DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu PROTEIN TARGETING TO STARCH is required for localising GRANULE-BOUND STARCH SYNTHASE to starch granules and for normal amylose synthesis in Arabidopsis. PROTEIN TARGETING TO STARCH is required for localising GRANULE-BOUND STARCH SYNTHASE to starch granules and for normal amylose synthesis in Arabidopsis. PLoS biology 2015 imex curation Seung D., Soyk S., Coiro M., Maier BA., Eicke S., Zeeman SC. Only protein-protein interactions nicbe Nicotiana benthamiana anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Seung D., Soyk S., Coiro M., Maier BA., Eicke S., Zeeman SC. DIP original experiment: DIP-264897X PLoS biology 2015 IMEx PROTEIN TARGETING TO STARCH is required for localising GRANULE-BOUND STARCH SYNTHASE to starch granules and for normal amylose synthesis in Arabidopsis. PROTEIN TARGETING TO STARCH is required for localising GRANULE-BOUND STARCH SYNTHASE to starch granules and for normal amylose synthesis in Arabidopsis. PROTEIN TARGETING TO STARCH is required for localising GRANULE-BOUND STARCH SYNTHASE to starch granules and for normal amylose synthesis in Arabidopsis. PLoS biology 2015 imex curation Seung D., Soyk S., Coiro M., Maier BA., Eicke S., Zeeman SC. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Seung D., Soyk S., Coiro M., Maier BA., Eicke S., Zeeman SC. DIP original experiment: DIP-264896X PLoS biology 2015 IMEx PROTEIN TARGETING TO STARCH is required for localising GRANULE-BOUND STARCH SYNTHASE to starch granules and for normal amylose synthesis in Arabidopsis. ptst_arath Protein PTST, chloroplastic PTST1 MKM21.80 At5g39790 PTST PROTEIN TARGETING TO STARCH protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGCVPRIEFGCSSQSLTLSWNLRAWNLCRLNTISHFQKLPYPLVASTRKHYKNSLLLKRFLVGVGTEESSLSEDLLDESLSRPLTSDELKSLLIDTERSKLVKKLSEANQQNRFLKRQLKTQEHEITNIKTELALMELEVQALVKLAEEIANLGIPQGSRKISGKYIQSHLLSRLDAVQKKMKEQIKGVEAAQSKEVHVFWIGMAESVQVMGSFDGWSQREDLSPEYSALFTKFSTTLFLRPGRYEMKFLVDGEWQISPEFPTSGEGLMENNVLVVE DIP protein Q94AX2 original sequence version: 54 E5D7C697F6B8464F ssg1_arath Granule-bound starch synthase 1, chloroplastic/amyloplastic GBSS1 GBS1 GBSS Granule-bound starch synthase I WAXY F9L11.8 At1g32900 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MATVTASSNFVSRTSLFNNHGASSCSDVAQITLKGQSLTHCGLRSFNMVDNLQRRSQAKPVSAKSSKRSSKVKTAGKIVCEKGMSVIFIGAEVGPWSKTGGLGDVLGGLPPALAARGHRVMTICPRYDQYKDAWDTCVVVQIKVGDKVENVRFFHCYKRGVDRVFVDHPIFLAKVVGKTGSKIYGPITGVDYNDNQLRFSLLCQAALEAPQVLNLNSSKYFSGPYGEDVVFVANDWHTALLPCYLKSMYQSRGVYMNAKVVFCIHNIAYQGRFAFDDYSLLNLPISFKSSFDFMDGYEKPVKGRKINWMKAAILEAHRVLTVSPYYAQELISGVDRGVELHKYLRMKTVSGIINGMDVQEWNPSTDKYIDIKYDITTVTDAKPLIKEALQAAVGLPVDRDVPVIGFIGRLEEQKGSDILVEAISKFMGLNVQMVILGTGKKKMEAQILELEEKFPGKAVGVAKFNVPLAHMITAGADFIIVPSRFEPCGLIQLHAMRYGTVPIVASTGGLVDTVKDGYTGFHIGRFNVKCEVVDPDDVIATAKAVTRAVAVYGTSAMQEMVKNCMDQDFSWKGPARLWEKVLLSLNVAGSEAGTEGEEIAPLAKENVATP DIP protein Q9MAQ0 original sequence version: 99 CF17F25BE12220DF gbss1-ptst-1 2 3 unspecified role unspecified role prey prey GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 45-277 Region 45-277 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 4 unspecified role unspecified role bait bait Q9MAQ0:p.Lys441Glu K441E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K E This feature has been corrected as a result of our quality control procedures. The original label was 'K441E'. Wed May 02 14:38:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys441glu' Tue Jun 30 18:08:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys441Glu' Q9MAQ0:p.Glu449Lys E449K mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E K This feature has been corrected as a result of our quality control procedures. The original label was 'E449K'. Wed May 02 17:24:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu449lys' Tue Jun 30 19:06:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu449Lys' Q9MAQ0:p.Glu452Lys E452K mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E K This feature has been corrected as a result of our quality control procedures. The original label was 'E452K'. Wed May 02 17:16:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu452lys' Tue Jun 30 18:14:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu452Lys' Q9MAQ0:p.[Lys441Glu;Glu452Lys] K441E/E452K mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E K certain certain sequence position certain certain certain sequence position certain K E This feature has been corrected as a result of our quality control procedures. The original label was 'K441E/E452K'. Wed May 02 15:50:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys441glu,glu452lys' Tue Jun 30 17:46:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys441Glu;Glu452Lys]' His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Region 80-610 Region 80-610 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ptst-gbss1-1 1 3 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range nicbe Nicotiana benthamiana 4 unspecified role unspecified role prey prey Q9MAQ0:p.Glu452Lys E452K mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E K This feature has been corrected as a result of our quality control procedures. The original label was 'E452K'. Wed May 02 15:51:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu452lys' Tue Jun 30 18:37:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu452Lys' Q9MAQ0:p.[Lys441Glu;Glu452Lys] K441E/E452K mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E K certain certain sequence position certain certain certain sequence position certain K E This feature has been corrected as a result of our quality control procedures. The original label was 'K441E/E452K'. Wed May 02 15:47:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys441glu,glu452lys' Tue Jun 30 16:08:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys441Glu;Glu452Lys]' HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range nicbe Nicotiana benthamiana physical association physical association