DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Electrostatic interactions between the Bni1p Formin FH2 domain and actin influence actin filament nucleation. Electrostatic interactions between the Bni1p Formin FH2 domain and actin influence actin filament nucleation. Structure (London, England : 1993) 2015 imex curation Baker JL., Courtemanche N., Parton DL., McCullagh M., Pollard TD., Voth GA. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Baker JL., Courtemanche N., Parton DL., McCullagh M., Pollard TD., Voth GA. DIP original experiment: DIP-264684X Structure (London, England : 1993) 2015 IMEx Electrostatic interactions between the Bni1p Formin FH2 domain and actin influence actin filament nucleation. Electrostatic interactions between the Bni1p Formin FH2 domain and actin influence actin filament nucleation. Electrostatic interactions between the Bni1p Formin FH2 domain and actin influence actin filament nucleation. Structure (London, England : 1993) 2015 imex curation Baker JL., Courtemanche N., Parton DL., McCullagh M., Pollard TD., Voth GA. Only protein-protein interactions in vitro In vitro fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Baker JL., Courtemanche N., Parton DL., McCullagh M., Pollard TD., Voth GA. DIP original experiment: DIP-264685X Structure (London, England : 1993) 2015 IMEx Electrostatic interactions between the Bni1p Formin FH2 domain and actin influence actin filament nucleation. acts_chick Actin, alpha skeletal muscle ACTA1 ACTA Alpha-actin-1 protein protein chick Gallus gallus (Chicken) Chicken MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF DIP protein P68139 original sequence version: 86 DF2A3A046346A179 bni1_yeast Protein BNI1 PPF3 SHE5 BNI1 YNL271C N0646 Synthetic lethal 39 Sensitive to high expression protein 5 Pointed projection formation protein 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLKNSGSKHSNSKESHSNSSSGIFQNLKRLANSNATNSNTGSPTYASQQQHSPVGNEVSTSPASSSSFRKLNAPSRSTSTEARPLNKKSTLNTQNLSQYMNGKLSGDVPVSSQHARSHSMQSKYSYSKRNSSQASNKLTRQHTGQSHSASSLLSQGSLTNLSKFTTPDGKIYLEMPSDPYEVEVLFEDIMYKRNIFQSLSEDKQEALMGYSIEKKWLIVKQDLQNELKKMRANTTSSSTASRTSMASDHHPILTANSSLSSPKSVLMTSASSPTSTVYSNSLNHSTTLSSVGTSTSKGKKLVSGSLKKQPSLNNIYRGGAENNTSASTLPGDRTNRPPIHYVQRILADKLTSDEMKDLWVTLRTEQLDWVDAFIDHQGHIAMANVLMNSIYKTAPRENLTKELLEKENSFFKCFRVLSMLSQGLYEFSTHRLMTDTVAEGLFSTKLATRKMATEIFVCMLEKKNKSRFEAVLTSLDKKFRIGQNLHMIQNFKKMPQYFSHLTLESHLKIIQAWLFAVEQTLDGRGKMGSLVGASDEFKNGGGENAILEYCQWTMVFINHLCSCSDNINQRMLLRTKLENCGILRIMNKIKLLDYDKVIDQIELYDNNKLDDFNVKLEANNKAFNVDLHDPLSLLKNLWDICKGTENEKLLVSLVQHLFLSSSKLIEENQNSSKLTKQLKLMDSLVTNVSVASTSDEETNMNMAIQRLYDAMQTDEVARRAILESRALTKKLEEIQAERDSLSEKLSKAEHGLVGQLEDELHERDRILAKNQRVMQQLEAELEELKKKHLLEKHQQEVELRKMLTILNSRPEESFNKNEGTRGMNSSLNSSEKANIQKVLQDGLSRAKKDYKDDSKKFGMTLQPNKRLKMLRMQMENIENEARQLEMTNFAEFEKDRLEPPIHIKKPKVKKMKNKDRKPLVKPQEADVNKLNDLRRALAEIQMESNDISKFNVEERVNELFNEKKSLALKRLKELETKYKGFGIDFNVDEIMDSPKKNTGDVETEEDANYASLDPKTYQKKLDEINRITDQLLDIQTQTEHEIQVEEDGESDLSSSSSDDESEEIYQDASPTQELRSEHSELSSGSGPGSFLDALSQKYGTGQNVTASAAFGENNNGSGIGPLHSKVEKTFMNRLRKSTVSSAPYLEELTQKVNKVEPYEQNEDEGLDKKSLPENSTASAASAFDKAEKDMRQHVENGKQGRVVNHEEDKTADFSAVSKLNNTDGAEDLSTQSSVLSSQPPPPPPPPPPVPAKLFGESLEKEKKSEDDTVKQETTGDSPAPPPPPPPPPPPPMALFGKPKGETPPPPPLPSVLSSSTDGVIPPAPPMMPASQIKSAVTSPLLPQSPSLFEKYPRPHKKLKQLHWEKLDCTDNSIWGTGKAEKFADDLYEKGVLADLEKAFAAREIKSLASKRKEDLQKITFLSRDISQQFGINLHMYSSLSVADLVKKILNCDRDFLQTPSVVEFLSKSEIIEVSVNLARNYAPYSTDWEGVRNLEDAKPPEKDPNDLQRADQIYLQLMVNLESYWGSRMRALTVVTSYEREYNELLAKLRKVDKAVSALQESDNLRNVFNVILAVGNFMNDTSKQAQGFKLSTLQRLTFIKDTTNSMTFLNYVEKIVRLNYPSFNDFLSELEPVLDVVKVSIEQLVNDCKDFSQSIVNVERSVEIGNLSDSSKFHPLDKVLIKTLPVLPEARKKGDLLEDEVKLTIMEFESLMHTYGEDSGDKFAKISFFKKFADFINEYKKAQAQNLAAEEEERLYIKHKKIVEEQQKRAQEKEKQKENSNSPSSEGNEEDEAEDRRAVMDKLLEQLKNAGPAKSDPSSARKRALVRKKYLSEKDNAPQLLNDLDTEEGSILYSPEAMDPTADTVIHAESPTPLATRGVMNTSEDLPSPSKTSALEDQEEISDRARMLLKELRGSDTPVKQNSILDEHLEKLRARKERSIGEASTGNRLSFK 0C5A9D5280A5858F 0C5A9D5280A5858F acta1-bni1p-1 1 3 unspecified role unspecified role unspecified role unspecified role Pyrene-label Pyrene-label pyrene label pyrene label certain certain sequence position certain certain certain sequence position certain chick Gallus gallus (Chicken) Chicken 4 unspecified role unspecified role unspecified role unspecified role Region 1312-1766 Region 1312-1766 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P41832:p.[Arg1423Asn;Lys1467Leu] R1423N/K1467L mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K L certain certain sequence position certain certain certain sequence position certain R N This feature has been corrected as a result of our quality control procedures. The original label was 'R1423N/K1467L'. Wed May 02 17:31:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1423asn,lys1467leu' Tue Jun 30 19:11:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg1423Asn;Lys1467Leu]' P41832:p.Arg1423Asn R1423N mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R N This feature has been corrected as a result of our quality control procedures. The original label was 'R1423N'. Wed May 02 16:00:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1423asn' Tue Jun 30 19:00:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1423Asn' P41832:p.Lys1467Leu K1467L mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K L This feature has been corrected as a result of our quality control procedures. The original label was 'K1467L'. Wed May 02 16:18:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys1467leu' Tue Jun 30 16:48:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys1467Leu' ecolx Escherichia coli direct interaction direct interaction acta1-bni1p-2 2 3 unspecified role unspecified role unspecified role unspecified role Oregon-label Oregon-label oregon green label oregon green label certain certain sequence position certain certain certain sequence position certain chick Gallus gallus (Chicken) Chicken 4 unspecified role unspecified role unspecified role unspecified role Region 1312-1766 Region 1312-1766 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P41832:p.[Arg1423Asn;Lys1467Leu] R1423N/K1467L mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K L certain certain sequence position certain certain certain sequence position certain R N This feature has been corrected as a result of our quality control procedures. The original label was 'R1423N/K1467L'. Wed May 02 16:48:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1423asn,lys1467leu' Tue Jun 30 18:54:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg1423Asn;Lys1467Leu]' P41832:p.Arg1423Asn R1423N mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R N This feature has been corrected as a result of our quality control procedures. The original label was 'R1423N'. Wed May 02 18:00:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1423asn' Tue Jun 30 16:37:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1423Asn' P41832:p.Lys1467Leu K1467L mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K L This feature has been corrected as a result of our quality control procedures. The original label was 'K1467L'. Wed May 02 14:45:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys1467leu' Tue Jun 30 19:03:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys1467Leu' ecolx Escherichia coli direct interaction direct interaction