IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ De novo TBR1 mutations in sporadic autism disrupt protein functions. De novo TBR1 mutations in sporadic autism disrupt protein functions. Nature communications 2014 imex curation Deriziotis P., O'Roak BJ., Graham SA., Estruch SB., Dimitropoulou D., Bernier RA., Gerdts J., Shendure J., Eichler EE., Fisher SE. Fig 6A gives Ref 19 (Sakai et al) for isoforms of FOXP2 - however NO trace of them there. [Ref 40 states:-Exon 10+ was detected in five independent clones from three different sources of cDNA (Fig. 1B) and encodes ten additional amino acids not present in the published exon 10 (Fig. 3)] Fig 3 gives total length and sequence of C-terminus of isoform 10+.] The isoforms of FOXP2, as given in figure 6A, have been assigned Uniprot identifiers as follows:- Isoform 1 =O15409-1, isoform III as O15409-2, isoform 10+ (FOXP2-S) as O15409-6 (see Ref 40- PMID:12189486). simon.fisher@mpi.nl 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret tag visualisation tag visualisation Deriziotis P., O'Roak BJ., Graham SA., Estruch SB., Dimitropoulou D., Bernier RA., Gerdts J., Shendure J., Eichler EE., Fisher SE. This experiment relates to a negative interaction Fig 6A gives Ref 19 (Sakai et al) for isoforms of FOXP2 - however NO trace of them there. [Ref 40 states:-Exon 10+ was detected in five independent clones from three different sources of cDNA (Fig. 1B) and encodes ten additional amino acids not present in the published exon 10 (Fig. 3)] Fig 3 gives total length and sequence of C-terminus of isoform 10+.] The isoforms of FOXP2, as given in figure 6A, have been assigned Uniprot identifiers as follows:- Isoform 1 =O15409-1, isoform III as O15409-2, isoform 10+ (FOXP2-S) as O15409-6 (see Ref 40- PMID:12189486). simon.fisher@mpi.nl Accepted 2018-JUN-05 AT 09:53 BST AT 09:53 BST by PPORRAS Nature communications 2014 Only protein-protein interactions imex curation imex curation I have removed "Supp fig 6B" from EBI-16628317 as this figure is TBR1 interacting with TBR1, so new interaction created for SUpp Fig 6B. EBI-16628317 is TBR1 with FOXP2. I have removed mutations associated with the wrong protein.; altered; EBI-16595119 is currently labelled Supp figure 6B - which is for a different cell line (293), and I have already re-created this in Experiment-3. I have altered the figure legend to Supp 7 and removed the two TBR1 mutations. De novo TBR1 mutations in sporadic autism disrupt protein functions. De novo TBR1 mutations in sporadic autism disrupt protein functions. Nature communications 2014 imex curation Deriziotis P., O'Roak BJ., Graham SA., Estruch SB., Dimitropoulou D., Bernier RA., Gerdts J., Shendure J., Eichler EE., Fisher SE. Fig 6A gives Ref 19 (Sakai et al) for isoforms of FOXP2 - however NO trace of them there. [Ref 40 states:-Exon 10+ was detected in five independent clones from three different sources of cDNA (Fig. 1B) and encodes ten additional amino acids not present in the published exon 10 (Fig. 3)] Fig 3 gives total length and sequence of C-terminus of isoform 10+.] The isoforms of FOXP2, as given in figure 6A, have been assigned Uniprot identifiers as follows:- Isoform 1 =O15409-1, isoform III as O15409-2, isoform 10+ (FOXP2-S) as O15409-6 (see Ref 40- PMID:12189486). simon.fisher@mpi.nl 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Deriziotis P., O'Roak BJ., Graham SA., Estruch SB., Dimitropoulou D., Bernier RA., Gerdts J., Shendure J., Eichler EE., Fisher SE. This experiment relates to a negative interaction Fig 6A gives Ref 19 (Sakai et al) for isoforms of FOXP2 - however NO trace of them there. [Ref 40 states:-Exon 10+ was detected in five independent clones from three different sources of cDNA (Fig. 1B) and encodes ten additional amino acids not present in the published exon 10 (Fig. 3)] Fig 3 gives total length and sequence of C-terminus of isoform 10+.] The isoforms of FOXP2, as given in figure 6A, have been assigned Uniprot identifiers as follows:- Isoform 1 =O15409-1, isoform III as O15409-2, isoform 10+ (FOXP2-S) as O15409-6 (see Ref 40- PMID:12189486). simon.fisher@mpi.nl Accepted 2018-JUN-05 AT 09:53 BST AT 09:53 BST by PPORRAS Nature communications 2014 Only protein-protein interactions imex curation imex curation I have removed "Supp fig 6B" from EBI-16628317 as this figure is TBR1 interacting with TBR1, so new interaction created for SUpp Fig 6B. EBI-16628317 is TBR1 with FOXP2. I have removed mutations associated with the wrong protein.; altered; EBI-16595119 is currently labelled Supp figure 6B - which is for a different cell line (293), and I have already re-created this in Experiment-3. I have altered the figure legend to Supp 7 and removed the two TBR1 mutations. De novo TBR1 mutations in sporadic autism disrupt protein functions. De novo TBR1 mutations in sporadic autism disrupt protein functions. Nature communications 2014 imex curation Deriziotis P., O'Roak BJ., Graham SA., Estruch SB., Dimitropoulou D., Bernier RA., Gerdts J., Shendure J., Eichler EE., Fisher SE. Fig 6A gives Ref 19 (Sakai et al) for isoforms of FOXP2 - however NO trace of them there. [Ref 40 states:-Exon 10+ was detected in five independent clones from three different sources of cDNA (Fig. 1B) and encodes ten additional amino acids not present in the published exon 10 (Fig. 3)] Fig 3 gives total length and sequence of C-terminus of isoform 10+.] The isoforms of FOXP2, as given in figure 6A, have been assigned Uniprot identifiers as follows:- Isoform 1 =O15409-1, isoform III as O15409-2, isoform 10+ (FOXP2-S) as O15409-6 (see Ref 40- PMID:12189486). simon.fisher@mpi.nl 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Deriziotis P., O'Roak BJ., Graham SA., Estruch SB., Dimitropoulou D., Bernier RA., Gerdts J., Shendure J., Eichler EE., Fisher SE. This experiment relates to a negative interaction Fig 6A gives Ref 19 (Sakai et al) for isoforms of FOXP2 - however NO trace of them there. [Ref 40 states:-Exon 10+ was detected in five independent clones from three different sources of cDNA (Fig. 1B) and encodes ten additional amino acids not present in the published exon 10 (Fig. 3)] Fig 3 gives total length and sequence of C-terminus of isoform 10+.] The isoforms of FOXP2, as given in figure 6A, have been assigned Uniprot identifiers as follows:- Isoform 1 =O15409-1, isoform III as O15409-2, isoform 10+ (FOXP2-S) as O15409-6 (see Ref 40- PMID:12189486). simon.fisher@mpi.nl Accepted 2018-JUN-05 AT 09:53 BST AT 09:53 BST by PPORRAS Nature communications 2014 Only protein-protein interactions imex curation imex curation I have removed "Supp fig 6B" from EBI-16628317 as this figure is TBR1 interacting with TBR1, so new interaction created for SUpp Fig 6B. EBI-16628317 is TBR1 with FOXP2. I have removed mutations associated with the wrong protein.; altered; EBI-16595119 is currently labelled Supp figure 6B - which is for a different cell line (293), and I have already re-created this in Experiment-3. I have altered the figure legend to Supp 7 and removed the two TBR1 mutations. o15409-6 Forkhead box protein P2 CAG repeat protein 44 Trinucleotide repeat-containing gene 10 protein FOXP2-S FOXP2 CAGH44 TNRC10 protein protein human Homo sapiens Human MMQESATETISNSSMNQNGMSTLSSQLDAGSRDGRSSGDTSSEVSTVELLHLQQQQALQAARQLLLQQQTSGLKSPKSSDKQRPLQVPVSVAMMTPQVITPQQMQQILQQQVLSPQQLQALLQQQQAVMLQQQQLQEFYKKQQEQLHLQLLQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQHPGKQAKEQQQQQQQQQQLAAQQLVFQQQLLQMQQLQQQQHLLSLQRQGLISIPPGQAALPVQSLPQAGLSPAEIQQLWKEVTGVHSMEDNGIKHGGLDLTTNNSSSTTSSNTSKASPPITHHSIVNGQSSVLSARRDSSSHEETGASHTLYGHGVCKWPGCESICEDFGQFLKHLNNEHALDDRSTAQCRVQMQVVQQLEIQLSKERERLQAMMTHLHMRPSEPKPSPKPVSAYCFINSK 42BC7890ACAF498C tbr1_human T-box brain protein 1 TES-56 TBR1 protein protein human Homo sapiens Human MQLEHCLSPSIMLSKKFLNVSSSYPHSGGSELVLHDHPIISTTDNLERSSPLKKITRGMTNQSDTDNFPDSKDSPGDVQRSKLSPVLDGVSELRHSFDGSAADRYLLSQSSQPQSAATAPSAMFPYPGQHGPAHPAFSIGSPSRYMAHHPVITNGAYNSLLSNSSPQGYPTAGYPYPQQYGHSYQGAPFYQFSSTQPGLVPGKAQVYLCNRPLWLKFHRHQTEMIITKQGRRMFPFLSFNISGLDPTAHYNIFVDVILADPNHWRFQGGKWVPCGKADTNVQGNRVYMHPDSPNTGAHWMRQEISFGKLKLTNNKGASNNNGQMVVLQSLHKYQPRLHVVEVNEDGTEDTSQPGRVQTFTFPETQFIAVTAYQNTDITQLKIDHNPFAKGFRDNYDTIYTGCDMDRLTPSPNDSPRSQIVPGARYAMAGSFLQDQFVSNYAKARFHPGAGAGPGPGTDRSVPHTNGLLSPQQAEDPGAPSPQRWFVTPANNRLDFAASAYDTATDFAGNAATLLSYAAAGVKALPLQAAGCTGRPLGYYADPSGWGARSPPQYCGTKSGSVLPCWPNSAAAAARMAGANPYLGEEAEGLAAERSPLPPGAAEDAKPKDLSDSSWIETPSSIKSIDSSDSGIYEQAKRRRISPADTPVSESSSPLKSEVLAQRDCEKNCAKDISGYYGFYSHS E1C8D84206EFBBB5 foxp2-tbr1-2 1 4 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell n_terminus renilla luciferase renilla luciferase protein tag renilla luciferase n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association true 6B-3 foxp2-tbr1-5 3 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization true 6C-3 foxp2-tbr1-9 2 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization true Supplementary fig 7