<?xml version='1.0' encoding='UTF-8'?>
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  <entry>
    <source releaseDate="2022-02-03">
      <names>
        <shortLabel>DIP</shortLabel>
        <fullName>Database of Interacting Proteins</fullName>
      </names>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="identity" refTypeAc="MI:0356"/>
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      </xref>
      <attributeList>
        <attribute name="postaladdress">611 Young Drive East; Los Angeles CA 90095; USA</attribute>
        <attribute name="url" nameAc="MI:0614">http://dip.doe-mbi.ucla.edu/</attribute>
        <attribute name="contact-email" nameAc="MI:0634">dip@mbi.ucla.edu</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>A breast cancer stem cell niche supported by juxtacrine signalling from monocytes and macrophages.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="25266422" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="doi" dbAc="MI:0574" id="10.1038/ncb3041" refType="primary-reference" refTypeAc="MI:0358"/>
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            <secondaryRef db="imex" dbAc="MI:0670" id="IM-23373" refType="imex-primary" refTypeAc="MI:0662"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">A breast cancer stem cell niche supported by juxtacrine signalling from monocytes and macrophages.</attribute>
            <attribute name="journal" nameAc="MI:0885">Nature cell biology</attribute>
            <attribute name="publication year" nameAc="MI:0886">2014</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Lu H.,  Clauser KR.,  Tam WL.,  Fröse J.,  Ye X.,  Eaton EN.,  Reinhardt F.,  Donnenberg VS.,  Bhargava R.,  Carr SA.,  Weinberg RA.</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="imex" dbAc="MI:0670" id="IM-23373" refType="imex-primary" refTypeAc="MI:0662"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-262413X" refType="identity" refTypeAc="MI:0356"/>
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          <secondaryRef db="doi" dbAc="MI:0574" id="10.1038/ncb3041" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="9606">
            <names>
              <shortLabel>human</shortLabel>
              <fullName>Homo sapiens</fullName>
              <alias type="synonym" typeAc="MI:1041">Human</alias>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>anti bait coip</shortLabel>
            <fullName>anti bait coimmunoprecipitation</fullName>
            <alias type="synonym" typeAc="MI:1041">anti bait coip</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0006" refType="identity" refTypeAc="MI:0356"/>
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          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>western blot</shortLabel>
            <fullName>western blot</fullName>
            <alias type="go synonym" typeAc="MI:0303">Immuno blot</alias>
            <alias type="synonym" typeAc="MI:1041">Immuno blot</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0113" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-973" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Lu H., Clauser KR., Tam WL., Fröse J., Ye X., Eaton EN., Reinhardt F., Donnenberg VS., Bhargava R., Carr SA., Weinberg RA.</attribute>
          <attribute name="comment" nameAc="MI:0612">DIP original experiment: DIP-262413X</attribute>
          <attribute name="journal" nameAc="MI:0885">Nature cell biology</attribute>
          <attribute name="publication year" nameAc="MI:0886">2014</attribute>
          <attribute name="curation depth" nameAc="MI:0955">IMEx</attribute>
          <attribute name="title" nameAc="MI:1091">A breast cancer stem cell niche supported by juxtacrine signalling from monocytes and macrophages.</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>nfkb1_human</shortLabel>
          <fullName>Nuclear factor NF-kappa-B p105 subunit</fullName>
          <alias type="gene name" typeAc="MI:0301">NFKB1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">DNA-binding factor KBF1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">EBP-1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="P19838" version="SP_113" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSP00000378297" version="SP_257" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSP00000424790" version="SP_257" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q68D84" version="SP_113" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q86V43" version="SP_113" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q8N4X7" version="SP_113" refType="secondary-ac" refTypeAc="MI:0360"/>
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          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-300010" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-5603029" version="SP_257"/>
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          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-844456" version="SP_257"/>
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          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-933542" version="SP_257"/>
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          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENST00000394820" version="SP_257" refType="transcript"/>
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          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR020683" version="SP_257"/>
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          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR033926" version="SP_257"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR036770" version="SP_257"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR037059" version="SP_257"/>
          <secondaryRef db="mint" dbAc="MI:0471" id="P19838" version="SP_257"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1MDI" version="SP_257"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1MDJ" version="SP_257"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1MDK" version="SP_257"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1NFI" version="SP_257"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1SVC" version="SP_257"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2DBF" version="SP_257"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2O61" version="SP_257"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3GUT" version="SP_257"/>
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          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-445989" version="SP_257"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_001158884.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_003989.2"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_001306155.1"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-106N"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="9606">
          <names>
            <shortLabel>human</shortLabel>
            <fullName>Homo sapiens</fullName>
            <alias type="synonym" typeAc="MI:1041">Human</alias>
          </names>
        </organism>
        <sequence>MAEDDPYLGRPEQMFHLDPSLTHTIFNPEVFQPQMALPTDGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGASSEKNKKSYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGICTVTAGPKDMVVGFANLGILHVTKKKVFETLEARMTEACIRGYNPGLLVHPDLAYLQAEGGGDRQLGDREKELIRQAALQQTKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDINITKPASVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGGTGSTGPGYSFPHYGFPTYGGITFHPGTTKSNAGMKHGTMDTESKKDPEGCDKSDDKNTVNLFGKVIETTEQDQEPSEATVGNGEVTLTYATGTKEESAGVQDNLFLEKAMQLAKRHANALFDYAVTGDVKMLLAVQRHLTAVQDENGDSVLHLAIIHLHSQLVRDLLEVTSGLISDDIINMRNDLYQTPLHLAVITKQEDVVEDLLRAGADLSLLDRLGNSVLHLAAKEGHDKVLSILLKHKKAALLLDHPNGDGLNAIHLAMMSNSLPCLLLLVAAGADVNAQEQKSGRTALHLAVEHDNISLAGCLLLEGDAHVDSTTYDGTTPLHIAAGRGSTRLAALLKAAGADPLVENFEPLYDLDDSWENAGEDEGVVPGTTPLDMATSWQVFDILNGKPYEPEFTSDDLLAQGDMKQLAEDVKLQLYKLLEIPDPDKNWATLAQKLGLGILNNAFRLSPAPSKTLMDNYEVSGGTVRELVEALRQMGYTEAIEVIQAASSPVKTTSQAHSLPLSPASTRQQIDELRDSDSVCDSGVETSFRKLSFTESLTSGASLLTLNKMPHDYGQEGPLEGKI</sequence>
        <attributeList>
          <attribute name="remark-internal">DIP protein P19838 original sequence version: 202</attribute>
          <attribute name="remark-internal">DIP protein P19838 original sequence version: 192</attribute>
          <attribute name="remark-internal">DIP protein P19838 original sequence version: 195</attribute>
          <attribute name="crc64">2A7C8FF86A10D1A8</attribute>
        </attributeList>
      </interactor>
      <interactor id="3">
        <names>
          <shortLabel>twst1_human</shortLabel>
          <fullName>Twist-related protein 1</fullName>
          <alias type="gene name synonym" typeAc="MI:0302">H-twist</alias>
          <alias type="gene name" typeAc="MI:0301">TWIST1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">TWIST</alias>
          <alias type="gene name synonym" typeAc="MI:0302">BHLHA38</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Class A basic helix-loop-helix protein 38</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="Q15672" version="SP_90" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q92487" version="SP_90" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q99804" version="SP_90" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="A4D128" version="SP_95" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSP00000242261" version="SP_213" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1797287" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_000465.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="XP_011513798.1"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-45974N"/>
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          <secondaryRef db="go" dbAc="MI:0448" id="GO:2000802" version="SP_213"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR011598" version="SP_213"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR015789" version="SP_213"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR036638" version="SP_213"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2MJV" version="SP_213"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-6785807" version="SP_213"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-8878166" version="SP_213"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-8939902" version="SP_213"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="9606">
          <names>
            <shortLabel>human</shortLabel>
            <fullName>Homo sapiens</fullName>
            <alias type="synonym" typeAc="MI:1041">Human</alias>
          </names>
        </organism>
        <sequence>MMQDVSSSPVSPADDSLSNSEEEPDRQQPPSGKRGGRKRRSSRRSAGGGAGPGGAAGGGVGGGDEPGSPAQGKRGKKSAGCGGGGGAGGGGGSSSGGGSPQSYEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDELDSKMASCSYVAHERLSYAFSVWRMEGAWSMSASH</sequence>
        <attributeList>
          <attribute name="remark-internal">DIP protein Q15672 original sequence version: 156</attribute>
          <attribute name="remark-internal">DIP protein Q15672 original sequence version: 159</attribute>
          <attribute name="crc64">9394E4351BA1D081</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="4" imexId="IM-23373-1">
        <names>
          <shortLabel>twist1-nfkb1-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="dip" dbAc="MI:0465" id="DIP-197385E" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-16123040" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="imex source"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-23373-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="5">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>prey</shortLabel>
                  <fullName>prey</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0498" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-58" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="9606">
                <names>
                  <shortLabel>human</shortLabel>
                  <fullName>Homo sapiens</fullName>
                  <alias type="synonym" typeAc="MI:1041">Human</alias>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
          <participant id="6">
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>bait</shortLabel>
                  <fullName>bait</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0496" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-49" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="9606">
                <names>
                  <shortLabel>human</shortLabel>
                  <fullName>Homo sapiens</fullName>
                  <alias type="synonym" typeAc="MI:1041">Human</alias>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
      </interaction>
    </interactionList>
  </entry>
</entrySet>