IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Induction and activation of latent transforming growth factor-β1 are carried out by two distinct domains of pregnancy-specific glycoprotein 1 (PSG1). Induction and activation of latent transforming growth factor-β1 are carried out by two distinct domains of pregnancy-specific glycoprotein 1 (PSG1). The Journal of biological chemistry 2015 imex curation Ballesteros A., Mentink-Kane MM., Warren J., Kaplan GG., Dveksler GS., Ballesteros A., Mentink-Kane MM., Warren J., Kaplan GG., Dveksler GS. The domain boundaries for PSG1 are not given in this publication nor the stated reference (no 15). Primers for the B2 domain in methods do not blat, blast or translate to any known sequence. Figure 6C gives domain boundaries for N terminus of the N-terminal domain, which corresponds to those given in UniProt entry P11464. However PMID: 27389696 (2016 publication by same group) gives domain boundaries for PSG1 B2 domain as 331-414, rather than UniProt 335-410. gabriela.dveksler@usuhs.edu 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Ballesteros A., Mentink-Kane MM., Warren J., Kaplan GG., Dveksler GS., Ballesteros A., Mentink-Kane MM., Warren J., Kaplan GG., Dveksler GS. The domain boundaries for PSG1 are not given in this publication nor the stated reference (no 15). Primers for the B2 domain in methods do not blat, blast or translate to any known sequence. Figure 6C gives domain boundaries for N terminus of the N-terminal domain, which corresponds to those given in UniProt entry P11464. However PMID: 27389696 (2016 publication by same group) gives domain boundaries for PSG1 B2 domain as 331-414, rather than UniProt 335-410. gabriela.dveksler@usuhs.edu Accepted 2017-AUG-17 AT 13:49 BST AT 13:49 BST by ORCHARD The Journal of biological chemistry 2015 imex curation Induction and activation of latent transforming growth factor-β1 are carried out by two distinct domains of pregnancy-specific glycoprotein 1 (PSG1). Induction and activation of latent transforming growth factor-β1 are carried out by two distinct domains of pregnancy-specific glycoprotein 1 (PSG1). The Journal of biological chemistry 2015 imex curation Ballesteros A., Mentink-Kane MM., Warren J., Kaplan GG., Dveksler GS., Ballesteros A., Mentink-Kane MM., Warren J., Kaplan GG., Dveksler GS. The domain boundaries for PSG1 are not given in this publication nor the stated reference (no 15). Primers for the B2 domain in methods do not blat, blast or translate to any known sequence. Figure 6C gives domain boundaries for N terminus of the N-terminal domain, which corresponds to those given in UniProt entry P11464. However PMID: 27389696 (2016 publication by same group) gives domain boundaries for PSG1 B2 domain as 331-414, rather than UniProt 335-410. gabriela.dveksler@usuhs.edu 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Ballesteros A., Mentink-Kane MM., Warren J., Kaplan GG., Dveksler GS., Ballesteros A., Mentink-Kane MM., Warren J., Kaplan GG., Dveksler GS. The domain boundaries for PSG1 are not given in this publication nor the stated reference (no 15). Primers for the B2 domain in methods do not blat, blast or translate to any known sequence. Figure 6C gives domain boundaries for N terminus of the N-terminal domain, which corresponds to those given in UniProt entry P11464. However PMID: 27389696 (2016 publication by same group) gives domain boundaries for PSG1 B2 domain as 331-414, rather than UniProt 335-410. gabriela.dveksler@usuhs.edu Accepted 2017-AUG-17 AT 13:49 BST AT 13:49 BST by ORCHARD The Journal of biological chemistry 2015 imex curation psg1_human Pregnancy-specific beta-1-glycoprotein 1 Pregnancy-specific beta-1 glycoprotein C/D Fetal liver non-specific cross-reactive antigen 1/2 PSG95 CD66 antigen-like family member F PSG1 B1G1 PSGGA PSBG1 protein protein human Homo sapiens Human MGTLSAPPCTQRIKWKGLLLTASLLNFWNLPTTAQVTIEAEPTKVSEGKDVLLLVHNLPQNLTGYIWYKGQMRDLYHYITSYVVDGEIIIYGPAYSGRETAYSNASLLIQNVTREDAGSYTLHIIKGDDGTRGVTGRFTFTLHLETPKPSISSSNLNPRETMEAVSLTCDPETPDASYLWWMNGQSLPMTHSLKLSETNRTLFLLGVTKYTAGPYECEIRNPVSASRSDPVTLNLLPKLPKPYITINNLNPRENKDVLNFTCEPKSENYTYIWWLNGQSLPVSPRVKRPIENRILILPSVTRNETGPYQCEIRDRYGGIRSDPVTLNVLYGPDLPRIYPSFTYYRSGEVLYLSCSADSNPPAQYSWTINEKFQLPGQKLFIRHITTKHSGLYVCSVRNSATGKESSKSMTVEVSDWTVP 5E9855162F0C796D p01137-pro_0000033762 Latency-associated peptide TGFB1 TGFB protein protein human Homo sapiens Human LSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRR 30 278 12D7E59F5DF7639A tgfb1_human Transforming growth factor beta-1 proprotein TGFB1 TGFB protein protein human Homo sapiens Human MPPSGLRLLLLLLPLLWLLVLTPGRPAAGLSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFLLLMATPLERAQHLQSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS DIP protein P01137 original sequence version: 149 DIP protein P01137 original sequence version: 185 DIP protein P01137 original sequence version: 160 75391614250288FE psg1-fc-1-slc-1 2 3 unspecified role unspecified role prey prey signal+B2_region sufficient to bind sufficient binding region sufficient to bind range range range range range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 5 unspecified role unspecified role bait bait slc sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. direct interaction direct interaction 2A,3A tgfb1-psg1-1 2 3 unspecified role unspecified role prey prey signal+B2_region sufficient to bind sufficient binding region sufficient to bind range range range range range range range range c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 4 unspecified role unspecified role bait bait spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. direct interaction direct interaction 2B, 3B, 5A tgfb1-psg1-2 2 3 unspecified role unspecified role prey prey n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 5 unspecified role unspecified role bait bait slc sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. direct interaction direct interaction 2Ai tgfb1-psg1-3 2 3 unspecified role unspecified role prey prey c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 4 unspecified role unspecified role bait bait spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. direct interaction direct interaction 2Bi tgfb1-psg1-4 1 3 unspecified role unspecified role prey prey B2_region sufficient to bind sufficient binding region sufficient to bind range range range range n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus s tag s tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role bait bait slc sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. direct interaction direct interaction 4 A/C