DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. Nature 2015 imex curation Guo X., Wang L., Li J., Ding Z., Xiao J., Yin X., He S., Shi P., Dong L., Li G., Tian C., Wang J., Cong Y., Xu Y. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Guo X., Wang L., Li J., Ding Z., Xiao J., Yin X., He S., Shi P., Dong L., Li G., Tian C., Wang J., Cong Y., Xu Y. DIP original experiment: DIP-265161X Nature 2015 IMEx Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. Nature 2015 imex curation Guo X., Wang L., Li J., Ding Z., Xiao J., Yin X., He S., Shi P., Dong L., Li G., Tian C., Wang J., Cong Y., Xu Y. Only protein-protein interactions in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Guo X., Wang L., Li J., Ding Z., Xiao J., Yin X., He S., Shi P., Dong L., Li G., Tian C., Wang J., Cong Y., Xu Y. DIP original experiment: DIP-265163X Nature 2015 IMEx Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. Nature 2015 imex curation Guo X., Wang L., Li J., Ding Z., Xiao J., Yin X., He S., Shi P., Dong L., Li G., Tian C., Wang J., Cong Y., Xu Y. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Guo X., Wang L., Li J., Ding Z., Xiao J., Yin X., He S., Shi P., Dong L., Li G., Tian C., Wang J., Cong Y., Xu Y. DIP original experiment: DIP-265160X Nature 2015 IMEx Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. Nature 2015 imex curation Guo X., Wang L., Li J., Ding Z., Xiao J., Yin X., He S., Shi P., Dong L., Li G., Tian C., Wang J., Cong Y., Xu Y. Only protein-protein interactions in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Guo X., Wang L., Li J., Ding Z., Xiao J., Yin X., He S., Shi P., Dong L., Li G., Tian C., Wang J., Cong Y., Xu Y. DIP original experiment: DIP-265164X Nature 2015 IMEx Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. Nature 2015 imex curation Guo X., Wang L., Li J., Ding Z., Xiao J., Yin X., He S., Shi P., Dong L., Li G., Tian C., Wang J., Cong Y., Xu Y. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Guo X., Wang L., Li J., Ding Z., Xiao J., Yin X., He S., Shi P., Dong L., Li G., Tian C., Wang J., Cong Y., Xu Y. DIP original experiment: DIP-265157X Nature 2015 IMEx Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. Nature 2015 imex curation Guo X., Wang L., Li J., Ding Z., Xiao J., Yin X., He S., Shi P., Dong L., Li G., Tian C., Wang J., Cong Y., Xu Y. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Guo X., Wang L., Li J., Ding Z., Xiao J., Yin X., He S., Shi P., Dong L., Li G., Tian C., Wang J., Cong Y., Xu Y. DIP original experiment: DIP-265159X Nature 2015 IMEx Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. Nature 2015 imex curation Guo X., Wang L., Li J., Ding Z., Xiao J., Yin X., He S., Shi P., Dong L., Li G., Tian C., Wang J., Cong Y., Xu Y. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Guo X., Wang L., Li J., Ding Z., Xiao J., Yin X., He S., Shi P., Dong L., Li G., Tian C., Wang J., Cong Y., Xu Y. DIP original experiment: DIP-265162X Nature 2015 IMEx Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. Nature 2015 imex curation Guo X., Wang L., Li J., Ding Z., Xiao J., Yin X., He S., Shi P., Dong L., Li G., Tian C., Wang J., Cong Y., Xu Y. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Guo X., Wang L., Li J., Ding Z., Xiao J., Yin X., He S., Shi P., Dong L., Li G., Tian C., Wang J., Cong Y., Xu Y. DIP original experiment: DIP-265158X Nature 2015 IMEx Structural insight into autoinhibition and histone H3-induced activation of DNMT3A. h3(112) Histone 3 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) ARTKQTARKSTG dnm3a_human DNA (cytosine-5)-methyltransferase 3A DNMT3A DNA methyltransferase HsaIIIA Cysteine methyltransferase DNMT3A protein protein human Homo sapiens Human MPAMPSSGPGDTSSSAAEREEDRKDGEEQEEPRGKEERQEPSTTARKVGRPGRKRKHPPVESGDTPKDPAVISKSPSMAQDSGASELLPNGDLEKRSEPQPEEGSPAGGQKGGAPAEGEGAAETLPEASRAVENGCCTPKEGRGAPAEAGKEQKETNIESMKMEGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREAEKKAKVIAGMNAVEENQGPGESQKVEEASPPAVQQPTDPASPTVATTPEPVGSDAGDKNATKAGDDEPEYEDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVCVEKLMPLSSFCSAFHQATYNKQPMYRKAIYEVLQVASSRAGKLFPVCHDSDESDTAKAVEVQNKPMIEWALGGFQPSGPKGLEPPEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPRKSTAEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVTLEHPLFVGGMCQNCKNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQAAIKEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV DIP protein Q9Y6K1 original sequence version: 134 DIP protein Q9Y6K1-1 original sequence version: 88 DIP protein Q9Y6K1 original sequence version: 88 DIP protein Q9Y6K1-1 original sequence version: 135 BD1FF7C5B4F54A33 q9ujw3-1 DNA (cytosine-5)-methyltransferase 3-like DNMT3L protein protein human Homo sapiens Human MAAIPALDPEAEPSMDVILVGSSELSSSVSPGTGRDLIAYEVKANQRNIEDICICCGSLQVHTQHPLFEGGICAPCKDKFLDALFLYDDDGYQSYCSICCSGETLLICGNPDCTRCYCFECVDSLVGPGTSGKVHAMSNWVCYLCLPSSRSGLLQRRRKWRSQLKAFYDRESENPLEMFETVPVWRRQPVRVLSLFEDIKKELTSLGFLESGSDPGQLKHVVDVTDTVRKDVEEWGPFDLVYGATPPLGHTCDRPPSWYLFQFHRLLQYARPKPGSPRPFFWMFVDNLVLNKEDLDVASRFLEMEPVTIPDVHGGSLQNAVRVWSNIPAIRSRHWALVSEEELSLLAQNKQSSKLAAKWPTKLVKNCFLPLREYFKYFSTELTSSL DIP protein Q9UJW3-1 original sequence version: 123 DIP protein Q9UJW3 original sequence version: 107 dnmt3a-h3(112)-1 1 9 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 10 unspecified role unspecified role unspecified role unspecified role Region 476-912 Region 476-912 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction dnmt3a-dnmt3l-7 6 9 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 11 unspecified role unspecified role unspecified role unspecified role Region 178-379 Region 178-379 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Region 476-912 Region 476-912 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association dnmt3a-dnmt3l-8 5 11 unspecified role unspecified role unspecified role unspecified role Region 178-379 Region 178-379 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Region 455-912 Region 455-912 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction dnmt3a-dnmt3l-9 2 11 unspecified role unspecified role unspecified role unspecified role Region 178-379 Region 178-379 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Region 224-912 Region 224-912 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction dnmt3a-dnmt3l-10 8 11 unspecified role unspecified role unspecified role unspecified role Region 178-379 Region 178-379 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Region 455-912 Region 455-912 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction dnmt3a-h3(112)-2 3 9 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 10 unspecified role unspecified role unspecified role unspecified role Region 476-609 Region 476-609 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction h3(112)-dnmt3a-1 7 9 unspecified role unspecified role bait bait Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 11 unspecified role unspecified role prey prey Region 178-379 Region 178-379 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role prey prey Region 476-912 Region 476-912 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli association association dnmt3a-dnmt3l-11 4 9 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 11 unspecified role unspecified role unspecified role unspecified role Region 178-379 Region 178-379 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Region 224-912 Region 224-912 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association