DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu CEACAM1 regulates TIM-3-mediated tolerance and exhaustion. CEACAM1 regulates TIM-3-mediated tolerance and exhaustion. Nature 2015 imex curation Huang YH., Zhu C., Kondo Y., Anderson AC., Gandhi A., Russell A., Dougan SK., Petersen BS., Melum E., Pertel T., Clayton KL., Raab M., Chen Q., Beauchemin N., Yazaki PJ., Pyzik M., Ostrowski MA., Glickman JN., Rudd CE., Ploegh HL., Franke A., Petsko GA., Kuchroo VK., Blumberg RS. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Huang YH., Zhu C., Kondo Y., Anderson AC., Gandhi A., Russell A., Dougan SK., Petersen BS., Melum E., Pertel T., Clayton KL., Raab M., Chen Q., Beauchemin N., Yazaki PJ., Pyzik M., Ostrowski MA., Glickman JN., Rudd CE., Ploegh HL., Franke A., Petsko GA., Kuchroo VK., Blumberg RS. DIP original experiment: DIP-265251X Nature 2015 IMEx CEACAM1 regulates TIM-3-mediated tolerance and exhaustion. CEACAM1 regulates TIM-3-mediated tolerance and exhaustion. CEACAM1 regulates TIM-3-mediated tolerance and exhaustion. Nature 2015 imex curation Huang YH., Zhu C., Kondo Y., Anderson AC., Gandhi A., Russell A., Dougan SK., Petersen BS., Melum E., Pertel T., Clayton KL., Raab M., Chen Q., Beauchemin N., Yazaki PJ., Pyzik M., Ostrowski MA., Glickman JN., Rudd CE., Ploegh HL., Franke A., Petsko GA., Kuchroo VK., Blumberg RS. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Huang YH., Zhu C., Kondo Y., Anderson AC., Gandhi A., Russell A., Dougan SK., Petersen BS., Melum E., Pertel T., Clayton KL., Raab M., Chen Q., Beauchemin N., Yazaki PJ., Pyzik M., Ostrowski MA., Glickman JN., Rudd CE., Ploegh HL., Franke A., Petsko GA., Kuchroo VK., Blumberg RS. DIP original experiment: DIP-265249X Nature 2015 IMEx CEACAM1 regulates TIM-3-mediated tolerance and exhaustion. CEACAM1 regulates TIM-3-mediated tolerance and exhaustion. CEACAM1 regulates TIM-3-mediated tolerance and exhaustion. Nature 2015 imex curation Huang YH., Zhu C., Kondo Y., Anderson AC., Gandhi A., Russell A., Dougan SK., Petersen BS., Melum E., Pertel T., Clayton KL., Raab M., Chen Q., Beauchemin N., Yazaki PJ., Pyzik M., Ostrowski MA., Glickman JN., Rudd CE., Ploegh HL., Franke A., Petsko GA., Kuchroo VK., Blumberg RS. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Huang YH., Zhu C., Kondo Y., Anderson AC., Gandhi A., Russell A., Dougan SK., Petersen BS., Melum E., Pertel T., Clayton KL., Raab M., Chen Q., Beauchemin N., Yazaki PJ., Pyzik M., Ostrowski MA., Glickman JN., Rudd CE., Ploegh HL., Franke A., Petsko GA., Kuchroo VK., Blumberg RS. DIP original experiment: DIP-265250X Nature 2015 IMEx CEACAM1 regulates TIM-3-mediated tolerance and exhaustion. CEACAM1 regulates TIM-3-mediated tolerance and exhaustion. CEACAM1 regulates TIM-3-mediated tolerance and exhaustion. Nature 2015 imex curation Huang YH., Zhu C., Kondo Y., Anderson AC., Gandhi A., Russell A., Dougan SK., Petersen BS., Melum E., Pertel T., Clayton KL., Raab M., Chen Q., Beauchemin N., Yazaki PJ., Pyzik M., Ostrowski MA., Glickman JN., Rudd CE., Ploegh HL., Franke A., Petsko GA., Kuchroo VK., Blumberg RS. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Huang YH., Zhu C., Kondo Y., Anderson AC., Gandhi A., Russell A., Dougan SK., Petersen BS., Melum E., Pertel T., Clayton KL., Raab M., Chen Q., Beauchemin N., Yazaki PJ., Pyzik M., Ostrowski MA., Glickman JN., Rudd CE., Ploegh HL., Franke A., Petsko GA., Kuchroo VK., Blumberg RS. DIP original experiment: DIP-265247X Nature 2015 IMEx CEACAM1 regulates TIM-3-mediated tolerance and exhaustion. CEACAM1 regulates TIM-3-mediated tolerance and exhaustion. CEACAM1 regulates TIM-3-mediated tolerance and exhaustion. Nature 2015 imex curation Huang YH., Zhu C., Kondo Y., Anderson AC., Gandhi A., Russell A., Dougan SK., Petersen BS., Melum E., Pertel T., Clayton KL., Raab M., Chen Q., Beauchemin N., Yazaki PJ., Pyzik M., Ostrowski MA., Glickman JN., Rudd CE., Ploegh HL., Franke A., Petsko GA., Kuchroo VK., Blumberg RS. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Huang YH., Zhu C., Kondo Y., Anderson AC., Gandhi A., Russell A., Dougan SK., Petersen BS., Melum E., Pertel T., Clayton KL., Raab M., Chen Q., Beauchemin N., Yazaki PJ., Pyzik M., Ostrowski MA., Glickman JN., Rudd CE., Ploegh HL., Franke A., Petsko GA., Kuchroo VK., Blumberg RS. DIP original experiment: DIP-265248X Nature 2015 IMEx CEACAM1 regulates TIM-3-mediated tolerance and exhaustion. CEACAM1 regulates TIM-3-mediated tolerance and exhaustion. CEACAM1 regulates TIM-3-mediated tolerance and exhaustion. Nature 2015 imex curation Huang YH., Zhu C., Kondo Y., Anderson AC., Gandhi A., Russell A., Dougan SK., Petersen BS., Melum E., Pertel T., Clayton KL., Raab M., Chen Q., Beauchemin N., Yazaki PJ., Pyzik M., Ostrowski MA., Glickman JN., Rudd CE., Ploegh HL., Franke A., Petsko GA., Kuchroo VK., Blumberg RS. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Huang YH., Zhu C., Kondo Y., Anderson AC., Gandhi A., Russell A., Dougan SK., Petersen BS., Melum E., Pertel T., Clayton KL., Raab M., Chen Q., Beauchemin N., Yazaki PJ., Pyzik M., Ostrowski MA., Glickman JN., Rudd CE., Ploegh HL., Franke A., Petsko GA., Kuchroo VK., Blumberg RS. DIP original experiment: DIP-265246X Nature 2015 IMEx CEACAM1 regulates TIM-3-mediated tolerance and exhaustion. havr2_human Hepatitis A virus cellular receptor 2 HAVCR2 TIM3 TIMD3 T-cell immunoglobulin and mucin domain-containing protein 3 T-cell immunoglobulin mucin receptor 3 T-cell membrane protein 3 protein protein human Homo sapiens Human MFSHLPFDCVLLLLLLLLTRSSEVEYRAEVGQNAYLPCFYTPAAPGNLVPVCWGKGACPVFECGNVVLRTDERDVNYWTSRYWLNGDFRKGDVSLTIENVTLADSGIYCCRIQIPGIMNDEKFNLKLVIKPAKVTPAPTRQRDFTAAFPRMLTTRGHGPAETQTLGSLPDINLTQISTLANELRDSRLANDLRDSGATIRIGIYIGAGICAGLALALIFGALIFKWYSHSKEKIQNLSLISLANLPPSGLANAVAEGIRSEENIYTIEENVYEVEEPNEYYCYVSSRQQPSQPLGCRFAMP DIP protein Q8TDQ0 original sequence version: 100 519A5B0D512B6173 ceam1_human Carcinoembryonic antigen-related cell adhesion molecule 1 CEACAM1 BGP BGP1 Biliary glycoprotein 1 protein protein human Homo sapiens Human MGHLSAPLHRVRVPWQGLLLTASLLTFWNPPTTAQLTTESMPFNVAEGKEVLLLVHNLPQQLFGYSWYKGERVDGNRQIVGYAIGTQQATPGPANSGRETIYPNASLLIQNVTQNDTGFYTLQVIKSDLVNEEATGQFHVYPELPKPSISSNNSNPVEDKDAVAFTCEPETQDTTYLWWINNQSLPVSPRLQLSNGNRTLTLLSVTRNDTGPYECEIQNPVSANRSDPVTLNVTYGPDTPTISPSDTYYRPGANLSLSCYAASNPPAQYSWLINGTFQQSTQELFIPNITVNNSGSYTCHANNSVTGCNRTTVKTIIVTELSPVVAKPQIKASKTTVTGDKDSVNLTCSTNDTGISIRWFFKNQSLPSSERMKLSQGNTTLSINPVKREDAGTYWCEVFNPISKNQSDPIMLNVNYNALPQENGLSPGAIAGIVIGVVALVALIAVALACFLHFGKTGRASDQRDLTEHKPSVSNHTQDHSNDPPNKMNEVTYSTLNFEAQQPTQPTSASPSLTATEIIYSEVKKQ DIP protein P13688 original sequence version: 164 CAD1B2328D069AF8 ceam1_mouse Carcinoembryonic antigen-related cell adhesion molecule 1 Ceacam1 Bgp Bgp1 Biliary glycoprotein 1 Murine hepatitis virus receptor MHVR1 Biliary glycoprotein D protein protein mouse Mus musculus Mouse MELASAHLHKGQVPWGGLLLTASLLASWSPATTAEVTIEAVPPQVAEDNNVLLLVHNLPLALGAFAWYKGNTTAIDKEIARFVPNSNMNFTGQAYSGREIIYSNGSLLFQMITMKDMGVYTLDMTDENYRRTQATVRFHVHPILLKPNITSNNSNPVEGDDSVSLTCDSYTDPDNINYLWSRNGESLSEGDRLKLSEGNRTLTLLNVTRNDTGPYVCETRNPVSVNRSDPFSLNIIYGPDTPIISPSDIYLHPGSNLNLSCHAASNPPAQYFWLINEKPHASSQELFIPNITTNNSGTYTCFVNNSVTGLSRTTVKNITVLEPVTQPFLQVTNTTVKELDSVTLTCLSNDIGANIQWLFNSQSLQLTERMTLSQNNSILRIDPIKREDAGEYQCEISNPVSVRRSNSIKLDIIFDPTQGGLSDGAIAGIVIGVVAGVALIAGLAYFLYSRKSGGGSDQRDLTEHKPSTSNHNLAPSDNSPNKVDDVAYTVLNFNSQQPNRPTSAPSSPRATETVYSEVKKK DIP protein P31809 original sequence version: 135 DIP protein P31809-1 original sequence version: 146 mint Unknown. In case of murine coronavirus (MHV) infection, serves as receptor for MHV S1 spike glycoprotein 1C8F71FAC47DD54E havr2_mouse Hepatitis A virus cellular receptor 2 homolog Havcr2 Tim3 Timd3 T-cell immunoglobulin and mucin domain-containing protein 3 T-cell membrane protein 3 T-cell immunoglobulin mucin receptor 3 protein protein mouse Mus musculus Mouse MFSGLTLNCVLLLLQLLLARSLENAYVFEVGKNAYLPCSYTLSTPGALVPMCWGKGFCPWSQCTNELLRTDERNVTYQKSSRYQLKGDLNKGDVSLIIKNVTLDDHGTYCCRIQFPGLMNDKKLELKLDIKAAKVTPAQTAHGDSTTASPRTLTTERNGSETQTLVTLHNNNGTKISTWADEIKDSGETIRTAIHIGVGVSAGLTLALIIGVLILKWYSCKKKKLSSLSLITLANLPPGGLANAGAVRIRSEENIYTIEENVYEVENSNEYYCYVNSQQPS DIP protein Q8VIM0 original sequence version: 99 C0349E4BD0E5761D bag6_mouse Large proline-rich protein BAG6 HLA-B-associated transcript 3 Bat3 BCL2-associated athanogene 6 Bag6 BAG family molecular chaperone regulator 6 Protein Scythe protein protein mouse Mus musculus Mouse MEPSDSASTAMEEPDSLEVLVKTLDSQTRTFIVGAQMNVKEFKEHIAASVSIPSEKQRLIYQGRVLQDDKKLQEYNVGGKVIHLVERAPPQTQLPSGASSGTGSASATHGGAPLPGTRGPGASVHDRNANSYVMVGTFNLPSDGSAVDVHINMEQAPIQSEPRVRLVMAQHMIRDIQTLLSRMECRGGTQAQASQPPPQTPQTVASETVALNSQTSEPVESEAPPREPMESEEMEERPPTQTPELAPSGPAPAGPAPAGPAPAPETNAPNHPSPAEHVEVLQELQRLQRRLQPFLQRYCEVLGAAATTDYNNNHEGREEDQRLINLVGESLRLLGNTFVALSDLRCNLACAPPRHLHVVRPMSHYTTPMVLQQAAIPIQINVGTTVTMTGNGARPPPAPGAEAATPGSAQATSLPPSSTTVDSSTEGAPPPGPAPPPASSHPRVIRISHQSVEPVVMMHMNIQDSGAQPGGVPSAPTGPLGPPGHGQTLGQQVPGFPTAPTRVVIARPTPPQARPSHPGGPPVSGALQGAGLGTNTSLAQMVSGLVGQLLMQPVLVAQGTPGMAQAQAQAQAQAQAQAQAPAPAPAPAPAPATASASAGTTNTATTAGPAPGGPAQPPPPQPSAADLQFSQLLGNLLGPAGPGAGGPGMASPTITVAMPGVPAFLQGMTDFLQASQTAPPPPPPPPPPPPAPEQQSTPPPGSPSGGTASPGGLGPESLPPEFFTSVVQGVLSSLLGSLGARAGSSESIAAFIQRLSGSSNIFEPGADGALGFFGALLSLLCQNFSMVDVVMLLHGHFQPLQRLQPQLRSFFHQHYLGGQEPTPSNIRMATHTLITGLEEYVRESFSLVQVQPGVDIIRTNLEFLQEQFNSIAAHVLHCTDSGFGARLLELCNQGLFECLALNLHCLGGQQMELAAVINGRIRRMSRGVNPSLVSWLTTMMGLRLQVVLEHMPVGPDAILRYVRRVGDPPQTLPEEPMEVQGAERTSPEPQRENASPAPGTTAEEAMSRGPPPAPEGGSRDEQDGASADAEPWAAAVPPEWVPIIQQDIQSQRKVKPQPPLSDAYLSGMPAKRRKTMQGEGPQLLLSEAVSRAAKAAGARPLTSPESLSRDLEAPEVQESYRQQLRSDIQKRLQEDPNYSPQRFPNAHRAFADDP DIP protein Q9Z1R2 original sequence version: 104 7F3FD14DF5AC1211 havcr2-bag6-1 6 11 unspecified role unspecified role prey prey mouse Mus musculus Mouse 10 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association havcr2-ceacam1-1 5 8 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range P13688:p.Gln123His Q123H mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Q H This feature has been corrected as a result of our quality control procedures. The original label was 'Q123H'. Wed May 02 19:16:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln123his' Tue Jun 30 18:25:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln123His' P13688:p.Gln78Leu Q78L mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Q L This feature has been corrected as a result of our quality control procedures. The original label was 'Q78L'. Wed May 02 16:01:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln78leu' Tue Jun 30 18:43:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln78Leu' P13688:p.Gly81Ala G81A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain G A This feature has been corrected as a result of our quality control procedures. The original label was 'G81A'. Wed May 02 14:26:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly81ala' Tue Jun 30 19:33:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly81Ala' P13688:p.Asn76Ala N76A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain N A This feature has been corrected as a result of our quality control procedures. The original label was 'N76A'. Wed May 02 17:17:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn76ala' Tue Jun 30 19:44:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn76Ala' human Homo sapiens Human 7 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association havcr2-ceacam1-2 4 8 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 7 unspecified role unspecified role bait bait Region 1-105 Region 1-105 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association havcr2-ceacam1-3 3 8 unspecified role unspecified role prey prey human Homo sapiens Human 7 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association ceacam1-havcr2-1 2 8 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 7 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q8TDQ0:p.Glu62Ala E62A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E62A'. Wed May 02 17:05:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu62ala' Tue Jun 30 17:26:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu62Ala' Q8TDQ0:p.Arg111Trp R111W mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R W This feature has been corrected as a result of our quality control procedures. The original label was 'R111W'. Wed May 02 18:00:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg111trp' Tue Jun 30 15:54:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg111Trp' Q8TDQ0:p.Cys58Arg C58R mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain C R This feature has been corrected as a result of our quality control procedures. The original label was 'C58R'. Wed May 02 16:29:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys58arg' Tue Jun 30 17:22:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys58Arg' Q8TDQ0:p.Cys110Ala C110A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain C A This feature has been corrected as a result of our quality control procedures. The original label was 'C110A'. Wed May 02 16:12:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys110ala' Tue Jun 30 18:56:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys110Ala' Q8TDQ0:p.Cys109Ala C109A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain C A This feature has been corrected as a result of our quality control procedures. The original label was 'C109A'. Wed May 02 14:53:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys109ala' Tue Jun 30 17:42:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys109Ala' human Homo sapiens Human physical association physical association ceacam1-havcr2-2 1 9 unspecified role unspecified role bait bait mouse Mus musculus Mouse 10 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association