DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu A conserved activation cluster is required for allosteric communication in HtrA-family proteases. A conserved activation cluster is required for allosteric communication in HtrA-family proteases. Structure (London, England : 1993) 2015 imex curation de Regt AK., Kim S., Sohn J., Grant RA., Baker TA., Sauer RT. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined de Regt AK., Kim S., Sohn J., Grant RA., Baker TA., Sauer RT. DIP original experiment: DIP-264826X Structure (London, England : 1993) 2015 IMEx A conserved activation cluster is required for allosteric communication in HtrA-family proteases. A conserved activation cluster is required for allosteric communication in HtrA-family proteases. A conserved activation cluster is required for allosteric communication in HtrA-family proteases. Structure (London, England : 1993) 2015 imex curation de Regt AK., Kim S., Sohn J., Grant RA., Baker TA., Sauer RT. Only protein-protein interactions in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined de Regt AK., Kim S., Sohn J., Grant RA., Baker TA., Sauer RT. DIP original experiment: DIP-264827X Structure (London, England : 1993) 2015 IMEx A conserved activation cluster is required for allosteric communication in HtrA-family proteases. rsea_ecoli Anti-sigma-E factor RseA yfiJ rseA mclA b2572 JW2556 Regulator of SigE Sigma-E anti-sigma factor RseA Sigma-E factor negative regulatory protein protein protein ecoli Escherichia coli (strain K12) MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMRGDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLTQMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANNGQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ DIP protein P0AFX7 original sequence version: 71 DIP protein P0AFX7 original sequence version: 40 DIP protein P0AFX7 original sequence version: 39 DIP protein P0AFX7 original sequence version: 76 63BD12131C611C32 degs_ecoli Serine endoprotease DegS degS hhoB htrH b3235 JW3204 Site-1 protease DegS Site-1-type intramembrane protease protein protein ecoli Escherichia coli (strain K12) MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETPASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDADQIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGNPYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTLSFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGIDQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVVVMRDDKQLTLQVTIQEYPATN DIP protein P0AEE3 original sequence version: 45 DIP protein P0AEE3 original sequence version: 43 DIP protein P0AEE3 original sequence version: 85 D091B4D65E8FE1CC dnrdgnvyyf OMP peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) DNRDGNVYYF degs-omp-1 1 5 unspecified role unspecified role unspecified role unspecified role Fluorescein-label Fluorescein-label fluorescein fluorescein label fluorescein n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal in vitro In vitro 4 unspecified role unspecified role unspecified role unspecified role Region 27-355 Region 27-355 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction degs-rsea-7 2 3 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 121-216 Region 121-216 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 4 enzyme enzyme unspecified role unspecified role Region 27-355 Region 27-355 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0AEE3:p.His198Pro H198P mutation increasing rate mutation increasing interaction rate mutation increasing rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain H P This feature has been corrected as a result of our quality control procedures. The original label was 'H198P'. Wed May 02 20:28:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his198pro' Tue Jun 30 19:38:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His198Pro' P0AEE3:p.Pro161Ala P161A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain P A This feature has been corrected as a result of our quality control procedures. The original label was 'P161A'. Wed May 02 16:15:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro161ala' Tue Jun 30 19:18:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro161Ala' P0AEE3:p.Tyr162Ala Y162A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y162A'. Wed May 02 19:41:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr162ala' Tue Jun 30 17:17:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr162Ala' P0AEE3:p.Leu164Ala L164A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L164A'. Wed May 02 20:32:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu164ala' Tue Jun 30 18:12:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu164Ala' P0AEE3:p.Thr167Val T167V mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain T V This feature has been corrected as a result of our quality control procedures. The original label was 'T167V'. Wed May 02 16:59:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr167val' Tue Jun 30 19:10:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr167Val' P0AEE3:p.Thr169Ala T169A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A This feature has been corrected as a result of our quality control procedures. The original label was 'T169A'. Wed May 02 16:31:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr169ala' Tue Jun 30 18:55:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr169Ala' P0AEE3:p.Arg178Ala R178A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R178A'. Wed May 02 15:55:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg178ala' Tue Jun 30 17:20:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg178Ala' P0AEE3:p.Ile179Ala I179A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I179A'. Wed May 02 18:09:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile179ala' Tue Jun 30 18:23:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile179Ala' P0AEE3:p.Gln187Ala Q187A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Q A This feature has been corrected as a result of our quality control procedures. The original label was 'Q187A'. Wed May 02 18:51:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln187ala' Tue Jun 30 16:32:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln187Ala' P0AEE3:p.Gln191Ala Q191A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Q A This feature has been corrected as a result of our quality control procedures. The original label was 'Q191A'. Wed May 02 18:43:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln191ala' Tue Jun 30 17:32:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln191Ala' P0AEE3:p.Asn197Ala N197A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain N A This feature has been corrected as a result of our quality control procedures. The original label was 'N197A'. Wed May 02 15:46:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn197ala' Tue Jun 30 18:39:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn197Ala' P0AEE3:p.Phe220Ala F220A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F220A'. Wed May 02 17:17:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe220ala' Tue Jun 30 18:09:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe220Ala' P0AEE3:p.Asp221Ala D221A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D221A'. Wed May 02 20:25:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp221ala' Tue Jun 30 15:39:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp221Ala' P0AEE3:p.Pro229Ala P229A mutation increasing rate mutation increasing interaction rate mutation increasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain P A This feature has been corrected as a result of our quality control procedures. The original label was 'P229A'. Wed May 02 16:03:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro229ala' Tue Jun 30 18:58:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro229Ala' P0AEE3:p.Glu230Ala E230A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E230A'. Wed May 02 15:16:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu230ala' Tue Jun 30 19:16:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu230Ala' P0AEE3:p.Ile232Ala I232A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I232A'. Wed May 02 18:46:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile232ala' Tue Jun 30 20:11:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile232Ala' P0AEE3:p.Phe234Ala F234A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F234A'. Wed May 02 19:42:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe234ala' Tue Jun 30 16:14:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe234Ala' ecolx Escherichia coli protein cleavage protein cleavage