DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu MapZ marks the division sites and positions FtsZ rings in Streptococcus pneumoniae. MapZ marks the division sites and positions FtsZ rings in Streptococcus pneumoniae. Nature 2014 imex curation Fleurie A., Lesterlin C., Manuse S., Zhao C., Cluzel C., Lavergne JP., Franz-Wachtel M., Macek B., Combet C., Kuru E., VanNieuwenhze MS., Brun YV., Sherratt D., Grangeasse C. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Fleurie A., Lesterlin C., Manuse S., Zhao C., Cluzel C., Lavergne JP., Franz-Wachtel M., Macek B., Combet C., Kuru E., VanNieuwenhze MS., Brun YV., Sherratt D., Grangeasse C. DIP original experiment: DIP-264454X Nature 2014 IMEx MapZ marks the division sites and positions FtsZ rings in Streptococcus pneumoniae. MapZ marks the division sites and positions FtsZ rings in Streptococcus pneumoniae. MapZ marks the division sites and positions FtsZ rings in Streptococcus pneumoniae. Nature 2014 imex curation Fleurie A., Lesterlin C., Manuse S., Zhao C., Cluzel C., Lavergne JP., Franz-Wachtel M., Macek B., Combet C., Kuru E., VanNieuwenhze MS., Brun YV., Sherratt D., Grangeasse C. Only protein-protein interactions stree Streptococcus pneumoniae anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Fleurie A., Lesterlin C., Manuse S., Zhao C., Cluzel C., Lavergne JP., Franz-Wachtel M., Macek B., Combet C., Kuru E., VanNieuwenhze MS., Brun YV., Sherratt D., Grangeasse C. DIP original experiment: DIP-264452X Nature 2014 IMEx MapZ marks the division sites and positions FtsZ rings in Streptococcus pneumoniae. MapZ marks the division sites and positions FtsZ rings in Streptococcus pneumoniae. MapZ marks the division sites and positions FtsZ rings in Streptococcus pneumoniae. Nature 2014 imex curation Fleurie A., Lesterlin C., Manuse S., Zhao C., Cluzel C., Lavergne JP., Franz-Wachtel M., Macek B., Combet C., Kuru E., VanNieuwenhze MS., Brun YV., Sherratt D., Grangeasse C. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Fleurie A., Lesterlin C., Manuse S., Zhao C., Cluzel C., Lavergne JP., Franz-Wachtel M., Macek B., Combet C., Kuru E., VanNieuwenhze MS., Brun YV., Sherratt D., Grangeasse C. DIP original experiment: DIP-264453X Nature 2014 IMEx MapZ marks the division sites and positions FtsZ rings in Streptococcus pneumoniae. stkp_strr6 Serine/threonine-protein kinase StkP stkP pkn2 spr1577 Eukaryotic-type Ser/Thr protein kinase protein protein strr6 Streptococcus pneumoniae (strain ATCC BAA-255 / R6) MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVLRTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYIKEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVAFAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAVTIALQHFQNPLPSVIAENSSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYNRRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQAPKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANFEIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSDVIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKATTIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVSKPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVEGMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP DIP protein Q8DNS0 original sequence version: 94 q8dnv9_strr6 Cell division protein FtsZ ftsZ spr1510 protein protein strr6 Streptococcus pneumoniae (strain ATCC BAA-255 / R6) MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAANTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFITAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVDTLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTVMANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEAEEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATNYRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSVVSPVERFEAPISQDEDELDTPPFFKNR DIP protein Q8DNV9 original sequence version: 85 mapz_strr6 Mid-cell-anchored protein Z mapZ locZ spr0334 protein protein strr6 Streptococcus pneumoniae (strain ATCC BAA-255 / R6) MSKKRRNRHKKEAQEPQFDFDEAKELTVGQAIRKNEEVEAGVLPEDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEVPSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGFILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTNSNKTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIVDGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSSQASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAWDFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGYFVGNGAGHADDLDY DIP protein Q8DR55 original sequence version: 53 mapz-ftsz-1 2 5 unspecified role unspecified role prey prey stree Streptococcus pneumoniae 6 unspecified role unspecified role bait bait Region 1-158 Region 1-158 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range stree Streptococcus pneumoniae physical association physical association mapz-stkp-1 3 6 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli 4 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation ftsz-mapz-1 1 5 unspecified role unspecified role bait bait ecolx Escherichia coli 6 unspecified role unspecified role prey prey Region 1-41 Region 1-41 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction