAAALIPSPREAPATVAAFESGLGFSEAEPDGGEVKAFEGKDLEEQLRSVSSVDELMSVLYPDYWKMYKCQLRKGGWQQPTLNTRTGDSVKFAAAHYNTEILKSIDNEWRKTQCMPREVCIDVGKEFGAATNTFFKPPCVSVYRCGGCCNSEGLQCMNTSTGYLSKTLFEITVPLSQGPKPVTISFANHTSCRCMSKLDVYRQVHSIIRRSLPATLPQCQAANKTCPTNYVWNNYMCRCLAQQDFIFYSNVEDDSTNGFHDVCGPNKELDEDTCQCVCKGGLRPSSCGPHKELDRDSCQCVCKNKLFPNSCGANREFDENTCQCVCKRTCPRNQPLNPGKCACECTENTQKCFLKGKKFHHQTCSCYRRPCANRLKHCDPGLSFSEEVCRCVPSYWKRPHLN DIP protein P97953-1 original sequence version: 125 vgfr3_human Vascular endothelial growth factor receptor 3 Tyrosine-protein kinase receptor FLT4 FLT4 Fms-like tyrosine kinase 4 VEGFR3 protein protein human Homo sapiens Human MQRGAALCLRLWLCLGLLDGLVSGYSMTPPTLNITEESHVIDTGDSLSISCRGQHPLEWAWPGAQEAPATGDKDSEDTGVVRDCEGTDARPYCKVLLLHEVHANDTGSYVCYYKYIKARIEGTTAASSYVFVRDFEQPFINKPDTLLVNRKDAMWVPCLVSIPGLNVTLRSQSSVLWPDGQEVVWDDRRGMLVSTPLLHDALYLQCETTWGDQDFLSNPFLVHITGNELYDIQLLPRKSLELLVGEKLVLNCTVWAEFNSGVTFDWDYPGKQAERGKWVPERRSQQTHTELSSILTIHNVSQHDLGSYVCKANNGIQRFRESTEVIVHENPFISVEWLKGPILEATAGDELVKLPVKLAAYPPPEFQWYKDGKALSGRHSPHALVLKEVTEASTGTYTLALWNSAAGLRRNISLELVVNVPPQIHEKEASSPSIYSRHSRQALTCTAYGVPLPLSIQWHWRPWTPCKMFAQRSLRRRQQQDLMPQCRDWRAVTTQDAVNPIESLDTWTEFVEGKNKTVSKLVIQNANVSAMYKCVVSNKVGQDERLIYFYVTTIPDGFTIESKPSEELLEGQPVLLSCQADSYKYEHLRWYRLNLSTLHDAHGNPLLLDCKNVHLFATPLAASLEEVAPGARHATLSLSIPRVAPEHEGHYVCEVQDRRSHDKHCHKKYLSVQALEAPRLTQNLTDLLVNVSDSLEMQCLVAGAHAPSIVWYKDERLLEEKSGVDLADSNQKLSIQRVREEDAGRYLCSVCNAKGCVNSSASVAVEGSEDKGSMEIVILVGTGVIAVFFWVLLLLIFCNMRRPAHADIKTGYLSIIMDPGEVPLEEQCEYLSYDASQWEFPRERLHLGRVLGYGAFGKVVEASAFGIHKGSSCDTVAVKMLKEGATASEHRALMSELKILIHIGNHLNVVNLLGACTKPQGPLMVIVEFCKYGNLSNFLRAKRDAFSPCAEKSPEQRGRFRAMVELARLDRRRPGSSDRVLFARFSKTEGGARRASPDQEAEDLWLSPLTMEDLVCYSFQVARGMEFLASRKCIHRDLAARNILLSESDVVKICDFGLARDIYKDPDYVRKGSARLPLKWMAPESIFDKVYTTQSDVWSFGVLLWEIFSLGASPYPGVQINEEFCQRLRDGTRMRAPELATPAIRRIMLNCWSGDPKARPAFSELVEILGDLLQGRGLQEEEEVCMAPRSSQSSEEGSFSQVSTMALHIAQADAEDSPPSLQRHSLAARYYNWVSFPGCLARGAETRGSSRMKTFEEFPMTPTTYKGSVDNQTDSGMVLASEEFEQIESRHRQESGFSCKGPGQNVAVTRAHPDSQGRRRRPERGARGGQVFYNSEYGELSEPSEEDHCSPSARVTFFTDNSY DIP protein P35916 original sequence version: 173 B1473AAAC95E7E93 nrp2-vegfc-1 2 11 unspecified role unspecified role unspecified role unspecified role Region 215-223 Region 215-223 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 10 unspecified role unspecified role unspecified role unspecified role Region 279-595 Region 279-595 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction nrp2-2 3 9 unspecified role unspecified role unspecified role unspecified role Region 275-555 Region 275-555 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Hgh-tag Hgh-tag fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster disulfide bond disulfide bond disulfide bridge SS-bond nrp2-3 4 9 unspecified role unspecified role unspecified role unspecified role Region 275-555 Region 275-555 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli disulfide bond disulfide bond disulfide bridge SS-bond nrp2-vegfc-2 7 11 unspecified role unspecified role prey prey Region 108-223 Region 108-223 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 10 unspecified role unspecified role bait bait Region 279-595 Region 279-595 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O60462:p.Thr319Arg T319R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain T R This feature has been corrected as a result of our quality control procedures. The original label was 'T319R'. Wed May 02 17:40:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr319arg' Tue Jun 30 19:26:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr319Arg' ecolx Escherichia coli direct interaction direct interaction flt4-vegfc-1 8 12 unspecified role unspecified role bait bait Region 21-776 Region 21-776 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 11 unspecified role unspecified role prey prey Region 108-223 Region 108-223 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster direct interaction direct interaction nrp2-vegfc-3 5 11 unspecified role unspecified role prey prey Region 108-223 Region 108-223 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P97953-1:p.Arg223Glu R223E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R E This feature has been corrected as a result of our quality control procedures. The original label was 'R223E'. Wed May 02 14:36:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg223glu' Tue Jun 30 17:15:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg223Glu' crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster 10 unspecified role unspecified role bait bait Region279-595 Region279-595 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction nrp2-4 1 9 unspecified role unspecified role unspecified role unspecified role Region 275-555 Region 275-555 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction nrp2-5 6 9 unspecified role unspecified role unspecified role unspecified role Region 275-555 Region 275-555 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction