DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure (London, England : 1993) 2015 imex curation Solomonson M., Setiaputra D., Makepeace KA., Lameignere E., Petrotchenko EV., Conrady DG., Bergeron JR., Vuckovic M., DiMaio F., Borchers CH., Yip CK., Strynadka NC. Only protein-protein interactions mycsm Mycobacterium smegmatis dhfr reconstruction dihydrofolate reductase reconstruction dhfr reconstruction predetermined predetermined participant predetermined Solomonson M., Setiaputra D., Makepeace KA., Lameignere E., Petrotchenko EV., Conrady DG., Bergeron JR., Vuckovic M., DiMaio F., Borchers CH., Yip CK., Strynadka NC. DIP original experiment: DIP-264819X Structure (London, England : 1993) 2015 IMEx Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure (London, England : 1993) 2015 imex curation Solomonson M., Setiaputra D., Makepeace KA., Lameignere E., Petrotchenko EV., Conrady DG., Bergeron JR., Vuckovic M., DiMaio F., Borchers CH., Yip CK., Strynadka NC. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Solomonson M., Setiaputra D., Makepeace KA., Lameignere E., Petrotchenko EV., Conrady DG., Bergeron JR., Vuckovic M., DiMaio F., Borchers CH., Yip CK., Strynadka NC. DIP original experiment: DIP-264817X Structure (London, England : 1993) 2015 IMEx Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure (London, England : 1993) 2015 imex curation Solomonson M., Setiaputra D., Makepeace KA., Lameignere E., Petrotchenko EV., Conrady DG., Bergeron JR., Vuckovic M., DiMaio F., Borchers CH., Yip CK., Strynadka NC. Only protein-protein interactions ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Solomonson M., Setiaputra D., Makepeace KA., Lameignere E., Petrotchenko EV., Conrady DG., Bergeron JR., Vuckovic M., DiMaio F., Borchers CH., Yip CK., Strynadka NC. DIP original experiment: DIP-264813X Structure (London, England : 1993) 2015 IMEx Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure (London, England : 1993) 2015 imex curation Solomonson M., Setiaputra D., Makepeace KA., Lameignere E., Petrotchenko EV., Conrady DG., Bergeron JR., Vuckovic M., DiMaio F., Borchers CH., Yip CK., Strynadka NC. Only protein-protein interactions ecolx Escherichia coli crosslink cross-linking study crosslink predetermined predetermined participant predetermined Solomonson M., Setiaputra D., Makepeace KA., Lameignere E., Petrotchenko EV., Conrady DG., Bergeron JR., Vuckovic M., DiMaio F., Borchers CH., Yip CK., Strynadka NC. DIP original experiment: DIP-264815X Structure (London, England : 1993) 2015 IMEx Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure (London, England : 1993) 2015 imex curation Solomonson M., Setiaputra D., Makepeace KA., Lameignere E., Petrotchenko EV., Conrady DG., Bergeron JR., Vuckovic M., DiMaio F., Borchers CH., Yip CK., Strynadka NC. Only protein-protein interactions ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Solomonson M., Setiaputra D., Makepeace KA., Lameignere E., Petrotchenko EV., Conrady DG., Bergeron JR., Vuckovic M., DiMaio F., Borchers CH., Yip CK., Strynadka NC. DIP original experiment: DIP-264812X Structure (London, England : 1993) 2015 IMEx Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure (London, England : 1993) 2015 imex curation Solomonson M., Setiaputra D., Makepeace KA., Lameignere E., Petrotchenko EV., Conrady DG., Bergeron JR., Vuckovic M., DiMaio F., Borchers CH., Yip CK., Strynadka NC. Only protein-protein interactions ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Solomonson M., Setiaputra D., Makepeace KA., Lameignere E., Petrotchenko EV., Conrady DG., Bergeron JR., Vuckovic M., DiMaio F., Borchers CH., Yip CK., Strynadka NC. DIP original experiment: DIP-264814X Structure (London, England : 1993) 2015 IMEx Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure (London, England : 1993) 2015 imex curation Solomonson M., Setiaputra D., Makepeace KA., Lameignere E., Petrotchenko EV., Conrady DG., Bergeron JR., Vuckovic M., DiMaio F., Borchers CH., Yip CK., Strynadka NC. Only protein-protein interactions mycsm Mycobacterium smegmatis dhfr reconstruction dihydrofolate reductase reconstruction dhfr reconstruction predetermined predetermined participant predetermined Solomonson M., Setiaputra D., Makepeace KA., Lameignere E., Petrotchenko EV., Conrady DG., Bergeron JR., Vuckovic M., DiMaio F., Borchers CH., Yip CK., Strynadka NC. DIP original experiment: DIP-264820X Structure (London, England : 1993) 2015 IMEx Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure (London, England : 1993) 2015 imex curation Solomonson M., Setiaputra D., Makepeace KA., Lameignere E., Petrotchenko EV., Conrady DG., Bergeron JR., Vuckovic M., DiMaio F., Borchers CH., Yip CK., Strynadka NC. Only protein-protein interactions ecolx Escherichia coli comigration in sds comigration in sds page comigration in sds weight by staining molecular weight estimation by staining weight by staining Solomonson M., Setiaputra D., Makepeace KA., Lameignere E., Petrotchenko EV., Conrady DG., Bergeron JR., Vuckovic M., DiMaio F., Borchers CH., Yip CK., Strynadka NC. DIP original experiment: DIP-264811X Structure (London, England : 1993) 2015 IMEx Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure (London, England : 1993) 2015 imex curation Solomonson M., Setiaputra D., Makepeace KA., Lameignere E., Petrotchenko EV., Conrady DG., Bergeron JR., Vuckovic M., DiMaio F., Borchers CH., Yip CK., Strynadka NC. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Solomonson M., Setiaputra D., Makepeace KA., Lameignere E., Petrotchenko EV., Conrady DG., Bergeron JR., Vuckovic M., DiMaio F., Borchers CH., Yip CK., Strynadka NC. DIP original experiment: DIP-264818X Structure (London, England : 1993) 2015 IMEx Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure (London, England : 1993) 2015 imex curation Solomonson M., Setiaputra D., Makepeace KA., Lameignere E., Petrotchenko EV., Conrady DG., Bergeron JR., Vuckovic M., DiMaio F., Borchers CH., Yip CK., Strynadka NC. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Solomonson M., Setiaputra D., Makepeace KA., Lameignere E., Petrotchenko EV., Conrady DG., Bergeron JR., Vuckovic M., DiMaio F., Borchers CH., Yip CK., Strynadka NC. DIP original experiment: DIP-264810X Structure (London, England : 1993) 2015 IMEx Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. Structure (London, England : 1993) 2015 imex curation Solomonson M., Setiaputra D., Makepeace KA., Lameignere E., Petrotchenko EV., Conrady DG., Bergeron JR., Vuckovic M., DiMaio F., Borchers CH., Yip CK., Strynadka NC. Only protein-protein interactions ecolx Escherichia coli crosslink cross-linking study crosslink predetermined predetermined participant predetermined Solomonson M., Setiaputra D., Makepeace KA., Lameignere E., Petrotchenko EV., Conrady DG., Bergeron JR., Vuckovic M., DiMaio F., Borchers CH., Yip CK., Strynadka NC. DIP original experiment: DIP-264816X Structure (London, England : 1993) 2015 IMEx Structure of EspB from the ESX-1 type VII secretion system and insights into its export mechanism. espb_myctu ESX-1 secretion-associated protein EspB espB mtb48 MTV027.16c Rv3881c Antigen MTB48 protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MTQSQTVTVDQQEILNRANEVEAPMADPPTDVPITPCELTAAKNAAQQLVLSADNMREYLAAGAKERQRLATSLRNAAKAYGEVDEEAATALDNDGEGTVQAESAGAVGGDSSAELTDTPRVATAGEPNFMDLKEAARKLETGDQGASLAHFADGWNTFNLTLQGDVKRFRGFDNWEGDAATACEASLDQQRQWILHMAKLSAAMAKQAQYVAQLHVWARREHPTYEDIVGLERLYAENPSARDQILPVYAEYQQRSEKVLTEYNNKAALEPVNPPKPPPAIKIDPPPPPQEQGLIPGFLMPPSDGSGVTPGTGMPAAPMVPPTGSPGGGLPADTAAQLTSAGREAAALSGDVAVKAASLGGGGGGGVPSAPLGSAIGGAESVRPAGAGDIAGLGQGRAGGGAALGGGGMGMPMGAAHQGQGGAKSKGSQQEDEALYTEDRAWTEAVIGNRRRQDSKESK DIP protein P9WJD9 original sequence version: 9 esxa_myctu 6 kDa early secretory antigenic target esxA esaT6 Rv3875 MTV027.10 ESAT-6 protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MTEQQWNFAGIEAAASAIQGNVTSIHSLLDEGKQSLTKLAAAWGGSGSEAYQGVQQKWDATATELNNALQNLARTISEAGQAMASTEGNVTGMFA DIP protein P9WNK7 original sequence version: 8 DIP protein P9WNK7 original sequence version: 10 79BD529E3D88F519 a0qnk4_mycs2 MSMEG_0076 protein protein mycs2 Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) MSEELQYELPGLERKAHECESTRPEGPGDATKPDELATTASVYSKLMASAAKLKATFAAGDREGERIAAAIRAAAGAYQKIEEQKAAELSRQMNGSDAPPPAAEAVVPDMSGIPGPLAIPSMEYPSAAAAADEMDWEAAARIIHSGDTQALSMKYFRDQWRDYQSTLEGHGRHFANPAEGWAGAAAETCAEAQRRLSTWWADMGAECGRLAQEATTFVDAHDKLVANHPTLENVREFEETEWASEWDRQNAWAMLQEQSEDALEAYANGSQIQEIRPGKPPSIGGLPAVNDGDVQASPTSAPGGPGGPGSGTPGGGGAGGGGGTPEMPELPSTDPSMSPMSANSAGEEQSSGSPSSGGSPSGSPSGGSPSGGGAPSGAGMPDGGLPSDLPGGPDIPGLDDPSLKPASAGGGGGGGVGGGGGGMPAAPLGPAVGADSVSPSPSSTRGGGVGVPGGPGGGAGGMMGGGMGGMGAGHGQGQGKEKKRDPKLAPDEDLYTEDRAHTEGVIGHRARREKDSGKQQ DIP protein A0QNK4 original sequence version: 45 esxb_myctu ESAT-6-like protein EsxB esxB cfp10 lhp mtsA10 Rv3874 MTV027.09 10 kDa culture filtrate antigen CFP-10 Secreted antigenic protein MTSA-10 protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MAEMKTDAATLAQEAGNFERISGDLKTQIDQVESTAGSLQGQWRGAAGTAAQAAVVRFQEAANKQKQELDEISTNIRQAGVQYSRADEEQQQALSSQMGF DIP protein P9WNK5 original sequence version: 8 DIP protein P9WNK5 original sequence version: 10 285F4FC96F55D194 ecc1b_mycse ESX-1 secretion system protein EccCb1 eccCb1 0062 D806_0062 D806_000590 protein protein mycse Mycobacterium smegmatis (strain MKD8) MSTEAEPRVLREVVLSQLATGESRAYKMWLPPLTDPTPVNELVERDYQRRPLRFGLGIMDEPRRHRQEVWGVDVSAAGGNIAVGGAPQTGKSTFLQTLVVSAAATHTPRQVQFYCVDLGGGGLMYLEDLPHVGGVATRAEPDRVNRVVAEVKAVLRAREQVFKQYRVGSIASYREMRDDPSNPASQDPFGDVFLVIDGWPAFVAEFPDLEPAVQDIAGQGLAYGVHVIITTPRWTELKSRVRDYLGTKIEFRLGDVNETQIDRITREIPANRPGRAVSLEKHHLMMGVPRLDGVHSADNIVEAISSAVQQIAGRHTDQAPQVRVLPERIYLHQLDPNPPGPDSDYRTRWQVPLGVRESDLTVAYNQMHLTPHLLIFGAPKSGKTRIAHAVAQAICKRNSPQQVRFMLADYRSGLLDAVPQSHLLDAGAINRNSATLEEAIKALAVNLKKRLPPPDLTTAQLRARSWWSGPDVVLLVDDWHMVTAAAGMVSPMAPLGPLLPAAADIGLHVIVTCQMSMAHRATMDKFVGAAYGAGSPTLFLSGEKNDFPSRDIIVKKRPPGQAFLVGPDGKEVIQAAYVDPPEEEVFSPPSEGS DIP protein L8FPJ2 original sequence version: 12 espb-1 6 12 unspecified role unspecified role unspecified role unspecified role Region 1-348 Region 1-348 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction a0qnk4_mycs2-1 2 14 unspecified role unspecified role unspecified role unspecified role Region 3-288 Region 3-288 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction espb-2 10 12 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction a0qnk4_mycs2-ecccb1-1 7 14 unspecified role unspecified role unspecified role unspecified role Region 70-100 Region 70-100 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mycsm Mycobacterium smegmatis 16 unspecified role unspecified role unspecified role unspecified role Region 341-593 Region 341-593 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mycsm Mycobacterium smegmatis physical association physical association espb-3 8 12 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction a0qnk4_mycs2-2 9 14 unspecified role unspecified role unspecified role unspecified role Region 8-296 Region 8-296 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction espb-4 4 12 unspecified role unspecified role unspecified role unspecified role Region 1-348 Region 1-348 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain CL-K259 CL-K259 xlnk-res-res crosslinked residues XLNK-Res-Res modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction espb-5 5 12 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction espb-6 3 12 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction espb-7 11 12 unspecified role unspecified role unspecified role unspecified role Region 1-348 Region 1-348 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain CL-K259/267 CL-K259/267 xlnk-res-res crosslinked residues XLNK-Res-Res modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction esxa-esxb-4 1 15 unspecified role unspecified role unspecified role unspecified role mycsm Mycobacterium smegmatis 13 unspecified role unspecified role unspecified role unspecified role mycsm Mycobacterium smegmatis physical association physical association