DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Crystal structure of the human, FIC-domain containing protein HYPE and implications for its functions. Crystal structure of the human, FIC-domain containing protein HYPE and implications for its functions. Structure (London, England : 1993) 2014 imex curation Bunney TD., Cole AR., Broncel M., Esposito D., Tate EW., Katan M. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Bunney TD., Cole AR., Broncel M., Esposito D., Tate EW., Katan M. DIP original experiment: DIP-264331X Structure (London, England : 1993) 2014 IMEx Crystal structure of the human, FIC-domain containing protein HYPE and implications for its functions. Crystal structure of the human, FIC-domain containing protein HYPE and implications for its functions. Crystal structure of the human, FIC-domain containing protein HYPE and implications for its functions. Structure (London, England : 1993) 2014 imex curation Bunney TD., Cole AR., Broncel M., Esposito D., Tate EW., Katan M. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Bunney TD., Cole AR., Broncel M., Esposito D., Tate EW., Katan M. DIP original experiment: DIP-264329X Structure (London, England : 1993) 2014 IMEx Crystal structure of the human, FIC-domain containing protein HYPE and implications for its functions. Crystal structure of the human, FIC-domain containing protein HYPE and implications for its functions. Crystal structure of the human, FIC-domain containing protein HYPE and implications for its functions. Structure (London, England : 1993) 2014 imex curation Bunney TD., Cole AR., Broncel M., Esposito D., Tate EW., Katan M. Only protein-protein interactions ecolx Escherichia coli saxs x ray scattering saxs predetermined predetermined participant predetermined Bunney TD., Cole AR., Broncel M., Esposito D., Tate EW., Katan M. DIP original experiment: DIP-264330X Structure (London, England : 1993) 2014 IMEx Crystal structure of the human, FIC-domain containing protein HYPE and implications for its functions. Crystal structure of the human, FIC-domain containing protein HYPE and implications for its functions. Crystal structure of the human, FIC-domain containing protein HYPE and implications for its functions. Structure (London, England : 1993) 2014 imex curation Bunney TD., Cole AR., Broncel M., Esposito D., Tate EW., Katan M. Only protein-protein interactions in vitro In vitro ampylation assay ampylation assay ampylation assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Bunney TD., Cole AR., Broncel M., Esposito D., Tate EW., Katan M. DIP original experiment: DIP-264332X Structure (London, England : 1993) 2014 IMEx Crystal structure of the human, FIC-domain containing protein HYPE and implications for its functions. ficd_human Protein adenylyltransferase FICD FICD HIP13 HYPE AMPylator FICD FIC domain-containing protein Huntingtin yeast partner E Huntingtin-interacting protein 13 Huntingtin-interacting protein E UNQ3041/PRO9857 De-AMPylase FICD protein protein human Homo sapiens Human MMLIPMASVMAVTEPKWVSVWSRFLWVTLLSMVLGSLLALLLPLGAVEEQCLAVLKGLYLLRSKPDRAQHAATKCTSPSTELSITSRGATLLVAKTKASPAGKLEARAALNQALEMKRQGKREKAQKLFMHALKMDPDFVDALTEFGIFSEEDKDIIQADYLYTRALTISPYHEKALVNRDRTLPLVEEIDQRYFSIIDSKVKKVMSIPKGNSALRRVMEETYYHHIYHTVAIEGNTLTLSEIRHILETRYAVPGKSLEEQNEVIGMHAAMKYINTTLVSRIGSVTISDVLEIHRRVLGYVDPVEAGRFRTTQVLVGHHIPPHPQDVEKQMQEFVQWLNSEEAMNLHPVEFAALAHYKLVYIHPFIDGNGRTSRLLMNLILMQAGYPPITIRKEQRSDYYHVLEAANEGDVRPFIRFIAKCTETTLDTLLFATTEYSVALPEAQPNHSGFKETLPVKP DIP protein Q9BVA6 original sequence version: 107 DIP protein Q9BVA6 original sequence version: 88 8393EAA46D61C48E ficd-1 2 5 unspecified role unspecified role unspecified role unspecified role Region 103-445 Region 103-445 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9BVA6:p.Leu258Asp L258D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L D This feature has been corrected as a result of our quality control procedures. The original label was 'L258D'. Wed May 02 15:20:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu258asp' Tue Jun 30 15:51:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu258Asp' ecolx Escherichia coli direct interaction direct interaction ficd-2 4 5 putative self putative self unspecified role unspecified role Region 103-445 Region 103-445 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9BVA6:p.Glu234Gly E234G mutation increasing rate mutation increasing interaction rate mutation increasing rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E G This feature has been corrected as a result of our quality control procedures. The original label was 'E234G'. Wed May 02 16:04:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu234gly' Tue Jun 30 18:29:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu234Gly' ecolx Escherichia coli ampylation ampylation reaction ampylation true ficd-3 3 5 unspecified role unspecified role unspecified role unspecified role Region 103-445 Region 103-445 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9BVA6:p.Leu258Asp L258D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L D This feature has been corrected as a result of our quality control procedures. The original label was 'L258D'. Wed May 02 19:10:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu258asp' Tue Jun 30 16:32:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu258Asp' ecolx Escherichia coli direct interaction direct interaction ficd-4 1 5 unspecified role unspecified role unspecified role unspecified role Region 103-445 Region 103-445 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction