DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure (London, England : 1993) 2015 imex curation Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. DIP original experiment: DIP-264722X Structure (London, England : 1993) 2015 IMEx Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure (London, England : 1993) 2015 imex curation Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. DIP original experiment: DIP-264724X Structure (London, England : 1993) 2015 IMEx Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure (London, England : 1993) 2015 imex curation Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. DIP original experiment: DIP-264723X Structure (London, England : 1993) 2015 IMEx Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure (London, England : 1993) 2015 imex curation Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. DIP original experiment: DIP-264718X Structure (London, England : 1993) 2015 IMEx Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure (London, England : 1993) 2015 imex curation Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. DIP original experiment: DIP-264719X Structure (London, England : 1993) 2015 IMEx Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure (London, England : 1993) 2015 imex curation Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. DIP original experiment: DIP-264721X Structure (London, England : 1993) 2015 IMEx Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure (London, England : 1993) 2015 imex curation Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. DIP original experiment: DIP-264716X Structure (London, England : 1993) 2015 IMEx Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure (London, England : 1993) 2015 imex curation Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. DIP original experiment: DIP-264720X Structure (London, England : 1993) 2015 IMEx Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure (London, England : 1993) 2015 imex curation Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. DIP original experiment: DIP-264717X Structure (London, England : 1993) 2015 IMEx Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. Structure (London, England : 1993) 2015 imex curation Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Glatt S., Zabel R., Vonkova I., Kumar A., Netz DJ., Pierik AJ., Rybin V., Lill R., Gavin AC., Balbach J., Breunig KD., Müller CW. DIP original experiment: DIP-264715X Structure (London, England : 1993) 2015 IMEx Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2. elp3_yeast Elongator complex protein 3 ELP3 Gamma-toxin target 3 HPA1 TOT3 tRNA uridine(34) acetyltransferase YPL086C protein protein yeast Saccharomyces cerevisiae Baker's yeast MARHGKGPKTNKKKLAPEKERFIQCCADITLELTDSLTSGTTREINLNGLITKYSKKYKLKQQPRLTDIINSIPDQYKKYLLPKLKAKPVRTASGIAVVAVMCKPHRCPHIAYTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYDPYEQARGRVEQLKQLGHSIDKVEYVLMGGTFMSLPKEYREDFIVKLHNALSGFNGNDIDEAILYSQQSLTKCVGITIETRPDYCTQTHLDDMLKYGCTRLEIGVQSLYEDVARDTNRGHTVRSVCETFAVSKDAGYKVVSHMMPDLPNVGMERDIEQFKEYFENPDFRTDGLKIYPTLVIRGTGLYELWKTGRYKSYSANALVDLVARILALVPPWTRIYRVQRDIPMPLVTSGVDNGNLRELALARMKDLGTTCRDVRTREVGIQEVHHKVQPDQVELIRRDYYANGGWETFLSYEDPKKDILIGLLRLRKASKKYTYRKEFTSQRTSIVRELHVYGSVVPLHSRDPRKFQHQGFGTLLMEEAERIAKEEHGSEKISVISGVGVRNYYGKLGYELDGPYMSKRI 64EF8B42D3C3102E 64EF8B42D3C3102E dph1_yeast 2-(3-amino-3-carboxypropyl)histidine synthase subunit 1 DPH1 Diphthamide biosynthesis protein 1 Diphtheria toxin resistance protein 1 S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase 1 YIL103W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSGSTESKKQPRRRFIGRKSGNSNNDKLTTVAENGNEIIHKQKSRIALGRSVNHVPEDILNDKELNEAIKLLPSNYNFEIHKTVWNIRKYNAKRIALQMPEGLLIYSLIISDILEQFCGVETLVMGDVSYGACCIDDFTARALDCDFIVHYAHSCLVPIDVTKIKVLYVFVTINIQEDHIIKTLQKNFPKGSRIATFGTIQFNPAVHSVRDKLLNDEEHMLYIIPPQIKPLSRGEVLGCTSERLDKEQYDAMVFIGDGRFHLESAMIHNPEIPAFKYDPYNRKFTREGYDQKQLVEVRAEAIEVARKGKVFGLILGALGRQGNLNTVKNLEKNLIAAGKTVVKIILSEVFPQKLAMFDQIDVFVQVACPRLSIDWGYAFNKPLLTPYEASVLLKKDVMFSEKYYPMDYYEAKGYGRGETPKHAIE 22E93852E03C6722 22E93852E03C6722 dph3_yeast Diphthamide biosynthesis protein 3 DPH3 Kluyveromyces lactis toxin-insensitive protein 11 YBL071W-A KTI11 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTYDEIEIEDMTFEPENQMFTYPCPCGDRFQIYLDDMFEGEKVAVCPSCSLMIDVVFDKEDLAEYYEEAGIHPPEPIAAAA 8B27A307831B0ECE 8B27A307831B0ECE ats1_yeast Protein KTI13 Alpha-tubulin suppressor 1 ATS1 KTI13 Kluyveromyces lactis toxin-insensitive protein 13 FUN28 YAL006 YAL020C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSCVYAFGSNGQRQLGLGHDEDMDTPQRSVPGDDGAIVRKIACGGNHSVMLTNDGNLVGCGDNRRGELDSAQALRQVHDWRPVEVPAPVVDVACGWDTTVIVDADGRVWQRGGGCYEFTQQHVPLNSNDERIAVYGCFQNFVVVQGTRVYGWGSNTKCQLQEPKSRSLKEPVLVYDTGSVAVDYVAMGKDFMVIVDEGGRIVHASGRLPTGFELKQQQKRHNLVVLCMWTSIHLWNARLNTVESFGRGTHSQLFPQERLDFPIVGVATGSEHGILTTANQEGKSHCYNVYCWGWGEHGNCGPQKGSQPGLQLVGQYSGKPRVFGGCATTWIVL 3C6F4BE180479096 3C6F4BE180479096 kti11-kti13-1 10 14 unspecified role unspecified role prey prey ecolx Escherichia coli 13 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q3E840:p.Tyr4Ala Y4A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y4A'. Wed May 02 19:53:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr4ala' Tue Jun 30 18:05:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr4Ala' Q3E840:p.Asp5Ala D5A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D5A'. Wed May 02 17:39:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp5ala' Tue Jun 30 19:16:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp5Ala' Q3E840:p.Glu8Ala E8A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E8A'. Wed May 02 18:42:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu8ala' Tue Jun 30 16:38:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu8Ala' Region 1-7 Region 1-7 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction kti11-dph1-4 2 12 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 13 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q3E840:p.Cys50Ser C50S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain C S This feature has been corrected as a result of our quality control procedures. The original label was 'C50S'. Wed May 02 20:21:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys50ser' Tue Jun 30 18:52:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys50Ser' Q3E840:p.Cys25Ser C25S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain C S This feature has been corrected as a result of our quality control procedures. The original label was 'C25S'. Wed May 02 17:48:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys25ser' Tue Jun 30 19:37:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys25Ser' Q3E840:p.Cys27Ser C27S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain C S This feature has been corrected as a result of our quality control procedures. The original label was 'C27S'. Wed May 02 19:34:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys27ser' Tue Jun 30 17:59:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys27Ser' Q3E840:p.Cys47Ser C47S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain C S This feature has been corrected as a result of our quality control procedures. The original label was 'C47S'. Wed May 02 18:04:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys47ser' Tue Jun 30 19:27:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys47Ser' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association kti11-kti13-2 4 14 unspecified role unspecified role unspecified role unspecified role P31386:p.Trp229Ala W229A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W229A'. Wed May 02 14:31:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp229ala' Tue Jun 30 19:28:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp229Ala' P31386:p.Glu296Ala E296A mutation increasing strength mutation increasing interaction strength mutation increasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E296A'. Wed May 02 18:19:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu296ala' Tue Jun 30 17:39:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu296Ala' P31386:p.His297Ala H297A mutation increasing strength mutation increasing interaction strength mutation increasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain H A This feature has been corrected as a result of our quality control procedures. The original label was 'H297A'. Wed May 02 15:29:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his297ala' Tue Jun 30 19:56:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His297Ala' ecolx Escherichia coli 13 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction kti11-kti13-3 8 14 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 13 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction kti11-kti13-4 6 13 unspecified role unspecified role bait bait Q3E840:p.Tyr4Ala Y4A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y4A'. Wed May 02 17:36:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr4ala' Tue Jun 30 16:46:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr4Ala' Region 1-7 Region 1-7 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q3E840:p.Tyr4_Asp5delinsAlaAla Y4A/D4A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain YD AA Wed May 02 19:56:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr_asp4-5ala_ala' Tue Jun 30 20:01:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr4_Asp5delinsAlaAla' HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q3E840:p.[Ile7Ala;Ile9Ala] I7A/I9A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain I A certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I7A/I9A'. Wed May 02 17:50:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile7ala,ile9ala' Tue Jun 30 18:40:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile7Ala;Ile9Ala]' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 14 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association kti11-kti13-5 1 13 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 14 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P31386:p.Trp229Cys W229C mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain W C This feature has been corrected as a result of our quality control procedures. The original label was 'W229C'. Wed May 02 16:50:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp229cys' Tue Jun 30 17:07:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp229Cys' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association kti11-kti13-6 9 14 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 13 unspecified role unspecified role unspecified role unspecified role Q3E840:p.Tyr4Ala Y4A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y4A'. Wed May 02 19:41:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr4ala' Tue Jun 30 16:57:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr4Ala' Q3E840:p.Asp5Ala D5A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D5A'. Wed May 02 16:43:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp5ala' Tue Jun 30 18:12:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp5Ala' Q3E840:p.Tyr4_Asp5delinsAlaAla Y4A/D4A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain YD AA Wed May 02 18:51:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr_asp4-5ala_ala' Tue Jun 30 19:03:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr4_Asp5delinsAlaAla' Q3E840:p.Glu8Ala E8A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E8A'. Wed May 02 17:28:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu8ala' Tue Jun 30 16:43:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu8Ala' Q3E840:p.[Glu6Ala;Glu8Ala] E6A/E8A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E6A/E8A'. Wed May 02 18:06:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu6ala,glu8ala' Tue Jun 30 16:08:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu6Ala;Glu8Ala]' ecolx Escherichia coli direct interaction direct interaction kti11-kti13-7 5 14 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 13 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction kti11-kti13-8 7 14 unspecified role unspecified role prey prey P31386:p.Trp229Cys W229C mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain W C This feature has been corrected as a result of our quality control procedures. The original label was 'W229C'. Wed May 02 15:54:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp229cys' Tue Jun 30 16:02:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp229Cys' P31386:p.Trp229Ala W229A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W229A'. Wed May 02 16:40:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp229ala' Tue Jun 30 17:23:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp229Ala' P31386:p.Trp294Ala W294A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W294A'. Wed May 02 19:32:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp294ala' Tue Jun 30 18:31:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp294Ala' ecolx Escherichia coli 13 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction kti11-elp3-1 3 11 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 13 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q3E840:p.Cys50Ser C50S mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain C S This feature has been corrected as a result of our quality control procedures. The original label was 'C50S'. Wed May 02 15:16:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys50ser' Tue Jun 30 15:58:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys50Ser' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association