DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Internal motions prime cIAP1 for rapid activation. Internal motions prime cIAP1 for rapid activation. Nature structural & molecular biology 2014 imex curation Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. Only protein-protein interactions in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. DIP original experiment: DIP-265528X Nature structural & molecular biology 2014 IMEx Internal motions prime cIAP1 for rapid activation. Internal motions prime cIAP1 for rapid activation. Internal motions prime cIAP1 for rapid activation. Nature structural & molecular biology 2014 imex curation Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. DIP original experiment: DIP-265530X Nature structural & molecular biology 2014 IMEx Internal motions prime cIAP1 for rapid activation. Internal motions prime cIAP1 for rapid activation. Internal motions prime cIAP1 for rapid activation. Nature structural & molecular biology 2014 imex curation Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. Only protein-protein interactions in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. DIP original experiment: DIP-265527X Nature structural & molecular biology 2014 IMEx Internal motions prime cIAP1 for rapid activation. Internal motions prime cIAP1 for rapid activation. Internal motions prime cIAP1 for rapid activation. Nature structural & molecular biology 2014 imex curation Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. Only protein-protein interactions in vitro In vitro light scattering light scattering predetermined predetermined participant predetermined Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. DIP original experiment: DIP-265532X Nature structural & molecular biology 2014 IMEx Internal motions prime cIAP1 for rapid activation. Internal motions prime cIAP1 for rapid activation. Internal motions prime cIAP1 for rapid activation. Nature structural & molecular biology 2014 imex curation Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. Only protein-protein interactions in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. DIP original experiment: DIP-265531X Nature structural & molecular biology 2014 IMEx Internal motions prime cIAP1 for rapid activation. Internal motions prime cIAP1 for rapid activation. Internal motions prime cIAP1 for rapid activation. Nature structural & molecular biology 2014 imex curation Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. Only protein-protein interactions in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli weight by comassie molecular weight estimation by coomasie staining weight by comassie Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. DIP original experiment: DIP-265529X Nature structural & molecular biology 2014 IMEx Internal motions prime cIAP1 for rapid activation. ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG q13490-1 Baculoviral IAP repeat-containing protein 2 BIRC2 API1 MIHB RNF48 Cellular inhibitor of apoptosis 1 IAP homolog B Inhibitor of apoptosis protein 2 RING finger protein 48 RING-type E3 ubiquitin transferase BIRC2 TNFR2-TRAF-signaling complex protein 2 protein protein human Homo sapiens Human MHKTASQRLFPGPSYQNIKSIMEDSTILSDWTNSNKQKMKYDFSCELYRMSTYSTFPAGVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKLGDSPIQKHKQLYPSCSFIQNLVSASLGSTSKNTSPMRNSFAHSLSPTLEHSSLFSGSYSSLSPNPLNSRAVEDISSSRTNPYSYAMSTEEARFLTYHMWPLTFLSPSELARAGFYYIGPGDRVACFACGGKLSNWEPKDDAMSEHRRHFPNCPFLENSLETLRFSISNLSMQTHAARMRTFMYWPSSVPVQPEQLASAGFYYVGRNDDVKCFCCDGGLRCWESGDDPWVEHAKWFPRCEFLIRMKGQEFVDEIQGRYPHLLEQLLSTSDTTGEENADPPIIHFGPGESSSEDAVMMNTPVVKSALEMGFNRDLVKQTVQSKILTTGENYKTVNDIVSALLNAEDEKREEEKEKQAEEMASDDLSLIRKNRMALFQQLTCVLPILDNLLKANVINKQEHDIIKQKTQIPLQARELIDTILVKGNAAANIFKNCLKEIDSTLYKNLFVDKNMKYIPTEDVSGLSLEEQLRRLQEERTCKVCMDKEVSVVFIPCGHLVVCQECAPSLRKCPICRGIIKGTVRTFLS DIP protein Q13490-1 original sequence version: 154 p61077-1 Ubiquitin-conjugating enzyme E2 D3 UBE2D3 UBC5C UBCH5C (E3-independent) E2 ubiquitin-conjugating enzyme D3 E2 ubiquitin-conjugating enzyme D3 Ubiquitin carrier protein D3 Ubiquitin-conjugating enzyme E2(17)KB 3 Ubiquitin-conjugating enzyme E2-17 kDa 3 Ubiquitin-protein ligase D3 protein protein human Homo sapiens Human MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM DIP protein P61077-1 original sequence version: 76 DIP protein P61077 original sequence version: 86 DIP protein P61077 original sequence version: 120 DIP protein P61077 original sequence version: 31 birc2-ube2d3-1 3 9 unspecified role unspecified role prey prey ecolx Escherichia coli 8 unspecified role unspecified role bait bait Region 266-362/373-618 Region 266-362/373-618 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction birc2-1 6 8 unspecified role unspecified role unspecified role unspecified role Region 266-362/373-618 Region 266-362/373-618 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction birc2-2 2 8 unspecified role unspecified role unspecified role unspecified role Region 266-362/373-618 Region 266-362/373-618 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ub-birc2-1 1 7 unspecified role unspecified role bait bait ecolx Escherichia coli 8 unspecified role unspecified role prey prey Region 266-362/373-618 Region 266-362/373-618 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain Q13490-1:p.[Met402Ala;Phe404Ala] M402A/F404A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain M A This feature has been corrected as a result of our quality control procedures. The original label was 'M402A/F404A'. Wed May 02 19:59:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met402ala,phe404ala' Tue Jun 30 18:13:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Met402Ala;Phe404Ala]' ecolx Escherichia coli direct interaction direct interaction birc2-3 5 8 unspecified role unspecified role unspecified role unspecified role Region 266-362/373-618 Region 266-362/373-618 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction birc2-4 4 8 unspecified role unspecified role unspecified role unspecified role Region 266-362/373-618 Region 266-362/373-618 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction